##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936108.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595376
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92263208459864	31.0	31.0	34.0	30.0	34.0
2	32.067656405364005	33.0	31.0	34.0	30.0	34.0
3	31.962675687296766	33.0	31.0	34.0	30.0	34.0
4	35.70621758351025	37.0	35.0	37.0	35.0	37.0
5	31.267415885087743	37.0	35.0	37.0	0.0	37.0
6	33.454077759264734	37.0	35.0	37.0	17.0	37.0
7	29.433688962941066	35.0	32.0	37.0	0.0	37.0
8	32.54698207519282	35.0	33.0	37.0	17.0	37.0
9	36.43752687377388	39.0	35.0	39.0	32.0	39.0
10	36.8028741501169	38.0	35.0	39.0	33.0	39.0
11	36.94618022896455	39.0	37.0	39.0	33.0	39.0
12	36.87313731154766	39.0	35.0	39.0	33.0	39.0
13	36.627284270780144	39.0	35.0	39.0	33.0	39.0
14	37.715606608261	40.0	37.0	41.0	33.0	41.0
15	37.85139306925372	40.0	37.0	41.0	33.0	41.0
16	37.94859718900325	40.0	37.0	41.0	33.0	41.0
17	37.933190118513345	40.0	36.0	41.0	33.0	41.0
18	37.83606829969633	40.0	36.0	41.0	33.0	41.0
19	37.754723065760125	40.0	36.0	41.0	33.0	41.0
20	37.67582502485824	40.0	36.0	41.0	33.0	41.0
21	37.56405531966354	39.0	36.0	41.0	33.0	41.0
22	37.54605828921555	39.0	35.0	41.0	33.0	41.0
23	37.511859732337214	39.0	35.0	41.0	33.0	41.0
24	37.46345838596114	39.0	35.0	41.0	33.0	41.0
25	37.29271922281046	39.0	35.0	41.0	32.0	41.0
26	37.13093910402838	39.0	35.0	41.0	32.0	41.0
27	37.05661296390852	39.0	35.0	41.0	32.0	41.0
28	36.846156042568055	39.0	35.0	41.0	31.0	41.0
29	36.740207868640994	39.0	35.0	41.0	31.0	41.0
30	36.61312683077585	39.0	35.0	41.0	31.0	41.0
31	36.49002815027814	39.0	35.0	40.0	30.0	41.0
32	36.371647496707965	39.0	35.0	40.0	30.0	41.0
33	36.210619843594635	39.0	35.0	40.0	30.0	41.0
34	36.034672207143046	39.0	35.0	40.0	29.0	41.0
35	35.87768065894493	38.0	35.0	40.0	28.0	41.0
36	35.76168001397436	38.0	35.0	40.0	28.0	41.0
37	35.63522379135202	38.0	34.0	40.0	27.0	41.0
38	35.43168350756497	38.0	34.0	40.0	26.0	41.0
39	35.370224866302976	38.0	34.0	40.0	26.0	41.0
40	35.202957458815945	38.0	34.0	40.0	25.0	41.0
41	35.06225981564591	38.0	34.0	40.0	25.0	41.0
42	35.01484943968182	38.0	34.0	40.0	24.0	41.0
43	34.876768630243745	38.0	34.0	40.0	24.0	41.0
44	34.707336204348174	38.0	34.0	40.0	24.0	41.0
45	34.57476619816721	37.0	33.0	40.0	23.0	41.0
46	34.490276732686574	37.0	33.0	40.0	23.0	41.0
47	34.34262046169143	37.0	33.0	40.0	23.0	41.0
48	34.214874297922655	37.0	33.0	40.0	23.0	41.0
49	34.03064785950391	37.0	33.0	40.0	23.0	41.0
50	33.831536709575126	36.0	33.0	40.0	22.0	41.0
51	31.877571484238533	35.0	29.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	25.0
10	33.0
11	37.0
12	29.0
13	32.0
14	34.0
15	55.0
16	92.0
17	193.0
18	394.0
19	727.0
20	1294.0
21	1952.0
22	2841.0
23	3694.0
24	4976.0
25	6461.0
26	7918.0
27	9120.0
28	10230.0
29	11602.0
30	14182.0
31	17644.0
32	22658.0
33	32077.0
34	45547.0
35	51985.0
36	60489.0
37	90430.0
38	106185.0
39	92411.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.903644083738676	20.118378973959313	24.920218483781678	12.05775845852033
2	32.38356937464728	24.50820993792158	25.72441616726237	17.383804520168766
3	33.520330009943294	23.958809223079196	26.314127542930855	16.20673322404665
4	29.047190346940422	25.46575609362823	26.359141114186663	19.127912445244686
5	22.041197495364273	39.480933057429255	22.017011098868615	16.460858348337855
6	26.174551879820484	35.092949665421514	24.594709897610922	14.13778855714708
7	69.78682378866465	19.972084867377927	6.553505683803176	3.6875856601542556
8	85.05163123807476	4.877925882131628	6.771519174437667	3.2989237053559433
9	79.14242428314208	7.031019053505684	9.05696568219075	4.7695909811614845
10	44.17259009432695	25.401091075219696	16.476310768321195	13.950008062132166
11	34.41942570745209	24.405081830641475	22.58068850608691	18.594803955819515
12	31.507316384939937	21.901117948993576	27.685865738625676	18.90569992744081
13	27.4943900997017	22.854968960791165	29.101273816882106	20.549367122625032
14	22.09998387573567	25.59038322001559	30.3611499287845	21.948482975464245
15	22.01751498212894	25.075078605788608	32.41884120287012	20.488565209212332
16	26.361492569401523	23.950075246566875	29.812085136115662	19.87634704791594
17	27.41460858348338	22.930383488753325	26.552296363978396	23.102711563784904
18	27.743308430302864	23.3131668055145	28.447233344978635	20.496291419204
19	28.08863642471312	25.184589234366182	25.56854827873478	21.15822606218591
20	28.781475907661715	25.844004461046467	25.277135793179433	20.097383838112385
21	27.95208406116471	25.513121120098898	27.338858133347664	19.19593668538873
22	26.557167235494884	22.80054956867593	27.870119050818303	22.772164145010883
23	25.58920749240816	24.55255166483029	27.67931525624143	22.178925586520116
24	25.03661551691704	24.102415952272178	28.562958533766896	22.298009997043884
25	25.65622396603155	24.872349574050684	26.71605842358443	22.755368036333344
26	23.91480341834404	25.483559968826423	26.166825669828814	24.434810943000727
27	24.02683346322324	25.01310096476848	27.65798419822096	23.30208137378732
28	21.936053855042864	26.11207035554003	28.40121200720217	23.550663782214937
29	23.746170487221523	26.539699282470234	27.71912203380721	21.995008196501033
30	25.704428797936096	24.68003412969283	27.97929375722233	21.636243315148747
31	27.199618392410844	25.102960146193332	26.609739055655584	21.08768240574024
32	26.643163311923896	25.849211254736502	26.01566069173094	21.491964741608662
33	26.61948079868856	24.957169922872268	26.088891725565023	22.334457552874152
34	24.28952460293999	24.90409421945124	28.751578834215692	22.05480234339308
35	24.058242186450244	26.045221843003414	27.968880169842254	21.927655800704095
36	26.6164574991266	26.599493429362287	25.77010158286528	21.013947488645833
37	24.841444734084007	27.601549273064418	25.420910483459192	22.136095509392383
38	25.255132890811854	27.641859933890515	24.580601166321784	22.52240600897584
39	24.815410765633818	24.80466125608019	25.62212786541614	24.75780011286985
40	26.573963344172423	23.733573405713365	27.687041466233104	22.00542178388111
41	23.23472897799038	24.31774206551826	27.51471339120153	24.932815565289836
42	24.34041681223294	24.522654591384267	27.01334954717691	24.12357904920588
43	23.85400150493134	24.698341888151354	27.416624116524684	24.031032490392626
44	23.693598667060815	24.667437048184677	27.23791352019564	24.401050764558867
45	23.583584155222916	24.43145172126522	26.425821665636505	25.55914245787536
46	24.012388809760555	25.29426782403053	26.343856655290104	24.349486710918814
47	24.016419875843166	25.628174464540056	27.82241810217409	22.53298755744269
48	23.377831823923035	25.906318024240143	27.660335653435812	23.05551449840101
49	23.926224772244765	25.071383461879552	27.759936577893633	23.242455187982046
50	21.989633441724223	26.073607266668457	28.388279003520466	23.548480288086857
51	23.358684260030635	24.7574641906963	26.33915374486039	25.544697804412674
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	396.0
1	822.0
2	1248.0
3	2253.0
4	3258.0
5	2527.0
6	1796.0
7	1518.5
8	1241.0
9	1244.5
10	1248.0
11	1246.0
12	1244.0
13	1259.5
14	1275.0
15	1316.5
16	1358.0
17	1461.0
18	1564.0
19	1610.5
20	1657.0
21	1706.5
22	1756.0
23	2052.0
24	2348.0
25	2790.5
26	4206.0
27	5179.0
28	6260.0
29	7341.0
30	8068.0
31	8795.0
32	10445.5
33	12096.0
34	13781.5
35	15467.0
36	15807.5
37	16148.0
38	17452.5
39	18757.0
40	20734.5
41	22712.0
42	25245.5
43	27779.0
44	29491.5
45	31204.0
46	34025.0
47	36846.0
48	39981.5
49	43117.0
50	44562.5
51	46008.0
52	45439.0
53	44870.0
54	42905.5
55	40941.0
56	39315.5
57	37690.0
58	37008.5
59	36327.0
60	34856.0
61	33385.0
62	30807.5
63	28230.0
64	24689.5
65	21149.0
66	17411.0
67	13673.0
68	11287.5
69	8902.0
70	7443.5
71	5985.0
72	4938.0
73	3891.0
74	3172.0
75	1868.0
76	1283.0
77	988.0
78	693.0
79	530.5
80	368.0
81	289.5
82	211.0
83	164.0
84	117.0
85	86.5
86	56.0
87	44.5
88	33.0
89	27.5
90	22.0
91	16.0
92	10.0
93	7.5
94	5.0
95	6.5
96	8.0
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	595376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.67147840142747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.07887008162943	27.90660554282255
2	8.150067808002337	6.140502067986588
3	3.774545133386219	4.265780864027165
4	2.514510218372552	3.7890126952636107
5	1.8074770412892238	3.4045166161001514
6	1.290344780872855	2.9165517325827857
7	0.9887335351813653	2.6072937810746284
8	0.7932605366336377	2.390663773799908
9	0.6306088260473395	2.1380370093172694
>10	5.903522364820311	41.62671898710729
>50	0.04537311584481623	1.131025516321304
>100	0.022232826761546694	1.4578383901875063
>500	0.0	0.0
>1k	4.537311583989121E-4	0.2254530234092298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1319	0.22154067345677353	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04669318212356561	0.0
2	0.0	0.0	0.0	0.2275872725806885	0.0
3	0.0	0.0	0.0	0.3250047029104297	0.0
4	0.0	0.0	0.0	0.4477842573432587	0.0
5	0.0	0.0	0.0	0.8115879712988094	0.0
6	0.0	0.0	0.0	1.1197965655316977	0.0
7	0.0	0.0	0.0	1.383663432855876	0.0
8	0.0	0.0	0.0	1.8474039934427993	0.0
9	0.0	0.0	0.0	2.0511407917013784	0.0
10	0.0	0.0	0.0	2.535372604874903	0.0
11	0.0	0.0	0.0	3.1613635752868774	0.0
12	0.0	0.0	0.0	3.6953118701459244	0.0
13	0.0	0.0	0.0	3.958003009862675	0.0
14	0.0	0.0	0.0	4.096402945365618	0.0
15	0.0	0.0	0.0	4.264531993227809	0.0
16	0.0	0.0	0.0	4.607844454596759	0.0
17	0.0	0.0	0.0	4.994322915266991	0.0
18	0.0	0.0	0.0	5.431525624143399	0.0
19	0.0	0.0	0.0	5.687498320389132	0.0
20	0.0	0.0	0.0	5.964466152481793	0.0
21	0.0	0.0	0.0	6.309122302544947	0.0
22	0.0	0.0	0.0	6.6465561258767565	0.0
23	0.0	0.0	0.0	6.999946252452232	0.0
24	0.0	0.0	0.0	7.272211174115181	0.0
25	0.0	0.0	0.0	7.53456639165838	0.0
26	0.0	0.0	0.0	7.775254629007551	0.0
27	0.0	0.0	0.0	8.041137029373035	0.0
28	1.6796108677541587E-4	0.0	0.0	8.301140791701378	0.0
29	1.6796108677541587E-4	0.0	0.0	8.578276584880815	0.0
30	1.6796108677541587E-4	0.0	0.0	8.971641450108839	0.0
31	1.6796108677541587E-4	0.0	0.0	9.285896643465643	0.0
32	3.3592217355083175E-4	0.0	0.0	9.556481954260837	0.0
33	3.3592217355083175E-4	0.0	0.0	9.845375023514553	0.0
34	3.3592217355083175E-4	0.0	0.0	10.168868076644003	0.0
35	3.3592217355083175E-4	0.0	0.0	10.529312568864045	0.0
36	3.3592217355083175E-4	0.0	0.0	10.844575528741501	0.0
37	3.3592217355083175E-4	0.0	0.0	11.17176372578001	0.0
38	3.3592217355083175E-4	0.0	0.0	11.52834511300422	0.0
39	3.3592217355083175E-4	0.0	0.0	12.122759399102415	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	35	1.2106102E-7	45.000004	1
CCCGTAT	20	7.030543E-4	45.000004	17
TCGACAA	20	7.030543E-4	45.000004	19
TCGGTAA	20	7.030543E-4	45.000004	27
GTCGGTA	20	7.030543E-4	45.000004	26
TACGGGT	50	2.1827873E-11	45.0	4
CGATATG	25	3.8885333E-5	45.0	10
GCGATAT	65	0.0	41.538464	9
CGGGTAT	55	6.002665E-11	40.90909	6
ACTACGG	45	1.9255822E-8	40.0	2
CACGACG	40	3.4544792E-7	39.375004	26
TGCGATA	40	3.4544792E-7	39.375004	1
CGGTCTA	40	3.4544792E-7	39.375004	31
GACCGAT	300	0.0	39.000004	9
GTATGCG	35	6.2432155E-6	38.57143	1
CGAATAT	105	0.0	38.571426	14
CGTTATT	100	0.0	38.25	1
TTCGTAC	30	1.1392613E-4	37.499996	33
CGGCTAT	30	1.1392613E-4	37.499996	41
TTTGACG	30	1.1392613E-4	37.499996	1
>>END_MODULE