##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936119.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444015
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00901320901321	33.0	31.0	34.0	30.0	34.0
2	32.15233043928696	33.0	31.0	34.0	30.0	34.0
3	32.07494341407385	33.0	31.0	34.0	30.0	34.0
4	35.80012386968909	37.0	35.0	37.0	35.0	37.0
5	31.33528146571625	37.0	35.0	37.0	0.0	37.0
6	33.52678175286871	37.0	35.0	37.0	17.0	37.0
7	29.37927097057532	35.0	32.0	37.0	0.0	37.0
8	32.49969708230578	35.0	33.0	37.0	17.0	37.0
9	36.43224891050978	39.0	35.0	39.0	32.0	39.0
10	36.89984347375652	38.0	37.0	39.0	34.0	39.0
11	37.07722712070538	39.0	37.0	39.0	34.0	39.0
12	37.08536423319032	39.0	37.0	39.0	34.0	39.0
13	36.904273504273505	39.0	37.0	39.0	33.0	39.0
14	38.1886873191221	40.0	37.0	41.0	33.0	41.0
15	38.27624066754502	40.0	37.0	41.0	34.0	41.0
16	38.32448002882786	40.0	37.0	41.0	34.0	41.0
17	38.27607175433263	40.0	37.0	41.0	34.0	41.0
18	38.2161368422238	40.0	37.0	41.0	34.0	41.0
19	38.12872312872313	40.0	37.0	41.0	34.0	41.0
20	38.067445919619836	40.0	37.0	41.0	34.0	41.0
21	37.97925070098983	40.0	37.0	41.0	33.0	41.0
22	37.939824105041495	40.0	36.0	41.0	33.0	41.0
23	37.917374413026586	40.0	36.0	41.0	33.0	41.0
24	37.86370280283324	40.0	36.0	41.0	33.0	41.0
25	37.76168823125345	40.0	36.0	41.0	33.0	41.0
26	37.587191874148395	40.0	36.0	41.0	33.0	41.0
27	37.53756292017162	40.0	36.0	41.0	33.0	41.0
28	37.43080526558787	39.0	36.0	41.0	33.0	41.0
29	37.39554294336903	39.0	36.0	41.0	33.0	41.0
30	37.262412305890564	39.0	36.0	41.0	32.0	41.0
31	37.08002207132642	39.0	35.0	41.0	32.0	41.0
32	37.00885105232931	39.0	35.0	41.0	31.0	41.0
33	36.843766539418716	39.0	35.0	41.0	31.0	41.0
34	36.5882211186559	39.0	35.0	41.0	30.0	41.0
35	36.53422744727092	39.0	35.0	41.0	30.0	41.0
36	36.36349222436179	39.0	35.0	41.0	30.0	41.0
37	36.25093296397644	39.0	35.0	40.0	30.0	41.0
38	36.203855725594856	39.0	35.0	40.0	30.0	41.0
39	36.10092677049199	39.0	35.0	40.0	29.0	41.0
40	35.894312129094736	39.0	35.0	40.0	28.0	41.0
41	35.71567627219801	38.0	35.0	40.0	27.0	41.0
42	35.714068218416045	38.0	35.0	40.0	27.0	41.0
43	35.60731506818463	38.0	35.0	40.0	27.0	41.0
44	35.48541603324212	38.0	34.0	40.0	27.0	41.0
45	35.345477067216194	38.0	34.0	40.0	26.0	41.0
46	35.19322770627119	38.0	34.0	40.0	26.0	41.0
47	35.01298829994482	38.0	34.0	40.0	25.0	41.0
48	34.90163620598403	38.0	34.0	40.0	24.0	41.0
49	34.760474308300395	38.0	34.0	40.0	24.0	41.0
50	34.58374604461561	37.0	34.0	40.0	24.0	41.0
51	32.61993851559069	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	22.0
10	26.0
11	15.0
12	16.0
13	16.0
14	14.0
15	25.0
16	47.0
17	96.0
18	206.0
19	295.0
20	563.0
21	867.0
22	1239.0
23	1862.0
24	2595.0
25	3535.0
26	4429.0
27	5273.0
28	6269.0
29	7559.0
30	9050.0
31	11627.0
32	15409.0
33	21997.0
34	32012.0
35	38636.0
36	45469.0
37	69596.0
38	84940.0
39	80289.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34673378151639	19.903832077745122	24.323052149139105	12.426381991599383
2	32.21377656160265	26.16060268234181	24.85434050651442	16.771280249541118
3	33.395042960260355	24.645113340765516	25.9389885476842	16.020855151289933
4	30.051462225375268	25.336531423487944	25.849802371541504	18.762203979595284
5	22.7357183878923	38.91873022307805	21.204238595542943	17.14131279348671
6	26.296183687488035	35.9713072756551	23.67014627884193	14.062362758014931
7	67.91572356789747	20.729705077531165	7.328581241624719	4.0259901129466344
8	81.84430706169836	6.593921376530072	7.592761505804985	3.969010055966578
9	75.09205770075336	9.013884666058578	9.659583572627051	6.234474060561017
10	40.632185849577155	28.099275925362882	17.05212661734401	14.216411607715957
11	32.194407846581754	26.55698568742047	23.846716890195154	17.40188957580262
12	30.716980282197675	23.695821087125434	26.84931815366598	18.73788047701091
13	25.01379457901197	25.11379120074772	28.628086888956457	21.244327331283852
14	20.317556839295968	29.19608571782485	27.907840951319212	22.57851649155997
15	20.056529621747014	27.670461583505062	33.01870432305215	19.254304471695775
16	23.08660743443352	25.654088262783915	31.257502561850387	20.001801740932176
17	22.461853766201592	25.625485625485627	28.990687251556817	22.921973356755963
18	23.200567548393636	27.624066754501538	27.9312635834375	21.24410211366733
19	25.15478080695472	28.37156402373794	26.409242930982064	20.064412238325282
20	27.04976183237053	26.961476526693918	27.488936184588358	18.499825456347196
21	26.154972241928764	27.223629832325486	27.144353231309754	19.477044694436
22	23.24448498361542	26.198889677150543	27.040077474860087	23.51654786437395
23	23.86743691091517	27.88126527256962	27.276330754591626	20.974967061923582
24	23.529160050899183	25.317162708467055	29.046766438070787	22.106910802562975
25	22.56793126358344	27.285564676869022	26.817787687352908	23.328716372194634
26	22.134387351778656	28.422463205071903	27.5283492674797	21.91480017566974
27	22.359154533067578	28.966814184205486	27.380156075808248	21.293875206918685
28	21.31639696857088	28.787315743837482	28.360303142911842	21.535984144679794
29	22.546760807630374	27.648165039469387	28.289810028940465	21.515264123959778
30	23.51407047059221	26.647973604495345	27.856040899519158	21.98191502539329
31	24.442417485895746	27.23826897739941	25.991239034717296	22.328074501987548
32	26.00114860984426	27.942299246647075	25.64350303480738	20.413049108701284
33	25.341936646284474	27.348400391878652	25.967591184982492	21.342071776854386
34	23.61046361046361	26.658558832471872	28.737993085819173	20.99298447124534
35	23.920588268414356	25.873450221276308	27.3560577908404	22.849903719468937
36	23.867662128531695	28.898798464015858	26.30158891028456	20.93195049716789
37	23.887706496402146	28.524486785356352	27.2299359255881	20.3578707926534
38	24.703669921061223	27.77113385809038	25.590576894924723	21.934619325923673
39	25.133835568618178	25.98966251140164	27.313041226084707	21.563460693895475
40	25.107935542718153	25.13788948571557	29.217030956161388	20.537144015404884
41	21.103566320957626	27.392317827100438	28.82109838631578	22.68301746562616
42	22.38122585948673	27.75469297208428	27.116201029244507	22.74788013918449
43	23.610238392847087	26.518023039762166	26.78152765109287	23.090210916297874
44	23.188631014717973	27.163721946330643	27.213945474815038	22.433701564136346
45	23.52893483328266	26.04101212796865	26.05970519013997	24.370347848608716
46	22.674008760965283	28.48687544339718	26.48694300868214	22.352172786955396
47	22.682116595160075	26.67162145423015	28.92357240183327	21.722689548776504
48	22.722205330900984	25.975023366327715	28.560972039232908	22.741799263538397
49	21.97425762643154	25.997545127979908	28.928752407013274	23.099444838575273
50	20.760334673378154	26.556310034570906	29.492021665934708	23.191333626116233
51	21.51909282344065	25.71692397779354	27.64546242807112	25.118520770694687
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	382.0
1	788.0
2	1194.0
3	1902.0
4	2610.0
5	1962.0
6	1314.0
7	1101.0
8	888.0
9	892.0
10	896.0
11	907.0
12	918.0
13	988.0
14	1058.0
15	1136.0
16	1214.0
17	1302.5
18	1391.0
19	1480.0
20	1569.0
21	1940.0
22	2311.0
23	2517.5
24	2724.0
25	3228.0
26	4394.5
27	5057.0
28	6365.5
29	7674.0
30	9001.0
31	10328.0
32	11204.5
33	12081.0
34	13276.0
35	14471.0
36	14943.5
37	15416.0
38	16420.5
39	17425.0
40	19117.0
41	20809.0
42	22175.5
43	23542.0
44	25047.0
45	26552.0
46	27140.5
47	27729.0
48	30487.0
49	33245.0
50	33250.0
51	33255.0
52	32064.5
53	30874.0
54	29162.0
55	27450.0
56	25662.0
57	23874.0
58	23231.5
59	22589.0
60	21871.5
61	21154.0
62	19145.5
63	17137.0
64	14276.0
65	11415.0
66	9198.5
67	6982.0
68	5949.0
69	4916.0
70	3937.5
71	2959.0
72	2518.5
73	2078.0
74	1669.0
75	958.0
76	656.0
77	532.5
78	409.0
79	312.0
80	215.0
81	154.5
82	94.0
83	80.5
84	67.0
85	53.0
86	39.0
87	31.0
88	23.0
89	21.5
90	20.0
91	15.0
92	10.0
93	6.5
94	3.0
95	4.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	444015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.08466485379657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9685815272404	27.02814642614289
2	9.14667148790795	6.966958362895021
3	4.663678391104355	5.328438855333077
4	3.0340201053538878	4.621985554883335
5	2.0451007065546656	3.89434875006985
6	1.5127199700503433	3.4566859846207505
7	1.0631011554246599	2.834149584702245
8	0.85544654721232	2.606351604073494
9	0.7434565199662999	2.5482863156657802
>10	5.89871905271151	37.351773215284126
>50	0.046063395215005465	1.2277913667477662
>100	0.02066947221186142	1.6389294173824822
>500	0.0017716690467309787	0.4961545621992254
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	978	0.22026282895848112	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	626	0.14098622794274968	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGC	602	0.13558100514622254	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	490	0.11035663209576253	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2521761652196434E-4	0.0	0.0	0.07815051293312163	0.0
2	2.2521761652196434E-4	0.0	0.0	0.4148508496334583	0.0
3	2.2521761652196434E-4	0.0	0.0	0.5522335957118566	0.0
4	2.2521761652196434E-4	0.0	0.0	0.7438937873720483	0.0
5	2.2521761652196434E-4	0.0	0.0	1.4044570566309698	0.0
6	2.2521761652196434E-4	0.0	0.0	1.7666069839982883	0.0
7	2.2521761652196434E-4	0.0	0.0	2.1107395020438497	0.0
8	2.2521761652196434E-4	0.0	0.0	2.6494600407643887	0.0
9	2.2521761652196434E-4	0.0	0.0	2.8647680821593866	0.0
10	2.2521761652196434E-4	0.0	0.0	3.635012330664505	0.0
11	2.2521761652196434E-4	0.0	0.0	4.428453993671385	0.0
12	2.2521761652196434E-4	0.0	0.0	5.261759174802653	0.0
13	2.2521761652196434E-4	0.0	0.0	5.531119444162923	0.0
14	2.2521761652196434E-4	0.0	0.0	5.649583910453476	0.0
15	2.2521761652196434E-4	0.0	0.0	5.890791977748499	0.0
16	2.2521761652196434E-4	0.0	0.0	6.311948920644573	0.0
17	2.2521761652196434E-4	0.0	0.0	6.7542763194937105	0.0
18	2.2521761652196434E-4	0.0	0.0	7.236016801234192	0.0
19	2.2521761652196434E-4	0.0	0.0	7.529700573178834	0.0
20	2.2521761652196434E-4	0.0	0.0	7.801763453937367	0.0
21	2.2521761652196434E-4	0.0	0.0	8.159634246590768	0.0
22	2.2521761652196434E-4	0.0	0.0	8.524036350123307	0.0
23	2.2521761652196434E-4	0.0	0.0	8.896771505467157	0.0
24	2.2521761652196434E-4	0.0	0.0	9.225138790356182	0.0
25	2.2521761652196434E-4	0.0	0.0	9.492922536400798	0.0
26	2.2521761652196434E-4	0.0	0.0	9.74651757260453	0.0
27	2.2521761652196434E-4	0.0	0.0	9.99763521502652	0.0
28	2.2521761652196434E-4	0.0	0.0	10.275778971431146	0.0
29	2.2521761652196434E-4	0.0	0.0	10.558877515399255	0.0
30	2.2521761652196434E-4	0.0	0.0	10.857741292523901	0.0
31	2.2521761652196434E-4	0.0	0.0	11.165163339076383	0.0
32	2.2521761652196434E-4	0.0	0.0	11.474387126561039	0.0
33	2.2521761652196434E-4	0.0	0.0	11.746675224936094	0.0
34	2.2521761652196434E-4	0.0	0.0	12.024143328491155	0.0
35	2.2521761652196434E-4	0.0	0.0	12.329988851727983	0.0
36	2.2521761652196434E-4	0.0	0.0	12.63245611071698	0.0
37	2.2521761652196434E-4	0.0	0.0	12.955868608042522	0.0
38	2.2521761652196434E-4	0.0	0.0	13.259461955114128	0.0
39	2.2521761652196434E-4	0.0	0.0	13.567559654516176	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTACA	20	7.0286036E-4	45.000004	34
CGGTCTA	20	7.0286036E-4	45.000004	31
CGTCATT	20	7.0286036E-4	45.000004	28
TATAACG	20	7.0286036E-4	45.000004	1
TAAGACG	20	7.0286036E-4	45.000004	1
ACGGTCT	20	7.0286036E-4	45.000004	30
GCGTACG	35	1.2096825E-7	45.0	1
GCGTTCC	30	2.162482E-6	44.999996	23
ACGTATG	30	2.162482E-6	44.999996	1
CGGTAGG	30	2.162482E-6	44.999996	31
TACGAAT	55	6.002665E-11	40.909092	12
CGGGACC	50	1.0786607E-9	40.5	6
GCGATAT	45	1.9237632E-8	40.0	9
GCACGGG	220	0.0	38.863636	3
TACGCGG	35	6.2396302E-6	38.571426	2
TATAGCG	35	6.2396302E-6	38.571426	1
TATACGG	35	6.2396302E-6	38.571426	2
AGTACGG	35	6.2396302E-6	38.571426	2
CGTTATT	65	9.094947E-12	38.076927	1
TACGGGA	185	0.0	37.7027	4
>>END_MODULE