FastQCFastQC Report
Sat 14 Jan 2017
SRR2936101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114403
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103500.9287483971238412No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29380.2636389169806614No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC27770.2491917196920683No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.18117323804763627No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG17210.1544324629420416No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC15840.14213888512504005No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.12203843672351923No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG253.8907296E-545.01
TATAGCG453.8562575E-1045.01
CGTTATT7150.042.482521
CGTTTTT38600.041.152851
CCCGTTA451.9281288E-840.035
TATACGG850.039.7058832
CGGTTTT9850.039.517771
CGTTTTA4850.039.432991
TTACGGG3050.038.3606573
GTTACGG1250.037.8000032
CGCGTAG301.13989954E-437.5000042
TACGGGA4250.037.0588264
ATGGGCG3100.037.016135
TTTACGG1100.036.8181842
ACGGGAT3200.036.5625045
TTATGCG504.8787115E-836.01
TTACCGG250.002107081236.02
AATCGTC250.002107081236.030
TACGGTT458.699608E-735.033
GCGATAC458.699608E-735.09
Produced by FastQC (version 0.11.5)