FastQCFastQC Report
Sat 14 Jan 2017
SRR2936120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034128
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57340.5544768152491761No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16000.15471972521776803No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.10956090541983196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG351.2117744E-745.01
TAAACGG850.042.3529432
AATACGG556.184564E-1140.909092
CGTTCAT1050.040.71428317
CGTTATT4450.038.9325831
CGCATCG356.247683E-638.57142621
TAGGCGA700.038.5714266
CGTTTTT19600.038.4566351
CCCGATC900.037.50000441
GACGGGA5000.036.8999984
TACGGGA3600.036.8750044
CATACGG552.748493E-936.818182
TCGACGG1100.036.818182
TAACGGG2850.036.315793
CGTTTTA3350.036.2686581
CGAATAT1800.036.25000414
ACACGAC1000.036.00000426
TTAGCGA250.002107010736.0000041
TATTGCG504.8781658E-836.0000041
GTTAACG250.002107010736.0000041
Produced by FastQC (version 0.11.5)