##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936099.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	311409
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.864522219974376	31.0	31.0	34.0	30.0	34.0
2	31.99338169417069	33.0	31.0	34.0	30.0	34.0
3	31.92277037593647	33.0	31.0	34.0	30.0	34.0
4	35.667713521446075	37.0	35.0	37.0	33.0	37.0
5	31.29231974669968	37.0	35.0	37.0	0.0	37.0
6	33.436287968555824	37.0	35.0	37.0	17.0	37.0
7	29.384092945290597	35.0	32.0	37.0	0.0	37.0
8	32.47402611999011	35.0	33.0	37.0	17.0	37.0
9	36.34889807295229	39.0	35.0	39.0	32.0	39.0
10	36.757524670128355	38.0	35.0	39.0	33.0	39.0
11	36.896255406876485	39.0	37.0	39.0	33.0	39.0
12	36.835162117986314	39.0	35.0	39.0	33.0	39.0
13	36.57325896168704	39.0	35.0	39.0	32.0	39.0
14	37.81161430787164	40.0	36.0	41.0	33.0	41.0
15	37.914177175354595	40.0	36.0	41.0	33.0	41.0
16	37.993680336791805	40.0	36.0	41.0	33.0	41.0
17	37.94615441429117	40.0	36.0	41.0	33.0	41.0
18	37.86600579944703	40.0	36.0	41.0	33.0	41.0
19	37.74518077512211	40.0	36.0	41.0	33.0	41.0
20	37.69143473695365	39.0	36.0	41.0	33.0	41.0
21	37.58154709722584	39.0	36.0	41.0	33.0	41.0
22	37.55275537958119	39.0	35.0	41.0	33.0	41.0
23	37.52443570995058	39.0	35.0	41.0	33.0	41.0
24	37.446258778648016	39.0	35.0	41.0	33.0	41.0
25	37.32218079760058	39.0	35.0	41.0	32.0	41.0
26	37.13108805461628	39.0	35.0	41.0	32.0	41.0
27	37.09075524471033	39.0	35.0	41.0	32.0	41.0
28	36.9596286555623	39.0	35.0	41.0	31.0	41.0
29	36.936813001551016	39.0	35.0	41.0	31.0	41.0
30	36.80469414821023	39.0	35.0	40.0	31.0	41.0
31	36.641320578403324	39.0	35.0	40.0	31.0	41.0
32	36.55071947182002	39.0	35.0	40.0	30.0	41.0
33	36.37640530620502	39.0	35.0	40.0	30.0	41.0
34	36.22571280855723	39.0	35.0	40.0	30.0	41.0
35	36.15903522377324	39.0	35.0	40.0	30.0	41.0
36	35.928248701867965	38.0	35.0	40.0	29.0	41.0
37	35.826417990488395	38.0	35.0	40.0	29.0	41.0
38	35.653282339302976	38.0	35.0	40.0	27.0	41.0
39	35.66296735161797	38.0	35.0	40.0	27.0	41.0
40	35.477054291944036	38.0	34.0	40.0	27.0	41.0
41	35.386013249456504	38.0	34.0	40.0	26.0	41.0
42	35.384182859198035	38.0	34.0	40.0	27.0	41.0
43	35.20335635771606	38.0	34.0	40.0	26.0	41.0
44	35.05207620845897	38.0	34.0	40.0	26.0	41.0
45	34.87473708210103	38.0	34.0	40.0	24.0	41.0
46	34.74851722333009	37.0	34.0	40.0	24.0	41.0
47	34.571229476347824	37.0	33.0	40.0	23.0	41.0
48	34.474273383235555	37.0	33.0	40.0	23.0	41.0
49	34.330632704899344	37.0	33.0	40.0	23.0	41.0
50	34.13816556361569	37.0	33.0	40.0	23.0	41.0
51	31.921842335963316	35.0	29.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	12.0
10	21.0
11	17.0
12	6.0
13	11.0
14	7.0
15	20.0
16	39.0
17	63.0
18	177.0
19	305.0
20	438.0
21	769.0
22	1204.0
23	1638.0
24	2289.0
25	2954.0
26	3584.0
27	4327.0
28	4919.0
29	5929.0
30	7487.0
31	9381.0
32	12132.0
33	17338.0
34	25022.0
35	29636.0
36	31111.0
37	45875.0
38	55501.0
39	49183.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.182759650491796	20.41045698743453	24.9816158171408	11.42516754493287
2	31.2948566033737	27.96964763381919	23.806633719642015	16.9288620431651
3	32.065547238519116	25.89905879406183	25.072814208966342	16.96257975845271
4	28.71593306551834	26.216005317765383	26.886506170341896	18.181555446374382
5	23.411012526934034	38.85597397634622	20.622718033197497	17.11029546352225
6	25.496694058296328	36.210578371209564	24.67173395759275	13.62099361290136
7	67.16922118500108	20.94705034215453	7.835033669547123	4.048694803297272
8	80.97357494484744	7.034157651191841	7.990135159870139	4.00213224409057
9	74.83534515701216	8.284282085617308	9.890208696601576	6.990164060768957
10	42.06172589745319	26.98765931620473	16.952625004415413	13.997989781926663
11	33.63326043884409	26.036177502898116	23.29733565825008	17.033226400007706
12	30.171896123747228	23.537213118439094	28.370085642996827	17.920805114816847
13	23.853838521044672	26.79627114181029	29.83182888098931	19.51806145615573
14	20.34944397881885	30.244469491890086	28.869107829253487	20.536978700037572
15	19.62692150837002	25.80143798027674	36.143464061732324	18.428176449620917
16	22.142905311021842	24.75490432196886	32.529888346194234	20.57230202081507
17	21.690124562873905	24.326207656169217	29.48630257956578	24.497365201391098
18	22.77776172172288	26.389089589575125	29.577179850293344	21.255968838408652
19	24.691001223471385	27.650453262429796	26.9966507069481	20.661894807150723
20	26.452992688072598	26.600708393142135	27.802664662871013	19.14363425591425
21	23.884987267548468	28.15204441747027	28.16745823017318	19.795510084808082
22	22.618485657126158	25.367924498007444	27.407364591261015	24.606225253605388
23	22.08381902899402	28.179018589700362	27.636966176314747	22.100196204990862
24	22.40172891599151	25.436965534072552	29.60672299130725	22.554582558628685
25	21.29032879589222	28.15043881198039	28.187688859345748	22.371543532781647
26	20.47371784373605	29.580712182371094	27.8395935891384	22.105976384754456
27	21.913624847066078	28.676435170467133	27.489892713441165	21.920047269025623
28	19.542466659602002	27.276347183286287	31.291966513491904	21.889219643619807
29	20.89791881416401	25.651153306423385	30.554672472536122	22.896255406876488
30	21.614982225947227	26.986053710714845	28.90892684540267	22.490037217935253
31	25.326499876368374	27.202810451849498	25.900022157355764	21.570667514426365
32	24.53461524875646	27.365939969621945	25.922821755312146	22.176623026309453
33	23.343255975260828	26.63603171391964	26.828062130510038	23.192650180309496
34	21.8596765026059	27.174230674129525	28.725887819555634	22.240205003708947
35	21.773294927250014	25.015975774624366	28.835068992867903	24.375660305257714
36	22.850977332061696	26.764159032012564	29.801001255583493	20.583862380342254
37	22.59343821148393	27.37172014938554	28.042542123060027	21.992299516070503
38	22.3073193131862	27.39676759502776	27.3919507785581	22.90396231322794
39	22.49292730781705	26.320369674607992	28.28145621995511	22.90524679761985
40	23.64799989724125	25.26773471543854	29.95610274590651	21.128162641413702
41	20.84140150092001	25.95300713852201	29.343403690965904	23.862187669592082
42	21.822426455240535	25.249751933951813	29.524515990225076	23.40330562058258
43	23.707728421465017	24.13064490750107	28.78946979695513	23.372156874078783
44	21.664113753937748	26.044205530347547	28.247096262471537	24.04458445324316
45	21.153531208153904	26.887148412537854	27.33928691848983	24.62003346081841
46	22.904925676521874	27.71178739214345	27.64403084047025	21.739256090864426
47	21.304779245301194	26.346380483544152	30.52545045262019	21.823389818534466
48	22.09955396279491	24.50410874444862	29.32574203057715	24.07059526217932
49	21.52795840839539	23.749474164202063	31.111175335330703	23.611392092071842
50	19.69243021235738	25.603306262824777	29.9506436872409	24.75361983757695
51	20.130118268900386	25.295030008766606	27.971253239309075	26.603598483023934
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	400.0
1	653.0
2	906.0
3	1239.5
4	1573.0
5	1245.5
6	918.0
7	830.5
8	743.0
9	739.5
10	736.0
11	818.0
12	900.0
13	878.5
14	857.0
15	939.5
16	1022.0
17	981.0
18	940.0
19	1122.0
20	1304.0
21	1872.5
22	2441.0
23	2224.5
24	2008.0
25	2352.0
26	3002.0
27	3308.0
28	3873.5
29	4439.0
30	5232.5
31	6026.0
32	6601.0
33	7176.0
34	7849.5
35	8523.0
36	9087.5
37	9652.0
38	10717.5
39	11783.0
40	13640.5
41	15498.0
42	17353.0
43	19208.0
44	21982.0
45	24756.0
46	26812.5
47	28869.0
48	27657.5
49	26446.0
50	25319.5
51	24193.0
52	21542.0
53	18891.0
54	17007.5
55	15124.0
56	14355.5
57	13587.0
58	13379.0
59	13171.0
60	12388.5
61	11606.0
62	10992.5
63	10379.0
64	8768.5
65	7158.0
66	5738.0
67	4318.0
68	3789.5
69	3261.0
70	2924.0
71	2587.0
72	2103.0
73	1619.0
74	1277.5
75	788.0
76	640.0
77	537.0
78	434.0
79	327.5
80	221.0
81	144.0
82	67.0
83	56.0
84	45.0
85	31.5
86	18.0
87	17.5
88	17.0
89	12.0
90	7.0
91	3.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	311409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.75201650864902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58686542286455	28.5163942368658
2	9.022221916638578	6.992585853165463
3	4.10301181372905	4.769999446224302
4	2.5484594395053213	3.950317690853305
5	1.8200354006602555	3.5265020946355916
6	1.2594401021342192	2.928350617773592
7	0.9273008361573881	2.5154344117878598
8	0.7294040667135652	2.2612702747807845
9	0.6052116793069504	2.1107855688957122
>10	5.317740923115709	38.30272542483213
>50	0.05050322009940036	1.2836661602863313
>100	0.02649349251116084	1.7803208006570488
>500	0.0024837649229213286	0.6917231296119296
>1k	8.279216409737762E-4	0.36992428963012747
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG	1153	0.3702526259677787	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC	819	0.26299817924337443	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	776	0.24918997203035234	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	561	0.18014893596524187	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	388	0.12459498601517617	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	312	0.10018978256890457	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.211210979772582E-4	0.0	0.0	0.07161000484892859	0.0
2	3.211210979772582E-4	0.0	0.0	0.4248432126239126	0.0
3	3.211210979772582E-4	0.0	0.0	0.6065977540790407	0.0
4	3.211210979772582E-4	0.0	0.0	0.8898265624949825	0.0
5	3.211210979772582E-4	0.0	0.0	1.910349411866709	0.0
6	3.211210979772582E-4	0.0	0.0	2.3884987267548463	0.0
7	3.211210979772582E-4	0.0	0.0	2.9244498392788905	0.0
8	3.211210979772582E-4	0.0	0.0	3.771888416840875	0.0
9	3.211210979772582E-4	0.0	0.0	4.132507409869336	0.0
10	3.211210979772582E-4	0.0	0.0	5.511401404583682	0.0
11	3.211210979772582E-4	0.0	0.0	6.619911434801178	0.0
12	3.211210979772582E-4	0.0	0.0	7.884807439733598	0.0
13	3.211210979772582E-4	0.0	0.0	8.26854715181642	0.0
14	3.211210979772582E-4	0.0	0.0	8.422043036649551	0.0
15	3.211210979772582E-4	0.0	0.0	8.742200771332877	0.0
16	3.211210979772582E-4	0.0	0.0	9.35907440054719	0.0
17	3.211210979772582E-4	0.0	0.0	10.036318796181227	0.0
18	3.211210979772582E-4	0.0	0.0	10.798660282779238	0.0
19	3.211210979772582E-4	0.0	0.0	11.213869862463834	0.0
20	6.422421959545164E-4	0.0	0.0	11.59728845344868	0.0
21	6.422421959545164E-4	0.0	0.0	12.099200729587135	0.0
22	6.422421959545164E-4	0.0	0.0	12.622306998192089	0.0
23	9.633632939317746E-4	0.0	0.0	13.160827079499951	0.0
24	9.633632939317746E-4	0.0	0.0	13.592092714083408	0.0
25	9.633632939317746E-4	0.0	0.0	13.951106101621983	0.0
26	9.633632939317746E-4	0.0	0.0	14.303054825005058	0.0
27	9.633632939317746E-4	0.0	0.0	14.63059834494186	0.0
28	9.633632939317746E-4	0.0	0.0	14.990896216872345	0.0
29	9.633632939317746E-4	0.0	0.0	15.364681174917873	0.0
30	9.633632939317746E-4	0.0	0.0	15.790487750835718	0.0
31	0.0012844843919090328	0.0	0.0	16.178402037192246	0.0
32	0.0012844843919090328	0.0	0.0	16.52200161202791	0.0
33	0.0012844843919090328	0.0	0.0	16.863995581373693	0.0
34	0.0012844843919090328	0.0	0.0	17.25801116859179	0.0
35	0.0012844843919090328	0.0	0.0	17.668082810708746	0.0
36	0.0012844843919090328	0.0	0.0	18.063704003416728	0.0
37	0.0012844843919090328	0.0	0.0	18.434277750482483	0.0
38	0.0012844843919090328	0.0	0.0	18.8379269706399	0.0
39	0.0012844843919090328	0.0	0.0	19.21235417088138	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	20	7.025357E-4	45.0	11
TTTACGG	20	7.025357E-4	45.0	2
GCGCGAC	25	3.884235E-5	45.0	9
CGTTGAT	20	7.025357E-4	45.0	25
AAGACGT	20	7.025357E-4	45.0	18
CGATTGG	20	7.025357E-4	45.0	2
CGCGACC	25	3.884235E-5	45.0	10
CTACGAG	20	7.025357E-4	45.0	34
TCTCGTC	20	7.025357E-4	45.0	12
AATCTCG	20	7.025357E-4	45.0	10
CGGTGTA	20	7.025357E-4	45.0	32
CGCAGCG	20	7.025357E-4	45.0	15
ATGCGAC	20	7.025357E-4	45.0	11
CGGTCAA	25	3.884235E-5	45.0	22
GACGACC	20	7.025357E-4	45.0	24
ATTGCGG	20	7.025357E-4	45.0	2
CAATCAC	20	7.025357E-4	45.0	42
GGCCTAT	20	7.025357E-4	45.0	8
AGTACGG	45	3.8198777E-10	45.0	2
AGGCGAT	30	2.1603864E-6	44.999996	7
>>END_MODULE