##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936131.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	282971
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91051379823374	31.0	31.0	34.0	30.0	34.0
2	32.034187248870026	33.0	31.0	34.0	30.0	34.0
3	31.97205013941358	33.0	31.0	34.0	30.0	34.0
4	35.69931194362673	37.0	35.0	37.0	35.0	37.0
5	31.21405020302434	37.0	35.0	37.0	0.0	37.0
6	33.4133921850649	37.0	35.0	37.0	17.0	37.0
7	29.428231161497113	35.0	32.0	37.0	0.0	37.0
8	32.54928243530256	35.0	33.0	37.0	17.0	37.0
9	36.37340575536009	39.0	35.0	39.0	32.0	39.0
10	36.778585084690654	38.0	35.0	39.0	33.0	39.0
11	36.95978033084663	39.0	37.0	39.0	33.0	39.0
12	36.90544967505504	39.0	35.0	39.0	33.0	39.0
13	36.68332090567585	39.0	35.0	39.0	33.0	39.0
14	37.904237536708706	40.0	37.0	41.0	33.0	41.0
15	38.03044481589986	40.0	37.0	41.0	33.0	41.0
16	38.1041096084051	40.0	37.0	41.0	33.0	41.0
17	38.07739662368228	40.0	37.0	41.0	33.0	41.0
18	37.98208650356397	40.0	36.0	41.0	33.0	41.0
19	37.922702326386805	40.0	36.0	41.0	33.0	41.0
20	37.810860476868655	40.0	36.0	41.0	33.0	41.0
21	37.720996144481234	39.0	36.0	41.0	33.0	41.0
22	37.65872121171427	39.0	36.0	41.0	33.0	41.0
23	37.63702994299769	39.0	36.0	41.0	33.0	41.0
24	37.599824717020475	39.0	36.0	41.0	33.0	41.0
25	37.46016376236434	39.0	35.0	41.0	33.0	41.0
26	37.312788236250356	39.0	35.0	41.0	32.0	41.0
27	37.26885440557513	39.0	35.0	41.0	32.0	41.0
28	36.983920613773144	39.0	35.0	41.0	31.0	41.0
29	36.904912517537134	39.0	35.0	41.0	31.0	41.0
30	36.77468715875479	39.0	35.0	41.0	31.0	41.0
31	36.684674401263734	39.0	35.0	41.0	31.0	41.0
32	36.66434369599712	39.0	35.0	41.0	30.0	41.0
33	36.46324535023024	39.0	35.0	40.0	30.0	41.0
34	36.33664227076273	39.0	35.0	40.0	30.0	41.0
35	36.28841471387528	39.0	35.0	40.0	30.0	41.0
36	36.17404610366433	39.0	35.0	40.0	30.0	41.0
37	36.09241583059748	39.0	35.0	40.0	29.0	41.0
38	35.96312696354043	38.0	35.0	40.0	29.0	41.0
39	35.98020291832025	39.0	35.0	40.0	29.0	41.0
40	35.87587774012178	38.0	35.0	40.0	28.0	41.0
41	35.75084725996657	38.0	35.0	40.0	28.0	41.0
42	35.736001215672275	38.0	35.0	40.0	28.0	41.0
43	35.5784621038905	38.0	35.0	40.0	27.0	41.0
44	35.4152015577568	38.0	34.0	40.0	27.0	41.0
45	35.33052857006548	38.0	34.0	40.0	26.0	41.0
46	35.188952224786284	38.0	34.0	40.0	26.0	41.0
47	35.1257655378113	38.0	34.0	40.0	26.0	41.0
48	35.00669679931866	38.0	34.0	40.0	25.0	41.0
49	34.74790702934223	38.0	34.0	40.0	24.0	41.0
50	34.5380940096335	37.0	34.0	40.0	24.0	41.0
51	32.394581070144994	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	9.0
10	18.0
11	16.0
12	10.0
13	4.0
14	7.0
15	14.0
16	33.0
17	78.0
18	133.0
19	326.0
20	597.0
21	903.0
22	1282.0
23	1622.0
24	2015.0
25	2363.0
26	2842.0
27	3410.0
28	3866.0
29	4574.0
30	5978.0
31	7659.0
32	9956.0
33	14724.0
34	21281.0
35	26017.0
36	28668.0
37	43289.0
38	53008.0
39	48262.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68448356898764	20.562177749663395	26.25887458432136	11.494464097027612
2	31.073502231677452	25.419919355693693	25.742567259542497	17.764011153086358
3	32.531248785211204	24.64598845818123	26.446172929381458	16.376589827226113
4	29.18037537415495	26.568800336430236	24.92092829300529	19.329895996409526
5	21.947832110004207	39.979361842733	21.24705358499634	16.82575246226645
6	24.917394361966423	36.25777906569931	23.998925684964185	14.825900887370084
7	69.00318407186602	20.174858907803273	6.634602132373988	4.187354887956716
8	82.49573277827056	5.374755717016938	7.626576574984716	4.502934929727781
9	75.66923819048596	8.746832714306413	9.94660230200268	5.6373267932049576
10	41.18301875457202	28.735453456361252	16.204840778737044	13.87668701032968
11	31.410992645889507	27.255796530386505	23.861102374448265	17.47210844927572
12	29.938756975096386	22.751448028243175	29.09874156715706	18.211053429503377
13	25.449604376420197	25.980754211562317	28.823448339229106	19.74619307278838
14	19.770930590060466	30.577691706924032	27.733937399945578	21.917440303069927
15	18.871545140668125	26.77376833668468	35.22587120234936	19.12881532029784
16	20.562884535871166	25.690265080167222	32.9464856822784	20.80036470168321
17	21.287693791943344	24.295775892229237	28.506454725042495	25.91007559078492
18	23.493926939509702	25.84399108035806	29.48394005039386	21.178141929738384
19	25.788508363047804	27.27028564764587	26.534167812249308	20.407038177057014
20	27.317640323566728	25.52770425237922	26.214700446335492	20.939954977718564
21	25.5796530386506	26.844093564358186	27.531089758314458	20.045163638676755
22	21.735442854568134	26.94728435069318	26.033409784041474	25.28386301069721
23	21.79127896498228	27.689763261959705	27.33106926151443	23.187888511543587
24	22.442582455445965	25.604037162818805	28.943601994550676	23.00977838718455
25	21.22160928151648	26.716518653854987	27.589046227351922	24.472825837276613
26	20.256846107905048	28.277102600619852	28.381353566266508	23.084697725208592
27	21.68738139243951	27.95551487608271	28.48277738708207	21.874326344395715
28	21.001445377794898	27.233532764841627	30.658972120818035	21.10604973654544
29	21.190157295270538	26.19349686010227	30.460718589537443	22.155627255089744
30	22.915775821550618	26.12741234967541	29.281445801866624	21.67536602690735
31	23.750136939827758	27.733937399945578	26.06627534270296	22.4496503175237
32	24.172441698972687	28.094751759014176	26.741962957334852	20.99084358467829
33	23.052538952754876	28.378526421435414	27.7035455930113	20.865389032798415
34	22.73589873167215	27.18794505444021	28.21773255916684	21.858423654720802
35	22.742259807542116	25.22873368649084	28.255545621282746	23.773460884684297
36	22.867007573214217	27.378777330539172	29.462383071056752	20.29183202518986
37	22.916482607758393	29.180021981051063	28.184867000505353	19.718628410685195
38	22.60054917288344	27.888723579448072	26.068395701326285	23.442331546342203
39	23.690766898374747	26.489640281159556	27.51836760657452	22.301225213891176
40	23.296026801332996	25.296231769333254	29.729901650699187	21.67783977863456
41	19.362408161967124	26.346869467189215	28.84465192546233	25.44607044538133
42	22.628113834986625	26.522505839821044	27.33495658565719	23.514423739535147
43	23.425368677355628	26.098080722052792	27.455110240978758	23.02144035961282
44	22.27401394489188	27.0119552887045	27.39079269607133	23.323238070332295
45	21.845348109876984	26.387863067240108	26.77023440564581	24.996554417237103
46	22.017097158366052	27.154372709570946	27.88306928978588	22.945460842277125
47	21.450608012835236	26.384682529305124	29.475458615900568	22.68925084195907
48	21.282746288488926	24.97605761721166	28.96798611871888	24.77320997558054
49	21.018054853677583	25.567637673118448	29.221015581101952	24.193291892102017
50	20.796477377540455	24.66542507889501	30.054669913171317	24.48342763039322
51	20.508108604768687	25.08419590700107	27.853384269059372	26.554311219170867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	300.0
1	485.5
2	671.0
3	855.5
4	1040.0
5	859.0
6	678.0
7	605.0
8	532.0
9	554.5
10	577.0
11	590.5
12	604.0
13	583.5
14	563.0
15	569.0
16	575.0
17	587.0
18	599.0
19	754.0
20	909.0
21	1092.5
22	1276.0
23	1331.0
24	1386.0
25	1728.0
26	2403.5
27	2737.0
28	3405.0
29	4073.0
30	4612.0
31	5151.0
32	5986.5
33	6822.0
34	7066.5
35	7311.0
36	7894.0
37	8477.0
38	10007.5
39	11538.0
40	13308.5
41	15079.0
42	17897.0
43	20715.0
44	23480.5
45	26246.0
46	26758.5
47	27271.0
48	26001.0
49	24731.0
50	21880.5
51	19030.0
52	16871.5
53	14713.0
54	13976.0
55	13239.0
56	12831.0
57	12423.0
58	12380.0
59	12337.0
60	11905.0
61	11473.0
62	10633.0
63	9793.0
64	8192.0
65	6591.0
66	5349.5
67	4108.0
68	3227.0
69	2346.0
70	2118.0
71	1890.0
72	1605.5
73	1321.0
74	1016.0
75	592.0
76	473.0
77	367.0
78	261.0
79	198.0
80	135.0
81	108.5
82	82.0
83	65.0
84	48.0
85	36.5
86	25.0
87	20.5
88	16.0
89	15.5
90	15.0
91	11.5
92	8.0
93	4.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	282971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.7349198191117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73688991702707	26.31515949453236
2	10.671033121870503	8.053411584817372
3	4.79797496047079	5.431536012824127
4	2.9742150442621287	4.489270648801087
5	1.96208470944764	3.701955459465588
6	1.47673799465974	3.3434753893392664
7	1.067431579370291	2.8195611541947994
8	0.8344225406230582	2.518949413253646
9	0.6322882523850253	2.1473411855683673
>10	5.774233648632072	38.6735455132055
>50	0.05775229332325619	1.471388494211387
>100	0.014935937928428324	1.0344056497865037
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	423	0.14948528294418864	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	331	0.1169731173865873	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5339310388697077E-4	0.0	0.0	0.12651473119153553	0.0
2	3.5339310388697077E-4	0.0	0.0	0.6417618766587389	0.0
3	3.5339310388697077E-4	0.0	0.0	0.8718207872891568	0.0
4	3.5339310388697077E-4	0.0	0.0	1.1403995462432546	0.0
5	3.5339310388697077E-4	0.0	0.0	1.96804619554654	0.0
6	3.5339310388697077E-4	0.0	0.0	2.538776058323998	0.0
7	3.5339310388697077E-4	0.0	0.0	3.1116262797247773	0.0
8	3.5339310388697077E-4	0.0	0.0	4.298673715681113	0.0
9	3.5339310388697077E-4	0.0	0.0	4.754904212799191	0.0
10	3.5339310388697077E-4	0.0	0.0	5.787165469253033	0.0
11	3.5339310388697077E-4	0.0	0.0	6.966438256923855	0.0
12	3.5339310388697077E-4	0.0	0.0	8.021316672026462	0.0
13	3.5339310388697077E-4	0.0	0.0	8.563775086492962	0.0
14	3.5339310388697077E-4	0.0	0.0	8.820338479914904	0.0
15	3.5339310388697077E-4	0.0	0.0	9.158182287230847	0.0
16	3.5339310388697077E-4	0.0	0.0	9.837757226005492	0.0
17	3.5339310388697077E-4	0.0	0.0	10.633245102855064	0.0
18	3.5339310388697077E-4	0.0	0.0	11.69978549038594	0.0
19	3.5339310388697077E-4	0.0	0.0	12.163437242685646	0.0
20	3.5339310388697077E-4	0.0	0.0	12.65536044329631	0.0
21	3.5339310388697077E-4	0.0	0.0	13.234571740567054	0.0
22	3.5339310388697077E-4	0.0	0.0	13.844528237875966	0.0
23	3.5339310388697077E-4	0.0	0.0	14.470740817963678	0.0
24	3.5339310388697077E-4	0.0	0.0	14.937573108198366	0.0
25	3.5339310388697077E-4	0.0	0.0	15.334786956967323	0.0
26	3.5339310388697077E-4	0.0	0.0	15.71539132985359	0.0
27	3.5339310388697077E-4	0.0	0.0	16.095288916532084	0.0
28	3.5339310388697077E-4	0.0	0.0	16.511232599807048	0.0
29	3.5339310388697077E-4	0.0	0.0	16.894310724420524	0.0
30	3.5339310388697077E-4	0.0	0.0	17.35301497326581	0.0
31	3.5339310388697077E-4	0.0	0.0	17.768251870333003	0.0
32	3.5339310388697077E-4	0.0	0.0	18.176420905322452	0.0
33	3.5339310388697077E-4	0.0	0.0	18.587063692039113	0.0
34	3.5339310388697077E-4	0.0	0.0	19.00053362358687	0.0
35	3.5339310388697077E-4	0.0	0.0	19.423898562043462	0.0
36	3.5339310388697077E-4	0.0	0.0	19.811924190111355	0.0
37	3.5339310388697077E-4	0.0	0.0	20.243063776853457	0.0
38	3.5339310388697077E-4	0.0	0.0	20.649465846323476	0.0
39	3.5339310388697077E-4	0.0	0.0	21.054454343377945	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAATC	20	7.0242654E-4	45.0	8
AGTCATA	25	3.88333E-5	45.0	25
CGTATGG	25	3.88333E-5	45.0	2
TTAGCCG	20	7.0242654E-4	45.0	1
TAGACGT	20	7.0242654E-4	45.0	30
CGTTGGC	20	7.0242654E-4	45.0	25
ATTCGTT	20	7.0242654E-4	45.0	22
CTAAGGC	20	7.0242654E-4	45.0	4
ACGGGCC	20	7.0242654E-4	45.0	5
GCATTAT	20	7.0242654E-4	45.0	9
TTAAGCG	20	7.0242654E-4	45.0	1
TACTCTA	25	3.88333E-5	45.0	29
ACCAAGC	20	7.0242654E-4	45.0	17
GATTCGT	20	7.0242654E-4	45.0	21
GGTTACG	20	7.0242654E-4	45.0	1
ACGAACA	25	3.88333E-5	45.0	29
CGGACGG	25	3.88333E-5	45.0	2
CCTCCGC	80	0.0	42.1875	45
TTACGGG	70	0.0	41.785717	3
GTCATAC	55	6.002665E-11	40.90909	39
>>END_MODULE