##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936120.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1034128
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900592576547584	31.0	31.0	34.0	30.0	34.0
2	32.03342526263673	33.0	31.0	34.0	30.0	34.0
3	31.852173038540684	33.0	31.0	34.0	30.0	34.0
4	35.64719067658936	37.0	35.0	37.0	33.0	37.0
5	31.161988651308157	37.0	35.0	37.0	0.0	37.0
6	33.39662885058716	37.0	35.0	37.0	17.0	37.0
7	29.474048667089566	35.0	32.0	37.0	0.0	37.0
8	32.54896009004688	35.0	33.0	37.0	17.0	37.0
9	36.39617242739777	39.0	35.0	39.0	32.0	39.0
10	36.809173525907816	38.0	35.0	39.0	33.0	39.0
11	36.97365413179026	39.0	37.0	39.0	33.0	39.0
12	37.04513561183915	39.0	37.0	39.0	33.0	39.0
13	36.914966039020314	39.0	37.0	39.0	33.0	39.0
14	38.208392964894095	40.0	37.0	41.0	33.0	41.0
15	38.2850285457893	40.0	38.0	41.0	34.0	41.0
16	38.31092766079247	40.0	37.0	41.0	34.0	41.0
17	38.269719028979004	40.0	37.0	41.0	34.0	41.0
18	38.17260145745981	40.0	37.0	41.0	33.0	41.0
19	38.085929401389386	40.0	37.0	41.0	34.0	41.0
20	37.980873740968235	40.0	37.0	41.0	33.0	41.0
21	37.87536939334396	40.0	36.0	41.0	33.0	41.0
22	37.82088387511024	40.0	36.0	41.0	33.0	41.0
23	37.775893313013476	40.0	36.0	41.0	33.0	41.0
24	37.727817059396905	40.0	36.0	41.0	33.0	41.0
25	37.57491625794873	40.0	36.0	41.0	33.0	41.0
26	37.41194997137685	39.0	36.0	41.0	32.0	41.0
27	37.36324613587486	39.0	36.0	41.0	32.0	41.0
28	37.19637124224467	39.0	35.0	41.0	32.0	41.0
29	37.16591949932697	39.0	35.0	41.0	32.0	41.0
30	37.061742840344714	39.0	35.0	41.0	31.0	41.0
31	36.93121837915616	39.0	35.0	41.0	31.0	41.0
32	36.84771324246128	39.0	35.0	41.0	31.0	41.0
33	36.675921162564016	39.0	35.0	41.0	30.0	41.0
34	36.4890226354958	39.0	35.0	41.0	30.0	41.0
35	36.369930995002555	39.0	35.0	41.0	30.0	41.0
36	36.22519552705275	39.0	35.0	40.0	29.0	41.0
37	36.07316212306407	39.0	35.0	40.0	29.0	41.0
38	35.96083560255597	39.0	35.0	40.0	28.0	41.0
39	35.9200553509817	39.0	35.0	40.0	28.0	41.0
40	35.73761468599632	39.0	35.0	40.0	27.0	41.0
41	35.61727078272709	38.0	35.0	40.0	27.0	41.0
42	35.53968560951836	38.0	35.0	40.0	26.0	41.0
43	35.37478822737611	38.0	34.0	40.0	26.0	41.0
44	35.12000835486516	38.0	34.0	40.0	24.0	41.0
45	35.013923808271315	38.0	34.0	40.0	24.0	41.0
46	34.894579781226305	38.0	34.0	40.0	24.0	41.0
47	34.780281551219964	38.0	34.0	40.0	23.0	41.0
48	34.686327031083195	38.0	34.0	40.0	23.0	41.0
49	34.49520755651138	38.0	33.0	40.0	23.0	41.0
50	34.3269807992821	37.0	33.0	40.0	23.0	41.0
51	32.352858640323056	35.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	23.0
9	33.0
10	72.0
11	46.0
12	39.0
13	36.0
14	46.0
15	74.0
16	152.0
17	257.0
18	528.0
19	969.0
20	1593.0
21	2474.0
22	3586.0
23	5157.0
24	7028.0
25	9891.0
26	12617.0
27	14963.0
28	16493.0
29	18962.0
30	22795.0
31	28320.0
32	36329.0
33	49166.0
34	70589.0
35	86031.0
36	103101.0
37	159682.0
38	196982.0
39	186063.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4759101387836	20.39109278541921	23.624251543329258	14.508745532467934
2	32.662784490894744	24.956001578141198	24.727596583788465	17.65361734717559
3	32.309540018256925	23.925858307675647	27.000139247752696	16.76446242631473
4	29.06013568919902	25.654947936812466	26.425258768740427	18.859657605248092
5	21.705920350285457	39.56686212925286	21.762683149474725	16.964534370986957
6	25.96709498243931	35.587567496480126	24.481882320176997	13.963455200903564
7	67.66222363189083	20.139963331425122	7.996108798910773	4.201704237773274
8	81.9478826605604	5.564398217628765	8.626204879860133	3.8615142419507067
9	75.1750266891526	8.066602973713119	11.02900221249207	5.72936812464221
10	39.73618352853806	27.40057323658193	18.34511781907075	14.51812541580926
11	29.110903099036094	26.64031918679313	25.369876843098726	18.87890087107205
12	28.14844970835332	23.197708600869525	29.321031825847477	19.33280986492968
13	24.146043816626182	23.917638822273453	30.59901675614624	21.337300604954127
14	19.6529829963022	27.164625655624835	30.148105456964707	23.034285891108258
15	20.05486748255535	26.8708515773676	32.55892887534232	20.51535206473473
16	22.370344870267513	25.711033837203907	30.980110779323255	20.938510513205326
17	22.42643077065895	25.45806708647285	29.314262837869197	22.801239304998994
18	22.458148314328593	26.749686692556434	29.252858446923398	21.539306546191575
19	24.416029737131186	27.5605147525258	27.0580624448811	20.965393065461914
20	25.653110640075504	26.188344189500718	27.940158278278897	20.218386892144878
21	25.245714263611468	26.742821004749896	27.979805207865947	20.031659523772685
22	23.144910494638964	25.68521498305819	27.94489656986369	23.224977952439158
23	23.435590178391845	26.32575464545975	28.234996054647006	22.0036591215014
24	22.23380471276283	25.137797255272076	30.047344235916633	22.581053796048458
25	23.099364875527982	26.639062089025728	27.68138953785218	22.58018349759411
26	21.766164343292125	28.0033032661334	27.729352652669686	22.501179737904785
27	22.733936224529266	27.260841984744633	28.523258242693363	21.48196354803274
28	21.214298423406	27.43557857441245	29.495865115343555	21.854257886837996
29	21.987316850525275	26.776956044126067	28.14061702226417	23.095110083084492
30	22.488802159887364	26.630455804310493	28.21285179397521	22.667890241826928
31	22.86409419336871	27.38403756594928	27.03727198180496	22.714596258877044
32	22.938843160614546	27.039882877168008	27.20968777559451	22.811586186622932
33	22.8822737610818	26.77279795151084	27.176036235359646	23.168892052047717
34	21.41175947271518	27.13242461281389	27.84326505036127	23.612550864109664
35	22.41047529899587	25.900468800767413	27.37417418346665	24.31488171677007
36	21.865571766744544	28.326377392353752	26.54961474788421	23.258436093017497
37	22.281864527408597	28.379755697553882	27.287627837172963	22.050751937864558
38	21.19234756239073	26.99685145359182	27.59648708863893	24.214313895378524
39	22.79147262234462	25.559408506490495	27.38113657110145	24.267982300063434
40	22.8700895827209	25.96351708879365	28.785701576593997	22.380691751891447
41	20.246043043027555	26.78691612643696	28.918083641483456	24.048957189052032
42	21.116728296690546	27.420300001547197	27.44176736342116	24.021204338341096
43	21.9949561369579	25.896310708152182	28.19689632231213	23.911836832577784
44	21.86905296056194	26.520411399749356	27.511584639425678	24.098951000263025
45	21.557196014419876	26.04551854315907	27.399993037612365	24.99729240480869
46	21.19080036513855	27.73573484133492	26.917074095276405	24.156390698250117
47	21.5121338944502	26.111854625346187	29.00337289000975	23.372638590193866
48	21.058418300249098	25.43495582751845	29.32402952052357	24.18259635170888
49	20.642222239413304	26.176643510281128	28.78434777899834	24.396786471307227
50	20.077205142883667	25.755612458032275	29.73335989355283	24.43382250553123
51	20.476188634288985	25.125516377082914	28.41379403710179	25.984500951526314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	629.0
1	1283.5
2	1938.0
3	7166.5
4	12395.0
5	9218.5
6	6042.0
7	4736.0
8	3430.0
9	3220.0
10	3010.0
11	2898.0
12	2786.0
13	2800.0
14	2814.0
15	2803.5
16	2793.0
17	2815.5
18	2838.0
19	2942.0
20	3046.0
21	3762.5
22	4479.0
23	4756.5
24	5034.0
25	6225.5
26	8912.5
27	10408.0
28	12847.0
29	15286.0
30	17980.0
31	20674.0
32	23625.5
33	26577.0
34	29091.0
35	31605.0
36	34551.5
37	37498.0
38	39363.5
39	41229.0
40	44974.0
41	48719.0
42	50563.5
43	52408.0
44	56327.5
45	60247.0
46	63459.5
47	66672.0
48	72590.5
49	78509.0
50	79659.0
51	80809.0
52	78004.5
53	75200.0
54	69151.5
55	63103.0
56	59427.0
57	55751.0
58	52661.0
59	49571.0
60	48132.5
61	46694.0
62	42482.0
63	38270.0
64	32369.0
65	26468.0
66	21817.5
67	17167.0
68	14586.0
69	12005.0
70	10030.0
71	8055.0
72	6626.0
73	5197.0
74	4127.5
75	2424.0
76	1790.0
77	1457.0
78	1124.0
79	869.0
80	614.0
81	469.0
82	324.0
83	274.0
84	224.0
85	153.0
86	82.0
87	63.0
88	44.0
89	40.0
90	36.0
91	33.0
92	30.0
93	21.5
94	13.0
95	12.0
96	11.0
97	7.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1034128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.73752011150352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.95096323515563	25.71348995658425
2	8.863306312652028	6.33505175205636
3	4.377007064911745	4.6927013403142945
4	2.774980225557513	3.966836464795448
5	1.9197872339719122	3.430421744193947
6	1.3677346593881696	2.932766693825108
7	1.0461039642514167	2.6169613022810996
8	0.8163856122912618	2.3340477790400866
9	0.6689737256059142	2.151671577561792
>10	6.120730890074844	41.13547850076953
>50	0.06587427161433282	1.5336296950692785
>100	0.024599537938342325	1.640547694925288
>500	0.0027332819898861767	0.6893328416325594
>1k	5.466563979772353E-4	0.2669614079894834
>5k	2.7332819898861767E-4	0.5601012489614703
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5734	0.5544768152491761	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1600	0.15471972521776803	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1133	0.10956090541983196	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08238825367846146	0.0
2	0.0	0.0	0.0	0.3021869633159531	0.0
3	0.0	0.0	0.0	0.478857549548992	0.0
4	0.0	0.0	0.0	0.6498228459146257	0.0
5	0.0	0.0	0.0	1.1771270094224313	0.0
6	0.0	0.0	0.0	1.7816943357108597	0.0
7	0.0	0.0	0.0	2.2206148561880155	0.0
8	0.0	0.0	0.0	2.9490546624789196	0.0
9	0.0	0.0	0.0	3.3147734129624187	0.0
10	0.0	0.0	0.0	3.9475770891030897	0.0
11	0.0	0.0	0.0	4.67224560209181	0.0
12	0.0	0.0	0.0	5.266852846069345	0.0
13	0.0	0.0	0.0	5.552020639611344	0.0
14	0.0	0.0	0.0	5.695039685609518	0.0
15	0.0	0.0	0.0	5.889212940757817	0.0
16	0.0	0.0	0.0	6.249516500858695	0.0
17	0.0	0.0	0.0	6.659330373029258	0.0
18	9.669982826110501E-5	0.0	0.0	7.165167174663098	0.0
19	9.669982826110501E-5	0.0	0.0	7.445209877307258	0.0
20	9.669982826110501E-5	0.0	0.0	7.744882645088422	0.0
21	9.669982826110501E-5	0.0	0.0	8.092325128030573	0.0
22	9.669982826110501E-5	0.0	0.0	8.47013135704671	0.0
23	1.9339965652221002E-4	0.0	0.0	8.860121764423747	0.0
24	1.9339965652221002E-4	0.0	0.0	9.175943403524515	0.0
25	1.9339965652221002E-4	0.0	0.0	9.46159469620782	0.0
26	1.9339965652221002E-4	0.0	0.0	9.728099422895426	0.0
27	1.9339965652221002E-4	0.0	0.0	9.989575758513453	0.0
28	1.9339965652221002E-4	0.0	0.0	10.263429672148902	0.0
29	2.9009948478331504E-4	0.0	0.0	10.551788560023518	0.0
30	2.9009948478331504E-4	0.0	0.0	10.897297046400446	0.0
31	2.9009948478331504E-4	0.0	0.0	11.193295220707688	0.0
32	3.8679931304442004E-4	0.0	0.0	11.50331487011279	0.0
33	3.8679931304442004E-4	0.0	0.0	11.798442745965684	0.0
34	3.8679931304442004E-4	0.0	0.0	12.083997338820726	0.0
35	3.8679931304442004E-4	0.0	0.0	12.401462875001934	0.0
36	3.8679931304442004E-4	0.0	0.0	12.716704315133136	0.0
37	3.8679931304442004E-4	0.0	0.0	13.018311079479522	0.0
38	3.8679931304442004E-4	0.0	0.0	13.318950845543299	0.0
39	3.8679931304442004E-4	0.0	0.0	13.625102501817956	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	35	1.2117744E-7	45.0	1
TAAACGG	85	0.0	42.352943	2
AATACGG	55	6.184564E-11	40.90909	2
CGTTCAT	105	0.0	40.714283	17
CGTTATT	445	0.0	38.932583	1
CGCATCG	35	6.247683E-6	38.571426	21
TAGGCGA	70	0.0	38.571426	6
CGTTTTT	1960	0.0	38.456635	1
CCCGATC	90	0.0	37.500004	41
GACGGGA	500	0.0	36.899998	4
TACGGGA	360	0.0	36.875004	4
CATACGG	55	2.748493E-9	36.81818	2
TCGACGG	110	0.0	36.81818	2
TAACGGG	285	0.0	36.31579	3
CGTTTTA	335	0.0	36.268658	1
CGAATAT	180	0.0	36.250004	14
ACACGAC	100	0.0	36.000004	26
TTAGCGA	25	0.0021070107	36.000004	1
TATTGCG	50	4.8781658E-8	36.000004	1
GTTAACG	25	0.0021070107	36.000004	1
>>END_MODULE