##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936118.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465516
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87380240421382	31.0	31.0	34.0	30.0	34.0
2	32.00082274293472	33.0	31.0	34.0	30.0	34.0
3	31.94502874229887	33.0	31.0	34.0	30.0	34.0
4	35.67214875535964	37.0	35.0	37.0	35.0	37.0
5	31.039139363630895	37.0	35.0	37.0	0.0	37.0
6	33.311426460100186	37.0	35.0	37.0	17.0	37.0
7	29.61809690751768	35.0	32.0	37.0	0.0	37.0
8	32.59125142852233	35.0	33.0	37.0	17.0	37.0
9	36.43309359936071	39.0	35.0	39.0	32.0	39.0
10	36.810994681171	38.0	35.0	39.0	33.0	39.0
11	36.87189913987919	39.0	37.0	39.0	33.0	39.0
12	36.94224688302872	39.0	37.0	39.0	33.0	39.0
13	36.76496618805798	39.0	35.0	39.0	33.0	39.0
14	38.06311705720104	40.0	37.0	41.0	33.0	41.0
15	38.128723824745016	40.0	37.0	41.0	33.0	41.0
16	38.192008437948424	40.0	37.0	41.0	33.0	41.0
17	38.094342621950695	40.0	37.0	41.0	33.0	41.0
18	38.00949484013439	40.0	37.0	41.0	33.0	41.0
19	37.902692925699654	40.0	37.0	41.0	33.0	41.0
20	37.91488155079524	40.0	37.0	41.0	33.0	41.0
21	37.85822184414714	40.0	36.0	41.0	33.0	41.0
22	37.89354823464714	40.0	36.0	41.0	33.0	41.0
23	37.85394272162504	40.0	36.0	41.0	33.0	41.0
24	37.810101049158355	40.0	36.0	41.0	33.0	41.0
25	37.719719622956035	39.0	36.0	41.0	33.0	41.0
26	37.478922314163206	39.0	36.0	41.0	33.0	41.0
27	37.45130994423393	39.0	36.0	41.0	33.0	41.0
28	37.30862956375291	39.0	36.0	41.0	32.0	41.0
29	37.215135462583454	39.0	36.0	41.0	32.0	41.0
30	36.96767458046555	39.0	35.0	41.0	31.0	41.0
31	36.880644274310654	39.0	35.0	41.0	31.0	41.0
32	36.7196766598785	39.0	35.0	41.0	30.0	41.0
33	36.523444951408756	39.0	35.0	41.0	30.0	41.0
34	36.37970123475885	39.0	35.0	41.0	30.0	41.0
35	35.99610110071405	39.0	35.0	41.0	27.0	41.0
36	35.93091537132988	39.0	35.0	40.0	27.0	41.0
37	35.76043573153232	39.0	35.0	40.0	27.0	41.0
38	35.54922924238909	39.0	35.0	40.0	25.0	41.0
39	35.59049098205003	39.0	35.0	40.0	25.0	41.0
40	35.35206093882917	38.0	35.0	40.0	24.0	41.0
41	35.26600159822648	38.0	34.0	40.0	24.0	41.0
42	35.33578222875261	38.0	34.0	40.0	24.0	41.0
43	35.0465891612748	38.0	34.0	40.0	24.0	41.0
44	34.96169412007321	38.0	34.0	40.0	23.0	41.0
45	34.764059667122076	38.0	34.0	40.0	23.0	41.0
46	34.52565325359386	38.0	33.0	40.0	22.0	41.0
47	34.378498268587975	38.0	33.0	40.0	22.0	41.0
48	34.21059641344229	38.0	33.0	40.0	21.0	41.0
49	34.08892712602789	38.0	33.0	40.0	20.0	41.0
50	33.96548990797309	38.0	33.0	40.0	20.0	41.0
51	31.633542133890135	35.0	28.0	39.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	16.0
10	18.0
11	16.0
12	10.0
13	8.0
14	5.0
15	28.0
16	42.0
17	89.0
18	176.0
19	352.0
20	608.0
21	1036.0
22	1851.0
23	2887.0
24	4036.0
25	5193.0
26	6257.0
27	7368.0
28	8292.0
29	9581.0
30	11285.0
31	13562.0
32	17070.0
33	22842.0
34	31589.0
35	38038.0
36	43652.0
37	68046.0
38	86524.0
39	85027.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.781919418451785	19.7127488636266	24.793347597075073	10.711984120846544
2	30.01873190180359	28.3221199700977	25.13297072495897	16.52617740313974
3	30.155998934515676	27.968533842016168	26.03025459919745	15.845212624270703
4	27.288428324697755	25.57441634659174	29.72013851296196	17.417016815748546
5	23.85245619914246	38.35722080444066	20.72324044715971	17.06708254925717
6	24.90118492167831	36.93686146126019	24.656725010525953	13.505228606535544
7	68.41118242981982	18.61053110956444	9.106883544282043	3.871402916333703
8	81.69407711013156	7.119626393077789	7.294915749404961	3.8913807473856967
9	75.86248378143823	7.252597118036759	8.47017073527011	8.4147483652549
10	39.167934077453836	31.672380755978313	16.03725758083503	13.12242758573282
11	27.642444083554597	24.85736258259652	29.039388549480577	18.460804784368314
12	27.436436126792636	22.373667070519595	30.66983734178847	19.520059460899304
13	22.082807035633575	24.75940676582545	34.11805394444015	19.039732254100823
14	19.348851596937592	30.20196942747403	28.767217453320615	21.681961522267763
15	17.948899715584428	27.16748726144751	37.23889189630432	17.644721126663747
16	20.593277137627922	27.22956890848005	31.740047603089906	20.43710635080212
17	20.51272136725698	24.915147921875942	31.309557566227586	23.262573144639497
18	21.125160037463804	26.226810678902552	31.166275702661135	21.481753580972512
19	22.360348516484933	28.723395114238826	28.15606767543973	20.76018869383652
20	24.845118105500134	27.21796887754664	29.435078493542647	18.501834523410583
21	22.929394478385277	28.544453896321503	29.618101203825432	18.908050421467788
22	21.03751535930022	25.432853006126532	28.792565669064007	24.73706596550924
23	20.652781000008595	29.210166782666974	29.278263260553878	20.858788956770553
24	22.385052286065353	25.22211911083615	28.814476838604904	23.578351764493593
25	19.346703443061035	30.306154890487115	28.294193969702437	22.052947696749413
26	20.60681050705024	29.106625765816858	28.760128545527973	21.526435181604928
27	21.022048651388996	29.010603287534693	28.860017700787942	21.10733036028837
28	18.682064633653837	27.10519079902732	32.09750040814924	22.11524415916961
29	23.772974505709794	25.13898555581333	29.69629400493216	21.391745933544712
30	22.203748098883818	26.12305484666478	30.638689110578373	21.034507943873034
31	22.273777915259625	26.953101504566973	27.048694352073827	23.72442622809957
32	23.98478247793846	28.37152750925854	28.176260321879376	19.46742969092362
33	23.215528574742866	25.77140205707215	28.85658065458545	22.156488713599533
34	22.69739385971696	25.991587829419398	30.30679933665008	21.00421897421356
35	20.66481066171732	26.217788432621003	30.365229122092472	22.7521717835692
36	22.084310743347167	25.824891088598456	30.663607695546446	21.427190472507927
37	20.687366277421184	26.654937746500657	33.020132498131105	19.63756347794705
38	21.172204607360435	26.52175220615403	28.788054545923238	23.5179886405623
39	22.072925527801406	24.827503243712354	32.57847206111068	20.521099167375557
40	23.041742926129285	25.743261241289233	31.584736077814725	19.630259754766755
41	19.242088349272635	27.230857800805985	32.016944637778295	21.51010921214308
42	21.15394529940969	26.157854939465025	31.825329311989275	20.86287044913601
43	21.956280772304282	25.732520471906444	30.14654705745882	22.164651698330452
44	21.711391230376613	25.797824349753824	29.471597109444144	23.01918731042542
45	20.972855927615807	24.459524484657884	29.655264265889898	24.91235532183641
46	23.284913944955704	26.78253808676823	28.26025313845281	21.67229482982325
47	19.60770413906289	25.43586042155372	33.26459240928346	21.691843030099932
48	21.213019531015046	25.15896338686533	30.267273305321407	23.36074377679822
49	19.4704371063508	23.087928234475292	34.14984662181321	23.291788037360693
50	20.111231407728198	24.12441247991476	31.600632416501256	24.163723695855783
51	19.713178494401912	24.3168870672544	29.87136854587168	26.09856589247201
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	326.0
1	557.5
2	789.0
3	1138.0
4	1487.0
5	1236.0
6	985.0
7	831.0
8	677.0
9	731.0
10	785.0
11	807.0
12	829.0
13	871.5
14	914.0
15	968.0
16	1022.0
17	1101.5
18	1181.0
19	1276.0
20	1371.0
21	1441.0
22	1511.0
23	1847.5
24	2184.0
25	3092.5
26	5013.5
27	6026.0
28	6789.5
29	7553.0
30	8639.0
31	9725.0
32	11281.0
33	12837.0
34	14380.5
35	15924.0
36	17481.0
37	19038.0
38	20707.5
39	22377.0
40	25094.0
41	27811.0
42	33537.5
43	39264.0
44	45914.5
45	52565.0
46	48774.5
47	44984.0
48	42483.0
49	39982.0
50	35642.5
51	31303.0
52	28026.0
53	24749.0
54	22720.5
55	20692.0
56	18657.5
57	16623.0
58	16279.0
59	15935.0
60	14837.0
61	13739.0
62	12147.0
63	10555.0
64	8526.0
65	6497.0
66	5163.5
67	3830.0
68	2998.0
69	2166.0
70	1666.0
71	1166.0
72	1001.5
73	837.0
74	690.0
75	470.5
76	398.0
77	279.5
78	161.0
79	116.0
80	71.0
81	59.0
82	47.0
83	38.0
84	29.0
85	19.5
86	10.0
87	7.5
88	5.0
89	5.5
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	465516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.38198617646087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.57184736903977	28.62215614871635
2	8.786360803302031	6.744759577874723
3	3.8478662562282997	4.430662483662745
4	2.4225941040088954	3.7193589364498014
5	1.6688271010465463	3.202644936163591
6	1.2341110822002468	2.8420580698336226
7	0.9222578962679211	2.4778662877981064
8	0.7051234106888208	2.165122960140581
9	0.5185939388337167	1.79141988523609
>10	5.2427818124493575	38.8538008262127
>50	0.05496021226459637	1.378912487752342
>100	0.019067828744859884	1.3509295618379131
>500	0.003925729447471153	0.8788791269891969
>1k	0.0016824554774876367	1.5414287113322651
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG	2788	0.5989053007845058	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGC	2368	0.5086828379690495	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	2005	0.4307048522499764	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG	691	0.14843743287019137	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	686	0.14736335593191213	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT	607	0.13039294030710008	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTT	551	0.11836327859837256	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGC	521	0.1119188169686971	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	517	0.11105955541807372	No Hit
GAATATGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	510	0.10955584770448276	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1411337096898925	0.0
2	0.0	0.0	0.0	0.7610909184646715	0.0
3	0.0	0.0	0.0	0.9756914907328642	0.0
4	0.0	0.0	0.0	1.377396265649301	0.0
5	0.0	0.0	0.0	2.947481934025898	0.0
6	0.0	0.0	0.0	3.4776463107605324	0.0
7	0.0	0.0	0.0	4.208663074953385	0.0
8	0.0	0.0	0.0	5.232258397133504	0.0
9	0.0	0.0	0.0	5.557488894044458	0.0
10	0.0	0.0	0.0	7.672776016291599	0.0
11	0.0	0.0	0.0	8.752223339262239	0.0
12	0.0	0.0	0.0	10.727880459533077	0.0
13	2.1481538765584857E-4	0.0	0.0	11.1345259883656	0.0
14	2.1481538765584857E-4	0.0	0.0	11.313896837058232	0.0
15	2.1481538765584857E-4	0.0	0.0	11.692186734720181	0.0
16	2.1481538765584857E-4	0.0	0.0	12.297966127909675	0.0
17	2.1481538765584857E-4	0.0	0.0	13.045308861564372	0.0
18	2.1481538765584857E-4	0.0	0.0	13.85215545759974	0.0
19	2.1481538765584857E-4	0.0	0.0	14.333127110561184	0.0
20	2.1481538765584857E-4	0.0	0.0	14.784883870801433	0.0
21	2.1481538765584857E-4	0.0	0.0	15.331803847773223	0.0
22	2.1481538765584857E-4	0.0	0.0	15.944242517980047	0.0
23	2.1481538765584857E-4	0.0	0.0	16.575584942300587	0.0
24	2.1481538765584857E-4	0.0	0.0	17.04366767200268	0.0
25	2.1481538765584857E-4	0.0	0.0	17.466853985684704	0.0
26	2.1481538765584857E-4	0.0	0.0	17.86125503742084	0.0
27	2.1481538765584857E-4	0.0	0.0	18.24835236597668	0.0
28	2.1481538765584857E-4	0.0	0.0	18.636738586858453	0.0
29	2.1481538765584857E-4	0.0	0.0	19.074532346901073	0.0
30	2.1481538765584857E-4	0.0	0.0	19.58385963103309	0.0
31	2.1481538765584857E-4	0.0	0.0	20.040342329801767	0.0
32	2.1481538765584857E-4	0.0	0.0	20.466321243523318	0.0
33	2.1481538765584857E-4	0.0	0.0	20.895737203447357	0.0
34	2.1481538765584857E-4	0.0	0.0	21.28777528591928	0.0
35	2.1481538765584857E-4	0.0	0.0	21.73179869220392	0.0
36	2.1481538765584857E-4	0.0	0.0	22.182696190893544	0.0
37	2.1481538765584857E-4	0.0	0.0	22.620060320160853	0.0
38	2.1481538765584857E-4	0.0	0.0	23.042172556904596	0.0
39	4.2963077531169713E-4	0.0	0.0	23.478462609233624	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	95	0.0	45.0	4
TTCAGCG	20	7.0289575E-4	45.0	1
GATCCGT	20	7.0289575E-4	45.0	35
CGCGCCG	25	3.8872175E-5	45.0	37
ACGGGCG	35	1.2098462E-7	45.0	5
CGGGCTA	40	6.7993824E-9	45.0	6
CGAATAT	40	6.7993824E-9	45.0	14
TCATCGT	25	3.8872175E-5	45.0	13
CCAACGG	20	7.0289575E-4	45.0	2
ATACCGT	20	7.0289575E-4	45.0	43
AGTACGG	40	6.7993824E-9	45.0	2
CAGTCCG	30	2.1627093E-6	44.999996	15
TCGCGCG	30	2.1627093E-6	44.999996	1
AGTAACG	30	2.1627093E-6	44.999996	1
CGCCAAT	60	0.0	44.999996	11
AATACGG	105	0.0	42.857143	5
CGTAAGG	80	0.0	42.1875	2
AACGGCT	75	0.0	42.000004	7
ACGGCTG	605	0.0	40.537193	8
CGACGGG	95	0.0	40.263157	3
>>END_MODULE