##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936123.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	884995
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8936129582653	31.0	31.0	34.0	30.0	34.0
2	32.0168419030616	33.0	31.0	34.0	30.0	34.0
3	31.765098107898915	33.0	31.0	34.0	30.0	34.0
4	35.60504296634444	37.0	35.0	37.0	33.0	37.0
5	31.073530358928583	37.0	35.0	37.0	0.0	37.0
6	33.34935790597687	37.0	35.0	37.0	17.0	37.0
7	29.590080169944464	35.0	32.0	37.0	0.0	37.0
8	32.61810744693473	35.0	33.0	37.0	17.0	37.0
9	36.497251396900545	39.0	35.0	39.0	32.0	39.0
10	36.86498115808564	38.0	37.0	39.0	33.0	39.0
11	36.9516618737959	39.0	37.0	39.0	33.0	39.0
12	36.935104718105755	39.0	37.0	39.0	33.0	39.0
13	36.74951835886078	39.0	35.0	39.0	33.0	39.0
14	37.92237018288239	40.0	37.0	41.0	33.0	41.0
15	38.05666359696947	40.0	37.0	41.0	33.0	41.0
16	38.124225560596386	40.0	37.0	41.0	33.0	41.0
17	38.088377900440115	40.0	37.0	41.0	33.0	41.0
18	37.98167899253668	40.0	37.0	41.0	33.0	41.0
19	37.84901383623636	40.0	37.0	41.0	33.0	41.0
20	37.70771134300194	40.0	36.0	41.0	33.0	41.0
21	37.6090633280414	39.0	36.0	41.0	33.0	41.0
22	37.59636156136475	39.0	35.0	41.0	33.0	41.0
23	37.52207300606218	39.0	35.0	41.0	33.0	41.0
24	37.45185679015135	39.0	35.0	41.0	33.0	41.0
25	37.297124842513234	39.0	35.0	41.0	32.0	41.0
26	37.11435431838598	39.0	35.0	41.0	32.0	41.0
27	37.079269374403246	39.0	35.0	41.0	32.0	41.0
28	36.92007864451212	39.0	35.0	41.0	31.0	41.0
29	36.85096638964062	39.0	35.0	41.0	31.0	41.0
30	36.69250899722597	39.0	35.0	41.0	31.0	41.0
31	36.53742111537353	39.0	35.0	41.0	30.0	41.0
32	36.39283950756784	39.0	35.0	40.0	30.0	41.0
33	36.16819304063865	39.0	35.0	40.0	30.0	41.0
34	35.9094593754767	39.0	35.0	40.0	28.0	41.0
35	35.72644930197346	38.0	35.0	40.0	27.0	41.0
36	35.53057135916022	38.0	35.0	40.0	26.0	41.0
37	35.38709258244397	38.0	34.0	40.0	25.0	41.0
38	35.2007062186792	38.0	34.0	40.0	25.0	41.0
39	35.1189735535229	38.0	34.0	40.0	24.0	41.0
40	34.977512867304334	38.0	34.0	40.0	24.0	41.0
41	34.843099678529256	38.0	34.0	40.0	23.0	41.0
42	34.78885869411692	38.0	34.0	40.0	23.0	41.0
43	34.65399465533704	38.0	34.0	40.0	23.0	41.0
44	34.49224458895248	38.0	33.0	40.0	23.0	41.0
45	34.388683551884476	38.0	33.0	40.0	23.0	41.0
46	34.31506957666428	37.0	33.0	40.0	23.0	41.0
47	34.163712789337794	37.0	33.0	40.0	22.0	41.0
48	34.04712681992554	37.0	33.0	40.0	22.0	41.0
49	33.91369555760202	37.0	33.0	40.0	21.0	41.0
50	33.72412838490613	37.0	33.0	40.0	20.0	41.0
51	31.823584314035674	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	22.0
9	47.0
10	32.0
11	47.0
12	26.0
13	40.0
14	47.0
15	98.0
16	180.0
17	321.0
18	626.0
19	1032.0
20	1714.0
21	2658.0
22	3989.0
23	5978.0
24	8428.0
25	11452.0
26	14050.0
27	15309.0
28	16242.0
29	17674.0
30	21007.0
31	25567.0
32	32397.0
33	45830.0
34	64398.0
35	75025.0
36	87023.0
37	131527.0
38	156065.0
39	146134.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.29854405957096	19.94497144051661	23.6601336730716	14.096350826840828
2	34.0964638218295	23.53120639099656	25.48714964491325	16.88518014226069
3	32.57713320414239	23.127136311504586	28.068407166142183	16.227323318210836
4	27.575862010519835	25.272798151401986	28.90750795202233	18.243831886055855
5	21.923965672122442	38.60360792998831	23.24736297945186	16.22506341843739
6	25.368391911818712	34.38539200786445	26.22060011638484	14.025615963932001
7	68.39586664331438	19.09807400041808	8.846490658139311	3.6595686981282376
8	83.06747495748563	4.608952592952503	9.147848292928208	3.1757241566336534
9	77.50314973530924	6.531675320199549	10.94085277317951	5.024322171311702
10	43.79482369956892	24.32544816637382	17.978971632608094	13.90075650144916
11	31.078367674393643	25.628054395787547	26.005344662964198	17.288233266854615
12	28.02117526087718	22.829959491296563	30.461302041254466	18.687563206571788
13	24.724094486409527	22.72193628212589	32.56470375538845	19.989265476076138
14	21.403397759309374	24.52691823117645	32.991146842637534	21.07853716687665
15	20.931417691625377	24.40601359329714	34.35386640602489	20.30870230905259
16	24.925112571257465	23.34261775490257	31.33407533375895	20.398194340081016
17	24.52759620110848	24.054034203583072	30.20435143701377	21.214018158294678
18	25.322515946417777	23.66465347261849	31.14311380290284	19.86971677806089
19	24.886920265086243	26.024779801015825	28.207729987175068	20.88056994672286
20	25.481047915524947	26.435403589850793	29.027282640014917	19.056265854609347
21	25.41257295238956	25.563873242221707	30.0222035152741	19.001350290114633
22	25.114492172272158	23.29741975943367	30.15598958186205	21.43209848643213
23	23.5367431454415	24.763303747478798	30.58921236843146	21.11074073864824
24	23.539116040203616	24.643755049463557	30.837349363555727	20.9797795467771
25	23.846575404380815	26.13833976463144	28.39891750800852	21.616167322979226
26	22.24803529963446	25.336075345058447	29.314515901219778	23.101373454087312
27	22.347357894677373	25.365341047124563	30.37418290498816	21.913118153209904
28	21.349386154724037	25.852123458324623	29.994406748060726	22.804083638890617
29	22.05684777880101	25.360482262611654	29.988644003638438	22.594025954948897
30	23.047474844490647	25.21618766207719	30.078475019632876	21.657862473799288
31	24.024655506528287	25.825569635986646	28.438352759055135	21.711422098429935
32	25.031553850586725	25.63178323041373	27.59326323877536	21.743399680224183
33	24.683190300510173	25.320482036621677	27.604788727619926	22.391538935248224
34	22.41594585280143	26.35901897750835	29.240504183639455	21.984530986050768
35	22.96318058294115	27.09111350911587	28.53100864976638	21.4146972581766
36	23.780247346030205	27.353487872812842	27.46128509200617	21.404979689150785
37	22.805665568732028	27.973378380668816	27.967841626223876	21.25311442437528
38	23.437420550398592	27.374165955739866	27.815411386505005	21.37300210735654
39	23.06431109780281	26.485008389877905	27.53439284967712	22.916287662642162
40	24.610534522793913	25.165565907152015	28.619822710862774	21.604076859191295
41	22.111763343295728	25.11754303696631	29.42174814546975	23.348945474268216
42	22.206114158837057	25.238560669834293	28.954626862298653	23.600698309029994
43	22.435832970807745	25.392911824360592	28.94626523313691	23.224989971694757
44	22.31210345821163	25.072458036486083	29.039260108814176	23.576178396488114
45	22.296962129729547	25.435284945112684	28.28106373482336	23.98668919033441
46	22.75967660834242	25.99698303380245	28.068972141085542	23.174368216769587
47	21.337182695947433	25.772123006344668	29.99271182323064	22.897982474477256
48	21.813682563178325	25.916756591845154	29.56479980112882	22.704761043847704
49	22.797868914513643	25.478901010740174	29.216888231006955	22.50634184373923
50	21.26825575285736	25.373137701342944	30.029661184526468	23.328945361273227
51	21.755151159046097	25.572686851338144	28.72807191001079	23.94409007960497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	683.0
1	1284.5
2	1886.0
3	7753.5
4	13621.0
5	10207.0
6	6793.0
7	5426.0
8	4059.0
9	3890.5
10	3722.0
11	3556.5
12	3391.0
13	3338.0
14	3285.0
15	3187.0
16	3089.0
17	2986.5
18	2884.0
19	2969.0
20	3054.0
21	3397.0
22	3740.0
23	4230.5
24	4721.0
25	5327.0
26	7021.0
27	8109.0
28	10103.5
29	12098.0
30	13806.0
31	15514.0
32	17794.5
33	20075.0
34	23097.0
35	26119.0
36	28471.5
37	30824.0
38	33245.0
39	35666.0
40	39068.0
41	42470.0
42	45143.5
43	47817.0
44	53515.5
45	59214.0
46	60397.5
47	61581.0
48	61850.5
49	62120.0
50	58587.5
51	55055.0
52	51826.0
53	48597.0
54	47124.0
55	45651.0
56	45838.5
57	46026.0
58	44965.5
59	43905.0
60	41008.5
61	38112.0
62	35703.0
63	33294.0
64	30140.0
65	26986.0
66	23562.0
67	20138.0
68	17494.5
69	14851.0
70	12631.0
71	10411.0
72	9142.5
73	7874.0
74	6431.5
75	3932.0
76	2875.0
77	2224.0
78	1573.0
79	1281.0
80	989.0
81	755.0
82	521.0
83	417.5
84	314.0
85	236.0
86	158.0
87	133.5
88	109.0
89	81.5
90	54.0
91	42.0
92	30.0
93	19.0
94	8.0
95	6.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	884995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.9220728117902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98373242808532	26.217269503578944
2	8.246433912506346	5.924579988853377
3	3.943020794274051	4.249244402109458
4	2.6643827425115987	3.8284060349991567
5	1.9257568686419233	3.4588589226580133
6	1.3630758861377572	2.937870673790157
7	1.0141523097903915	2.550131718017496
8	0.8382163782483527	2.4088375817177923
9	0.643088557172904	2.079096657767475
>10	6.320792656156242	42.728554437033125
>50	0.037484127614107667	0.8891549578349792
>100	0.0173003273951244	1.082957901395305
>500	0.0012815057329664571	0.3965858584695149
>1k	9.611292997248428E-4	0.4994726133945719
>5k	3.203764332416143E-4	0.7489787483806161
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6508	0.7353713862790185	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1892	0.2137865185679015	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1386	0.1566110542997418	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC	1062	0.12000067796993202	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	997	0.11265600370623562	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	914	0.10327741964643869	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03141260685088616	0.0
2	0.0	0.0	0.0	0.14384262057977729	0.0
3	0.0	0.0	0.0	0.22316510262769845	0.0
4	0.0	0.0	0.0	0.3483635500765541	0.0
5	0.0	0.0	0.0	0.7578573890247967	0.0
6	0.0	0.0	0.0	1.0975203249735874	0.0
7	0.0	0.0	0.0	1.3794428217108572	0.0
8	0.0	0.0	0.0	1.8740218871293057	0.0
9	0.0	0.0	0.0	2.1518765642743745	0.0
10	0.0	0.0	0.0	2.7214843021711985	0.0
11	0.0	0.0	0.0	3.186797665523534	0.0
12	0.0	0.0	0.0	3.646574274430929	0.0
13	0.0	0.0	0.0	3.8226204667822983	0.0
14	0.0	0.0	0.0	3.9359544404205673	0.0
15	0.0	0.0	0.0	4.0567460833112055	0.0
16	0.0	0.0	0.0	4.279120221018198	0.0
17	0.0	0.0	0.0	4.517765637093995	0.0
18	2.2598997734450476E-4	0.0	0.0	4.794151379386324	0.0
19	2.2598997734450476E-4	0.0	0.0	4.98206204554828	0.0
20	2.2598997734450476E-4	0.0	0.0	5.170424691664925	0.0
21	2.2598997734450476E-4	0.0	0.0	5.409635082684083	0.0
22	2.2598997734450476E-4	0.0	0.0	5.655964157989593	0.0
23	2.2598997734450476E-4	0.0	0.0	5.8996943485556415	0.0
24	2.2598997734450476E-4	0.0	0.0	6.1117859422934595	0.0
25	2.2598997734450476E-4	0.0	0.0	6.305120367911683	0.0
26	2.2598997734450476E-4	0.0	0.0	6.48353945502517	0.0
27	2.2598997734450476E-4	0.0	0.0	6.686930434635224	0.0
28	2.2598997734450476E-4	0.0	0.0	6.872129221069046	0.0
29	2.2598997734450476E-4	0.0	0.0	7.090322544195165	0.0
30	2.2598997734450476E-4	0.0	0.0	7.409985367148967	0.0
31	2.2598997734450476E-4	0.0	0.0	7.6358623495048	0.0
32	2.2598997734450476E-4	0.0	0.0	7.856202577415692	0.0
33	2.2598997734450476E-4	0.0	0.0	8.086599359318415	0.0
34	2.2598997734450476E-4	0.0	0.0	8.332024474714546	0.0
35	2.2598997734450476E-4	0.0	0.0	8.614398951406505	0.0
36	2.2598997734450476E-4	0.0	0.0	8.870784580703846	0.0
37	2.2598997734450476E-4	0.0	0.0	9.121181475601556	0.0
38	2.2598997734450476E-4	0.0	0.0	9.42129616551506	0.0
39	2.2598997734450476E-4	0.0	0.0	9.913728326148735	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGCG	20	7.032405E-4	45.000004	10
GACGTCG	20	7.032405E-4	45.000004	19
CGACTAA	20	7.032405E-4	45.000004	10
TTCGACG	25	3.8900755E-5	45.0	1
TTACGGG	275	0.0	42.545456	3
CGAATAT	175	0.0	42.428574	14
CGTTATT	520	0.0	41.971153	1
CGTTTTT	2325	0.0	41.51613	1
CGGTCTA	50	1.0804797E-9	40.5	31
TACGAAT	190	0.0	40.263157	12
TAGTACG	45	1.9274012E-8	40.0	1
CGGTTTT	675	0.0	39.333332	1
CGTAGCG	35	6.246659E-6	38.57143	2
GTATACG	35	6.246659E-6	38.57143	1
ACGGGTA	165	0.0	38.181816	5
TACGGGA	380	0.0	37.894737	4
AATTGCG	30	1.1397097E-4	37.499996	1
CCGAACG	30	1.1397097E-4	37.499996	36
CGTTTTA	265	0.0	37.35849	1
CTACGAA	205	0.0	37.317074	11
>>END_MODULE