##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936113.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	496586
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896946349675584	31.0	31.0	34.0	30.0	34.0
2	32.0273165171793	33.0	31.0	34.0	30.0	34.0
3	31.985245254598397	33.0	31.0	34.0	30.0	34.0
4	35.70795390929265	37.0	35.0	37.0	35.0	37.0
5	31.192131876452418	37.0	35.0	37.0	0.0	37.0
6	33.402798306839095	37.0	35.0	37.0	17.0	37.0
7	29.541392628869925	35.0	32.0	37.0	0.0	37.0
8	32.57741055929889	35.0	33.0	37.0	17.0	37.0
9	36.394602344810366	39.0	35.0	39.0	32.0	39.0
10	36.775299746670264	38.0	35.0	39.0	33.0	39.0
11	36.93354424007121	39.0	37.0	39.0	33.0	39.0
12	36.93792213231948	39.0	37.0	39.0	33.0	39.0
13	36.77758535278884	39.0	37.0	39.0	33.0	39.0
14	38.03898015650864	40.0	37.0	41.0	33.0	41.0
15	38.13602276342869	40.0	37.0	41.0	33.0	41.0
16	38.17431421747693	40.0	37.0	41.0	33.0	41.0
17	38.14938802141019	40.0	37.0	41.0	33.0	41.0
18	38.06242020516084	40.0	37.0	41.0	33.0	41.0
19	37.97818504750436	40.0	37.0	41.0	33.0	41.0
20	37.922363900714075	40.0	37.0	41.0	33.0	41.0
21	37.82574619501959	40.0	36.0	41.0	33.0	41.0
22	37.80650682862586	40.0	36.0	41.0	33.0	41.0
23	37.749461321905976	40.0	36.0	41.0	33.0	41.0
24	37.701894535891064	40.0	36.0	41.0	33.0	41.0
25	37.568215777327595	39.0	36.0	41.0	33.0	41.0
26	37.39989649325595	39.0	36.0	41.0	32.0	41.0
27	37.35596251203216	39.0	36.0	41.0	32.0	41.0
28	37.22582593951501	39.0	36.0	41.0	32.0	41.0
29	37.21420660268312	39.0	36.0	41.0	32.0	41.0
30	37.096670063191475	39.0	35.0	41.0	31.0	41.0
31	36.983448989701685	39.0	35.0	41.0	31.0	41.0
32	36.896807400933575	39.0	35.0	41.0	31.0	41.0
33	36.69219027519906	39.0	35.0	41.0	30.0	41.0
34	36.58199586778524	39.0	35.0	41.0	30.0	41.0
35	36.484850559621094	39.0	35.0	41.0	30.0	41.0
36	36.32352301514743	39.0	35.0	40.0	30.0	41.0
37	36.2557321390454	39.0	35.0	40.0	30.0	41.0
38	36.144651681682525	39.0	35.0	40.0	29.0	41.0
39	36.11266326477186	39.0	35.0	40.0	29.0	41.0
40	35.93968819096793	39.0	35.0	40.0	28.0	41.0
41	35.79371146186159	38.0	35.0	40.0	28.0	41.0
42	35.71239221403745	38.0	35.0	40.0	27.0	41.0
43	35.55814300040678	38.0	34.0	40.0	27.0	41.0
44	35.420817340803005	38.0	34.0	40.0	26.0	41.0
45	35.320315111581884	38.0	34.0	40.0	26.0	41.0
46	35.15063251883863	38.0	34.0	40.0	26.0	41.0
47	35.00263398484854	38.0	34.0	40.0	25.0	41.0
48	34.896535544699205	38.0	34.0	40.0	24.0	41.0
49	34.714766425150934	38.0	34.0	40.0	24.0	41.0
50	34.53946748398062	37.0	33.0	40.0	24.0	41.0
51	32.56192079518956	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	32.0
10	33.0
11	22.0
12	19.0
13	19.0
14	34.0
15	36.0
16	58.0
17	123.0
18	233.0
19	422.0
20	764.0
21	1173.0
22	1649.0
23	2340.0
24	3186.0
25	4148.0
26	5135.0
27	6134.0
28	7168.0
29	8694.0
30	10661.0
31	13775.0
32	17607.0
33	25103.0
34	35299.0
35	42431.0
36	50545.0
37	77516.0
38	94362.0
39	87843.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.17948552717958	20.130450717499084	23.777150382813854	10.91291337250748
2	32.627983873890926	24.765096076006976	24.960631189763706	17.64628886033839
3	32.95582235504021	24.757041076470138	25.83721651436005	16.449920054129596
4	29.948891027938767	25.760291268783252	25.113273430986776	19.177544272291204
5	22.123257602912688	39.776795962834235	20.6018695653925	17.49807686886058
6	26.325953611257667	36.886460754028505	23.283781661182555	13.503803973531273
7	69.17110027266173	20.011035349365468	6.615168369627819	4.202696008344979
8	82.36096063924477	6.252693390470131	7.4327508226168275	3.953595147668279
9	75.67108214891277	8.887080988992842	9.65935406958714	5.782482792507239
10	40.606863665105344	28.91926071214251	16.82004728284728	13.65382833990487
11	32.01278328426496	26.9687425742973	23.11321704599002	17.905257095447716
12	31.229434579307515	23.608800892493946	26.757097461466895	18.404667066731644
13	25.270144546966687	25.8559443882832	27.895268896022042	20.978642168728076
14	19.83765148433504	29.023170206167713	28.08496413511456	23.054214174382683
15	19.79073111203296	28.05878538661984	32.4247965105742	19.725686990773
16	22.577358201801903	25.83016838976532	31.789458422106144	19.803014986326637
17	22.46116483348302	25.937098508616835	28.368298743822823	23.23343791407732
18	23.230417289251008	27.376124175872864	28.011059514364078	21.382399020512054
19	25.08649055752679	28.749904346880502	25.42077303830555	20.742832057287156
20	27.93675214363675	25.78606726730113	27.280672431361335	18.99650815770078
21	26.16686737040513	27.117357315751956	26.18921999411985	20.52655531972307
22	24.624133584112318	25.592344528440194	26.115516748357788	23.668005139089704
23	23.67283813881181	27.593206413390632	27.53883516651698	21.195120281280584
24	23.521404147519263	25.20510042570673	29.351814187270687	21.92168123950333
25	23.25498503783836	26.59217134595015	27.185824811815074	22.967018804396417
26	22.783163439968103	28.24546000088605	26.43771672983129	22.53365982931456
27	23.051596299533212	29.030016955774023	26.95947932482994	20.958907419862825
28	21.24345027850161	28.14275875679137	28.964972834514064	21.648818130192957
29	23.129931170028957	26.962701324644673	27.893859271103093	22.013508234223274
30	23.300495785221493	26.98424844840571	26.737765462578484	22.97749030379431
31	23.809974505926466	27.86707639764311	25.982206506023125	22.3407425904073
32	25.794927766791652	27.86325027286311	25.32995291852771	21.01186904181753
33	24.368387348817727	28.135307882219795	26.046445127329402	21.449859641633072
34	23.747346884527552	27.327592803663414	27.20414993576138	21.72091037604765
35	24.685955705557546	25.87104751241477	26.677755716029043	22.76524106599864
36	23.384469155393024	28.84656434132255	26.38032485813132	21.388641645153108
37	24.035111742981073	28.24384900097868	27.510441293149622	20.210597962890617
38	23.54234714631504	27.530578791991722	26.778644585227934	22.148429476465306
39	24.51700209027238	26.18801174418933	26.871478454889992	22.42350771064831
40	25.375866415887682	25.713975021446434	28.40253249185438	20.5076260708115
41	22.08056610536745	26.428453480363924	28.396692617190173	23.094287797078454
42	22.830687937235446	27.113329815983533	27.433918797549666	22.62206344923135
43	23.5640956450645	26.600427720475405	27.499365668786474	22.33611096567362
44	22.754165441635486	27.231134184209782	26.962701324644673	23.051999049510055
45	23.399975029501434	26.77361021051741	25.96388138207682	23.86253337790433
46	22.865123060255424	28.533828984304833	25.910517010145274	22.69053094529447
47	23.446291276838252	26.366429983930278	28.897512213393046	21.289766525838424
48	22.715501443858667	25.575831779389674	28.565646232475338	23.143020544276318
49	21.858449493139155	26.56981872223542	28.834481842017297	22.73724994260813
50	20.46795519809258	26.76474971102689	28.718489848686836	24.048805242193698
51	21.08678053751012	25.609058652479128	27.627641536410614	25.67651927360014
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	405.0
1	712.0
2	1019.0
3	1479.5
4	1940.0
5	1548.0
6	1156.0
7	1051.5
8	947.0
9	951.0
10	955.0
11	1015.5
12	1076.0
13	1092.0
14	1108.0
15	1153.0
16	1198.0
17	1202.0
18	1206.0
19	1288.0
20	1370.0
21	1734.0
22	2098.0
23	2343.5
24	2589.0
25	3219.5
26	4723.5
27	5597.0
28	6801.5
29	8006.0
30	9040.5
31	10075.0
32	11552.0
33	13029.0
34	14477.0
35	15925.0
36	16913.0
37	17901.0
38	19184.0
39	20467.0
40	22419.5
41	24372.0
42	26183.5
43	27995.0
44	29539.0
45	31083.0
46	32395.0
47	33707.0
48	34946.0
49	36185.0
50	36876.5
51	37568.0
52	36690.5
53	35813.0
54	32620.0
55	29427.0
56	27549.5
57	25672.0
58	25168.5
59	24665.0
60	24137.0
61	23609.0
62	21767.5
63	19926.0
64	16663.0
65	13400.0
66	10684.0
67	7968.0
68	6411.5
69	4855.0
70	4076.5
71	3298.0
72	2601.5
73	1905.0
74	1655.0
75	1090.5
76	776.0
77	594.5
78	413.0
79	328.5
80	244.0
81	204.5
82	165.0
83	135.0
84	105.0
85	87.5
86	70.0
87	47.0
88	24.0
89	14.5
90	5.0
91	6.0
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	496586.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.38623187379629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3111588308434	27.757529099436674
2	8.86480465407908	6.805728939347762
3	4.3420492456652475	5.000247274546455
4	2.811204102499338	4.31646130092428
5	1.9386190076966952	3.720813937219711
6	1.3924054907586711	3.206952001836569
7	1.0326741211337531	2.774832778473633
8	0.8327255729379731	2.557215754402952
9	0.6463209309697306	2.232884260898277
>10	5.758773281939438	38.96572270745429
>50	0.049995617306509366	1.277429798068839
>100	0.018748356489932796	1.250241933821559
>500	5.207876802759109E-4	0.13394021356898697
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	670	0.13492124224202856	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06967574599364461	0.0
2	0.0	0.0	0.0	0.33690035562822956	0.0
3	0.0	0.0	0.0	0.4663844731828928	0.0
4	0.0	0.0	0.0	0.6260748390006967	0.0
5	0.0	0.0	0.0	1.1512608088025036	0.0
6	0.0	0.0	0.0	1.5151454128791388	0.0
7	0.0	0.0	0.0	1.8375467693410608	0.0
8	0.0	0.0	0.0	2.4320057351596702	0.0
9	0.0	0.0	0.0	2.67023234646164	0.0
10	0.0	0.0	0.0	3.270329811956036	0.0
11	0.0	0.0	0.0	3.9904467705493105	0.0
12	0.0	0.0	0.0	4.585711236321604	0.0
13	0.0	0.0	0.0	4.837429971847777	0.0
14	0.0	0.0	0.0	4.944360090699295	0.0
15	0.0	0.0	0.0	5.164865703020222	0.0
16	0.0	0.0	0.0	5.62581305151575	0.0
17	0.0	0.0	0.0	6.112536398529157	0.0
18	0.0	0.0	0.0	6.650408992601483	0.0
19	0.0	0.0	0.0	6.979052973704454	0.0
20	0.0	2.0137498842093816E-4	0.0	7.305884579911637	0.0
21	2.0137498842093816E-4	2.0137498842093816E-4	0.0	7.745284804646124	0.0
22	2.0137498842093816E-4	2.0137498842093816E-4	0.0	8.197573028639551	0.0
23	2.0137498842093816E-4	2.0137498842093816E-4	0.0	8.627105878941412	0.0
24	2.0137498842093816E-4	2.0137498842093816E-4	0.0	8.967026859395956	0.0
25	2.0137498842093816E-4	2.0137498842093816E-4	0.0	9.283789716182092	0.0
26	2.0137498842093816E-4	2.0137498842093816E-4	0.0	9.57115182465877	0.0
27	2.0137498842093816E-4	2.0137498842093816E-4	0.0	9.861333182973341	0.0
28	2.0137498842093816E-4	2.0137498842093816E-4	0.0	10.145070541658443	0.0
29	2.0137498842093816E-4	2.0137498842093816E-4	0.0	10.445320649394063	0.0
30	2.0137498842093816E-4	2.0137498842093816E-4	0.0	10.811219003354907	0.0
31	2.0137498842093816E-4	2.0137498842093816E-4	0.0	11.164833483022075	0.0
32	2.0137498842093816E-4	2.0137498842093816E-4	0.0	11.493880214101887	0.0
33	2.0137498842093816E-4	2.0137498842093816E-4	0.0	11.821517320262753	0.0
34	4.027499768418763E-4	2.0137498842093816E-4	0.0	12.14492555166678	0.0
35	4.027499768418763E-4	2.0137498842093816E-4	0.0	12.489075406878165	0.0
36	4.027499768418763E-4	2.0137498842093816E-4	0.0	12.84893251118638	0.0
37	4.027499768418763E-4	2.0137498842093816E-4	0.0	13.198318116096708	0.0
38	4.027499768418763E-4	2.0137498842093816E-4	0.0	13.539245971493356	0.0
39	4.027499768418763E-4	2.0137498842093816E-4	0.0	13.889034326380527	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGG	35	1.2100645E-7	45.000004	2
TAGGCGA	35	1.2100645E-7	45.000004	6
TCGTTAG	20	7.029412E-4	45.0	1
CGATTAC	20	7.029412E-4	45.0	10
TCGATTG	20	7.029412E-4	45.0	12
TCGCTTA	20	7.029412E-4	45.0	28
ATCGCAT	20	7.029412E-4	45.0	38
TACGGGA	150	0.0	40.5	4
TATGCGG	45	1.9244908E-8	40.0	2
GCACCGA	45	1.9244908E-8	40.0	9
TACGAAT	85	0.0	39.705883	12
CGAATAT	85	0.0	39.705883	14
CGGTCTA	40	3.452933E-7	39.375	31
CGGTAGT	80	0.0	39.375	12
CACGCCG	75	0.0	39.0	26
CACGACC	70	0.0	38.57143	27
TGTAACG	35	6.241122E-6	38.57143	1
TACCGGG	35	6.241122E-6	38.57143	3
AGTACGG	65	9.094947E-12	38.07692	2
TGATATG	60	1.546141E-10	37.499996	1
>>END_MODULE