##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936125.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320247
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.795470371307147	31.0	31.0	34.0	30.0	34.0
2	31.922872033149414	33.0	31.0	34.0	30.0	34.0
3	31.87613310975591	33.0	31.0	34.0	30.0	34.0
4	35.61344212436026	37.0	35.0	37.0	33.0	37.0
5	31.211283790324345	37.0	35.0	37.0	0.0	37.0
6	33.38662657261426	37.0	35.0	37.0	17.0	37.0
7	29.458411788400827	35.0	32.0	37.0	0.0	37.0
8	32.507111698157985	35.0	33.0	37.0	17.0	37.0
9	36.39102942416322	39.0	35.0	39.0	32.0	39.0
10	36.73355878431336	38.0	35.0	39.0	33.0	39.0
11	36.83979865541286	39.0	37.0	39.0	33.0	39.0
12	36.83434349111779	39.0	35.0	39.0	33.0	39.0
13	36.57405377724069	39.0	35.0	39.0	32.0	39.0
14	37.792747473044244	40.0	37.0	41.0	33.0	41.0
15	37.864648224651596	40.0	36.0	41.0	33.0	41.0
16	37.957489063129394	40.0	36.0	41.0	33.0	41.0
17	37.919340384140995	40.0	36.0	41.0	33.0	41.0
18	37.83247930503642	40.0	36.0	41.0	33.0	41.0
19	37.73652836716659	39.0	36.0	41.0	33.0	41.0
20	37.71970385358801	39.0	36.0	41.0	33.0	41.0
21	37.660115473369	39.0	36.0	41.0	33.0	41.0
22	37.658051441543556	39.0	36.0	41.0	33.0	41.0
23	37.63369211889573	39.0	36.0	41.0	33.0	41.0
24	37.55502471529788	39.0	36.0	41.0	33.0	41.0
25	37.44879733455739	39.0	35.0	41.0	33.0	41.0
26	37.29073184135995	39.0	35.0	41.0	32.0	41.0
27	37.27231168441859	39.0	35.0	41.0	32.0	41.0
28	37.06694520167246	39.0	35.0	41.0	32.0	41.0
29	37.078295815417476	39.0	35.0	41.0	31.0	41.0
30	36.97162502693234	39.0	35.0	41.0	31.0	41.0
31	36.657714201850446	39.0	35.0	40.0	30.0	41.0
32	36.589994597919734	39.0	35.0	40.0	30.0	41.0
33	36.47830580770468	39.0	35.0	40.0	30.0	41.0
34	36.24435201578781	39.0	35.0	40.0	30.0	41.0
35	36.216857613029944	39.0	35.0	40.0	30.0	41.0
36	35.789912161550305	38.0	35.0	40.0	27.0	41.0
37	35.679085206106535	38.0	35.0	40.0	27.0	41.0
38	35.427685505250636	38.0	34.0	40.0	26.0	41.0
39	35.37566940517788	38.0	34.0	40.0	25.0	41.0
40	35.30914887571156	38.0	34.0	40.0	25.0	41.0
41	35.00348793275191	38.0	34.0	40.0	24.0	41.0
42	35.0102483395629	38.0	34.0	40.0	24.0	41.0
43	34.71001133500079	38.0	34.0	40.0	23.0	41.0
44	34.44102520866706	38.0	33.0	40.0	22.0	41.0
45	34.282747379366555	38.0	33.0	40.0	22.0	41.0
46	33.962628845859605	38.0	33.0	40.0	20.0	41.0
47	33.75264717546144	37.0	33.0	40.0	19.0	41.0
48	33.61187146171549	37.0	32.0	40.0	18.0	41.0
49	33.51083382514122	37.0	32.0	40.0	18.0	41.0
50	33.31828869591284	37.0	32.0	40.0	17.0	41.0
51	31.043572617385955	35.0	28.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	9.0
11	10.0
12	3.0
13	3.0
14	6.0
15	15.0
16	31.0
17	69.0
18	123.0
19	258.0
20	475.0
21	755.0
22	1345.0
23	1840.0
24	2745.0
25	3638.0
26	4435.0
27	5292.0
28	6114.0
29	7080.0
30	8451.0
31	10239.0
32	12736.0
33	17067.0
34	24211.0
35	28887.0
36	31170.0
37	46601.0
38	55914.0
39	50702.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.15608264870553	19.741324665024184	24.35089165550341	10.751701030766876
2	30.008087507455183	28.13016203118218	25.263936898706312	16.597813562656324
3	30.93268633273692	27.0975216005146	26.784013589510597	15.185778477237882
4	26.555440019734768	25.887205813013082	29.354841731538468	18.202512435713682
5	23.250803286213454	38.33478533756757	21.423776022882336	16.990635353336643
6	25.046916911009315	36.495892233182516	24.021146177794016	14.436044678014156
7	68.60111101743341	19.48808263621517	8.397892876436002	3.512913469915409
8	82.19780357036912	6.928089880623394	7.039566334735377	3.8345402142721086
9	77.06957442224282	6.785075270025949	8.36822827380116	7.77712203393006
10	40.201469490736834	32.5483142699229	15.131133156594753	12.119083082745506
11	29.60683472444707	25.076269254669054	27.59526240682973	17.721633614054152
12	28.163261482543163	22.8548589057821	30.061483792197897	18.92039581947684
13	23.353536489022535	26.52952252480117	32.22450171274048	17.892439273435816
14	20.090117940214895	30.626984796110502	28.073330897713326	21.209566365961273
15	18.061371378966857	26.836473097328	37.35023278906594	17.7519227346392
16	21.659843808060653	26.425540286091675	31.25618663094424	20.658429274903433
17	21.047816216857612	24.66939581010907	30.36780984677452	23.91497812625879
18	22.319647022454543	25.77947646660234	31.080072568985813	20.8208039419573
19	22.121674832238867	28.438673898584533	28.283481187958042	21.15617008121856
20	24.112325798524264	27.756388037983182	30.523314816376107	17.607971347116443
21	22.7680509107033	28.696599812020096	29.655547124563224	18.879802152713374
22	21.640171492629126	25.461596829946885	29.0444563102855	23.853775367138493
23	20.312446330488655	28.92798371257186	28.736881219808456	22.022688737131027
24	21.448444481915523	25.901569725867844	29.96811835864192	22.68186743357471
25	19.1864404662651	29.625882521928386	28.832432466190163	22.35524454561635
26	19.983013111754364	29.330485531480388	27.96903640002873	22.717464956736517
27	21.067800791264244	30.357193041621	27.965289292327483	20.609716874787274
28	18.227180894746866	27.46910978088788	33.28961707681883	21.014092247546426
29	22.02830939868289	25.05347434948649	30.902709471126972	22.015506780703646
30	22.333074158383997	27.035382064468987	30.78842268623906	19.843121090907957
31	24.9760341236608	26.180729249610458	27.581835270900275	21.26140135582847
32	24.307799916939114	27.838824407410527	28.50487280130649	19.348502874343865
33	22.836123367275885	25.632714748303652	28.24819592377134	23.282965960649125
34	24.525444422586315	25.658007725287046	29.162490202874658	20.654057649251982
35	22.81832460569498	24.47798105837057	29.836969589098416	22.866724746836038
36	25.695478802299476	25.283296955162733	28.612289888742126	20.40893435379566
37	23.039403960068324	25.58587590203811	31.89069686835474	19.484023269538824
38	19.356309348721457	26.525463158124822	31.283353161778255	22.834874331375467
39	22.828629151873397	24.858000231071642	31.330816525994	20.982554091060962
40	23.04783495239612	23.812244923449715	31.7392512654295	21.40066885872467
41	21.3510196816832	24.430517694154823	29.390751513675383	24.827711110486593
42	22.093883783454647	24.038632680399814	31.029174356043924	22.83830918010161
43	22.347750330213866	24.632236992071746	28.780285217347863	24.23972746036653
44	21.988652508844737	24.59882528173566	30.059610238347272	23.35291197107233
45	21.282947225110618	25.18712119083083	28.810262078957805	24.71966950510075
46	23.528401515080546	26.2978263652743	29.099101630928626	21.07467048871652
47	20.526968246384822	24.25346685527109	33.01639047360319	22.203174424740904
48	21.332908661127192	24.104519324146676	31.367663085056225	23.19490892966991
49	21.140244873488275	21.823155252039832	33.28212286141635	23.754477013055546
50	20.78770449059632	24.340899368300093	31.318638425964956	23.55275771513863
51	20.59628973885782	23.359781668524608	29.254294341555116	26.78963425106246
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	376.0
1	505.0
2	634.0
3	835.5
4	1037.0
5	869.0
6	701.0
7	644.0
8	587.0
9	619.5
10	652.0
11	611.5
12	571.0
13	609.5
14	648.0
15	707.0
16	766.0
17	836.0
18	906.0
19	968.0
20	1030.0
21	1210.5
22	1391.0
23	1686.5
24	1982.0
25	2347.0
26	2983.5
27	3255.0
28	3884.0
29	4513.0
30	5245.5
31	5978.0
32	6932.0
33	7886.0
34	8849.0
35	9812.0
36	11111.5
37	12411.0
38	13461.5
39	14512.0
40	15936.5
41	17361.0
42	19492.0
43	21623.0
44	24363.0
45	27103.0
46	30794.0
47	34485.0
48	32581.5
49	30678.0
50	29363.5
51	28049.0
52	24993.5
53	21938.0
54	18953.0
55	15968.0
56	14161.0
57	12354.0
58	11421.0
59	10488.0
60	9738.0
61	8988.0
62	8213.0
63	7438.0
64	5948.5
65	4459.0
66	3607.0
67	2755.0
68	2169.0
69	1583.0
70	1268.5
71	954.0
72	786.0
73	618.0
74	520.5
75	312.5
76	202.0
77	189.0
78	176.0
79	149.0
80	122.0
81	89.0
82	56.0
83	43.0
84	30.0
85	25.0
86	20.0
87	14.0
88	8.0
89	6.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	320247.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.625140367908415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.20923326488355	31.72220031981453
2	8.47686128655429	7.057010818642221
3	3.6574533999989534	4.56726033492121
4	2.2973377525780383	3.825080256942244
5	1.5541725776469208	3.2346325850253557
6	1.1666171169632642	2.9136360749520303
7	0.8283325391145825	2.413562074836534
8	0.6082067598285137	2.025335340045811
9	0.49894873865500333	1.8691930154614826
>10	4.643742623013374	36.57715700936047
>50	0.03515715463147029	0.9381465891208007
>100	0.02094468786555677	1.551849518811175
>500	0.001496049133254055	0.4178535421839079
>1k	0.001496049133254055	0.8870825198822306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	1540	0.4808788216595315	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	1309	0.4087469984106018	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	809	0.25261751085880585	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	533	0.16643403373021448	RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	356	0.11116419513687871	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	325	0.10148416690866736	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1548804516513816	0.0
2	0.0	0.0	0.0	0.8777599790161968	0.0
3	0.0	0.0	0.0	1.085099938484982	0.0
4	0.0	0.0	0.0	1.421715113646654	0.0
5	0.0	0.0	0.0	2.7007278756709665	0.0
6	0.0	0.0	0.0	3.1375781818408917	0.0
7	0.0	0.0	0.0	3.759285801272143	0.0
8	0.0	0.0	0.0	4.6763904111513925	0.0
9	0.0	0.0	0.0	5.014254622213479	0.0
10	0.0	0.0	0.0	6.9708693602125855	0.0
11	0.0	0.0	0.0	8.266119588942285	0.0
12	0.0	0.0	0.0	10.465359550596883	0.0
13	0.0	0.0	0.0	10.950609997907865	0.0
14	0.0	0.0	0.0	11.15888673430196	0.0
15	0.0	0.0	0.0	11.615097096928308	0.0
16	0.0	0.0	0.0	12.32017786271222	0.0
17	0.0	0.0	0.0	13.273504513703486	0.0
18	0.0	0.0	0.0	14.266488054532907	0.0
19	0.0	0.0	0.0	14.814190296864608	0.0
20	0.0	0.0	0.0	15.355335100719133	0.0
21	0.0	0.0	0.0	15.997651812507222	0.0
22	0.0	0.0	0.0	16.6477749986729	0.0
23	0.0	0.0	0.0	17.34598606700453	0.0
24	0.0	0.0	0.0	17.872142440054084	0.0
25	0.0	0.0	0.0	18.325542471904498	0.0
26	0.0	0.0	0.0	18.77332184220305	0.0
27	0.0	0.0	0.0	19.201428897070073	0.0
28	0.0	0.0	0.0	19.634219836563652	0.0
29	0.0	0.0	0.0	20.09729989664228	0.0
30	0.0	0.0	0.0	20.623456269691832	0.0
31	3.1225897510359194E-4	0.0	0.0	21.11963578113144	0.0
32	3.1225897510359194E-4	0.0	0.0	21.59957782586566	0.0
33	3.1225897510359194E-4	0.0	0.0	22.06640499364553	0.0
34	3.1225897510359194E-4	0.0	0.0	22.553528994807134	0.0
35	3.1225897510359194E-4	0.0	0.0	23.039716219043427	0.0
36	3.1225897510359194E-4	0.0	0.0	23.510915012474747	0.0
37	3.1225897510359194E-4	0.0	0.0	24.009592595715183	0.0
38	3.1225897510359194E-4	0.0	0.0	24.484850755822848	0.0
39	3.1225897510359194E-4	0.0	0.0	24.983840598038388	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	35	1.2082637E-7	45.000004	4
TTAGCGG	25	3.8844813E-5	45.000004	2
CGGTTGA	20	7.0256554E-4	45.000004	33
GGCGTAT	20	7.0256554E-4	45.000004	9
ATTCGCG	20	7.0256554E-4	45.000004	1
CACGACC	35	1.2082637E-7	45.000004	27
CTAACGG	25	3.8844813E-5	45.000004	2
TGACCGG	30	2.1605792E-6	45.000004	2
TGTTCGA	30	2.1605792E-6	45.000004	34
CTTTGCG	20	7.0256554E-4	45.000004	1
GCCTATC	20	7.0256554E-4	45.000004	3
GGTGCGT	20	7.0256554E-4	45.000004	41
GCGGCCA	25	3.8844813E-5	45.000004	22
TACTACG	20	7.0256554E-4	45.000004	35
AAGTGCG	35	1.2082637E-7	45.000004	1
GCGTATT	20	7.0256554E-4	45.000004	10
TATGGGA	270	0.0	42.5	4
GTTGATG	65	0.0	41.538464	1
CGCATGG	60	3.6379788E-12	41.250004	2
AACGGGA	115	0.0	41.086956	4
>>END_MODULE