##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936111.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269435
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.712709930038784	31.0	31.0	34.0	30.0	34.0
2	31.828381613376138	31.0	31.0	34.0	30.0	34.0
3	31.79151557889658	31.0	31.0	34.0	30.0	34.0
4	35.570055857628	37.0	35.0	37.0	33.0	37.0
5	31.051281385120717	35.0	35.0	37.0	0.0	37.0
6	33.24577727466736	37.0	35.0	37.0	17.0	37.0
7	29.531141091543414	35.0	32.0	37.0	0.0	37.0
8	32.36605860411602	35.0	33.0	37.0	17.0	37.0
9	36.13873475977508	39.0	35.0	39.0	32.0	39.0
10	36.609994989515094	38.0	35.0	39.0	32.0	39.0
11	36.75036650769202	38.0	35.0	39.0	33.0	39.0
12	36.70682724961493	39.0	35.0	39.0	33.0	39.0
13	36.34549705865979	39.0	35.0	39.0	32.0	39.0
14	37.601206227847165	39.0	36.0	41.0	33.0	41.0
15	37.63013342735725	39.0	36.0	41.0	33.0	41.0
16	37.77063113552434	39.0	36.0	41.0	33.0	41.0
17	37.66335108653293	39.0	36.0	41.0	33.0	41.0
18	37.57170003897044	39.0	36.0	41.0	33.0	41.0
19	37.472778221092284	39.0	36.0	41.0	32.0	41.0
20	37.447046597509605	39.0	36.0	41.0	32.0	41.0
21	37.40771243528123	39.0	35.0	41.0	32.0	41.0
22	37.415332083805	39.0	35.0	41.0	32.0	41.0
23	37.44069256035779	39.0	35.0	41.0	33.0	41.0
24	37.34673668973964	39.0	35.0	41.0	32.0	41.0
25	37.260437582348246	39.0	35.0	41.0	32.0	41.0
26	37.00950878690593	39.0	35.0	40.0	32.0	41.0
27	37.013339024254456	39.0	35.0	40.0	32.0	41.0
28	36.85466624603337	39.0	35.0	40.0	31.0	41.0
29	36.80333661179876	39.0	35.0	40.0	31.0	41.0
30	36.72553305992169	39.0	35.0	40.0	31.0	41.0
31	36.460715942620666	39.0	35.0	40.0	30.0	41.0
32	36.41026592684692	39.0	35.0	40.0	30.0	41.0
33	36.275495017351126	38.0	35.0	40.0	30.0	41.0
34	36.065147438157624	38.0	35.0	40.0	30.0	41.0
35	36.06459071761278	38.0	35.0	40.0	30.0	41.0
36	35.66506578581105	38.0	35.0	40.0	27.0	41.0
37	35.60441293818546	38.0	35.0	40.0	27.0	41.0
38	35.37749364410711	38.0	34.0	40.0	26.0	41.0
39	35.23073097407538	38.0	34.0	40.0	26.0	41.0
40	35.124879841149074	38.0	34.0	40.0	25.0	41.0
41	34.83143244196188	38.0	34.0	40.0	23.0	41.0
42	34.86954181899159	38.0	34.0	40.0	24.0	41.0
43	34.58173956612912	38.0	33.0	40.0	23.0	41.0
44	34.32714383803144	38.0	33.0	40.0	22.0	41.0
45	34.222703063818734	37.0	33.0	40.0	22.0	41.0
46	33.899118525804	37.0	33.0	40.0	21.0	41.0
47	33.64528736058789	37.0	32.0	40.0	20.0	41.0
48	33.478093046560396	37.0	32.0	40.0	19.0	41.0
49	33.28684098205504	36.0	32.0	40.0	18.0	41.0
50	33.12786015179913	36.0	31.0	40.0	18.0	41.0
51	30.742049102752055	35.0	27.0	38.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	6.0
10	18.0
11	14.0
12	4.0
13	2.0
14	4.0
15	8.0
16	21.0
17	63.0
18	142.0
19	260.0
20	444.0
21	760.0
22	1223.0
23	1756.0
24	2389.0
25	3095.0
26	3833.0
27	4617.0
28	5324.0
29	6244.0
30	7612.0
31	9258.0
32	11651.0
33	15390.0
34	21375.0
35	25352.0
36	26125.0
37	37874.0
38	44528.0
39	40036.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.53786627572513	18.97080928609869	22.29368864475662	10.197635793419563
2	29.092731085419487	31.175979364225135	22.53567650824874	17.19561304210663
3	29.55072652031102	29.773785885278453	25.090652662052072	15.584834932358454
4	26.875127581791524	24.15907361701338	30.68792101991204	18.277877781283056
5	24.251489227457455	37.56713121903242	20.417911555662776	17.763467997847346
6	23.856959934678123	38.53842299626997	24.152392970475255	13.452224098576652
7	63.69402638855382	19.60881103048973	11.850353517545976	4.84680906341047
8	73.85863009631265	10.710932135765583	9.995731809156197	5.434705958765565
9	68.1875034795034	8.687067381743278	11.11436895726242	12.011060181490897
10	40.535565164139776	26.08792473138234	18.72214077606844	14.65436932840945
11	31.76313396552044	24.764043275743685	25.001206227847163	18.471616530888713
12	30.68346725555329	21.251136637779055	29.15768181565127	18.907714291016386
13	22.798077458385137	26.55000278360272	32.87360587896895	17.778313879043182
14	18.916250672703992	31.29882903112068	26.96346057490675	22.82145972126858
15	16.256239909440126	26.84320893722048	39.84374710041383	17.056804052925568
16	18.973036168278064	26.462040937517394	32.087887616679346	22.477035277525193
17	19.007181695028486	25.063187781839773	30.33421790042125	25.59541262271049
18	20.296917623916716	27.084825653682703	30.379126691038653	22.239130031361924
19	23.336240651734187	28.389778610796668	25.939094772394082	22.334885965075067
20	24.52873605878969	26.69066750793327	31.123647633009817	17.656948800267223
21	22.482231335943734	30.287082227624474	27.5283463544083	19.702340082023493
22	19.174569005511533	25.799914636183125	27.343143986490247	27.682372371815095
23	19.248427264460815	30.60626867333494	28.298847588472174	21.84645647373207
24	21.686863250876833	24.79521962625494	28.4320893722048	25.085827750663427
25	19.270696086254567	32.03184441516507	26.830218791174126	21.86724070740624
26	20.14994340007794	27.3928776884963	28.519308924230337	23.93786998719543
27	22.523057509232284	30.05288845176016	25.39350863844712	22.03054540056043
28	18.68873754337781	28.02828140367807	31.588694861469367	21.694286191474752
29	24.373596600293208	24.954070555050382	26.460556349397812	24.211776495258597
30	23.166255312041866	27.537625030155695	27.530202089557775	21.76591756824466
31	23.293558743296156	28.929426392265295	24.164269675431925	23.612745189006628
32	24.156846734834005	28.359344554345206	24.9073060292835	22.576502681537292
33	21.685007515727357	27.660103550021343	23.940839163434593	26.71404977081671
34	22.543470595876556	26.585632898472728	28.248000445376437	22.62289606027428
35	22.350474140330693	23.996140070889084	28.067251841817136	25.58613394696309
36	23.427542821088572	31.66292426744855	25.217584946276467	19.69194796518641
37	21.661254105814017	27.813016126338447	29.745949858036262	20.77977990981127
38	18.87134188208659	28.294764971143316	26.32805685972498	26.505836287045113
39	22.179004212518787	24.847551357470262	28.741625995137976	24.231818434872977
40	22.060979457011896	23.096479670421438	32.220387106352185	22.62215376621449
41	18.228144079277005	29.194425371610965	29.17438343199659	23.403047117115445
42	20.47284131608737	25.215358064097092	32.42117764952586	21.89062297028968
43	23.341807857182623	26.728524504982648	25.81735854658823	24.112309091246498
44	20.778666468721585	26.271271364150913	27.617421641583313	25.332640525544193
45	20.285783213019837	24.985989199621432	27.14235344331657	27.58587414404216
46	23.718150945497058	28.691521146102026	25.7928628426151	21.79746506578581
47	19.7828789875109	24.801900272793066	33.38764451537477	22.027576224321265
48	21.46603076808878	25.37161096368326	28.13888321858704	25.023475049640915
49	19.371648078386254	23.201885426911872	32.845769851726764	24.580696642975113
50	19.84820086477258	24.27041772598215	30.16237682557945	25.719004583665818
51	20.310278916992967	23.569320986508803	27.14124000222688	28.979160094271343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	457.0
1	537.0
2	617.0
3	798.5
4	980.0
5	804.0
6	628.0
7	566.5
8	505.0
9	509.5
10	514.0
11	513.5
12	513.0
13	555.0
14	597.0
15	654.0
16	711.0
17	658.5
18	606.0
19	756.5
20	907.0
21	962.0
22	1017.0
23	1158.5
24	1300.0
25	1714.5
26	2578.5
27	3028.0
28	3533.5
29	4039.0
30	4719.0
31	5399.0
32	5946.5
33	6494.0
34	6846.5
35	7199.0
36	7967.0
37	8735.0
38	9387.0
39	10039.0
40	11704.5
41	13370.0
42	14826.0
43	16282.0
44	19652.5
45	23023.0
46	29299.5
47	35576.0
48	30254.0
49	24932.0
50	23557.0
51	22182.0
52	19482.0
53	16782.0
54	14985.5
55	13189.0
56	11864.0
57	10539.0
58	10344.0
59	10149.0
60	9385.5
61	8622.0
62	7610.0
63	6598.0
64	5425.5
65	4253.0
66	3634.0
67	3015.0
68	2405.5
69	1796.0
70	1462.0
71	1128.0
72	884.0
73	640.0
74	501.0
75	305.0
76	248.0
77	209.5
78	171.0
79	113.0
80	55.0
81	52.0
82	49.0
83	41.5
84	34.0
85	23.0
86	12.0
87	10.0
88	8.0
89	5.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	269435.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.71405752732509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.55352226940593	28.11715827733088
2	9.251622982515165	6.978324827673996
3	3.978513474335694	4.501376581330031
4	2.3253224093186793	3.5078937245849136
5	1.6605003080470346	3.1312102070913443
6	1.2076359390484661	2.7326910766403465
7	0.9037275296795855	2.3858262430354293
8	0.6937908235925611	2.0932533626320082
9	0.5328393500566064	1.8085980510771629
>10	4.772561082843745	35.52121258770239
>50	0.07697679165876767	1.9318858481688936
>100	0.0349894507539853	2.929122005156935
>500	0.004998492964855043	1.1944212269709318
>1k	0.002999095778913026	3.167025980604744
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	3191	1.1843301723977955	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	2817	1.0455211832167313	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	2392	0.8877836955109767	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	804	0.2984022120362982	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCT	668	0.24792621597045672	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	664	0.24644162785087315	No Hit
GAATATGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	526	0.19522333772523986	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTC	506	0.187800397127322	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	493	0.18297548573867536	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTT	477	0.1770371332603411	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	459	0.170356486722215	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	419	0.15551060552637927	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT	353	0.13101490155325032	No Hit
GAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	344	0.1276745782841873	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	339	0.12581884313470781	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	297	0.1102306678790803	No Hit
GAATCTATCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	288	0.10689034461001726	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTA	275	0.10206543322137064	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17295451593148625	0.0
2	0.0	0.0	0.0	1.0655631228311095	0.0
3	0.0	0.0	0.0	1.3253660437582349	0.0
4	0.0	0.0	0.0	1.8123109469816467	0.0
5	0.0	0.0	0.0	4.210663054168909	0.0
6	0.0	0.0	0.0	4.937368938705068	0.0
7	0.0	0.0	0.0	5.923506597138457	0.0
8	0.0	0.0	0.0	7.176127823037096	0.0
9	0.0	0.0	0.0	7.548759441052573	0.0
10	0.0	0.0	0.0	10.547256295581494	0.0
11	0.0	0.0	0.0	11.74791693729471	0.0
12	0.0	0.0	0.0	14.387143466884407	0.0
13	0.0	0.0	0.0	14.891903427542822	0.0
14	0.0	0.0	0.0	15.147252584111195	0.0
15	0.0	0.0	0.0	15.644218457141797	0.0
16	0.0	0.0	0.0	16.34457290255535	0.0
17	0.0	0.0	0.0	17.10690890196151	0.0
18	0.0	0.0	0.0	18.003971273219886	0.0
19	0.0	0.0	0.0	18.482008647725795	0.0
20	0.0	0.0	0.0	18.988995490563585	0.0
21	0.0	0.0	0.0	19.564644533932118	0.0
22	0.0	0.0	0.0	20.17443910405107	0.0
23	0.0	0.0	0.0	20.79425464397721	0.0
24	0.0	0.0	0.0	21.27080743036354	0.0
25	0.0	0.0	0.0	21.694657338504648	0.0
26	0.0	0.0	0.0	22.067660103550022	0.0
27	0.0	0.0	0.0	22.458849073060293	0.0
28	0.0	0.0	0.0	22.87490489357359	0.0
29	0.0	0.0	0.0	23.28613580269824	0.0
30	0.0	0.0	0.0	23.77122497077217	0.0
31	0.0	0.0	0.0	24.218828288826618	0.0
32	0.0	0.0	0.0	24.645276226177	0.0
33	0.0	0.0	0.0	25.02310390261102	0.0
34	0.0	0.0	0.0	25.458459368678902	0.0
35	0.0	0.0	0.0	25.909031862972515	0.0
36	0.0	0.0	0.0	26.337335535472377	0.0
37	0.0	0.0	0.0	26.79161950006495	0.0
38	0.0	0.0	0.0	27.202479262159706	0.0
39	0.0	0.0	0.0	27.621504258912168	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	50	2.1827873E-11	45.000004	2
GCGTTGG	25	3.8828293E-5	45.000004	2
CGGGAGC	25	3.8828293E-5	45.000004	6
CGATACA	25	3.8828293E-5	45.000004	13
TCTATCT	95	0.0	45.000004	4
TAATACG	80	0.0	45.0	4
CGAGTGA	20	7.023662E-4	45.0	14
CGTGCGG	20	7.023662E-4	45.0	2
CGGCATC	20	7.023662E-4	45.0	41
ACCGACC	20	7.023662E-4	45.0	30
ACGGGTG	40	6.7811925E-9	45.0	5
ACGGGAC	35	1.2072996E-7	45.0	5
CATAGCG	20	7.023662E-4	45.0	1
GTGTACG	20	7.023662E-4	45.0	1
AGCGAGT	20	7.023662E-4	45.0	12
GCGATAT	35	1.2072996E-7	45.0	9
GGTTGAC	20	7.023662E-4	45.0	8
GTTGGCA	20	7.023662E-4	45.0	29
AGAGCGA	20	7.023662E-4	45.0	10
ACGACTC	20	7.023662E-4	45.0	20
>>END_MODULE