FastQCFastQC Report
Sat 14 Jan 2017
SRR2936129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956738
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121491.269835628980975No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35330.3692756010527438No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24150.25242020281414557No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.16420378410808395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC453.8380676E-1045.00000412
TCTAACG351.211647E-745.0000041
TTTAGCG253.8903137E-545.01
GATACGT207.032693E-445.09
CGCATAG302.1651194E-644.9999962
CGTTTTT43150.043.1228261
CGTTATT10350.042.8260881
CGGTTTA750.042.01
TTGTACG700.041.7857171
CGGTTTT11100.041.5540541
CGTTTTA4750.041.2105261
CCACGTC556.002665E-1140.90909233
ACGGGCT2550.039.7058835
TCGACGG659.094947E-1238.0769272
GCTACGA1200.037.49999610
TACGGGA4100.037.3170744
CTAACGG850.037.0588232
CGAATAT1250.036.00000414
TCACGAC504.8774382E-836.025
CGCATCG504.8774382E-836.021
Produced by FastQC (version 0.11.5)