##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936124.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	325895
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.901753632304885	31.0	31.0	34.0	30.0	34.0
2	32.04747848233327	33.0	31.0	34.0	30.0	34.0
3	31.996980622593167	33.0	31.0	34.0	30.0	34.0
4	35.72131514751684	37.0	35.0	37.0	35.0	37.0
5	31.187477561791376	37.0	35.0	37.0	0.0	37.0
6	33.405925221313616	37.0	35.0	37.0	17.0	37.0
7	29.488169502447107	35.0	32.0	37.0	0.0	37.0
8	32.57005784071557	35.0	33.0	37.0	17.0	37.0
9	36.46799122416729	39.0	35.0	39.0	32.0	39.0
10	36.800239340892006	38.0	35.0	39.0	33.0	39.0
11	36.909384924592274	39.0	37.0	39.0	33.0	39.0
12	36.8633118028813	39.0	35.0	39.0	33.0	39.0
13	36.61872995903589	39.0	35.0	39.0	33.0	39.0
14	37.83826385799107	40.0	37.0	41.0	33.0	41.0
15	37.97536016201537	40.0	37.0	41.0	33.0	41.0
16	38.03502355053008	40.0	37.0	41.0	33.0	41.0
17	38.0030715414474	40.0	36.0	41.0	33.0	41.0
18	37.91409503060802	40.0	36.0	41.0	33.0	41.0
19	37.82332960002455	40.0	36.0	41.0	33.0	41.0
20	37.752625845747865	40.0	36.0	41.0	33.0	41.0
21	37.63779438162599	39.0	36.0	41.0	33.0	41.0
22	37.62289080838921	39.0	36.0	41.0	33.0	41.0
23	37.57399162306878	39.0	36.0	41.0	33.0	41.0
24	37.51504318875711	39.0	35.0	41.0	33.0	41.0
25	37.38829991254852	39.0	35.0	41.0	32.0	41.0
26	37.22111109406404	39.0	35.0	41.0	32.0	41.0
27	37.16871384955277	39.0	35.0	41.0	32.0	41.0
28	37.08352997130978	39.0	35.0	41.0	32.0	41.0
29	37.044146120683045	39.0	35.0	41.0	32.0	41.0
30	36.87395019868362	39.0	35.0	41.0	31.0	41.0
31	36.69653109130241	39.0	35.0	41.0	31.0	41.0
32	36.588235474616056	39.0	35.0	40.0	30.0	41.0
33	36.43004035041962	39.0	35.0	40.0	30.0	41.0
34	36.28099234415993	39.0	35.0	40.0	30.0	41.0
35	36.178640359625035	39.0	35.0	40.0	30.0	41.0
36	35.99948142806732	38.0	35.0	40.0	29.0	41.0
37	35.88151398456558	38.0	35.0	40.0	29.0	41.0
38	35.795093511713894	38.0	35.0	40.0	28.0	41.0
39	35.7557648936007	38.0	35.0	40.0	28.0	41.0
40	35.61161110173522	38.0	35.0	40.0	27.0	41.0
41	35.50630417772595	38.0	34.0	40.0	27.0	41.0
42	35.45444698445819	38.0	34.0	40.0	27.0	41.0
43	35.27293760260206	38.0	34.0	40.0	26.0	41.0
44	35.09828625784378	38.0	34.0	40.0	26.0	41.0
45	34.936362938983414	38.0	34.0	40.0	25.0	41.0
46	34.80359318185305	38.0	34.0	40.0	24.0	41.0
47	34.61936513294159	37.0	33.0	40.0	24.0	41.0
48	34.535988585280535	37.0	33.0	40.0	23.0	41.0
49	34.39759738566102	37.0	33.0	40.0	24.0	41.0
50	34.21494653185842	37.0	33.0	40.0	23.0	41.0
51	32.2579603860139	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	22.0
10	17.0
11	21.0
12	16.0
13	9.0
14	14.0
15	26.0
16	47.0
17	118.0
18	192.0
19	308.0
20	512.0
21	809.0
22	1237.0
23	1671.0
24	2256.0
25	3049.0
26	3617.0
27	4401.0
28	5234.0
29	6137.0
30	7317.0
31	9318.0
32	12158.0
33	17529.0
34	25101.0
35	29844.0
36	33551.0
37	50059.0
38	59135.0
39	52151.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06112398165053	20.76098129765722	24.01816536000859	11.159729360683656
2	31.782629374491783	24.817195722548675	25.88103530278157	17.519139600177972
3	33.411374829316195	23.818714616671013	26.252934227281795	16.516976326731005
4	28.927722119087434	26.221022108347782	25.706132343239386	19.1451234293254
5	22.36211049571181	39.2927169793952	21.13380076404977	17.211371760843218
6	26.153515702910447	35.768575768268924	24.013562650546955	14.064345878273677
7	70.43710397520674	19.612145016032773	6.14001442182298	3.8107365869375105
8	84.77086178063486	5.148590803786496	6.601205909878949	3.4793415056996886
9	79.60478068089415	6.486751867932923	8.534650731063687	5.373816720109238
10	44.2872704398656	24.776998726583717	15.593059114131853	15.342671719418831
11	31.65191242578131	25.671765445925836	23.544699980054926	19.131622148237927
12	28.19497077279492	23.727887816628055	28.97466975559613	19.1024716549809
13	24.464935025084767	26.271958759723223	29.884471992512925	19.378634222679082
14	20.692247503030117	28.54140137160742	29.88846100737968	20.877890117982787
15	20.685190015188944	25.123429325396216	34.36781785544424	19.823562803970603
16	24.026143389742096	24.344650884487333	30.916706301109254	20.712499424661317
17	24.106844228969454	24.102855214102703	28.06762914435631	23.722671412571533
18	25.855566977093847	24.818729959035885	29.69085134782675	19.63485171604351
19	26.501173690912715	26.512220193620646	26.00223998527133	20.98436613019531
20	26.36339925436107	26.935669464091195	27.020666165482748	19.68026511606499
21	26.417404378710934	26.36401294895595	27.49720001841084	19.721382653922277
22	25.31889105386704	24.037189892450023	27.685297411743047	22.95862164193989
23	23.48701268813575	26.339158317863117	27.79913775909419	22.374691234906948
24	23.701498949048005	24.85708587121619	29.379708188220132	22.061706991515674
25	23.08258795010663	26.68589576397306	28.136976633578296	22.094539652342014
26	22.640114147194648	27.80466101044815	26.40574418140812	23.149480660949077
27	22.2774206416177	26.81354423970911	29.151413798922967	21.757621319750225
28	20.926065143681246	26.734991331563844	29.9421592844321	22.396784240322802
29	22.324981972721275	26.17039230426978	28.762638273063413	22.741987449945533
30	23.38329830160021	26.283005262431153	28.69758664600561	21.636109789963026
31	26.493502508476656	26.046732843400484	26.348056889488948	21.111707758633916
32	25.87735313521226	26.712284631553107	25.996716733917363	21.413645499317266
33	23.98533269918225	26.43673575844981	26.28730112459535	23.290630417772597
34	23.311802881296124	26.397152457079738	27.722119087436138	22.568925574188007
35	23.528130225993035	26.25907117323064	27.588333665751243	22.624464935025085
36	25.0856871078108	26.76322128292855	26.586170392304272	21.56492121695638
37	23.485478451648536	27.973120176744043	27.189432179076086	21.351969192531335
38	23.219748692063394	28.185151659276762	25.91202687982326	22.68307276883659
39	23.30996179751147	26.80464566808328	26.241274029978985	23.644118504426274
40	24.468617192654076	26.151060924530906	27.457309869743323	21.923012013071695
41	21.25868761410884	26.287914819190227	27.617177311710826	24.836220254990103
42	23.615274858466684	26.116694027217356	26.56499792878074	23.70303318553522
43	23.662222494975378	24.44621733994078	27.754338053667592	24.137222111416254
44	23.16083401095445	25.305389772779574	27.9301615551021	23.60361466116387
45	23.656085549026525	26.361865017873853	26.169778609674893	23.812270823424722
46	23.836511759922672	26.55057610580095	26.91234906948557	22.700563064790806
47	22.831586860798726	26.143389742094847	27.953481949707726	23.0715414473987
48	23.05865386090612	25.51864864450206	28.683471670323264	22.73922582426855
49	23.218521302873622	25.149204498381376	28.629773393270842	23.002500805474156
50	21.135641847834425	26.05992727719051	28.828917289310972	23.975513585664093
51	21.89478206170699	25.885331164945764	27.4318415440556	24.788045229291644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	346.0
1	563.0
2	780.0
3	957.5
4	1135.0
5	902.5
6	670.0
7	602.0
8	534.0
9	590.0
10	646.0
11	630.0
12	614.0
13	631.5
14	649.0
15	656.5
16	664.0
17	688.5
18	713.0
19	788.0
20	863.0
21	993.5
22	1124.0
23	1449.0
24	1774.0
25	2080.5
26	2848.5
27	3310.0
28	3822.0
29	4334.0
30	5470.5
31	6607.0
32	6860.0
33	7113.0
34	8265.0
35	9417.0
36	10414.5
37	11412.0
38	12314.5
39	13217.0
40	14281.0
41	15345.0
42	16911.5
43	18478.0
44	19376.0
45	20274.0
46	21242.0
47	22210.0
48	23764.0
49	25318.0
50	25649.5
51	25981.0
52	24985.5
53	23990.0
54	22163.0
55	20336.0
56	18930.0
57	17524.0
58	16748.0
59	15972.0
60	14611.0
61	13250.0
62	12205.5
63	11161.0
64	9897.5
65	8634.0
66	7432.0
67	6230.0
68	5310.5
69	4391.0
70	3818.5
71	3246.0
72	2786.0
73	2326.0
74	1807.0
75	1014.5
76	741.0
77	546.0
78	351.0
79	269.0
80	187.0
81	157.0
82	127.0
83	120.0
84	113.0
85	79.5
86	46.0
87	38.5
88	31.0
89	22.0
90	13.0
91	14.0
92	15.0
93	10.0
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	325895.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.31184537744083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.180035245349	27.653503496580406
2	8.802930351677533	6.745130130037011
3	4.3371727269344795	4.984952726687015
4	2.8400822200390587	4.352351634934212
5	1.899308827459915	3.6383013060826355
6	1.3968553790956943	3.210966437913645
7	1.0495560660269934	2.814730080160688
8	0.8288733301217597	2.5404533488887466
9	0.6753947457845884	2.3288057162311597
>10	5.945830815419708	40.17154222089008
>50	0.03516823367302381	0.9091617350419893
>100	0.008792058418255953	0.6501011665524228
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	371	0.11384034735114071	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07302965679129782	0.0
2	0.0	0.0	0.0	0.3267923717761856	0.0
3	0.0	0.0	0.0	0.441860108317096	0.0
4	0.0	0.0	0.0	0.5931358259562128	0.0
5	0.0	0.0	0.0	1.1212200248546311	0.0
6	0.0	0.0	0.0	1.4461713128461622	0.0
7	0.0	0.0	0.0	1.7852375765200448	0.0
8	0.0	0.0	0.0	2.369167983552985	0.0
9	0.0	0.0	0.0	2.5864158701422237	0.0
10	0.0	0.0	0.0	3.2492060326178676	0.0
11	0.0	0.0	0.0	4.005584620813452	0.0
12	0.0	0.0	0.0	4.675432271130272	0.0
13	0.0	0.0	0.0	4.920910109084215	0.0
14	0.0	0.0	0.0	5.024317648322312	0.0
15	0.0	0.0	0.0	5.233894352475491	0.0
16	0.0	0.0	0.0	5.672072293223278	0.0
17	0.0	0.0	0.0	6.176836097516071	0.0
18	0.0	0.0	0.0	6.728854385614999	0.0
19	0.0	0.0	0.0	7.045213949278142	0.0
20	0.0	0.0	0.0	7.364948833213152	0.0
21	0.0	0.0	0.0	7.769987265837156	0.0
22	0.0	0.0	0.0	8.179321560625354	0.0
23	0.0	0.0	0.0	8.629773393270838	0.0
24	0.0	0.0	0.0	8.938768621795363	0.0
25	0.0	0.0	0.0	9.265867840868992	0.0
26	0.0	0.0	0.0	9.530370211264364	0.0
27	0.0	0.0	0.0	9.814510808696053	0.0
28	0.0	0.0	0.0	10.105708893968917	0.0
29	0.0	0.0	0.0	10.426364319796253	0.0
30	0.0	0.0	0.0	10.777397628070391	0.0
31	0.0	0.0	0.0	11.096825664707959	0.0
32	0.0	0.0	0.0	11.396922321606652	0.0
33	0.0	0.0	0.0	11.732613265008668	0.0
34	0.0	0.0	0.0	12.068304208410684	0.0
35	0.0	0.0	0.0	12.429770324797865	0.0
36	0.0	0.0	0.0	12.749198361435433	0.0
37	0.0	0.0	0.0	13.058500437257399	0.0
38	0.0	0.0	0.0	13.385906503628469	0.0
39	0.0	0.0	0.0	13.745224688933552	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGATC	30	2.1606975E-6	45.000004	10
ACGAACG	30	2.1606975E-6	45.000004	2
GCGACGG	20	7.0258393E-4	45.0	2
CCGATCA	25	3.8846334E-5	45.0	11
TAGACGG	35	1.2083547E-7	45.0	2
GTGATCA	20	7.0258393E-4	45.0	35
CTACGAA	25	3.8846334E-5	45.0	11
TGCGACG	20	7.0258393E-4	45.0	1
AACGAAC	20	7.0258393E-4	45.0	1
AGTACGG	40	6.7884685E-9	45.0	2
ATGGGAC	120	0.0	41.250004	5
GGACCGA	130	0.0	39.80769	8
CCCTAGC	125	0.0	39.6	21
CGTATGG	40	3.448058E-7	39.375	2
CGATGCA	40	3.448058E-7	39.375	10
AACACGG	40	3.448058E-7	39.375	2
ACGGGAC	75	0.0	39.0	5
GAGTACC	35	6.2345207E-6	38.571426	29
GTATCGG	35	6.2345207E-6	38.571426	2
ATCGCGG	35	6.2345207E-6	38.571426	2
>>END_MODULE