##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936126.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	224839
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.605842402785992	31.0	31.0	34.0	30.0	34.0
2	31.72199218107179	31.0	31.0	34.0	30.0	34.0
3	31.686211022109152	31.0	31.0	34.0	30.0	34.0
4	35.44647948087298	37.0	35.0	37.0	33.0	37.0
5	30.947197772628414	35.0	35.0	37.0	0.0	37.0
6	33.18085385542544	36.0	35.0	37.0	17.0	37.0
7	29.63375570964112	35.0	32.0	37.0	0.0	37.0
8	32.57941015571142	35.0	33.0	37.0	17.0	37.0
9	36.258931946859754	39.0	35.0	39.0	32.0	39.0
10	36.54156974546231	38.0	35.0	39.0	32.0	39.0
11	36.72817438255819	38.0	35.0	39.0	33.0	39.0
12	36.68830585441138	39.0	35.0	39.0	33.0	39.0
13	36.35704659778775	39.0	35.0	39.0	32.0	39.0
14	37.45817673980048	39.0	36.0	41.0	32.0	41.0
15	37.57094187396315	39.0	36.0	41.0	33.0	41.0
16	37.697734823584874	39.0	36.0	41.0	33.0	41.0
17	37.66226944613701	39.0	36.0	41.0	33.0	41.0
18	37.552115069004934	39.0	36.0	41.0	33.0	41.0
19	37.49493637669622	39.0	36.0	41.0	32.0	41.0
20	37.43960789720644	39.0	36.0	41.0	32.0	41.0
21	37.34196914236409	39.0	35.0	40.0	32.0	41.0
22	37.30346603569665	39.0	35.0	40.0	32.0	41.0
23	37.275521595452744	39.0	35.0	40.0	32.0	41.0
24	37.240238570710595	39.0	35.0	40.0	32.0	41.0
25	37.12664617793177	39.0	35.0	40.0	32.0	41.0
26	36.92181516551844	39.0	35.0	40.0	31.0	41.0
27	36.93625216265861	39.0	35.0	40.0	31.0	41.0
28	36.721547418374925	39.0	35.0	40.0	31.0	41.0
29	36.60475273417868	39.0	35.0	40.0	31.0	41.0
30	36.52323217947065	39.0	35.0	40.0	30.0	41.0
31	36.41358038418602	38.0	35.0	40.0	30.0	41.0
32	36.297964321136455	38.0	35.0	40.0	30.0	41.0
33	36.21638149965086	38.0	35.0	40.0	30.0	41.0
34	36.11704375130649	38.0	35.0	40.0	30.0	41.0
35	36.061319433016514	38.0	35.0	40.0	30.0	41.0
36	35.98638581384902	38.0	35.0	40.0	29.0	41.0
37	35.874132156787745	38.0	35.0	40.0	29.0	41.0
38	35.74261137969836	38.0	35.0	40.0	28.0	41.0
39	35.785157379280285	38.0	35.0	40.0	29.0	41.0
40	35.6587691637127	38.0	35.0	40.0	28.0	41.0
41	35.53279012982623	38.0	35.0	40.0	27.0	41.0
42	35.52736847255147	38.0	35.0	40.0	27.0	41.0
43	35.25453769141475	38.0	34.0	40.0	26.0	41.0
44	34.95005314914228	38.0	34.0	40.0	24.0	41.0
45	34.846974946517285	38.0	34.0	40.0	24.0	41.0
46	34.64649816090625	38.0	33.0	40.0	23.0	41.0
47	34.509444535867885	38.0	33.0	40.0	23.0	41.0
48	34.326469162378416	37.0	33.0	40.0	23.0	41.0
49	34.051143262512284	37.0	33.0	40.0	22.0	41.0
50	33.801293369922476	37.0	33.0	40.0	20.0	41.0
51	31.395060465488637	35.0	28.0	39.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	8.0
11	10.0
12	4.0
13	5.0
14	9.0
15	11.0
16	19.0
17	37.0
18	135.0
19	258.0
20	456.0
21	706.0
22	947.0
23	1308.0
24	1690.0
25	2241.0
26	2576.0
27	3193.0
28	3811.0
29	4734.0
30	5588.0
31	7316.0
32	9488.0
33	13019.0
34	18240.0
35	22080.0
36	22842.0
37	32788.0
38	38737.0
39	32571.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.85901467272137	20.490217444482496	26.445145192782395	12.205622690013742
2	27.869720110834862	27.07226059535935	27.586851035629937	17.47116825817585
3	29.641654695137408	27.02333669870441	27.488558479623197	15.846450126534986
4	27.588630086417393	25.856724144832526	28.584898527390713	17.969747241359375
5	21.96238197109932	38.28117008170291	23.023141003117786	16.733306944079988
6	23.802365248021918	34.624331188094594	26.88990788964548	14.683395674238009
7	66.14733209096286	18.69915806421484	10.016055933356757	5.1374539114655375
8	76.95951325170455	6.532229728828183	10.040517881684227	6.467739137783036
9	72.32197261151313	6.908498970374357	12.3145895507452	8.454938867367316
10	37.087427003322375	28.19884450651355	19.554436730282557	15.159291759881516
11	27.892847771071743	27.370696364954476	26.95084037911572	17.785615484858052
12	27.452087938480425	21.856528449245904	32.39962817838542	18.291755433888248
13	22.38446177042239	27.06292057872522	32.95380249867683	17.598815152175558
14	16.659476336400715	33.404791873296006	29.675011897402143	20.260719892901143
15	15.86424063440951	27.235043742411236	40.055328479489766	16.845387143689482
16	18.17122474303835	26.662634151548442	34.09550834152438	21.070632763888828
17	17.700221047060342	24.66476011723945	30.32658924830657	27.308429587393647
18	19.590462508728468	25.32478795938427	33.48218058255018	21.60256894933708
19	21.3059122305294	27.66735308376216	28.602244272568374	22.42449041314007
20	21.752898740876805	27.367583026076435	29.737278674962976	21.142239558083784
21	20.95543922540129	28.283349418917535	30.760232877748077	20.0009784779331
22	17.375544278350286	26.79606296060737	29.230693963235915	26.59769879780643
23	16.898758667313054	28.733004505446118	30.370620755296013	23.99761607194481
24	20.590733813973554	24.546453239873866	30.659716508257016	24.20309643789556
25	17.63484093062147	28.58045090042208	29.236031115598273	24.54867705335818
26	16.518486561495113	30.510276242111022	29.087480374846002	23.883756821547863
27	19.714551301153268	29.45974675212041	29.57805362948599	21.247648317240337
28	17.219877334448206	27.816348587211294	32.36360239993951	22.60017167840099
29	18.068929322759843	27.585071984842486	32.345811892064994	22.00018680033268
30	21.756012079754846	27.578845307086404	30.790031978437902	19.875110634720844
31	23.34870729722157	28.041398511824017	26.03596351166835	22.573930679286068
32	21.385079990571032	28.75168453871437	29.8791579752623	19.9840774954523
33	23.16590982881084	25.53071308803188	29.37123897544465	21.93213810771263
34	20.494665071451127	25.718402946108103	30.28211297862026	23.50481900382051
35	17.77938880710197	25.190024862234754	33.31539457122652	23.715191759436753
36	23.36471875430864	24.566912323929568	32.691837270224475	19.37653165153732
37	19.51841095183665	25.075720849140943	35.501403226308604	19.90446497271381
38	20.593402390154733	25.949234785780046	30.387966500473674	23.069396323591548
39	22.471635259007556	22.942194192288703	31.94419117679762	22.64197937190612
40	22.682897540017525	23.704072692015174	31.383790178750125	22.229239589217173
41	17.23188592726351	25.072607510262902	31.523890428262003	26.17161613421159
42	20.926529650105184	23.46301131031538	34.10351407006792	21.50694496951152
43	23.196598454894392	23.252193792002277	31.576372426491844	21.974835326611487
44	21.6083508643963	24.62606576261236	29.820894061973235	23.944689311018106
45	20.713043555610906	23.836167212983515	28.837078976512082	26.6137102548935
46	23.481246580886765	24.46506166634792	29.737278674962976	22.316413077802338
47	18.297092586250606	23.086297306072346	34.418850822143845	24.197759285533206
48	20.059687153919025	21.863644652395713	33.79573828383865	24.280929909846602
49	20.056573815040984	20.7468455205725	34.7773295558155	24.41925110857102
50	19.25066380832507	21.172483421470474	33.54533688550474	26.03151588469972
51	18.822357331245914	22.257259639119546	29.95565715912275	28.96472587051179
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	563.0
1	574.0
2	585.0
3	701.5
4	818.0
5	710.5
6	603.0
7	549.0
8	495.0
9	493.0
10	491.0
11	560.0
12	629.0
13	634.0
14	639.0
15	707.0
16	775.0
17	736.5
18	698.0
19	749.0
20	800.0
21	799.5
22	799.0
23	1064.0
24	1329.0
25	1514.5
26	2047.5
27	2395.0
28	2518.5
29	2642.0
30	2902.0
31	3162.0
32	3957.0
33	4752.0
34	5396.5
35	6041.0
36	6738.0
37	7435.0
38	8371.0
39	9307.0
40	11178.0
41	13049.0
42	16873.5
43	20698.0
44	25059.0
45	29420.0
46	28996.0
47	28572.0
48	26715.0
49	24858.0
50	20710.5
51	16563.0
52	14103.0
53	11643.0
54	10031.5
55	8420.0
56	7392.5
57	6365.0
58	5693.0
59	5021.0
60	4826.0
61	4631.0
62	4277.0
63	3923.0
64	2999.0
65	2075.0
66	1621.5
67	1168.0
68	920.0
69	672.0
70	554.0
71	436.0
72	370.0
73	304.0
74	235.0
75	120.0
76	74.0
77	63.0
78	52.0
79	42.0
80	32.0
81	22.0
82	12.0
83	9.0
84	6.0
85	7.5
86	9.0
87	6.5
88	4.0
89	5.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	224839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.697132207101845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05399994719622	33.546985583116445
2	10.549999992577613	9.431094889063301
3	4.189999997052151	5.618429514479891
4	2.2930000681557376	4.099621087890022
5	1.4449999995635816	3.2293678009877764
6	0.9739999993199072	2.6121004043591403
7	0.7369999994815396	2.305925048942226
8	0.5429999996179761	1.9416434217104748
9	0.40899999971225054	1.6453014353858775
>10	3.730999997375078	30.397625650019762
>50	0.04199999997045115	1.3342093954433154
>100	0.026999999981004313	2.1409926312138965
>500	0.003999999997185824	1.2394514752309258
>1k	9.99999999296456E-4	0.45725166215695534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG	1023	0.45499223889093976	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC	800	0.35581015749047096	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	781	0.3473596662500723	No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	635	0.2824243125080613	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	557	0.2477328221527404	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	415	0.1845765191981818	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	390	0.1734574517766046	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	285	0.12675736860598028	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	278	0.12364402972793866	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT	273	0.12142021624362322	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32779010758809635	0.0
2	0.0	0.0	0.0	1.579352336560828	0.0
3	0.0	0.0	0.0	2.1691076726012835	0.0
4	0.0	0.0	0.0	2.898518495456749	0.0
5	0.0	0.0	0.0	5.062289015695676	0.0
6	0.0	0.0	0.0	6.246247314745218	0.0
7	0.0	0.0	0.0	7.486245713599509	0.0
8	0.0	0.0	0.0	9.489456900270861	0.0
9	0.0	0.0	0.0	10.191737198617677	0.0
10	0.0	0.0	0.0	12.389754446515061	0.0
11	0.0	0.0	0.0	14.508603934370816	0.0
12	0.0	0.0	0.0	16.66347920067248	0.0
13	0.0	0.0	0.0	17.4982987826845	0.0
14	0.0	0.0	0.0	17.876791837714986	0.0
15	0.0	0.0	0.0	18.438082361156205	0.0
16	0.0	0.0	0.0	19.568669136582177	0.0
17	0.0	0.0	0.0	20.961221140460506	0.0
18	0.0	0.0	0.0	22.645982236177886	0.0
19	0.0	0.0	0.0	23.402523583542003	0.0
20	0.0	0.0	0.0	24.186195455414765	0.0
21	0.0	0.0	0.0	25.099293272074686	0.0
22	0.0	0.0	0.0	26.05019591796797	0.0
23	0.0	0.0	0.0	26.952619429903176	0.0
24	0.0	0.0	0.0	27.615760610926042	0.0
25	0.0	0.0	0.0	28.231312183384556	0.0
26	0.0	0.0	0.0	28.823291332909328	0.0
27	0.0	0.0	0.0	29.367236111172883	0.0
28	0.0	0.0	0.0	30.005025818474554	0.0
29	0.0	0.0	0.0	30.583217324396568	0.0
30	0.0	0.0	0.0	31.255698522053557	0.0
31	0.0	0.0	0.0	31.88503773811483	0.0
32	0.0	0.0	0.0	32.47212449797411	0.0
33	0.0	0.0	0.0	33.05698744434907	0.0
34	0.0	0.0	0.0	33.63829228914913	0.0
35	0.0	0.0	0.0	34.26985531869471	0.0
36	0.0	0.0	0.0	34.844043960344955	0.0
37	0.0	0.0	0.0	35.41022687345167	0.0
38	0.0	0.0	0.0	35.960843092168176	0.0
39	0.0	0.0	0.0	36.52346790369998	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCGG	20	7.021168E-4	45.000004	2
CGGGTTC	20	7.021168E-4	45.000004	6
GTCATAA	20	7.021168E-4	45.000004	18
CGGGTAT	30	2.1576889E-6	45.000004	6
GGAGCGC	20	7.021168E-4	45.000004	8
ACTGCGG	20	7.021168E-4	45.000004	2
CATATTA	30	2.1576889E-6	45.000004	28
GGTCGTT	20	7.021168E-4	45.000004	8
CCTAGTC	20	7.021168E-4	45.000004	27
GACGTTG	20	7.021168E-4	45.000004	1
CCGGAGA	20	7.021168E-4	45.000004	16
ATTCGCA	20	7.021168E-4	45.000004	38
TACGGGC	20	7.021168E-4	45.000004	4
TGCGGAG	20	7.021168E-4	45.000004	1
CGTTATT	30	2.1576889E-6	45.000004	1
ATACTGG	20	7.021168E-4	45.000004	2
TACTGAC	20	7.021168E-4	45.000004	20
GCGGGTC	30	2.1576889E-6	45.000004	5
ATGACGG	20	7.021168E-4	45.000004	2
CAAGCCC	30	2.1576889E-6	45.000004	10
>>END_MODULE