##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936101.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1114403
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83922064100689	31.0	31.0	34.0	30.0	34.0
2	31.94869540013801	33.0	31.0	34.0	30.0	34.0
3	31.62785634999188	33.0	31.0	34.0	30.0	34.0
4	35.50904475310996	37.0	35.0	37.0	33.0	37.0
5	31.090293188370815	37.0	35.0	37.0	0.0	37.0
6	33.33456478491174	37.0	35.0	37.0	17.0	37.0
7	29.53540056873501	35.0	32.0	37.0	0.0	37.0
8	32.55986748061518	35.0	33.0	37.0	17.0	37.0
9	36.44558386867229	39.0	35.0	39.0	32.0	39.0
10	36.84440547988475	38.0	37.0	39.0	33.0	39.0
11	36.96525852855744	39.0	37.0	39.0	33.0	39.0
12	36.95022446996284	39.0	37.0	39.0	33.0	39.0
13	36.77220000305096	39.0	35.0	39.0	33.0	39.0
14	37.950387786106106	40.0	37.0	41.0	33.0	41.0
15	38.04428828709183	40.0	37.0	41.0	33.0	41.0
16	38.11632147436789	40.0	37.0	41.0	33.0	41.0
17	38.08457443133229	40.0	37.0	41.0	33.0	41.0
18	37.943335579678084	40.0	37.0	41.0	33.0	41.0
19	37.798848351987566	40.0	37.0	41.0	33.0	41.0
20	37.65912421269505	39.0	36.0	41.0	33.0	41.0
21	37.568769107764425	39.0	35.0	41.0	33.0	41.0
22	37.57378704113324	39.0	35.0	41.0	33.0	41.0
23	37.52828734308863	39.0	35.0	41.0	33.0	41.0
24	37.45202678025813	39.0	35.0	41.0	33.0	41.0
25	37.30668079680331	39.0	35.0	41.0	32.0	41.0
26	37.15700334618625	39.0	35.0	41.0	32.0	41.0
27	37.112296000638906	39.0	35.0	41.0	32.0	41.0
28	36.96334180722773	39.0	35.0	41.0	32.0	41.0
29	36.88456509898125	39.0	35.0	41.0	31.0	41.0
30	36.71575004733476	39.0	35.0	41.0	31.0	41.0
31	36.539248368857585	39.0	35.0	40.0	30.0	41.0
32	36.367717961994	39.0	35.0	40.0	30.0	41.0
33	36.11205012908257	39.0	35.0	40.0	30.0	41.0
34	35.85851437944801	38.0	35.0	40.0	28.0	41.0
35	35.63889544446668	38.0	35.0	40.0	27.0	41.0
36	35.4377563592345	38.0	34.0	40.0	26.0	41.0
37	35.31100508523398	38.0	34.0	40.0	25.0	41.0
38	35.1185738013986	38.0	34.0	40.0	24.0	41.0
39	35.048759739519724	38.0	34.0	40.0	24.0	41.0
40	34.85100273419939	38.0	34.0	40.0	23.0	41.0
41	34.73806872379202	38.0	34.0	40.0	23.0	41.0
42	34.654800821605825	38.0	34.0	40.0	23.0	41.0
43	34.54047144524916	38.0	33.0	40.0	23.0	41.0
44	34.346992963945716	38.0	33.0	40.0	22.0	41.0
45	34.243280931583996	37.0	33.0	40.0	22.0	41.0
46	34.10381522662807	37.0	33.0	40.0	21.0	41.0
47	33.918331160271464	37.0	33.0	40.0	20.0	41.0
48	33.797590279279575	37.0	33.0	40.0	20.0	41.0
49	33.653932195085616	37.0	33.0	40.0	20.0	41.0
50	33.49408427651397	36.0	33.0	40.0	19.0	41.0
51	31.541720544542684	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	20.0
9	41.0
10	71.0
11	45.0
12	37.0
13	30.0
14	77.0
15	113.0
16	215.0
17	386.0
18	666.0
19	1130.0
20	2033.0
21	3096.0
22	4809.0
23	7334.0
24	10678.0
25	14993.0
26	18609.0
27	20819.0
28	21662.0
29	24017.0
30	27228.0
31	33481.0
32	42462.0
33	58498.0
34	80933.0
35	94058.0
36	108715.0
37	163021.0
38	195432.0
39	179673.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45277516302451	19.498332290921685	22.48001844934014	15.568874096713667
2	35.082191989791845	24.46000235103459	23.87071822312036	16.587087436053206
3	32.4806196681093	24.162802863954962	27.93800806351024	15.418569404425508
4	27.560496516969174	24.859409028870168	29.35930718061599	18.22078727354467
5	21.625659658130854	37.95368461858053	23.899702351842198	16.520953371446414
6	25.1802983301373	33.396446348403586	27.721479572470642	13.70177574898847
7	66.33973526632644	19.295533124013488	10.531019747793213	3.8337118618668473
8	80.22169717777142	5.215169018748155	11.166158023623412	3.396975779857018
9	74.60550626658399	6.700897251712353	12.853518879615363	5.840077602088293
10	40.80660227942674	25.070732939520084	20.02749454192065	14.095170239132523
11	30.564436743260742	25.41217135991199	26.714213798778356	17.30917809804891
12	27.5928905431877	22.938829130933783	31.161886678338085	18.306393647540432
13	24.598821072807592	22.712519618127374	33.67857049918207	19.010088809882962
14	20.69870594390001	25.488265914574885	33.327620259457305	20.485407882067797
15	19.973923257564813	24.98512656552432	35.39473601560657	19.646214161304304
16	24.60510246293307	24.827284205085594	31.457650419103324	19.109962912878018
17	24.956591107525732	24.366140435731058	30.010328400049175	20.666940056694035
18	25.102588560870707	24.526944022943226	31.077985253090667	19.292482163095396
19	24.58428414137435	26.26644041697662	29.007369865300074	20.14190557634895
20	25.174196408301125	26.70021527221301	29.594949044465963	18.530639275019897
21	25.008995848001124	25.849445846789713	30.639275019898548	18.50228328531061
22	23.899971554276146	23.143512714879627	31.589110941015054	21.367404789829173
23	22.660294345941278	25.464486366242735	31.64304116194949	20.232178125866497
24	23.195199582197823	24.557004961400857	31.06587114356297	21.181924312838355
25	23.462786801543068	26.558166121232624	29.277559374840163	20.701487702384146
26	22.508374439049426	25.71933133704773	29.37348517547063	22.398809048432213
27	22.29794786984601	25.042825620534042	30.913592300092517	21.745634209527434
28	20.56141270258605	25.99903266592068	31.081574618876655	22.357980012616622
29	22.215033520189735	25.626635965624644	30.81712809459415	21.341202419591475
30	22.919715758123406	24.404456915496457	31.076998177499522	21.59882914888061
31	23.812749965676687	25.682271135307428	29.33848885905727	21.166490039958617
32	24.080157716732636	25.19905276636908	30.223177791158136	20.49761172574015
33	23.89602325191156	25.156518781805147	29.056723644857378	21.89073432142591
34	22.026591816425476	25.579884476262176	30.993007018107456	21.400516689204892
35	21.877722870451713	26.49409594195278	29.97380660317677	21.654374584418743
36	22.926356084827482	27.892961522896115	27.719774623722298	21.460907768554105
37	21.729482063490497	27.774243249524634	28.40489481812235	22.09137986886252
38	22.65697418258924	27.519667481153586	27.895294610657007	21.928063725600165
39	23.063021187128893	25.573513351992055	27.99229722102328	23.37116823985578
40	24.344693975159796	25.295337503578146	29.354371802660257	21.005596718601797
41	21.18542394447969	26.090202556884716	28.709452505063247	24.014920993572346
42	21.34003587571103	26.281156816699163	29.814079825700396	22.564727481889406
43	22.537448301915912	25.208833788135887	29.14681672608563	23.106901183862572
44	22.499131822150513	24.960988080613568	29.061120617945214	23.478759479290705
45	22.280898382362572	24.97211511455012	28.345131877785683	24.40185462530162
46	22.47750589328995	25.867123473285698	28.44814667584348	23.207223957580876
47	21.516901874815485	25.504238592322526	30.44338538212837	22.535474150733624
48	21.456600529610924	26.050899001528173	29.575656203366286	22.916844265494618
49	22.007119507036503	25.35303655858787	29.81784865977568	22.821995274599942
50	20.697180463440965	25.685142627936212	30.204154152492414	23.413522756130412
51	21.237021077653235	24.687388673576795	29.26042015321208	24.81517009555789
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	595.0
1	1300.0
2	2005.0
3	11572.5
4	21140.0
5	15508.5
6	9877.0
7	7808.5
8	5740.0
9	5439.5
10	5139.0
11	5064.5
12	4990.0
13	4791.0
14	4592.0
15	4396.0
16	4200.0
17	4200.0
18	4200.0
19	4300.0
20	4400.0
21	4707.0
22	5014.0
23	5648.5
24	6283.0
25	7246.0
26	9679.5
27	11150.0
28	12992.0
29	14834.0
30	17552.0
31	20270.0
32	23465.0
33	26660.0
34	30113.0
35	33566.0
36	35568.0
37	37570.0
38	40165.0
39	42760.0
40	45376.5
41	47993.0
42	51583.0
43	55173.0
44	61262.5
45	67352.0
46	75970.0
47	84588.0
48	83439.0
49	82290.0
50	81410.0
51	80530.0
52	75680.0
53	70830.0
54	66480.0
55	62130.0
56	60297.5
57	58465.0
58	56871.5
59	55278.0
60	51777.0
61	48276.0
62	44081.5
63	39887.0
64	34612.0
65	29337.0
66	24973.0
67	20609.0
68	17380.5
69	14152.0
70	11517.5
71	8883.0
72	7582.5
73	6282.0
74	5189.0
75	3261.5
76	2427.0
77	1792.0
78	1157.0
79	902.0
80	647.0
81	513.0
82	379.0
83	290.5
84	202.0
85	157.0
86	112.0
87	88.0
88	64.0
89	48.0
90	32.0
91	25.0
92	18.0
93	15.0
94	12.0
95	9.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1114403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.286518213715965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.2777066744109	25.504286130122196
2	8.746806291727472	6.172886790497737
3	4.154432327518449	4.397863559778903
4	2.758922328244306	3.894110519432814
5	1.903964085264466	3.3592131686472815
6	1.4184940697880182	3.003223009777379
7	1.0732997788433858	2.6511108536454113
8	0.845180367220322	2.385877793743603
9	0.6579369887069422	2.0894674981936454
>10	6.073602825576558	41.14418683679009
>50	0.06278527718299882	1.4256364302464943
>100	0.02299365108556193	1.4121413005284795
>500	0.0020668450296363675	0.48609021227997457
>1k	0.0015501337722272754	1.1303511894334968
>5k	0.0	0.0
>10k+	2.5835562870454593E-4	0.9435547068825464
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10350	0.9287483971238412	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2938	0.2636389169806614	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	2777	0.2491917196920683	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2019	0.18117323804763627	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	1721	0.1544324629420416	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	1584	0.14213888512504005	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1360	0.12203843672351923	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.973414464964649E-5	0.0	0.0	0.045315743048071476	0.0
2	8.973414464964649E-5	0.0	0.0	0.20602959611558835	0.0
3	8.973414464964649E-5	0.0	0.0	0.3134413672612152	0.0
4	8.973414464964649E-5	0.0	0.0	0.521893785282344	0.0
5	8.973414464964649E-5	0.0	0.0	1.3014142998538232	0.0
6	8.973414464964649E-5	0.0	0.0	1.851753808990105	0.0
7	8.973414464964649E-5	0.0	0.0	2.2604031037245953	0.0
8	8.973414464964649E-5	0.0	0.0	2.8725694385244833	0.0
9	8.973414464964649E-5	0.0	0.0	3.221455792922309	0.0
10	8.973414464964649E-5	0.0	0.0	3.997207473418503	0.0
11	8.973414464964649E-5	0.0	0.0	4.537586492498674	0.0
12	8.973414464964649E-5	0.0	0.0	5.146791600525124	0.0
13	8.973414464964649E-5	0.0	0.0	5.377049415696117	0.0
14	8.973414464964649E-5	0.0	0.0	5.530764005480962	0.0
15	1.7946828929929297E-4	0.0	0.0	5.689413973221536	0.0
16	1.7946828929929297E-4	0.0	0.0	5.935106061272268	0.0
17	1.7946828929929297E-4	0.0	0.0	6.2201017046795455	0.0
18	1.7946828929929297E-4	0.0	0.0	6.536773501148148	0.0
19	1.7946828929929297E-4	0.0	0.0	6.733650214509473	0.0
20	1.7946828929929297E-4	0.0	0.0	6.949730034825822	0.0
21	1.7946828929929297E-4	0.0	0.0	7.206100486089862	0.0
22	1.7946828929929297E-4	0.0	0.0	7.4724314274100125	0.0
23	1.7946828929929297E-4	0.0	0.0	7.748094719773726	0.0
24	1.7946828929929297E-4	0.0	0.0	7.969109918045806	0.0
25	1.7946828929929297E-4	0.0	0.0	8.182049043299417	0.0
26	1.7946828929929297E-4	0.0	0.0	8.390770663754495	0.0
27	1.7946828929929297E-4	0.0	0.0	8.60801702795129	0.0
28	1.7946828929929297E-4	0.0	0.0	8.821135621494198	0.0
29	1.7946828929929297E-4	0.0	0.0	9.061084724287355	0.0
30	1.7946828929929297E-4	0.0	0.0	9.41248363473537	0.0
31	1.7946828929929297E-4	0.0	0.0	9.677378829741127	0.0
32	1.7946828929929297E-4	0.0	0.0	9.965874104789739	0.0
33	1.7946828929929297E-4	0.0	0.0	10.2170399756641	0.0
34	1.7946828929929297E-4	0.0	0.0	10.485075865732593	0.0
35	1.7946828929929297E-4	0.0	0.0	10.791248767277187	0.0
36	1.7946828929929297E-4	0.0	0.0	11.067899135232048	0.0
37	1.7946828929929297E-4	0.0	0.0	11.350471956733784	0.0
38	1.7946828929929297E-4	0.0	0.0	11.68410350654117	0.0
39	1.7946828929929297E-4	0.0	0.0	12.200972179723134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	25	3.8907296E-5	45.0	1
TATAGCG	45	3.8562575E-10	45.0	1
CGTTATT	715	0.0	42.48252	1
CGTTTTT	3860	0.0	41.15285	1
CCCGTTA	45	1.9281288E-8	40.0	35
TATACGG	85	0.0	39.705883	2
CGGTTTT	985	0.0	39.51777	1
CGTTTTA	485	0.0	39.43299	1
TTACGGG	305	0.0	38.360657	3
GTTACGG	125	0.0	37.800003	2
CGCGTAG	30	1.13989954E-4	37.500004	2
TACGGGA	425	0.0	37.058826	4
ATGGGCG	310	0.0	37.01613	5
TTTACGG	110	0.0	36.818184	2
ACGGGAT	320	0.0	36.562504	5
TTATGCG	50	4.8787115E-8	36.0	1
TTACCGG	25	0.0021070812	36.0	2
AATCGTC	25	0.0021070812	36.0	30
TACGGTT	45	8.699608E-7	35.0	33
GCGATAC	45	8.699608E-7	35.0	9
>>END_MODULE