##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936112.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476419
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90763382652665	31.0	31.0	34.0	30.0	34.0
2	32.04595954401483	33.0	31.0	34.0	30.0	34.0
3	31.987464815634976	33.0	31.0	34.0	30.0	34.0
4	35.71009342616478	37.0	35.0	37.0	35.0	37.0
5	31.24456833165764	37.0	35.0	37.0	0.0	37.0
6	33.43143955215892	37.0	35.0	37.0	17.0	37.0
7	29.46852245607333	35.0	32.0	37.0	0.0	37.0
8	32.53782699682422	35.0	33.0	37.0	17.0	37.0
9	36.3860509341567	39.0	35.0	39.0	32.0	39.0
10	36.7665941114859	38.0	35.0	39.0	33.0	39.0
11	36.93077312197876	39.0	37.0	39.0	33.0	39.0
12	36.92057621547419	39.0	35.0	39.0	33.0	39.0
13	36.74077020437892	39.0	35.0	39.0	33.0	39.0
14	37.96498670288129	40.0	37.0	41.0	33.0	41.0
15	38.06086449113071	40.0	37.0	41.0	33.0	41.0
16	38.09555664236733	40.0	37.0	41.0	33.0	41.0
17	38.046142156379155	40.0	37.0	41.0	33.0	41.0
18	37.968613762255494	40.0	37.0	41.0	33.0	41.0
19	37.89977519788253	40.0	36.0	41.0	33.0	41.0
20	37.83422155707476	40.0	36.0	41.0	33.0	41.0
21	37.747816522850684	40.0	36.0	41.0	33.0	41.0
22	37.727026000222494	39.0	36.0	41.0	33.0	41.0
23	37.67568883692716	39.0	36.0	41.0	33.0	41.0
24	37.62062386260833	39.0	36.0	41.0	33.0	41.0
25	37.4826675678342	39.0	35.0	41.0	33.0	41.0
26	37.31550378973131	39.0	35.0	41.0	32.0	41.0
27	37.24410865225778	39.0	35.0	41.0	32.0	41.0
28	37.101876709367176	39.0	35.0	41.0	32.0	41.0
29	37.07021130559445	39.0	35.0	41.0	32.0	41.0
30	36.93898228240268	39.0	35.0	41.0	31.0	41.0
31	36.79344022803457	39.0	35.0	41.0	31.0	41.0
32	36.69601128418472	39.0	35.0	40.0	31.0	41.0
33	36.545696120431806	39.0	35.0	40.0	30.0	41.0
34	36.36750843270315	39.0	35.0	40.0	30.0	41.0
35	36.29157107504109	39.0	35.0	40.0	30.0	41.0
36	36.16535654539386	39.0	35.0	40.0	30.0	41.0
37	36.06566698641322	38.0	35.0	40.0	29.0	41.0
38	35.98679313797309	38.0	35.0	40.0	29.0	41.0
39	35.916298468365035	38.0	35.0	40.0	29.0	41.0
40	35.700992193846176	38.0	35.0	40.0	27.0	41.0
41	35.58019096635525	38.0	35.0	40.0	27.0	41.0
42	35.5527634288305	38.0	35.0	40.0	27.0	41.0
43	35.362111922488396	38.0	34.0	40.0	26.0	41.0
44	35.11001450403951	38.0	34.0	40.0	25.0	41.0
45	34.961305069697055	38.0	34.0	40.0	24.0	41.0
46	34.79001047397354	38.0	34.0	40.0	24.0	41.0
47	34.62821801817308	38.0	33.0	40.0	23.0	41.0
48	34.54530150980545	37.0	33.0	40.0	23.0	41.0
49	34.393909562800815	37.0	33.0	40.0	23.0	41.0
50	34.201847533368735	37.0	33.0	40.0	23.0	41.0
51	32.15687031793442	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	26.0
10	25.0
11	20.0
12	18.0
13	10.0
14	21.0
15	42.0
16	55.0
17	136.0
18	244.0
19	426.0
20	739.0
21	1142.0
22	1707.0
23	2420.0
24	3238.0
25	4160.0
26	5189.0
27	6325.0
28	7271.0
29	8798.0
30	10862.0
31	13830.0
32	17960.0
33	25134.0
34	35807.0
35	42976.0
36	48258.0
37	73213.0
38	86968.0
39	79373.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03014993104809	20.70950151022524	23.666982215234906	11.593366343491757
2	32.19372023365987	24.72739332394384	25.056515378269967	18.022371064126325
3	32.90905694357278	23.831963040936653	26.306885325732182	16.952094689758386
4	29.275070893478222	26.219777128955812	25.841118847065292	18.664033130500673
5	22.461950509950274	39.180637212201866	21.118175387631474	17.239236890216386
6	26.5476397876659	36.03298776916958	23.649560575879637	13.76981186728489
7	69.02117673728377	19.89005476271937	6.941158937825738	4.147609562171114
8	82.48768416037143	5.452553319661894	7.918449935875774	4.141312584090895
9	76.1657280671006	8.132966989141911	9.980080559339573	5.721224384417918
10	41.44167214153928	26.252101616434274	17.159685067136284	15.14654117489017
11	31.24917352162697	26.249163026663503	24.016674397956418	18.484989053753104
12	29.545001353850285	24.015205103071036	27.90274947052909	18.537044072549584
13	24.853542784817567	25.291182761392804	29.872864012560374	19.982410441229252
14	20.63351797472393	27.899810880758324	29.667582527145225	21.799088617372522
15	20.48364989641471	25.475684219143236	33.55071900994713	20.48994687449493
16	23.43000594014932	24.80022837040504	31.074327430266212	20.69543825917942
17	22.58663067593862	24.686672865691754	28.6428542942242	24.083842164145427
18	24.396592075462987	25.058614370963372	29.470906911773042	21.0738866418006
19	24.822057894416467	27.15193978409761	27.006479590444542	21.019522731041373
20	26.29576066445713	25.4719060322951	27.50834874343802	20.723984559809747
21	25.100804124100843	26.597805713038312	28.38509799147389	19.91629217138695
22	23.16469326370275	25.288873869430063	27.886377327520524	23.660055539346665
23	23.345206635335703	26.39693211227932	27.795281044626684	22.462580207758297
24	23.5460802360947	24.77294146539076	29.320199236386458	22.360779062128085
25	22.602373121139166	26.07536643164945	27.358900463667485	23.963359983543896
26	21.618365346470227	28.180656103136105	27.005639993367183	23.19533855702648
27	21.199826203404985	28.151270205428414	28.13342876753446	22.51547482363214
28	20.345955975727247	28.144973227348196	29.173899445656033	22.335171351268528
29	22.55808437530829	26.63537768225029	28.239637797820826	22.566900144620597
30	23.32043852155351	26.995145029900154	28.01609507597304	21.668321372573303
31	24.42786706659474	27.577195703781754	26.15869644157769	21.836240788045817
32	25.518923468627406	27.847755861961843	25.15432843778271	21.47899223162804
33	24.791832399631417	26.85367292236456	26.014285744271326	22.340208933732704
34	22.62147395464916	27.169571322722224	28.07486687138842	22.13408785124019
35	23.470726398401407	26.918741695860156	26.71681859875446	22.89371330698398
36	23.023221156167157	29.16718266903713	26.102443437394395	21.707152737401323
37	22.288783612744247	29.316630948807664	26.912024919241258	21.482560519206835
38	22.726843387858167	28.442610391273227	25.189591515031935	23.64095470583667
39	24.493355638629023	25.91815187891331	26.64230435813853	22.94618812431914
40	23.200376139490658	25.75631954225167	28.27595037141676	22.76735394684091
41	20.133118116615837	26.68197532004391	27.713630228853177	25.47127633448708
42	21.458422103232657	26.941200917679602	26.681555521505228	24.918821457582506
43	22.700815878459927	26.105591926434503	26.61606694947095	24.57752524563462
44	21.93804193367603	26.93007625640455	26.956943362880153	24.174938447039267
45	23.203104829992085	25.74225629120585	26.51258660968601	24.542052269116052
46	22.94954651262859	26.586471152493917	26.695198974012374	23.768783360865122
47	21.584571564106387	26.269313356520207	28.388876178322025	23.757238901051387
48	21.4069967822442	25.303566818283908	29.200556652862293	24.0888797466096
49	21.777468992630435	25.67005094255267	28.39160486882345	24.16087519599344
50	20.73363992619942	25.612748442022674	28.992126678407033	24.661484953370877
51	20.40178918137186	24.9694071814936	28.14245443611611	26.486349201018434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	413.0
1	727.0
2	1041.0
3	1465.5
4	1890.0
5	1478.5
6	1067.0
7	976.0
8	885.0
9	946.5
10	1008.0
11	948.5
12	889.0
13	979.5
14	1070.0
15	1115.5
16	1161.0
17	1173.5
18	1186.0
19	1269.5
20	1353.0
21	1530.0
22	1707.0
23	1962.0
24	2217.0
25	2877.0
26	4337.5
27	5138.0
28	5997.0
29	6856.0
30	7860.0
31	8864.0
32	9768.5
33	10673.0
34	12347.5
35	14022.0
36	14762.0
37	15502.0
38	16634.0
39	17766.0
40	20211.5
41	22657.0
42	25374.0
43	28091.0
44	30655.0
45	33219.0
46	35873.5
47	38528.0
48	39332.0
49	40136.0
50	39297.0
51	38458.0
52	35537.5
53	32617.0
54	29586.0
55	26555.0
56	25149.5
57	23744.0
58	22594.0
59	21444.0
60	20802.5
61	20161.0
62	18301.5
63	16442.0
64	14295.0
65	12148.0
66	9980.5
67	7813.0
68	6665.5
69	5518.0
70	4724.0
71	3930.0
72	3244.5
73	2559.0
74	2158.5
75	1417.5
76	1077.0
77	798.0
78	519.0
79	430.5
80	342.0
81	272.0
82	202.0
83	154.5
84	107.0
85	85.5
86	64.0
87	59.0
88	54.0
89	35.0
90	16.0
91	12.5
92	9.0
93	5.5
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	476419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.410045141121635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.47318587986948	28.22108386307807
2	8.719595841908076	6.698401398000514
3	4.093709074370821	4.7171865102360755
4	2.602803035446118	3.998951283397352
5	1.8178266256299445	3.49114013745895
6	1.34884568621141	3.108553421747247
7	1.0162056144646219	2.732275246697317
8	0.7545900296982949	2.318706968300146
9	0.63794414774825	2.205311716127219
>10	5.46366112004693	39.75297420315841
>50	0.05522845347498836	1.405326888465976
>100	0.015310858389105248	1.09552907640687
>500	0.0010936327420789464	0.25455928692582946
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	691	0.1450403951143846	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	521	0.10935751932647524	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11628419521471646	0.0
2	0.0	0.0	0.0	0.5045978434949068	0.0
3	0.0	0.0	0.0	0.6859508122052227	0.0
4	0.0	0.0	0.0	0.9273349719469627	0.0
5	0.0	0.0	0.0	1.659463623407127	0.0
6	0.0	0.0	0.0	2.1541962012430234	0.0
7	0.0	0.0	0.0	2.5956143646663965	0.0
8	0.0	0.0	0.0	3.3346696920148022	0.0
9	0.0	0.0	0.0	3.641962222329504	0.0
10	0.0	0.0	0.0	4.501499730279439	0.0
11	0.0	0.0	0.0	5.483408512254969	0.0
12	0.0	0.0	0.0	6.381987284302263	0.0
13	0.0	0.0	0.0	6.778487004086739	0.0
14	0.0	0.0	0.0	6.964457756722549	0.0
15	0.0	0.0	0.0	7.246772273985714	0.0
16	0.0	0.0	0.0	7.840157508411713	0.0
17	0.0	0.0	0.0	8.469855316433643	0.0
18	0.0	0.0	0.0	9.161893207449745	0.0
19	0.0	0.0	0.0	9.551046452807297	0.0
20	0.0	0.0	0.0	9.928235439812434	0.0
21	0.0	0.0	0.0	10.40302758706097	0.0
22	2.0989926934064342E-4	0.0	0.0	10.901538351744998	0.0
23	2.0989926934064342E-4	0.0	0.0	11.412852971858804	0.0
24	2.0989926934064342E-4	0.0	0.0	11.810402187989984	0.0
25	4.1979853868128684E-4	0.0	0.0	12.16492205390633	0.0
26	4.1979853868128684E-4	0.0	0.0	12.506008366584876	0.0
27	4.1979853868128684E-4	0.0	0.0	12.841217499721884	0.0
28	4.1979853868128684E-4	0.0	0.0	13.193218574406142	0.0
29	4.1979853868128684E-4	0.0	0.0	13.548997835938533	0.0
30	4.1979853868128684E-4	0.0	0.0	13.945077757184327	0.0
31	4.1979853868128684E-4	0.0	0.0	14.317229161725288	0.0
32	4.1979853868128684E-4	0.0	0.0	14.67657671083647	0.0
33	4.1979853868128684E-4	0.0	0.0	15.06132207153787	0.0
34	4.1979853868128684E-4	0.0	0.0	15.422978512611797	0.0
35	4.1979853868128684E-4	0.0	0.0	15.815909944817482	0.0
36	4.1979853868128684E-4	0.0	0.0	16.166441724616355	0.0
37	4.1979853868128684E-4	0.0	0.0	16.520541791994024	0.0
38	4.1979853868128684E-4	0.0	0.0	16.89521198776707	0.0
39	4.1979853868128684E-4	0.0	0.0	17.26946238500144	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATT	20	7.029123E-4	45.000004	19
CGATTAC	20	7.029123E-4	45.000004	10
TTACGAC	20	7.029123E-4	45.000004	13
ACGTTAG	25	3.887355E-5	45.0	1
TACGGGT	25	3.887355E-5	45.0	4
CGCGACA	25	3.887355E-5	45.0	29
GCCGATA	25	3.887355E-5	45.0	9
CGAATAT	25	3.887355E-5	45.0	14
CTCGACA	25	3.887355E-5	45.0	18
CGGTCTA	25	3.887355E-5	45.0	31
AGTACGA	25	3.887355E-5	45.0	45
GCGATTA	70	0.0	41.785713	9
TTAATCG	55	6.002665E-11	40.909092	20
CGTTGAT	55	6.002665E-11	40.909092	25
TAATCGT	55	6.002665E-11	40.909092	21
CGCACGG	40	3.4525328E-7	39.375004	2
TCAACGG	40	3.4525328E-7	39.375004	2
ACGGTTA	40	3.4525328E-7	39.375004	34
AGGGCGA	375	0.0	38.4	6
ACAACGA	65	9.094947E-12	38.076927	13
>>END_MODULE