##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936106.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	208898
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01737690164578	33.0	31.0	34.0	30.0	34.0
2	32.16399869792913	33.0	31.0	34.0	30.0	34.0
3	32.11078133826078	33.0	31.0	34.0	30.0	34.0
4	35.822248178536896	37.0	35.0	37.0	35.0	37.0
5	31.36822755603213	37.0	35.0	37.0	0.0	37.0
6	33.5293540388132	37.0	35.0	37.0	17.0	37.0
7	29.408472077281736	35.0	32.0	37.0	0.0	37.0
8	32.53947381018487	35.0	33.0	37.0	17.0	37.0
9	36.44968836465644	39.0	35.0	39.0	32.0	39.0
10	36.913086769619625	38.0	37.0	39.0	34.0	39.0
11	37.02383459870367	39.0	37.0	39.0	34.0	39.0
12	36.94094725655583	39.0	35.0	39.0	33.0	39.0
13	36.63124108416548	39.0	35.0	39.0	33.0	39.0
14	37.837906538119086	40.0	36.0	41.0	33.0	41.0
15	38.00912407012034	40.0	37.0	41.0	33.0	41.0
16	38.10692299591188	40.0	36.0	41.0	34.0	41.0
17	38.10008233683424	40.0	36.0	41.0	34.0	41.0
18	38.04689848634262	40.0	36.0	41.0	34.0	41.0
19	37.96798916217484	40.0	36.0	41.0	34.0	41.0
20	37.87498683568057	40.0	36.0	41.0	34.0	41.0
21	37.7378720715373	40.0	36.0	41.0	33.0	41.0
22	37.73063408936419	40.0	36.0	41.0	33.0	41.0
23	37.712165745962146	40.0	35.0	41.0	33.0	41.0
24	37.68890080326284	40.0	35.0	41.0	33.0	41.0
25	37.56298289117177	39.0	35.0	41.0	33.0	41.0
26	37.355072810654	39.0	35.0	41.0	33.0	41.0
27	37.32524485634137	39.0	35.0	41.0	33.0	41.0
28	37.210811017817306	39.0	35.0	41.0	32.0	41.0
29	37.217421899683096	39.0	35.0	41.0	32.0	41.0
30	37.134223400894214	39.0	35.0	41.0	32.0	41.0
31	37.03656808586008	39.0	35.0	41.0	32.0	41.0
32	36.942704094821394	39.0	35.0	41.0	32.0	41.0
33	36.80185545098565	39.0	35.0	41.0	31.0	41.0
34	36.6673831247786	39.0	35.0	41.0	31.0	41.0
35	36.61058028320041	39.0	35.0	41.0	31.0	41.0
36	36.46335053471072	39.0	35.0	41.0	30.0	41.0
37	36.32059665482676	39.0	35.0	40.0	30.0	41.0
38	36.21673735507281	39.0	35.0	40.0	30.0	41.0
39	36.17984853852119	39.0	35.0	40.0	30.0	41.0
40	35.99988511139408	38.0	35.0	40.0	29.0	41.0
41	35.88071211787571	38.0	35.0	40.0	29.0	41.0
42	35.817714865628204	38.0	35.0	40.0	29.0	41.0
43	35.63909659259543	38.0	35.0	40.0	28.0	41.0
44	35.418955662572166	38.0	34.0	40.0	27.0	41.0
45	35.3244693582514	38.0	34.0	40.0	27.0	41.0
46	35.1803607502226	38.0	34.0	40.0	26.0	41.0
47	35.029425844191906	38.0	34.0	40.0	26.0	41.0
48	34.91346015758887	38.0	34.0	40.0	25.0	41.0
49	34.770931267891505	37.0	34.0	40.0	24.0	41.0
50	34.57091020498042	37.0	34.0	40.0	24.0	41.0
51	32.609359591762484	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	7.0
10	17.0
11	10.0
12	10.0
13	1.0
14	5.0
15	17.0
16	26.0
17	49.0
18	83.0
19	163.0
20	255.0
21	416.0
22	679.0
23	933.0
24	1242.0
25	1617.0
26	2066.0
27	2444.0
28	2762.0
29	3493.0
30	4316.0
31	5583.0
32	7322.0
33	10958.0
34	16863.0
35	20100.0
36	19737.0
37	31128.0
38	39377.0
39	37206.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07269576539747	20.88770596176124	24.791525050503115	11.248073222338174
2	31.940468554031153	24.72785761472106	26.341563825407615	16.99011000584017
3	32.82798303478253	24.478453599364283	25.818341965935527	16.875221399917663
4	29.946672538750967	26.390870185449355	25.56415092533198	18.09830635046769
5	22.42625587607349	41.06166645922891	19.85418721098335	16.657890453714252
6	26.769523882468953	35.98598359007745	23.477486620264436	13.767005907189153
7	68.7110455820544	20.558837327308062	6.671198383900277	4.058918706737259
8	82.94717996342713	6.120211777996917	7.2825015079129525	3.650106750663003
9	76.80446916677039	8.629570412354354	9.240394833842354	5.325565587032906
10	43.82760964681328	28.176430602494996	15.376403795153614	12.61955595553811
11	35.91561431894992	26.786278470832652	21.326676176890157	15.971431033327269
12	33.17695717527214	24.267824488506353	25.426284598224967	17.128933737996537
13	25.446390104261408	28.49620388897931	26.6766555926816	19.38075041407768
14	18.94991814186828	33.827035203783666	26.733142490593497	20.48990416375456
15	19.068636367988205	27.286043906595562	35.50967457802373	18.135645147392506
16	21.516721079186972	25.067736407241814	34.37323478444026	19.042307729130965
17	20.59282520656014	24.262080058210227	28.789648536606382	26.35544619862325
18	23.06628115156679	27.661825388467097	27.464121245775452	21.807772214190656
19	27.711131748508844	27.314287355551514	25.095501153673084	19.879079742266562
20	29.302817643060248	25.540215799098124	26.986854828672367	18.17011172916926
21	24.97199590230639	26.736493408266238	27.589062604716176	20.702448084711197
22	22.213233252592175	27.423431531177894	26.04189604495974	24.32143917127019
23	22.582791601642906	28.89544179456002	26.733142490593497	21.78862411320357
24	22.536836159273903	25.827437313904394	29.343507357657806	22.2922191691639
25	21.901119206502695	27.057224099799903	28.787733726507675	22.25392296718973
26	20.889620771859953	31.263583184137712	27.251098622294133	20.595697421708202
27	22.363067142816114	30.645578224779555	27.40380472766613	19.587549904738196
28	19.89056860285881	30.152993326886808	30.591006136966364	19.365431933288015
29	22.196957366753153	27.233386628881078	30.397131614472134	20.17252438989363
30	22.261103505059886	27.289394824268303	28.65417572212276	21.795325948549053
31	24.44111480243947	29.02229796359946	25.08640580570422	21.450181428256855
32	25.941368514777547	28.777202270964775	24.93178489023351	20.349644324024165
33	23.63593715593256	29.7159379218566	25.814033643213435	20.834091278997406
34	22.689542264645905	28.65848404484485	27.58188206684602	21.070091623663224
35	23.054313588449865	26.374115597085655	27.544543269921206	23.02702754454327
36	24.03756857413666	28.483278920813028	26.237206675028002	21.241945830022306
37	23.718273990177025	29.755191528880125	27.829849974628768	18.69668450631409
38	22.644065524801576	28.951928692471924	25.25060077166847	23.15340501105803
39	24.373617746459995	26.259226991163153	26.60102059378261	22.766134668594244
40	24.2166033183659	25.719250543327366	28.905015845053565	21.159130293253167
41	20.282625970569367	27.10557305479229	29.377973939434554	23.233827035203785
42	23.674712060431407	26.929410525711113	27.680973489454185	21.7149039244033
43	23.865714367777578	26.342999932981648	27.211366312745934	22.579919386494847
44	22.86426868615305	28.351157024002145	27.064883340194733	21.71969094965007
45	23.336748078009364	27.667569818763226	24.5785024270218	24.417179676205613
46	22.100259456768377	30.443565759365814	25.320012637746654	22.136162146119158
47	23.25776216143764	27.044299131633622	28.01462914915413	21.683309557774606
48	22.942775900200097	24.683816982450764	28.72741720839836	23.645989908950778
49	21.117961876130934	26.52825781003169	29.2410650173769	23.112715296460472
50	20.30895460942661	27.25875786268897	28.408122624438718	24.0241649034457
51	20.970042796005707	27.72070579900238	26.230983542207202	25.07826786278471
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	251.0
1	389.5
2	528.0
3	589.0
4	650.0
5	549.5
6	449.0
7	416.0
8	383.0
9	391.5
10	400.0
11	411.0
12	422.0
13	443.0
14	464.0
15	507.0
16	550.0
17	555.5
18	561.0
19	588.0
20	615.0
21	740.0
22	865.0
23	953.5
24	1042.0
25	1397.5
26	1918.5
27	2084.0
28	2591.5
29	3099.0
30	3758.0
31	4417.0
32	5107.0
33	5797.0
34	6008.5
35	6220.0
36	6532.5
37	6845.0
38	7637.5
39	8430.0
40	10003.5
41	11577.0
42	12771.0
43	13965.0
44	15646.5
45	17328.0
46	17191.5
47	17055.0
48	17230.0
49	17405.0
50	16454.5
51	15504.0
52	14153.5
53	12803.0
54	11373.0
55	9943.0
56	9560.5
57	9178.0
58	9175.0
59	9172.0
60	9157.0
61	9142.0
62	8183.0
63	7224.0
64	6175.5
65	5127.0
66	4116.0
67	3105.0
68	2486.0
69	1867.0
70	1485.0
71	1103.0
72	883.0
73	663.0
74	567.5
75	343.0
76	214.0
77	162.5
78	111.0
79	81.0
80	51.0
81	41.0
82	31.0
83	22.0
84	13.0
85	10.5
86	8.0
87	7.0
88	6.0
89	5.0
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	208898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.10374441114803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13206046141607	26.04237474748442
2	8.525590029169981	6.156114467347701
3	4.175285070273137	4.522302750624707
4	2.7844073190135243	4.021101207287767
5	1.9968178202068416	3.604630010818677
6	1.431980906921241	3.101992359907706
7	1.0766374966852292	2.7209451502647224
8	0.8843808008485813	2.5543566716770867
9	0.6616282153274994	2.149853038324924
>10	6.2556351100503855	42.79648440865877
>50	0.057014054627419784	1.2666468802956468
>100	0.01856271546009016	1.0631983073078728
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	313	0.14983389022393703	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	251	0.1201543336939559	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09191088473800611	0.0
2	0.0	0.0	0.0	0.4226943292898927	0.0
3	0.0	0.0	0.0	0.5897615104022058	0.0
4	0.0	0.0	0.0	0.7869869505691773	0.0
5	0.0	0.0	0.0	1.4289270361611888	0.0
6	0.0	0.0	0.0	1.8004001953106301	0.0
7	0.0	0.0	0.0	2.1847983226263534	0.0
8	0.0	0.0	0.0	2.7961014466390295	0.0
9	0.0	0.0	0.0	3.051728594816609	0.0
10	0.0	0.0	0.0	3.7185612116918305	0.0
11	0.0	0.0	0.0	4.479219523403766	0.0
12	0.0	0.0	0.0	5.163764133692041	0.0
13	0.0	0.0	0.0	5.421784794493006	0.0
14	0.0	0.0	0.0	5.540503020612931	0.0
15	0.0	0.0	0.0	5.7927792511177705	0.0
16	0.0	0.0	0.0	6.236057788968779	0.0
17	0.0	0.0	0.0	6.747790787848615	0.0
18	0.0	0.0	0.0	7.417495619871899	0.0
19	0.0	0.0	0.0	7.769820678034256	0.0
20	0.0	0.0	0.0	8.090072667043247	0.0
21	0.0	0.0	0.0	8.48260873727848	0.0
22	0.0	0.0	0.0	8.937854838246418	0.0
23	0.0	0.0	0.0	9.355762142289539	0.0
24	0.0	0.0	0.0	9.710002010550603	0.0
25	0.0	0.0	0.0	9.964671753678829	0.0
26	0.0	0.0	0.0	10.228915547300597	0.0
27	0.0	0.0	0.0	10.4673094045898	0.0
28	0.0	0.0	0.0	10.744956868902527	0.0
29	0.0	0.0	0.0	11.022125630690576	0.0
30	0.0	0.0	0.0	11.33519708182941	0.0
31	0.0	0.0	0.0	11.651140748116305	0.0
32	0.0	0.0	0.0	11.948893718465472	0.0
33	0.0	0.0	0.0	12.255742036783502	0.0
34	0.0	0.0	0.0	12.535304311194936	0.0
35	0.0	0.0	0.0	12.823004528525884	0.0
36	0.0	0.0	0.0	13.119800093825695	0.0
37	0.0	0.0	0.0	13.405106798533255	0.0
38	0.0	0.0	0.0	13.654989516414709	0.0
39	0.0	0.0	0.0	13.942211031220978	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	20	7.0200197E-4	45.000004	43
CATATGC	20	7.0200197E-4	45.000004	33
CCCTCGT	40	6.7684596E-9	45.000004	14
TAGGTCC	20	7.0200197E-4	45.000004	6
GGATCGT	20	7.0200197E-4	45.000004	8
CATCTCG	20	7.0200197E-4	45.000004	42
CCTCGTG	40	6.7684596E-9	45.000004	15
GTTAACG	20	7.0200197E-4	45.000004	1
CGTAGGT	20	7.0200197E-4	45.000004	39
CTACGAA	35	1.2055534E-7	45.000004	11
TACGAAT	35	1.2055534E-7	45.000004	12
GCCCTCG	40	6.7684596E-9	45.000004	13
CGAATAT	35	1.2055534E-7	45.000004	14
GCGATAA	35	1.2055534E-7	45.000004	9
GTATCCC	20	7.0200197E-4	45.000004	39
GCTACGA	35	1.2055534E-7	45.000004	10
ATTATAC	35	1.2055534E-7	45.000004	42
GCATCAA	20	7.0200197E-4	45.000004	44
GCATATG	20	7.0200197E-4	45.000004	32
GCGAGAC	25	3.8798134E-5	45.0	21
>>END_MODULE