##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936109.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	450864
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87062839348451	31.0	31.0	34.0	30.0	34.0
2	32.00233773377338	33.0	31.0	34.0	30.0	34.0
3	31.924773324106603	33.0	31.0	34.0	30.0	34.0
4	35.65602044075375	37.0	35.0	37.0	33.0	37.0
5	31.132714077859397	37.0	35.0	37.0	0.0	37.0
6	33.35713430213989	37.0	35.0	37.0	17.0	37.0
7	29.47376148905213	35.0	32.0	37.0	0.0	37.0
8	32.53316742964619	35.0	33.0	37.0	17.0	37.0
9	36.31930249476561	39.0	35.0	39.0	32.0	39.0
10	36.731422335781964	38.0	35.0	39.0	33.0	39.0
11	36.896849160722525	39.0	37.0	39.0	33.0	39.0
12	36.84826910110366	39.0	35.0	39.0	33.0	39.0
13	36.62448321445048	39.0	35.0	39.0	33.0	39.0
14	37.86322926647504	40.0	37.0	41.0	33.0	41.0
15	37.964080077362574	40.0	37.0	41.0	33.0	41.0
16	38.024235689698	40.0	37.0	41.0	33.0	41.0
17	37.954573884807836	40.0	36.0	41.0	33.0	41.0
18	37.86905807516236	40.0	36.0	41.0	33.0	41.0
19	37.80360596543525	40.0	36.0	41.0	33.0	41.0
20	37.74539550729266	40.0	36.0	41.0	33.0	41.0
21	37.620255775577554	39.0	36.0	41.0	33.0	41.0
22	37.57754888392065	39.0	36.0	41.0	33.0	41.0
23	37.53906721317293	39.0	36.0	41.0	33.0	41.0
24	37.498250026615565	39.0	35.0	41.0	33.0	41.0
25	37.33412292842188	39.0	35.0	41.0	32.0	41.0
26	37.17737055963661	39.0	35.0	41.0	32.0	41.0
27	37.11135065119415	39.0	35.0	41.0	32.0	41.0
28	36.808875847262144	39.0	35.0	41.0	31.0	41.0
29	36.71984900102913	39.0	35.0	41.0	31.0	41.0
30	36.58904902587033	39.0	35.0	41.0	30.0	41.0
31	36.46847829944285	39.0	35.0	41.0	30.0	41.0
32	36.430029898151105	39.0	35.0	40.0	30.0	41.0
33	36.23055511196281	39.0	35.0	40.0	30.0	41.0
34	36.10024087086128	39.0	35.0	40.0	29.0	41.0
35	36.02288716774903	39.0	35.0	40.0	29.0	41.0
36	35.88438198658576	38.0	35.0	40.0	28.0	41.0
37	35.80972976329891	38.0	35.0	40.0	28.0	41.0
38	35.65950707974023	38.0	35.0	40.0	27.0	41.0
39	35.651722027041416	38.0	35.0	40.0	27.0	41.0
40	35.53413446183328	38.0	35.0	40.0	26.0	41.0
41	35.403939990773274	38.0	34.0	40.0	26.0	41.0
42	35.36417855495227	38.0	34.0	40.0	26.0	41.0
43	35.15026038894212	38.0	34.0	40.0	25.0	41.0
44	35.024297792682496	38.0	34.0	40.0	25.0	41.0
45	34.897423613329074	38.0	34.0	40.0	24.0	41.0
46	34.73573183931296	38.0	34.0	40.0	24.0	41.0
47	34.6356728414777	37.0	33.0	40.0	23.0	41.0
48	34.555471272933744	37.0	33.0	40.0	23.0	41.0
49	34.282925671599415	37.0	33.0	40.0	23.0	41.0
50	34.10184445863941	37.0	33.0	40.0	22.0	41.0
51	32.0207823201675	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	15.0
10	25.0
11	23.0
12	14.0
13	15.0
14	17.0
15	33.0
16	66.0
17	149.0
18	299.0
19	606.0
20	1129.0
21	1728.0
22	2323.0
23	2945.0
24	3650.0
25	4532.0
26	5349.0
27	6199.0
28	7158.0
29	8505.0
30	10258.0
31	12815.0
32	16616.0
33	23490.0
34	33755.0
35	40360.0
36	44782.0
37	67929.0
38	81334.0
39	74730.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.441844990950706	20.572279002093758	25.023510415557688	11.96236559139785
2	32.62890805209553	24.84562972426275	24.48875935980695	18.03670286383477
3	32.84737747968345	24.059139784946236	26.109425458674902	16.98405727669541
4	28.860144078924023	25.94219099329288	25.455791546896627	19.741873380886478
5	21.947416515845134	39.47953263068242	21.226356506618405	17.34669434685404
6	26.18483622555804	35.574363888001706	23.832242095177257	14.408557791262997
7	68.87176620887895	20.092533446893075	6.898532595195003	4.137167749032968
8	82.19440895702473	6.138880017033962	7.753779410199084	3.912931615742219
9	74.83210014549843	9.070806274175805	9.986381702686398	6.110711877639377
10	40.0617481102949	29.911458887824267	16.377000603286135	13.649792398594698
11	31.37287519074488	27.240365165548813	23.970864828418325	17.41589481528798
12	29.297970119592602	23.64061002874481	28.692687817168817	18.368732034493775
13	25.290331452500087	26.167314312076368	28.70732637779907	19.83502785762447
14	20.457832073529932	29.83693530643387	29.09436104900813	20.61087157102807
15	19.630975194293622	26.10365875297207	34.061934419248374	20.20343163348593
16	22.649402036977893	25.407661733915326	31.67873238936797	20.264203839738812
17	22.428049256538557	24.335941658681996	28.158158557791264	25.077850526988183
18	24.810364100926222	25.27325313176479	28.803364207388483	21.11301855992051
19	25.485734057276694	26.803426310372974	26.457202171830087	21.253637460520245
20	26.633308492139534	25.804455445544555	26.684099861599066	20.878136200716845
21	25.531867702899326	27.302911742787185	27.464379502466375	19.70084105184712
22	23.523723340075943	25.453351786791583	27.252563966074028	23.770360907058446
23	22.74499627382093	26.35362326555236	27.89932219028354	23.002058270343163
24	21.946307533979205	24.983808864757442	29.778381063912846	23.29150253735051
25	22.19383228645445	27.046071542638135	27.312892579580538	23.447203591326872
26	20.997240853117567	28.006228042159055	26.830707264274817	24.16582384044856
27	22.098016253238224	27.654015401540157	27.526482486958376	22.721485858263247
28	20.01845345824905	28.662745306788747	29.033588842755243	22.28521239220696
29	21.23899889988999	27.06958195819582	28.713758472621453	22.977660669292735
30	22.71971148727776	26.227864721956067	28.924021434401503	22.128402356364667
31	23.38842755243266	28.400360197310054	25.932653749245894	22.278558501011393
32	23.824701018488945	27.963199545761025	26.38334397955925	21.82875545619078
33	22.915335888427553	28.271274708115975	26.152897547819297	22.660491855637176
34	20.68539515241847	28.220705135029633	28.84129138720324	22.252608325348664
35	20.413029206146422	27.196893076404415	28.112690301288197	24.27738741616097
36	21.394256361119986	30.48258454877746	26.633530288512723	21.489628801589834
37	21.3971397139714	29.87929841371234	26.64905603463572	22.07450583768054
38	20.86859895666986	29.17997444905781	25.787598921182443	24.16382767308989
39	23.176390219667127	26.391994038113488	26.91720784981724	23.514407892402144
40	23.422584193903262	25.956385961176764	28.48419922637425	22.136830618545726
41	19.916427126583628	26.91876042442954	27.997799779978	25.167012669008837
42	22.41695943787927	27.024779090812306	26.686761418077293	23.871500053231127
43	22.147033251712266	26.59271975584655	27.029436814649205	24.230810177791973
44	21.349231697363287	27.321099045388408	27.023891905319562	24.305777351928743
45	21.15737783455765	26.663029206146422	26.380238830334648	25.799354128961284
46	21.612947585081088	28.0102203768764	26.78302104403989	23.593810994002627
47	21.0728734163739	26.63996238333511	28.278372192057915	24.008792008233083
48	21.33459313673303	26.11829731360233	28.224919266120164	24.322190283544483
49	21.178226693637107	26.59893005429575	28.42231803825544	23.800525213811703
50	19.70860392490862	26.39909152205543	29.10966499875794	24.782639554278006
51	20.44252812378012	25.826635082863124	27.2312715142482	26.499565279108555
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	488.0
1	922.0
2	1356.0
3	1788.5
4	2221.0
5	1719.0
6	1217.0
7	1018.5
8	820.0
9	840.0
10	860.0
11	929.5
12	999.0
13	1006.5
14	1014.0
15	1067.5
16	1121.0
17	1305.5
18	1490.0
19	1433.5
20	1377.0
21	1560.0
22	1743.0
23	2151.5
24	2560.0
25	3183.5
26	4199.5
27	4592.0
28	5220.0
29	5848.0
30	7605.0
31	9362.0
32	10635.0
33	11908.0
34	12538.0
35	13168.0
36	13697.0
37	14226.0
38	15957.5
39	17689.0
40	20780.0
41	23871.0
42	26713.0
43	29555.0
44	33000.0
45	36445.0
46	37734.0
47	39023.0
48	36881.0
49	34739.0
50	32305.0
51	29871.0
52	27476.0
53	25081.0
54	23860.5
55	22640.0
56	22296.0
57	21952.0
58	21603.0
59	21254.0
60	20026.0
61	18798.0
62	17093.5
63	15389.0
64	13186.5
65	10984.0
66	9290.0
67	7596.0
68	6475.0
69	5354.0
70	4589.0
71	3824.0
72	3186.5
73	2549.0
74	2121.5
75	1366.5
76	1039.0
77	812.0
78	585.0
79	434.5
80	284.0
81	236.5
82	189.0
83	157.5
84	126.0
85	97.5
86	69.0
87	61.0
88	53.0
89	36.5
90	20.0
91	15.0
92	10.0
93	6.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	450864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.26272668184038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19035473758055	28.387252657528254
2	8.554503240846216	6.546372388068329
3	3.8909727937962617	4.4663768560650965
4	2.507133848389993	3.8371910878294773
5	1.720212020393128	3.2910001185559348
6	1.3141645376897375	3.0170111112353726
7	0.9786721661564061	2.6212665923337064
8	0.7454412706713283	2.281809247764866
9	0.5600178037249964	1.928502734480466
>10	5.44190429753083	40.197339209964646
>50	0.07405856438460504	1.7996098372146996
>100	0.0219861363016796	1.4655452235651745
>500	5.785825342547263E-4	0.16072293539400553
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	726	0.16102416693282232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2179637318570566E-4	0.0	0.0	0.06454274459704035	0.0
2	2.2179637318570566E-4	0.0	0.0	0.3455587494233294	0.0
3	2.2179637318570566E-4	0.0	0.0	0.5043649526242947	0.0
4	2.2179637318570566E-4	0.0	0.0	0.7093048014478868	0.0
5	2.2179637318570566E-4	0.0	0.0	1.368483622555804	0.0
6	2.2179637318570566E-4	0.0	0.0	1.8284893005429574	0.0
7	2.2179637318570566E-4	0.0	0.0	2.2186291209766136	0.0
8	2.2179637318570566E-4	0.0	0.0	2.934366017246886	0.0
9	2.2179637318570566E-4	0.0	0.0	3.228911600837503	0.0
10	2.2179637318570566E-4	0.0	0.0	3.940434366017247	0.0
11	2.2179637318570566E-4	0.0	0.0	4.795015791901771	0.0
12	2.2179637318570566E-4	0.0	0.0	5.5253912488023	0.0
13	2.2179637318570566E-4	0.0	0.0	5.937488910181341	0.0
14	2.2179637318570566E-4	0.0	0.0	6.14154157351219	0.0
15	2.2179637318570566E-4	0.0	0.0	6.402152312005394	0.0
16	2.2179637318570566E-4	0.0	0.0	6.9151673231839315	0.0
17	2.2179637318570566E-4	0.0	0.0	7.474759572731466	0.0
18	2.2179637318570566E-4	0.0	0.0	8.137708932183541	0.0
19	2.2179637318570566E-4	0.0	0.0	8.52407821427304	0.0
20	4.435927463714113E-4	0.0	0.0	8.92952198445651	0.0
21	4.435927463714113E-4	0.0	0.0	9.38664430959225	0.0
22	4.435927463714113E-4	0.0	0.0	9.852194896909046	0.0
23	4.435927463714113E-4	0.0	0.0	10.302441534476028	0.0
24	4.435927463714113E-4	0.0	0.0	10.667296568366513	0.0
25	4.435927463714113E-4	0.0	0.0	10.991784662337201	0.0
26	4.435927463714113E-4	0.0	0.0	11.293871322616132	0.0
27	4.435927463714113E-4	0.0	0.0	11.610152950778948	0.0
28	4.435927463714113E-4	0.0	0.0	11.947727030767593	0.0
29	4.435927463714113E-4	0.0	0.0	12.292176798324993	0.0
30	4.435927463714113E-4	0.0	0.0	12.694959012030235	0.0
31	4.435927463714113E-4	0.0	0.0	13.07689236665602	0.0
32	4.435927463714113E-4	0.0	0.0	13.420454948720678	0.0
33	4.435927463714113E-4	0.0	0.0	13.779765073281522	0.0
34	4.435927463714113E-4	0.0	0.0	14.139740586961922	0.0
35	4.435927463714113E-4	0.0	0.0	14.51945597785585	0.0
36	4.435927463714113E-4	0.0	0.0	14.868563469250152	0.0
37	4.435927463714113E-4	0.0	0.0	15.238741616097094	0.0
38	4.435927463714113E-4	0.0	0.0	15.626663472798892	0.0
39	4.435927463714113E-4	0.0	0.0	16.060275382376947	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.028722E-4	45.0	1
TGCCCTA	20	7.028722E-4	45.0	43
TCACCCG	20	7.028722E-4	45.0	14
TTCGTCA	20	7.028722E-4	45.0	19
ATACTCC	20	7.028722E-4	45.0	27
CTAACGG	25	3.8870217E-5	45.0	2
GTTGTCG	20	7.028722E-4	45.0	37
GTTCGGA	20	7.028722E-4	45.0	45
GCGATAC	20	7.028722E-4	45.0	9
GATTGCG	20	7.028722E-4	45.0	1
TGTAGCG	35	1.2097371E-7	45.0	1
TCATACG	25	3.8870217E-5	45.0	1
TAAACGG	30	2.1625565E-6	44.999996	2
CGAAAGG	60	3.6379788E-12	41.249996	2
TAGACGG	55	6.002665E-11	40.909092	2
CGGGTAT	50	1.0786607E-9	40.5	6
GCGATCA	50	1.0786607E-9	40.5	9
ATGGGCG	95	0.0	40.26316	5
TCAAGCG	95	0.0	40.26316	17
ACGGGAA	165	0.0	39.545456	5
>>END_MODULE