##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936128.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367154
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.791926548532768	31.0	31.0	34.0	30.0	34.0
2	31.905211437162606	31.0	31.0	34.0	30.0	34.0
3	31.837920872440446	33.0	31.0	34.0	30.0	34.0
4	35.59082564809317	37.0	35.0	37.0	33.0	37.0
5	31.1451080473044	37.0	35.0	37.0	0.0	37.0
6	33.32141826045746	37.0	35.0	37.0	17.0	37.0
7	29.439788208762536	35.0	32.0	37.0	0.0	37.0
8	32.4174760454741	35.0	33.0	37.0	17.0	37.0
9	36.269181324457854	39.0	35.0	39.0	32.0	39.0
10	36.56494549970857	38.0	35.0	39.0	32.0	39.0
11	36.74908893815674	38.0	35.0	39.0	33.0	39.0
12	36.737257390631726	39.0	35.0	39.0	33.0	39.0
13	36.52663187654227	39.0	35.0	39.0	32.0	39.0
14	37.78686055442675	40.0	36.0	41.0	33.0	41.0
15	37.845318313296325	40.0	36.0	41.0	33.0	41.0
16	37.93570817695027	40.0	36.0	41.0	33.0	41.0
17	37.8503216633892	40.0	36.0	41.0	33.0	41.0
18	37.73488508909068	39.0	36.0	41.0	33.0	41.0
19	37.64305986044003	39.0	36.0	41.0	33.0	41.0
20	37.60196266416817	39.0	36.0	41.0	33.0	41.0
21	37.53884745910435	39.0	36.0	41.0	33.0	41.0
22	37.5165516377324	39.0	36.0	41.0	33.0	41.0
23	37.50472826116562	39.0	36.0	41.0	33.0	41.0
24	37.469762007223125	39.0	35.0	41.0	33.0	41.0
25	37.354341774841075	39.0	35.0	41.0	32.0	41.0
26	37.18984676729656	39.0	35.0	41.0	32.0	41.0
27	37.10417699384999	39.0	35.0	41.0	32.0	41.0
28	36.894014500727216	39.0	35.0	41.0	31.0	41.0
29	36.812223208789774	39.0	35.0	41.0	31.0	41.0
30	36.62214493100988	39.0	35.0	40.0	30.0	41.0
31	36.545958916421995	39.0	35.0	40.0	30.0	41.0
32	36.457470162384176	39.0	35.0	40.0	30.0	41.0
33	36.23846941610332	39.0	35.0	40.0	30.0	41.0
34	36.18057817700475	39.0	35.0	40.0	30.0	41.0
35	36.01882861142736	39.0	35.0	40.0	29.0	41.0
36	35.849569935231536	38.0	35.0	40.0	28.0	41.0
37	35.65572484570507	38.0	35.0	40.0	27.0	41.0
38	35.55925578912391	38.0	35.0	40.0	27.0	41.0
39	35.54744058351537	38.0	35.0	40.0	27.0	41.0
40	35.40917435190683	38.0	34.0	40.0	26.0	41.0
41	35.303499349046994	38.0	34.0	40.0	25.0	41.0
42	35.270368837054754	38.0	34.0	40.0	25.0	41.0
43	35.02443661242966	38.0	34.0	40.0	24.0	41.0
44	34.87616640428812	38.0	34.0	40.0	24.0	41.0
45	34.81762965948894	38.0	34.0	40.0	23.0	41.0
46	34.61945396209765	38.0	34.0	40.0	23.0	41.0
47	34.41773206883215	38.0	33.0	40.0	23.0	41.0
48	34.3399200335554	37.0	33.0	40.0	23.0	41.0
49	34.14587067007305	37.0	33.0	40.0	22.0	41.0
50	33.964129493346114	37.0	33.0	40.0	20.0	41.0
51	31.796071948010916	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	19.0
10	20.0
11	18.0
12	10.0
13	8.0
14	8.0
15	29.0
16	50.0
17	90.0
18	221.0
19	440.0
20	828.0
21	1300.0
22	1831.0
23	2491.0
24	3010.0
25	3901.0
26	4547.0
27	5410.0
28	6253.0
29	7448.0
30	8739.0
31	10909.0
32	14170.0
33	19580.0
34	27659.0
35	33532.0
36	36021.0
37	53874.0
38	64868.0
39	59856.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.723195171508415	19.917527794876264	23.572942144168387	11.786334889446936
2	29.438055965616606	28.246185524330393	25.288298643076203	17.0274598669768
3	31.070068690522234	27.20139233128333	25.536151042886633	16.1923879353078
4	27.821568061358448	26.29931854208316	29.06437080897934	16.814742587579055
5	23.601812863267185	38.44217957587279	21.2959684492066	16.660039111653422
6	24.835627556829014	37.048214100894995	24.710067165276694	13.406091176999297
7	67.30091460259183	19.803679110128176	9.082564809317072	3.812841477962926
8	80.4063145165244	7.851746133774928	7.768674725047256	3.973264624653415
9	75.27604220572293	7.4554546593527515	9.70274053939219	7.56576259553212
10	40.54729078261438	25.12379001726796	19.415558593941505	14.913360606176154
11	31.77821840426633	27.690015633766755	23.376839146516176	17.15492681545074
12	26.166404288118883	25.09954950783595	28.99600712507558	19.73803907896959
13	24.0661956563186	26.97042657849295	31.1594045005638	17.803973264624652
14	20.977301078021757	30.623389640314418	28.3363384301955	20.06297085146832
15	19.27202209427107	26.971243674316497	36.09575273590918	17.66098149550325
16	21.45475740424999	27.136569395948296	30.212390441068326	21.196282758733393
17	22.37480730156828	25.681321734204175	28.422678222217378	23.521192742010165
18	23.72301541042723	27.25368646399059	28.74869945581418	20.274598669768
19	24.375057877620833	28.594268345163066	26.36632039961433	20.664353377601767
20	24.48563817907472	27.724878388904926	28.413417802883806	19.376065629136548
21	23.83250625078305	29.945744837316223	27.39232038871972	18.829428523181008
22	21.36596632475746	26.880001307353318	27.439984311760192	24.314048056129035
23	20.71065547426965	29.081802186548423	28.133971031229404	22.073571307952523
24	21.429427433719912	27.104157928280774	28.73971140175512	22.72670323624419
25	20.47424241598893	30.44036017583902	26.72148471758445	22.3639126905876
26	20.61478289763968	29.067639192273543	27.11886565310469	23.198712256982084
27	21.94828328167472	29.27164078288674	26.63514492556257	22.144931009875965
28	19.868229680188694	28.558316128926826	30.31561688011025	21.257837310774224
29	22.729426888989362	26.26826890078823	28.14323145056298	22.859072759659433
30	21.64159998256862	29.50968803281457	28.852198260130624	19.996513724486185
31	25.52144331806272	28.83585634365961	24.12066871122199	21.522031627055675
32	23.331354145671842	29.389030216203555	25.301916906802052	21.97769873132255
33	22.004390528225212	27.817754947515212	26.999297297591745	23.178557226667827
34	23.306296540416284	26.660202530818133	26.778953790507526	23.25454713825806
35	20.799718919036696	27.564455242214436	26.80973106652794	24.826094772220923
36	23.47407354951873	29.415994378380734	26.328733991730992	20.781198080369546
37	21.06827107971042	30.02554786274969	26.94945445235514	21.956726605184745
38	21.422345936582467	28.308829537469293	26.456200940204926	23.81262358574331
39	22.48239158500248	26.141346682863322	26.90669310425598	24.469568627878218
40	22.033261247324013	25.825675329698168	29.009897754075947	23.131165668901875
41	19.615202340162437	28.319451783175452	25.69058215353775	26.374763723124357
42	21.577866508331653	26.86801723527457	27.616749374921696	23.93736688147208
43	22.76728566214722	26.76669735315426	26.602461092620533	23.863555892077983
44	20.996366647237945	26.545264384971972	27.13411810847764	25.324250859312443
45	21.033135959297734	26.252471714866243	26.195547372492197	26.51884495334383
46	23.00533291207504	28.096656988620577	25.38989089047103	23.50811920883335
47	20.598440981168665	25.906567816229703	29.67256246697571	23.822428735625923
48	21.331375934893803	26.243756026081698	27.880398960654112	24.544469078370383
49	20.962593353197843	24.64606132576521	30.058231695691727	24.333113625345224
50	20.583188525795716	24.737576058002908	30.32133655087511	24.357898865326266
51	20.902945358078625	25.23028483960409	26.832882114861885	27.033887687455397
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	389.0
1	546.5
2	704.0
3	1108.0
4	1512.0
5	1225.5
6	939.0
7	846.0
8	753.0
9	782.5
10	812.0
11	800.0
12	788.0
13	805.5
14	823.0
15	871.5
16	920.0
17	886.0
18	852.0
19	904.5
20	957.0
21	1203.5
22	1450.0
23	1660.0
24	1870.0
25	2348.0
26	3343.5
27	3861.0
28	4557.0
29	5253.0
30	6393.0
31	7533.0
32	8080.5
33	8628.0
34	9797.0
35	10966.0
36	11783.0
37	12600.0
38	13718.5
39	14837.0
40	17005.5
41	19174.0
42	20863.5
43	22553.0
44	24115.5
45	25678.0
46	28250.5
47	30823.0
48	35470.0
49	40117.0
50	36246.0
51	32375.0
52	30589.0
53	28803.0
54	25462.0
55	22121.0
56	19358.0
57	16595.0
58	15067.0
59	13539.0
60	12268.5
61	10998.0
62	9693.5
63	8389.0
64	6968.0
65	5547.0
66	4618.5
67	3690.0
68	3141.0
69	2592.0
70	2220.5
71	1849.0
72	1481.5
73	1114.0
74	912.0
75	583.0
76	456.0
77	408.0
78	360.0
79	267.5
80	175.0
81	140.5
82	106.0
83	74.5
84	43.0
85	41.5
86	40.0
87	24.5
88	9.0
89	11.0
90	13.0
91	10.5
92	8.0
93	5.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	367154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.52439096307826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.83058505712079	29.576332997935246
2	8.806613282947605	6.961520529117187
3	3.8774091011281437	4.597566997103598
4	2.4316710289650554	3.8444126576962265
5	1.7181028603382082	3.395348458339519
6	1.1827896110679612	2.8049423408950425
7	0.8671668288625936	2.399196854192455
8	0.691061952199562	2.1851042222754873
9	0.5831661374830728	2.0744357772878295
>10	4.938985550731391	36.90514176852357
>50	0.04415914005675642	1.149757952794491
>100	0.024149529718538666	1.9209902797638505
>500	0.002069959690160457	0.5009737569457969
>1k	0.002069959690160457	1.6842754071296908
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	2152	0.5861300707604983	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGC	2067	0.5629790224265567	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG	1957	0.5330188422296911	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	710	0.19337934490704173	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	587	0.15987841614145562	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.1470772482391585	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT	418	0.11384868474808936	No Hit
GAATATGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	398	0.10840137925775015	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGC	390	0.10622245706161447	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGC	383	0.10431590013999575	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG	378	0.10295407376741095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7236527451696016E-4	0.0	0.0	0.10486063068902968	0.0
2	2.7236527451696016E-4	0.0	0.0	0.5504502197987765	0.0
3	2.7236527451696016E-4	0.0	0.0	0.7133246539599187	0.0
4	2.7236527451696016E-4	0.0	0.0	1.0516023249099833	0.0
5	2.7236527451696016E-4	0.0	0.0	2.496772471496974	0.0
6	2.7236527451696016E-4	0.0	0.0	3.2046498199665536	0.0
7	2.7236527451696016E-4	0.0	0.0	3.762181536902771	0.0
8	2.7236527451696016E-4	0.0	0.0	4.671881553789418	0.0
9	2.7236527451696016E-4	0.0	0.0	4.931718025678598	0.0
10	2.7236527451696016E-4	0.0	0.0	6.544120450819002	0.0
11	2.7236527451696016E-4	0.0	0.0	7.473158402196353	0.0
12	2.7236527451696016E-4	0.0	0.0	9.191783284398372	0.0
13	2.7236527451696016E-4	0.0	0.0	9.679317125783731	0.0
14	2.7236527451696016E-4	0.0	0.0	9.919815663182208	0.0
15	2.7236527451696016E-4	0.0	0.0	10.257548603583238	0.0
16	2.7236527451696016E-4	0.0	0.0	10.82624729677465	0.0
17	2.7236527451696016E-4	0.0	0.0	11.474476650125016	0.0
18	2.7236527451696016E-4	0.0	0.0	12.232741574380233	0.0
19	2.7236527451696016E-4	0.0	0.0	12.633390893194681	0.0
20	2.7236527451696016E-4	0.0	0.0	13.040576978597537	0.0
21	2.7236527451696016E-4	0.0	0.0	13.546631658650048	0.0
22	2.7236527451696016E-4	0.0	0.0	14.064942776055824	0.0
23	5.447305490339203E-4	0.0	0.0	14.594420869716794	0.0
24	5.447305490339203E-4	0.0	0.0	14.989350517766386	0.0
25	5.447305490339203E-4	0.0	0.0	15.35377525507008	0.0
26	5.447305490339203E-4	0.0	0.0	15.725009124236696	0.0
27	5.447305490339203E-4	0.0	0.0	16.079901076932295	0.0
28	5.447305490339203E-4	0.0	0.0	16.44759419753019	0.0
29	5.447305490339203E-4	0.0	0.0	16.821006988892943	0.0
30	5.447305490339203E-4	0.0	0.0	17.25406777537491	0.0
31	5.447305490339203E-4	0.0	0.0	17.641098830463513	0.0
32	5.447305490339203E-4	0.0	0.0	18.009336681610442	0.0
33	5.447305490339203E-4	0.0	0.0	18.405083425483586	0.0
34	5.447305490339203E-4	0.0	0.0	18.78258169596409	0.0
35	5.447305490339203E-4	0.0	0.0	19.180234996758852	0.0
36	5.447305490339203E-4	0.0	0.0	19.55146886592547	0.0
37	5.447305490339203E-4	0.0	0.0	19.905816088072037	0.0
38	5.447305490339203E-4	0.0	0.0	20.281135436356408	0.0
39	5.447305490339203E-4	0.0	0.0	20.691317539778947	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	70	0.0	45.000004	1
CGAGCAC	35	1.2089004E-7	45.000004	25
TCGCCAC	25	3.8856022E-5	45.0	43
CGTATCG	20	7.0270087E-4	45.0	28
CCGATTC	20	7.0270087E-4	45.0	10
ACCGAAG	25	3.8856022E-5	45.0	32
TGACCGA	25	3.8856022E-5	45.0	30
TCTCGAA	20	7.0270087E-4	45.0	32
TCGTAAG	20	7.0270087E-4	45.0	1
CTCGAAA	20	7.0270087E-4	45.0	33
TTACGAG	20	7.0270087E-4	45.0	25
AACTCGT	20	7.0270087E-4	45.0	7
CGTCATA	25	3.8856022E-5	45.0	38
TCGCGTT	25	3.8856022E-5	45.0	36
TACACGA	20	7.0270087E-4	45.0	26
TACGTGG	20	7.0270087E-4	45.0	2
CGCTTAG	20	7.0270087E-4	45.0	2
TGCGTAG	20	7.0270087E-4	45.0	1
TGGTACG	20	7.0270087E-4	45.0	1
TATGCGG	30	2.1614524E-6	44.999996	2
>>END_MODULE