##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936127.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	588036
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76367943459244	31.0	31.0	34.0	30.0	34.0
2	31.887829996802918	33.0	31.0	34.0	30.0	34.0
3	31.68710249032372	33.0	31.0	34.0	30.0	34.0
4	35.509902454951735	37.0	35.0	37.0	33.0	37.0
5	31.0115248046038	37.0	35.0	37.0	0.0	37.0
6	33.29155357835234	37.0	35.0	37.0	17.0	37.0
7	29.666938758851497	35.0	32.0	37.0	0.0	37.0
8	32.67922202042052	35.0	33.0	37.0	17.0	37.0
9	36.480426368453635	39.0	35.0	39.0	32.0	39.0
10	36.686612724391026	38.0	35.0	39.0	32.0	39.0
11	36.86106122754389	39.0	37.0	39.0	33.0	39.0
12	36.92449441870906	39.0	37.0	39.0	33.0	39.0
13	36.78961662211157	39.0	37.0	39.0	33.0	39.0
14	38.03433293199736	40.0	37.0	41.0	33.0	41.0
15	38.1060955451707	40.0	37.0	41.0	33.0	41.0
16	38.14126686121258	40.0	37.0	41.0	33.0	41.0
17	38.11416818017945	40.0	37.0	41.0	33.0	41.0
18	38.00897053921869	40.0	37.0	41.0	33.0	41.0
19	37.8710521124557	40.0	37.0	41.0	33.0	41.0
20	37.773699229298884	39.0	36.0	41.0	33.0	41.0
21	37.69578733274834	39.0	36.0	41.0	33.0	41.0
22	37.68419790625064	39.0	36.0	41.0	33.0	41.0
23	37.628481929677775	39.0	36.0	41.0	33.0	41.0
24	37.58371256181594	39.0	36.0	41.0	33.0	41.0
25	37.4675513063826	39.0	35.0	41.0	33.0	41.0
26	37.3083110557857	39.0	35.0	41.0	32.0	41.0
27	37.27615826241931	39.0	35.0	41.0	32.0	41.0
28	37.160359229707026	39.0	35.0	41.0	32.0	41.0
29	37.144904393608556	39.0	35.0	41.0	32.0	41.0
30	36.99362624057031	39.0	35.0	41.0	31.0	41.0
31	36.8757099905448	39.0	35.0	41.0	31.0	41.0
32	36.74496629458061	39.0	35.0	41.0	31.0	41.0
33	36.57232210272841	39.0	35.0	40.0	30.0	41.0
34	36.392528688719736	39.0	35.0	40.0	30.0	41.0
35	36.21962770986811	39.0	35.0	40.0	30.0	41.0
36	36.090383922072796	39.0	35.0	40.0	29.0	41.0
37	35.97713405301716	39.0	35.0	40.0	29.0	41.0
38	35.90117611846894	39.0	35.0	40.0	28.0	41.0
39	35.88500874096144	39.0	35.0	40.0	28.0	41.0
40	35.76501268629812	39.0	35.0	40.0	27.0	41.0
41	35.698569475338246	39.0	35.0	40.0	27.0	41.0
42	35.64689066655783	38.0	35.0	40.0	27.0	41.0
43	35.47534674747804	38.0	35.0	40.0	26.0	41.0
44	35.348789870007955	38.0	34.0	40.0	26.0	41.0
45	35.197703882075245	38.0	34.0	40.0	25.0	41.0
46	35.06321211626499	38.0	34.0	40.0	24.0	41.0
47	34.907223367276835	38.0	34.0	40.0	24.0	41.0
48	34.81244685699515	38.0	34.0	40.0	23.0	41.0
49	34.675072614601824	38.0	34.0	40.0	23.0	41.0
50	34.46014699780286	38.0	33.0	40.0	23.0	41.0
51	32.50251855328585	36.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	24.0
10	30.0
11	23.0
12	21.0
13	18.0
14	27.0
15	47.0
16	68.0
17	135.0
18	251.0
19	494.0
20	821.0
21	1362.0
22	2033.0
23	3085.0
24	4502.0
25	6117.0
26	7663.0
27	8600.0
28	9165.0
29	10613.0
30	13056.0
31	16224.0
32	20900.0
33	28804.0
34	40685.0
35	50123.0
36	58291.0
37	90220.0
38	111130.0
39	103482.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84759436497085	20.84481222238094	21.917025488235414	12.390567924412792
2	34.640396166221116	24.0170669822936	24.291369916127582	17.0511669353577
3	31.911141494738416	24.481494330279098	27.28982579297866	16.317538382003825
4	27.208028079913475	25.62751260126931	28.04522172111912	19.1192375976981
5	21.066397295403682	39.61203055595235	23.23939350652001	16.08217864212395
6	24.550877837411313	35.8301192444	26.09483092871865	13.524171989470032
7	68.29241747103919	19.45340081219517	8.439619343033419	3.8145623737322207
8	82.21367399274875	5.086933453053895	8.949282016747274	3.7501105374500883
9	76.59786135542723	7.469270588875511	10.885217911828528	5.047650143868743
10	38.05549320109653	26.243971457529813	20.53037569128421	15.17015965008945
11	29.335788965301447	28.30200872055452	24.710221823153685	17.651980490990347
12	26.30349162296186	25.36494364290622	29.71127618036991	18.620288553762016
13	23.653143685080504	26.729145834608765	31.02837241257338	18.589338067737348
14	20.985449870416097	29.409253855206142	30.19474998129366	19.410546293084096
15	20.127509200117	27.838771775877667	33.67004741206321	18.363671611942127
16	22.61341142379038	26.57439340448544	31.746525722914924	19.065669448809256
17	22.925127033038795	26.73118652599501	29.214537885435586	21.129148555530612
18	24.02029807698848	26.757885571631668	29.867559129032916	19.35425722234693
19	24.1884850587379	29.35687610962594	27.5358991626363	18.918739668999855
20	24.40734920991232	28.305069757633888	28.97577699324531	18.311804039208486
21	23.406049969729743	28.485500887700756	29.31725268520975	18.791196457359753
22	22.360365691896416	26.5715024250216	28.634131243665355	22.434000639416634
23	21.073709772871048	28.501316245944125	29.28358127733676	21.141392703848062
24	21.78999244944187	26.92862341761389	30.455448305886033	20.825935827058206
25	21.507356692447402	28.511859818106373	28.550462896829448	21.430320592616777
26	20.784441768871293	29.509417790747506	29.13257011475488	20.57357032562632
27	21.599017747212756	29.165051119319223	28.66967328530906	20.566257848158955
28	19.620907563482508	29.17355400009523	30.16226897672932	21.043269459692944
29	21.920256582930296	28.268337312681535	28.814222258501182	20.997183845886987
30	21.14955546939303	28.450639076519124	29.12355706113231	21.276248392955534
31	21.985218592058988	28.69144066009564	27.568210109585127	21.75513063826024
32	21.76856518988633	29.765014386874274	27.664632777585048	20.80178764565435
33	21.202273330204275	30.050541123332586	26.907536273289388	21.83964927317375
34	20.609962655347633	29.235965144991123	28.242148439891434	21.91192375976981
35	20.589725799100737	29.875211721731322	27.522294553394687	22.012767925773254
36	20.733934657061813	31.344679577440836	26.617077865980992	21.304307899516356
37	19.6850192845336	32.22846900529899	27.449169778721032	20.63734193144637
38	19.59675938207865	31.22342849757498	26.82862953968805	22.351182580658328
39	20.951098232081026	29.817052017223432	26.663673652633513	22.568176098062022
40	21.26060309232768	28.726642586508312	28.600289778176847	21.412464542987163
41	19.05682645280221	29.951907706330903	28.18636954200083	22.804896298866055
42	20.380554932010966	29.563836227713953	27.825847397098137	22.229761443176947
43	20.50996877742179	29.5009149099715	27.52246461101021	22.4666517015965
44	20.13958329081893	28.90095164241645	27.567699936738567	23.39176513002605
45	20.761994163622635	28.64076349067064	27.147317511172787	23.449924834533938
46	21.083743172186736	30.151385289336023	26.58612737995633	22.178744158520907
47	20.29110462624737	29.986429402281495	27.89080940622683	21.8316565652443
48	20.005067716942502	29.22559163044439	27.86734145528505	22.901999197328056
49	20.402152249182024	28.185009081076668	28.303709296709723	23.109129373031585
50	19.07043106204382	28.252692012053682	28.682937779319634	23.993939146582864
51	19.578563217217994	27.494915277295952	26.953451829479828	25.973069676006233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	757.0
1	1023.0
2	1289.0
3	3437.0
4	5585.0
5	4531.0
6	3477.0
7	2793.0
8	2109.0
9	2096.0
10	2083.0
11	2055.5
12	2028.0
13	1996.0
14	1964.0
15	1917.5
16	1871.0
17	1922.0
18	1973.0
19	2037.0
20	2101.0
21	2279.5
22	2458.0
23	2700.0
24	2942.0
25	3721.0
26	5303.5
27	6107.0
28	7525.0
29	8943.0
30	10311.5
31	11680.0
32	13711.5
33	15743.0
34	17798.0
35	19853.0
36	21040.0
37	22227.0
38	24451.5
39	26676.0
40	30175.5
41	33675.0
42	37736.0
43	41797.0
44	47408.5
45	53020.0
46	54369.5
47	55719.0
48	53868.0
49	52017.0
50	47893.5
51	43770.0
52	39422.0
53	35074.0
54	31939.0
55	28804.0
56	26598.5
57	24393.0
58	22066.0
59	19739.0
60	18565.0
61	17391.0
62	15477.0
63	13563.0
64	10667.5
65	7772.0
66	6393.0
67	5014.0
68	4200.5
69	3387.0
70	2848.0
71	2309.0
72	2011.5
73	1714.0
74	1379.5
75	815.5
76	586.0
77	462.5
78	339.0
79	259.5
80	180.0
81	151.0
82	122.0
83	112.0
84	102.0
85	77.5
86	53.0
87	48.5
88	44.0
89	32.5
90	21.0
91	17.5
92	14.0
93	9.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	588036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.26522001357227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00973787930228	32.88578032521971
2	8.876773211267903	7.681110919921805
3	3.7415350073847664	4.856350058489539
4	2.260536269894737	3.912103962626231
5	1.5549961439634845	3.3638625144418324
6	1.1065636866768604	2.8725432817862413
7	0.8508108668129462	2.5767363539820174
8	0.683733942772783	2.3665519571849347
9	0.5528655338491861	2.152786406391551
>10	4.29611614002188	33.94382741840957
>50	0.05005369400419686	1.4722450489974424
>100	0.01546374284649407	1.3586839313076784
>500	4.0694060122352816E-4	0.14578078205556014
>1k	4.0694060122352816E-4	0.4116370391858691
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2338	0.3975947050860832	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	828	0.14080770565067446	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.401152310402764E-4	0.0	0.0	0.08740961437735105	0.0
2	3.401152310402764E-4	0.0	0.0	0.38347992299791167	0.0
3	3.401152310402764E-4	0.0	0.0	0.5433340815868416	0.0
4	3.401152310402764E-4	0.0	0.0	0.744342183131645	0.0
5	3.401152310402764E-4	0.0	0.0	1.401274751885939	0.0
6	3.401152310402764E-4	0.0	0.0	1.9238618043793236	0.0
7	3.401152310402764E-4	0.0	0.0	2.3797862715888143	0.0
8	3.401152310402764E-4	0.0	0.0	3.2770102510730634	0.0
9	3.401152310402764E-4	0.0	0.0	3.6492663714466462	0.0
10	3.401152310402764E-4	0.0	0.0	4.576080376031399	0.0
11	3.401152310402764E-4	0.0	0.0	5.578570019522615	0.0
12	3.401152310402764E-4	0.0	0.0	6.382772483317348	0.0
13	3.401152310402764E-4	0.0	0.0	6.7276493275921885	0.0
14	3.401152310402764E-4	0.0	0.0	6.869987551782544	0.0
15	3.401152310402764E-4	0.0	0.0	7.125584147909311	0.0
16	3.401152310402764E-4	0.0	0.0	7.668918229496153	0.0
17	3.401152310402764E-4	0.0	0.0	8.345747539266304	0.0
18	3.401152310402764E-4	0.0	0.0	9.063050561530247	0.0
19	3.401152310402764E-4	0.0	0.0	9.499418402954921	0.0
20	3.401152310402764E-4	0.0	0.0	9.911468005360216	0.0
21	3.401152310402764E-4	0.0	0.0	10.453271568407377	0.0
22	3.401152310402764E-4	0.0	0.0	11.064968811433314	0.0
23	3.401152310402764E-4	0.0	0.0	11.707616540483915	0.0
24	3.401152310402764E-4	0.0	0.0	12.185818555326545	0.0
25	3.401152310402764E-4	0.0	0.0	12.627458182832344	0.0
26	3.401152310402764E-4	0.0	0.0	13.067567291798461	0.0
27	3.401152310402764E-4	0.0	0.0	13.482847988898639	0.0
28	3.401152310402764E-4	0.0	0.0	13.945404703113415	0.0
29	3.401152310402764E-4	0.0	0.0	14.403029746478106	0.0
30	3.401152310402764E-4	0.0	0.0	14.93565019828718	0.0
31	3.401152310402764E-4	0.0	0.0	15.447693678618316	0.0
32	3.401152310402764E-4	0.0	0.0	15.937459611316314	0.0
33	3.401152310402764E-4	0.0	0.0	16.431987157248876	0.0
34	3.401152310402764E-4	0.0	0.0	16.88332006883932	0.0
35	3.401152310402764E-4	0.0	0.0	17.380398479004686	0.0
36	3.401152310402764E-4	0.0	0.0	17.858090321000756	0.0
37	3.401152310402764E-4	0.0	0.0	18.337482739152026	0.0
38	3.401152310402764E-4	0.0	0.0	18.827928902312102	0.0
39	3.401152310402764E-4	0.0	0.0	19.3142936826997	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	60	0.0	45.000004	9
TTCACCG	20	7.0304726E-4	45.0	38
TAGCCCG	25	3.888474E-5	45.0	27
ACCGTTT	20	7.0304726E-4	45.0	41
TCGACGT	40	6.8048394E-9	45.0	26
CGTTATT	190	0.0	41.447372	1
AGTACGG	60	3.6379788E-12	41.250004	2
CTGACGG	55	6.002665E-11	40.909092	2
ATTACGG	50	1.0804797E-9	40.5	2
TTTCGAC	45	1.9255822E-8	40.0	24
AGGCGTC	45	1.9255822E-8	40.0	35
TACGGGA	180	0.0	40.0	4
AATTGCG	45	1.9255822E-8	40.0	1
CGGTTTT	370	0.0	39.52703	1
GCGTCAT	40	3.4543882E-7	39.375	37
CTTACGG	40	3.4543882E-7	39.375	2
CACGGGA	230	0.0	39.130432	4
CGCACGG	75	0.0	39.0	2
CGTTTTT	1255	0.0	38.904385	1
ACGGGAA	175	0.0	38.571426	5
>>END_MODULE