##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936110.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	832217
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89810710427689	31.0	31.0	34.0	30.0	34.0
2	32.0275565147071	33.0	31.0	34.0	30.0	34.0
3	31.97305149978912	33.0	31.0	34.0	30.0	34.0
4	35.69627032372566	37.0	35.0	37.0	35.0	37.0
5	31.101990226106892	37.0	35.0	37.0	0.0	37.0
6	33.345254903468685	37.0	35.0	37.0	17.0	37.0
7	29.5851995332948	35.0	32.0	37.0	0.0	37.0
8	32.54774295646448	35.0	33.0	37.0	17.0	37.0
9	36.39184010901003	39.0	35.0	39.0	32.0	39.0
10	36.75981745145797	38.0	35.0	39.0	33.0	39.0
11	36.90883387385742	39.0	37.0	39.0	33.0	39.0
12	36.95265057070451	39.0	37.0	39.0	33.0	39.0
13	36.763180756942	39.0	35.0	39.0	33.0	39.0
14	38.04098209962065	40.0	37.0	41.0	33.0	41.0
15	38.09869300915506	40.0	37.0	41.0	33.0	41.0
16	38.14278727783739	40.0	37.0	41.0	33.0	41.0
17	38.069407378123735	40.0	37.0	41.0	33.0	41.0
18	37.97420144024936	40.0	37.0	41.0	33.0	41.0
19	37.89210987038236	40.0	37.0	41.0	33.0	41.0
20	37.857933687968405	40.0	36.0	41.0	33.0	41.0
21	37.79913772489627	40.0	36.0	41.0	33.0	41.0
22	37.79667682827916	40.0	36.0	41.0	33.0	41.0
23	37.76413964146371	40.0	36.0	41.0	33.0	41.0
24	37.70476570413726	40.0	36.0	41.0	33.0	41.0
25	37.595323094817815	40.0	36.0	41.0	33.0	41.0
26	37.41727097619972	39.0	36.0	41.0	32.0	41.0
27	37.364460230925346	39.0	36.0	41.0	32.0	41.0
28	37.239101099833334	39.0	36.0	41.0	32.0	41.0
29	37.22111300297879	39.0	36.0	41.0	32.0	41.0
30	37.09608070971874	39.0	35.0	41.0	31.0	41.0
31	36.964510458209816	39.0	35.0	41.0	31.0	41.0
32	36.90807085171296	39.0	35.0	41.0	31.0	41.0
33	36.75837191501736	39.0	35.0	41.0	31.0	41.0
34	36.6101425469559	39.0	35.0	41.0	30.0	41.0
35	36.53422124277682	39.0	35.0	41.0	30.0	41.0
36	36.40450507499847	39.0	35.0	40.0	30.0	41.0
37	36.339044984661456	39.0	35.0	40.0	30.0	41.0
38	36.21620202423166	39.0	35.0	40.0	30.0	41.0
39	36.242437969904486	39.0	35.0	40.0	30.0	41.0
40	36.1012692603011	39.0	35.0	40.0	29.0	41.0
41	35.98040895583724	39.0	35.0	40.0	29.0	41.0
42	35.952056975524414	39.0	35.0	40.0	29.0	41.0
43	35.79298428174383	38.0	35.0	40.0	28.0	41.0
44	35.63902083230696	38.0	35.0	40.0	27.0	41.0
45	35.51999899064787	38.0	34.0	40.0	27.0	41.0
46	35.38140533058085	38.0	34.0	40.0	26.0	41.0
47	35.220223811818315	38.0	34.0	40.0	26.0	41.0
48	35.10910255378104	38.0	34.0	40.0	26.0	41.0
49	34.952513587201416	38.0	34.0	40.0	25.0	41.0
50	34.804328678697985	38.0	34.0	40.0	24.0	41.0
51	32.74331574577304	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	17.0
9	42.0
10	48.0
11	28.0
12	31.0
13	15.0
14	33.0
15	47.0
16	105.0
17	191.0
18	384.0
19	678.0
20	1125.0
21	1748.0
22	2530.0
23	3711.0
24	5110.0
25	6658.0
26	8344.0
27	10210.0
28	12050.0
29	14600.0
30	18082.0
31	23009.0
32	29764.0
33	42032.0
34	58890.0
35	70484.0
36	82352.0
37	127620.0
38	158466.0
39	153793.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35645991370039	19.98781567788209	24.455280293481145	11.200444114936369
2	30.655946706207637	27.0472725262762	25.38460521714889	16.912175550367273
3	31.834485476744646	26.478550666472806	25.55331121570456	16.13365264107799
4	28.63039327483096	25.60486027081879	27.182693936797737	18.582052517552512
5	23.432830619898414	39.415561085630316	20.29242373082982	16.859184563641456
6	25.458864695145618	36.4558762918806	23.7096814893231	14.375577523650682
7	68.47480885394074	19.558360379564466	7.924495654378605	4.0423351121161915
8	81.99003384934458	6.830910687957588	7.478698464462995	3.7003569982348354
9	76.06201267217565	8.01329460945883	9.131512574244457	6.793180144121065
10	39.724735255348065	29.03449460897819	17.021161548009715	14.219608587664034
11	29.86552786112276	26.214316698649508	25.958734320495736	17.961421119731995
12	29.2454972681404	23.66798563355471	28.504945224622908	18.581571873681984
13	24.857338891178625	24.54287763888505	30.198974546302225	20.400808923634102
14	20.255053669896192	28.40220759729734	28.51660083848323	22.826137894323235
15	19.856720062195315	27.040903994991687	33.10158288042662	20.000793062386375
16	23.14276204403419	26.066518708461857	29.883912489170495	20.906806758333463
17	22.367964124741505	26.032032510751403	28.415305142769252	23.18469822173784
18	22.96083833903898	27.50712854940478	28.846322533666097	20.68571057789014
19	24.488444720547644	28.41506482083399	26.38446462881676	20.712025829801604
20	25.328129562361738	27.129222306201388	28.50398393688185	19.03866419455503
21	24.699086896806964	28.395478583109934	27.530199455190175	19.37523506489293
22	23.29416486325081	25.08672617838857	27.70984010180037	23.90926885656025
23	22.81135809530447	27.735314226938407	28.404971299552884	21.048356378204243
24	22.860864413968955	25.256633786620558	29.23948921975879	22.6430125796517
25	22.118149473034077	28.553249933611063	26.93684459702217	22.391755996332687
26	21.507851918429928	27.77905281915654	27.338182228913855	23.374913033499674
27	22.152755831712163	28.221365341010817	27.5198656119738	22.106013215303218
28	19.78786782774204	28.239389486155652	29.640947012618103	22.3317956734842
29	21.750577073047054	26.69820491530454	27.919040346448103	23.632177665200302
30	22.63448115094981	26.986951720524814	28.51323633138953	21.865330797135844
31	22.80282666660258	27.898733142918253	26.66203646404724	22.63640372643193
32	23.50444655660723	27.805488232035636	26.757203950411974	21.932861260945163
33	23.118609689540108	27.155898041015746	27.575379979019893	22.150112290424254
34	21.768721379159523	27.272334018651385	28.79549444435766	22.16345015783143
35	22.483318653668455	25.927131986008455	28.60672156420741	22.982827796115675
36	23.25667464134955	29.439797552801732	26.726683064633384	20.576844741215332
37	22.387790684400823	29.379236425115085	27.56168162870982	20.67129126177427
38	21.267770305100715	28.6774963741428	27.533924445186774	22.520808875569713
39	22.93308115551593	27.343949955360202	27.18317458066826	22.53979430845561
40	22.199137965218206	26.14402253258465	30.175062513743413	21.48177698845373
41	20.898876134469734	28.000869965405656	28.397641480527312	22.702612419597294
42	21.708761056311037	26.729326605921294	28.595186111314717	22.96672622645296
43	22.962881075488724	26.348536499494724	27.064695866582873	23.623886558433675
44	21.81570431750373	26.68787107208817	27.385886133063853	24.11053847734425
45	21.33842495406847	26.43361046457835	27.202400335489425	25.02556424586376
46	21.69986914470625	28.44150023371308	26.717550831093334	23.141079790487336
47	20.91882285509669	26.357188089164246	30.257372776571493	22.466616279167575
48	21.204685797093788	26.1091758519713	29.31783417065501	23.368304180279903
49	21.077915976241773	26.14402253258465	29.603576951684474	23.1744845394891
50	20.332917976921884	25.798319428706694	29.99001462358976	23.878747970781657
51	20.473986952922136	24.66508134296704	28.757523578585875	26.10340812552495
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	596.0
1	1189.0
2	1782.0
3	2814.0
4	3846.0
5	2980.0
6	2114.0
7	1760.5
8	1407.0
9	1409.0
10	1411.0
11	1394.5
12	1378.0
13	1433.0
14	1488.0
15	1505.0
16	1522.0
17	1656.5
18	1791.0
19	2012.5
20	2234.0
21	2819.0
22	3404.0
23	3829.0
24	4254.0
25	5548.5
26	8623.5
27	10404.0
28	12127.0
29	13850.0
30	16434.5
31	19019.0
32	21700.0
33	24381.0
34	26319.0
35	28257.0
36	30141.5
37	32026.0
38	35404.5
39	38783.0
40	40536.0
41	42289.0
42	45842.5
43	49396.0
44	54516.5
45	59637.0
46	68034.0
47	76431.0
48	70403.5
49	64376.0
50	61596.0
51	58816.0
52	54598.0
53	50380.0
54	46318.5
55	42257.0
56	40072.5
57	37888.0
58	37022.5
59	36157.0
60	35491.0
61	34825.0
62	31220.0
63	27615.0
64	22913.5
65	18212.0
66	15297.5
67	12383.0
68	10077.5
69	7772.0
70	6493.5
71	5215.0
72	4334.5
73	3454.0
74	2621.5
75	1419.0
76	1049.0
77	858.0
78	667.0
79	518.5
80	370.0
81	268.5
82	167.0
83	142.5
84	118.0
85	102.5
86	87.0
87	63.0
88	39.0
89	27.0
90	15.0
91	12.5
92	10.0
93	7.5
94	5.0
95	5.5
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	832217.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.456533162033146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.45456799600734	24.57535190061238
2	8.410933648395256	5.628013610623926
3	3.6895533978046178	3.703189968202268
4	2.3148405889745436	3.097861637193886
5	1.6985677997351203	2.841409495989986
6	1.3090077755121947	2.6276917230469787
7	1.051933024695538	2.463582248747487
8	0.8194090790027629	2.1931669619941583
9	0.6312762251384356	1.9008282564672456
>10	6.477414803099554	44.72804260681743
>50	0.10779464844586428	2.306018867662014
>100	0.03027109661150273	2.008364277374866
>500	0.002953277718195337	0.6163051315479056
>1k	0.0014766388590976686	1.3101733137194753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	3516	0.4224859621949564	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	2857	0.34329988452531013	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	2757	0.3312837877620861	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1478	0.17759791016045093	No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	964	0.1158351727974795	RNA PCR Primer, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2016096763224015E-4	0.0	0.0	0.06392563478035176	0.0
2	1.2016096763224015E-4	0.0	0.0	0.36312644418462975	0.0
3	1.2016096763224015E-4	0.0	0.0	0.4711511540860136	0.0
4	1.2016096763224015E-4	0.0	0.0	0.6940497490438191	0.0
5	1.2016096763224015E-4	0.0	0.0	1.59417555757693	0.0
6	1.2016096763224015E-4	0.0	0.0	1.9806132294822143	0.0
7	1.2016096763224015E-4	0.0	0.0	2.39552905071634	0.0
8	1.2016096763224015E-4	0.0	0.0	3.0081096637055	0.0
9	1.2016096763224015E-4	0.0	0.0	3.2219961860908874	0.0
10	1.2016096763224015E-4	0.0	0.0	4.321469039925884	0.0
11	1.2016096763224015E-4	0.0	0.0	4.950271383545397	0.0
12	1.2016096763224015E-4	0.0	0.0	5.9166058852438725	0.0
13	1.2016096763224015E-4	0.0	0.0	6.161854420181275	0.0
14	1.2016096763224015E-4	0.0	0.0	6.27372428104689	0.0
15	1.2016096763224015E-4	0.0	0.0	6.500468026968927	0.0
16	1.2016096763224015E-4	0.0	0.0	6.9185080333614914	0.0
17	1.2016096763224015E-4	0.0	0.0	7.392422889703046	0.0
18	1.2016096763224015E-4	0.0	0.0	7.908874728586414	0.0
19	1.2016096763224015E-4	0.0	0.0	8.219370668948123	0.0
20	1.2016096763224015E-4	0.0	0.0	8.519773088028723	0.0
21	1.2016096763224015E-4	0.0	0.0	8.889268063497862	0.0
22	1.2016096763224015E-4	0.0	0.0	9.294571007321407	0.0
23	1.2016096763224015E-4	0.0	0.0	9.715855359840042	0.0
24	1.2016096763224015E-4	0.0	0.0	10.043293996637896	0.0
25	1.2016096763224015E-4	0.0	0.0	10.339490781851367	0.0
26	1.2016096763224015E-4	0.0	0.0	10.629078713845066	0.0
27	1.2016096763224015E-4	0.0	0.0	10.903045720046574	0.0
28	1.2016096763224015E-4	0.0	0.0	11.201886046547955	0.0
29	1.2016096763224015E-4	0.0	0.0	11.508176353042536	0.0
30	1.2016096763224015E-4	0.0	0.0	11.862290724654747	0.0
31	2.403219352644803E-4	0.0	0.0	12.172185860178296	0.0
32	2.403219352644803E-4	0.0	0.0	12.494938219238492	0.0
33	2.403219352644803E-4	0.0	0.0	12.813364783463928	0.0
34	3.6048290289672047E-4	0.0	0.0	13.127705874789868	0.0
35	3.6048290289672047E-4	0.0	0.0	13.500805679287975	0.0
36	3.6048290289672047E-4	0.0	0.0	13.82247658963948	0.0
37	3.6048290289672047E-4	0.0	0.0	14.163253093844514	0.0
38	3.6048290289672047E-4	0.0	0.0	14.515324729006977	0.0
39	3.6048290289672047E-4	0.0	0.0	14.871722159004202	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	35	1.2113924E-7	45.0	1
ATTACGG	55	1.8189894E-12	45.0	2
TACGGGT	135	0.0	45.0	4
TACGCTT	25	3.8898746E-5	45.0	18
GATTACG	25	3.8898746E-5	45.0	1
TCGAACG	35	1.2113924E-7	45.0	1
ACGGGTA	170	0.0	42.35294	5
CGGTCTA	50	1.0804797E-9	40.5	31
TACGGCT	520	0.0	39.80769	7
TAATACG	125	0.0	39.6	4
CACAACG	120	0.0	39.374996	12
CGATTAG	75	0.0	39.0	10
AGATCGG	35	6.2462113E-6	38.571426	2
AACGCCA	35	6.2462113E-6	38.571426	15
GTTCACG	35	6.2462113E-6	38.571426	1
ACGGCTG	650	0.0	38.423073	8
TCGTAAG	65	9.094947E-12	38.07692	1
TCGCTAC	30	1.1396512E-4	37.499996	41
CTTTCGT	30	1.1396512E-4	37.499996	15
CAATACG	30	1.1396512E-4	37.499996	32
>>END_MODULE