FastQCFastQC Report
Sat 14 Jan 2017
SRR2936124.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936124.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325895
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.11384034735114071No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC302.1606975E-645.00000410
ACGAACG302.1606975E-645.0000042
GCGACGG207.0258393E-445.02
CCGATCA253.8846334E-545.011
TAGACGG351.2083547E-745.02
GTGATCA207.0258393E-445.035
CTACGAA253.8846334E-545.011
TGCGACG207.0258393E-445.01
AACGAAC207.0258393E-445.01
AGTACGG406.7884685E-945.02
ATGGGAC1200.041.2500045
GGACCGA1300.039.807698
CCCTAGC1250.039.621
CGTATGG403.448058E-739.3752
CGATGCA403.448058E-739.37510
AACACGG403.448058E-739.3752
ACGGGAC750.039.05
GAGTACC356.2345207E-638.57142629
GTATCGG356.2345207E-638.5714262
ATCGCGG356.2345207E-638.5714262
Produced by FastQC (version 0.11.5)