##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936116.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390090
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.883473044681995	31.0	31.0	34.0	30.0	34.0
2	32.01035402086698	33.0	31.0	34.0	30.0	34.0
3	31.94404368222718	33.0	31.0	34.0	30.0	34.0
4	35.67586967110154	37.0	35.0	37.0	33.0	37.0
5	31.27865620754185	37.0	35.0	37.0	0.0	37.0
6	33.468607244482044	37.0	35.0	37.0	17.0	37.0
7	29.46185495654849	35.0	32.0	37.0	0.0	37.0
8	32.61377630803148	35.0	33.0	37.0	17.0	37.0
9	36.50497321131021	39.0	35.0	39.0	32.0	39.0
10	36.8311517854854	38.0	37.0	39.0	33.0	39.0
11	36.935281601681666	39.0	37.0	39.0	33.0	39.0
12	36.97846138070702	39.0	37.0	39.0	33.0	39.0
13	36.821643723243355	39.0	37.0	39.0	33.0	39.0
14	38.063375118562384	40.0	37.0	41.0	33.0	41.0
15	38.18077623112615	40.0	37.0	41.0	33.0	41.0
16	38.22813709656746	40.0	37.0	41.0	34.0	41.0
17	38.1917531851624	40.0	37.0	41.0	34.0	41.0
18	38.126991207157324	40.0	37.0	41.0	33.0	41.0
19	38.05529493193878	40.0	37.0	41.0	34.0	41.0
20	37.98853854238765	40.0	37.0	41.0	33.0	41.0
21	37.911079494475636	40.0	37.0	41.0	33.0	41.0
22	37.87949447563383	40.0	36.0	41.0	33.0	41.0
23	37.850239688277064	40.0	36.0	41.0	33.0	41.0
24	37.81952369965905	40.0	36.0	41.0	33.0	41.0
25	37.679427824348224	40.0	36.0	41.0	33.0	41.0
26	37.51480427593632	39.0	36.0	41.0	33.0	41.0
27	37.48942808069933	39.0	36.0	41.0	33.0	41.0
28	37.34851957240637	39.0	36.0	41.0	32.0	41.0
29	37.34322848573406	39.0	36.0	41.0	32.0	41.0
30	37.261326873285654	39.0	36.0	41.0	32.0	41.0
31	37.11089235817376	39.0	35.0	41.0	31.0	41.0
32	37.01648337563127	39.0	35.0	41.0	31.0	41.0
33	36.85850444769156	39.0	35.0	41.0	31.0	41.0
34	36.70622933169269	39.0	35.0	41.0	30.0	41.0
35	36.623558665948885	39.0	35.0	41.0	30.0	41.0
36	36.50350688302699	39.0	35.0	41.0	30.0	41.0
37	36.30577815375939	39.0	35.0	40.0	30.0	41.0
38	36.234207490579095	39.0	35.0	40.0	30.0	41.0
39	36.2559101745751	39.0	35.0	40.0	30.0	41.0
40	36.10035632802686	39.0	35.0	40.0	29.0	41.0
41	36.01081545284421	39.0	35.0	40.0	29.0	41.0
42	35.9413186700505	39.0	35.0	40.0	29.0	41.0
43	35.728242200517826	38.0	35.0	40.0	27.0	41.0
44	35.456874054705324	38.0	35.0	40.0	26.0	41.0
45	35.42154374631495	38.0	34.0	40.0	26.0	41.0
46	35.29121484785562	38.0	34.0	40.0	26.0	41.0
47	35.168420108180165	38.0	34.0	40.0	25.0	41.0
48	35.05232638621857	38.0	34.0	40.0	24.0	41.0
49	34.879491912122845	38.0	34.0	40.0	24.0	41.0
50	34.683608910764185	38.0	34.0	40.0	23.0	41.0
51	32.60094337204235	36.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	13.0
10	15.0
11	13.0
12	12.0
13	12.0
14	7.0
15	18.0
16	21.0
17	65.0
18	134.0
19	282.0
20	453.0
21	797.0
22	1205.0
23	1801.0
24	2475.0
25	3224.0
26	4000.0
27	4902.0
28	5794.0
29	6807.0
30	8218.0
31	10308.0
32	13445.0
33	18724.0
34	26589.0
35	32964.0
36	38303.0
37	60463.0
38	75925.0
39	73086.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37429823886795	20.697531338921788	25.81301750878002	12.115152913430235
2	30.415545130610884	25.61152554538696	27.489810043836037	16.483119280166115
3	32.27203978569048	24.56971468122741	27.23756056294701	15.920684970135095
4	28.451639365274676	26.32853956779205	26.734087005562817	18.485734061370454
5	21.363787843830913	39.5593324617396	21.94006511317901	17.13681458125048
6	25.486938911533237	34.8524699428337	24.66071932118229	14.999871824450766
7	70.08023789381937	19.65084980389141	6.4023686841498115	3.8665436181394037
8	85.07985336717168	4.465123433053911	6.91532723217719	3.5396959675972215
9	78.65031146658464	8.05301340716245	8.550847240380426	4.745827885872491
10	37.864595349791074	35.93298982286139	14.757620036401855	11.44479479094568
11	27.34394626880976	26.228562639390912	29.506267784357455	16.92122330744187
12	26.791509651618856	24.500499884642004	31.051808556999667	17.65618190673947
13	23.140557307288063	26.551565023456124	31.488630828783105	18.81924684047271
14	18.844112897023763	30.416570535004745	29.942321002845496	20.796995565125997
15	17.690532953933708	28.83308979978979	35.26032454049066	18.216052705785845
16	21.600399907713605	26.93250275577431	32.36304442564536	19.104052910866724
17	21.16588479581635	26.650516547463404	28.963828860006664	23.219769796713578
18	22.1384808633905	27.567740777769234	30.18739265297752	20.10638570586275
19	23.155169319900537	28.19118664923479	28.729780307108616	19.923863723756057
20	24.26132430977467	27.88766694865287	29.22992130021277	18.621087441359684
21	24.196980184060088	27.311646030403242	29.69186597964572	18.799507805890947
22	21.944423081852907	26.14627393678382	29.16070650362737	22.74859647773591
23	21.230485272629394	27.638750032043884	29.098413186700505	22.032351508626213
24	21.44197492886257	26.278807454689947	30.85185470019739	21.427362916250097
25	19.40398369607014	29.439103796559767	29.421671921864185	21.735240585505906
26	20.204055474377707	30.599861570406826	28.379604706606166	20.816478248609297
27	21.521700120485015	29.286062190776484	29.545489502422516	19.64674818631598
28	18.176830987720784	28.680817247301903	31.9877464174934	21.154605347483916
29	20.32146427747443	27.488528288343716	30.698813094414106	21.49119433976775
30	21.776000410161757	27.600810069471148	29.974108539055088	20.649080981312004
31	22.521213053397933	28.336537722064136	28.667743341280215	20.47450588325771
32	23.632495065241354	28.414981158194262	28.473941910841088	19.478581865723292
33	23.91243046476454	27.57850752390474	28.799251454792486	19.709810556538233
34	21.04181086415955	27.39572919069958	30.375041657053504	21.187418288087365
35	22.42277423158758	26.573354866825603	29.671870593965494	21.332000307621318
36	24.256709990002307	27.965084980389143	28.566228306288295	19.21197672332026
37	22.32202824989105	27.668486759465765	31.43120818272706	18.578276807916122
38	22.026455433361534	27.531595272885745	29.228639544720448	21.213309749032273
39	22.750647286523623	26.651541951857265	29.932323310005383	20.66548745161373
40	23.341536568484194	26.199338614165963	30.74957061191007	19.70955420543977
41	21.92904201594504	26.6433387167064	29.096362377912786	22.33125688943577
42	22.696557204747624	26.60847496731524	28.955881975954266	21.739085851982875
43	23.665308005844803	25.9242738855136	29.42423543284883	20.986182675792765
44	21.470429900792126	27.555948627239868	29.06303673511241	21.910584736855597
45	22.1269450639596	26.659232484811195	28.760286087825886	22.453536363403316
46	22.552744238509064	27.488784639442184	28.596734087005565	21.361737035043195
47	22.072085928888203	26.808428824117513	30.324796841754463	20.794688405239818
48	22.412007485452076	25.370042810633443	29.77338562895742	22.44456407495706
49	21.93827065548976	25.159322207695663	31.673972672972905	21.228434463841676
50	21.86700505011664	25.516162936758185	30.42041580148171	22.196416211643466
51	21.43274628931785	25.147530057166296	28.852828834371557	24.5668948191443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	518.0
1	731.0
2	944.0
3	1225.5
4	1507.0
5	1247.0
6	987.0
7	862.0
8	737.0
9	763.5
10	790.0
11	855.0
12	920.0
13	932.0
14	944.0
15	952.0
16	960.0
17	1051.0
18	1142.0
19	1190.0
20	1238.0
21	1588.0
22	1938.0
23	2243.5
24	2549.0
25	3068.0
26	4303.5
27	5020.0
28	5760.0
29	6500.0
30	7518.5
31	8537.0
32	10148.0
33	11759.0
34	12996.5
35	14234.0
36	15276.5
37	16319.0
38	17720.0
39	19121.0
40	21429.5
41	23738.0
42	25844.5
43	27951.0
44	29932.0
45	31913.0
46	33507.0
47	35101.0
48	36111.5
49	37122.0
50	35058.0
51	32994.0
52	28787.0
53	24580.0
54	21428.0
55	18276.0
56	16221.5
57	14167.0
58	13195.5
59	12224.0
60	11716.0
61	11208.0
62	9507.0
63	7806.0
64	6461.5
65	5117.0
66	4089.0
67	3061.0
68	2474.0
69	1887.0
70	1495.5
71	1104.0
72	862.0
73	620.0
74	523.5
75	345.0
76	263.0
77	193.5
78	124.0
79	96.0
80	68.0
81	56.0
82	44.0
83	30.5
84	17.0
85	14.5
86	12.0
87	9.5
88	7.0
89	4.5
90	2.0
91	2.0
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	390090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.54440954741399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65988744936193	30.60156531412712
2	9.303330753871995	7.730027659878198
3	4.221199639207194	5.261017397778597
4	2.533680020792473	4.210409617836115
5	1.7428668718128988	3.620318755460768
6	1.2632520808000611	3.148863708382839
7	0.8953163630360018	2.603677276232819
8	0.7332652932124665	2.437045891849865
9	0.6149663926169552	2.299357410549711
>10	4.977750877510254	35.836154049683
>50	0.03917969098626453	1.1101403070184663
>100	0.0146923841198492	1.0140044453797536
>500	6.121826716603833E-4	0.12741816582273804
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	501	0.1284319003306929	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	489	0.12535568714911943	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	428	0.10971827014278755	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.127021969289138E-4	0.0	0.0	0.14381296623856032	0.0
2	5.127021969289138E-4	0.0	0.0	0.7159886180112282	0.0
3	5.127021969289138E-4	0.0	0.0	0.9541387884847087	0.0
4	5.127021969289138E-4	0.0	0.0	1.2350995924017534	0.0
5	5.127021969289138E-4	0.0	0.0	2.0872106436976083	0.0
6	5.127021969289138E-4	0.0	0.0	2.6076033735804556	0.0
7	5.127021969289138E-4	0.0	0.0	3.121074623804763	0.0
8	5.127021969289138E-4	0.0	0.0	3.9498577251403524	0.0
9	5.127021969289138E-4	0.0	0.0	4.27926888666718	0.0
10	5.127021969289138E-4	0.0	0.0	5.119331436335204	0.0
11	5.127021969289138E-4	0.0	0.0	6.243431003101849	0.0
12	5.127021969289138E-4	0.0	0.0	7.1509138916660255	0.0
13	5.127021969289138E-4	0.0	0.0	7.514419749288626	0.0
14	5.127021969289138E-4	0.0	0.0	7.675920941321234	0.0
15	5.127021969289138E-4	0.0	0.0	7.954574585352098	0.0
16	5.127021969289138E-4	0.0	0.0	8.55289804916814	0.0
17	5.127021969289138E-4	0.0	0.0	9.33733241046938	0.0
18	5.127021969289138E-4	0.0	0.0	10.118690558589044	0.0
19	5.127021969289138E-4	0.0	0.0	10.596785357225256	0.0
20	5.127021969289138E-4	0.0	0.0	11.06693327180907	0.0
21	5.127021969289138E-4	0.0	0.0	11.61962624009844	0.0
22	5.127021969289138E-4	0.0	0.0	12.25588966648722	0.0
23	5.127021969289138E-4	0.0	0.0	12.854213130303263	0.0
24	5.127021969289138E-4	0.0	0.0	13.336665897613372	0.0
25	5.127021969289138E-4	0.0	0.0	13.772975467199878	0.0
26	5.127021969289138E-4	0.0	0.0	14.162629136865851	0.0
27	5.127021969289138E-4	0.0	0.0	14.57330359660591	0.0
28	5.127021969289138E-4	0.0	0.0	14.978594683278217	0.0
29	5.127021969289138E-4	0.0	0.0	15.405931964418468	0.0
30	5.127021969289138E-4	0.0	0.0	15.891717296008613	0.0
31	7.690532953933708E-4	0.0	0.0	16.366735881463253	0.0
32	7.690532953933708E-4	0.0	0.0	16.79176600271732	0.0
33	7.690532953933708E-4	0.0	0.0	17.218846932759106	0.0
34	7.690532953933708E-4	0.0	0.0	17.63439206336999	0.0
35	7.690532953933708E-4	0.0	0.0	18.08300648568279	0.0
36	7.690532953933708E-4	0.0	0.0	18.544182111820348	0.0
37	7.690532953933708E-4	0.0	0.0	18.97331385064985	0.0
38	7.690532953933708E-4	0.0	0.0	19.39962572739624	0.0
39	7.690532953933708E-4	0.0	0.0	19.826706657438027	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTG	25	3.886051E-5	45.0	1
AAGTACG	20	7.02755E-4	45.0	1
GTCGATG	25	3.886051E-5	45.0	1
CTATCCC	20	7.02755E-4	45.0	38
TAGCGGA	20	7.02755E-4	45.0	3
ATTAACG	20	7.02755E-4	45.0	1
CGTCTCA	20	7.02755E-4	45.0	34
ACGGGTG	55	1.8189894E-12	45.0	5
CAGGGTA	25	3.886051E-5	45.0	5
AACATCG	20	7.02755E-4	45.0	13
CGAATAT	35	1.2091732E-7	45.0	14
ATGCGAA	25	3.886051E-5	45.0	26
ATAACGG	35	1.2091732E-7	45.0	2
ACGTAGG	25	3.886051E-5	45.0	2
AGCTACG	35	1.2091732E-7	45.0	9
TATACGG	35	1.2091732E-7	45.0	2
TTACCGG	20	7.02755E-4	45.0	2
AAGGCGC	25	3.886051E-5	45.0	30
TCGAATC	20	7.02755E-4	45.0	17
TAATCGC	20	7.02755E-4	45.0	43
>>END_MODULE