##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936098.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	749271
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.887497314055928	31.0	31.0	34.0	30.0	34.0
2	32.024810782747494	33.0	31.0	34.0	30.0	34.0
3	31.95716770033806	33.0	31.0	34.0	30.0	34.0
4	35.69147878404476	37.0	35.0	37.0	35.0	37.0
5	31.14638228357964	37.0	35.0	37.0	0.0	37.0
6	33.36203322963254	37.0	35.0	37.0	17.0	37.0
7	29.496166273617956	35.0	32.0	37.0	0.0	37.0
8	32.49773579919682	35.0	33.0	37.0	17.0	37.0
9	36.36304754888418	39.0	35.0	39.0	32.0	39.0
10	36.74557936981412	38.0	35.0	39.0	33.0	39.0
11	36.89606163857937	39.0	37.0	39.0	33.0	39.0
12	36.86813983191662	39.0	35.0	39.0	33.0	39.0
13	36.683587113340835	39.0	35.0	39.0	33.0	39.0
14	37.93142668006636	40.0	37.0	41.0	33.0	41.0
15	37.996934353524956	40.0	37.0	41.0	33.0	41.0
16	38.04549221843632	40.0	37.0	41.0	33.0	41.0
17	37.96199105530576	40.0	37.0	41.0	33.0	41.0
18	37.859578176654374	40.0	36.0	41.0	33.0	41.0
19	37.78869461116205	40.0	36.0	41.0	33.0	41.0
20	37.76107576564421	40.0	36.0	41.0	33.0	41.0
21	37.69912354808874	40.0	36.0	41.0	33.0	41.0
22	37.69139470231732	39.0	36.0	41.0	33.0	41.0
23	37.64502963547235	39.0	36.0	41.0	33.0	41.0
24	37.57884263504126	39.0	36.0	41.0	33.0	41.0
25	37.44995335466073	39.0	35.0	41.0	32.0	41.0
26	37.28075422644143	39.0	35.0	41.0	32.0	41.0
27	37.23814080619696	39.0	35.0	41.0	32.0	41.0
28	37.111229448357136	39.0	35.0	41.0	32.0	41.0
29	37.089177347048	39.0	35.0	41.0	32.0	41.0
30	36.96297200879255	39.0	35.0	41.0	31.0	41.0
31	36.8375581064795	39.0	35.0	41.0	31.0	41.0
32	36.75240333604263	39.0	35.0	41.0	31.0	41.0
33	36.59399603080861	39.0	35.0	40.0	30.0	41.0
34	36.46606234593358	39.0	35.0	40.0	30.0	41.0
35	36.369469791303814	39.0	35.0	40.0	30.0	41.0
36	36.26968079640077	39.0	35.0	40.0	30.0	41.0
37	36.21181121383318	39.0	35.0	40.0	30.0	41.0
38	36.10495935382525	39.0	35.0	40.0	29.0	41.0
39	36.105928295636694	39.0	35.0	40.0	29.0	41.0
40	35.95989168138097	38.0	35.0	40.0	29.0	41.0
41	35.86424404521195	38.0	35.0	40.0	29.0	41.0
42	35.83101975119816	38.0	35.0	40.0	29.0	41.0
43	35.71017162014812	38.0	35.0	40.0	28.0	41.0
44	35.55901402830218	38.0	34.0	40.0	27.0	41.0
45	35.42849649859664	38.0	34.0	40.0	27.0	41.0
46	35.29305551662883	38.0	34.0	40.0	26.0	41.0
47	35.1258676767151	38.0	34.0	40.0	26.0	41.0
48	35.029551390618344	38.0	34.0	40.0	26.0	41.0
49	34.9029496670764	38.0	34.0	40.0	26.0	41.0
50	34.73865530629105	37.0	34.0	40.0	24.0	41.0
51	32.62674786559202	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	19.0
9	40.0
10	45.0
11	29.0
12	26.0
13	21.0
14	32.0
15	62.0
16	87.0
17	197.0
18	385.0
19	643.0
20	1050.0
21	1657.0
22	2445.0
23	3337.0
24	4608.0
25	5986.0
26	7708.0
27	9056.0
28	10798.0
29	13193.0
30	16728.0
31	21300.0
32	28057.0
33	40040.0
34	57101.0
35	66415.0
36	74718.0
37	114141.0
38	139708.0
39	129616.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.86944109674604	20.521813869748062	24.304290436971403	11.304454596534498
2	32.32715532831245	25.891299676619006	24.951853201311675	16.829691793756865
3	32.14431093689733	26.025696977462093	26.027832386412925	15.80215969922765
4	28.6377025135098	25.706720265431333	27.228599532078512	18.426977688980354
5	23.4427863883695	38.76381175836246	20.973052473671075	16.82034937959697
6	25.35157506429583	36.699805544322416	23.842641714413077	14.105977676968681
7	68.11260545249984	19.751331627675434	7.824805711151239	4.3112572086734975
8	81.80991924150274	6.516734265706267	7.785300645560818	3.8880458472301744
9	76.18178202546208	7.638491280191012	9.409279152669727	6.770447541677177
10	41.4907289885769	26.470662817592032	17.189775128091174	14.8488330657399
11	32.217849082641656	25.085049334619917	24.3885056274699	18.308595955268522
12	30.061086042299785	23.163448204988583	27.898450627343113	18.877015125368523
13	25.178606939278314	24.32511067424203	29.605042768237393	20.891239618242267
14	21.066076226091763	27.42318867272322	28.656654267948444	22.854080833236573
15	20.86067657763346	26.248713749764775	33.24978545813197	19.6408242144698
16	24.585497103184295	24.668511126147948	30.176931977882504	20.569059792785254
17	23.14756610091676	25.20890305376826	28.52265735628364	23.12087348903134
18	24.072998954984246	26.561284234943034	28.471674467582492	20.894042342490234
19	24.94891701400428	28.044459214356355	25.669350608791746	21.337273162847623
20	25.922396569465523	26.87572320295327	27.659418287909183	19.54246193967203
21	25.491177424456573	27.68103930353637	27.129569941983604	19.69821333002345
22	23.558899250071068	25.101732217048305	27.64700622338246	23.692362309498165
23	23.336149403887248	26.91202515511744	28.526527785007026	21.22529765598829
24	24.508488917894862	24.379163213310004	28.59072351659146	22.521624352203677
25	22.925617033089495	26.77816170651206	27.27971588383909	23.016505376559348
26	21.888342135222103	26.456916122471043	27.91833662319775	23.736405119109108
27	22.102416882543167	26.917363677494528	27.824111703242217	23.15610773672009
28	19.993834006654467	27.22766529066252	29.9715323294242	22.806968373258808
29	22.98874506019851	25.906381002334268	27.942493436954052	23.162380500513166
30	23.46507471929382	26.051855737109804	28.784778804998457	21.698290738597915
31	24.480328212355744	24.982015852742197	28.012428080093848	22.525227854808207
32	25.19101900380503	26.17944642192211	27.242346227199505	21.387188347073355
33	23.56664010751784	25.37386339522015	27.380213567587695	23.67928292967431
34	21.90916237249273	25.29645482075244	29.72596030007834	23.06842250667649
35	21.97989779398909	26.388716499103797	28.38932775991597	23.242057946991142
36	22.130844514201137	28.34435070888904	26.774558203907535	22.750246573002293
37	22.85648316830626	28.124003197774904	28.529730898433275	20.48978273548556
38	21.697489960241352	27.892845178847175	26.555545323387662	23.854119537523808
39	23.16264742663202	25.99219774954589	28.01055959726187	22.834595226560218
40	23.085906167461438	25.515334238212876	28.373045266665862	23.02571432765982
41	19.84395499091784	26.810059377715138	28.452856176203266	24.893129455163752
42	20.883899149973775	26.63028463666684	28.184195037576526	24.301621175782863
43	22.3449192615222	24.769676125193687	27.410776608196503	25.474628005087613
44	21.639033140212284	25.50599182405298	28.1555004797997	24.699474555935037
45	21.78410748580954	25.16125674155279	28.101981792969433	24.952653979668238
46	22.91667500810788	27.059902224962663	27.02426758809563	22.999155178833828
47	21.091968059620616	24.85963022724755	30.236323039327562	23.812078673804272
48	21.069546265636866	24.863500655970938	29.57474665374744	24.492206424644756
49	21.634495396191767	24.404387731541725	29.937232323151434	24.023884549115074
50	20.147449988055055	24.4505659501035	30.19828606739084	25.203697994450607
51	20.128765159735263	24.138662780222376	29.09561427040417	26.636957789638195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	707.0
1	1062.0
2	1417.0
3	2446.0
4	3475.0
5	2669.5
6	1864.0
7	1622.5
8	1381.0
9	1357.5
10	1334.0
11	1332.0
12	1330.0
13	1410.0
14	1490.0
15	1464.5
16	1439.0
17	1521.0
18	1603.0
19	1932.5
20	2262.0
21	2747.0
22	3232.0
23	3403.0
24	3574.0
25	4479.0
26	6430.0
27	7476.0
28	9446.5
29	11417.0
30	13007.5
31	14598.0
32	16341.5
33	18085.0
34	20326.0
35	22567.0
36	24533.0
37	26499.0
38	28107.0
39	29715.0
40	32426.5
41	35138.0
42	38522.5
43	41907.0
44	46835.0
45	51763.0
46	59907.0
47	68051.0
48	63502.5
49	58954.0
50	56819.0
51	54684.0
52	51055.0
53	47426.0
54	44689.0
55	41952.0
56	39497.0
57	37042.0
58	36339.0
59	35636.0
60	34433.0
61	33230.0
62	30419.5
63	27609.0
64	23228.5
65	18848.0
66	15649.0
67	12450.0
68	10300.5
69	8151.0
70	7029.0
71	5907.0
72	4983.5
73	4060.0
74	3296.5
75	1894.0
76	1255.0
77	1001.5
78	748.0
79	597.0
80	446.0
81	361.5
82	277.0
83	226.0
84	175.0
85	134.0
86	93.0
87	64.0
88	35.0
89	31.0
90	27.0
91	20.5
92	14.0
93	11.0
94	8.0
95	4.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	749271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.83508732370542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3732016247652	24.82588684193776
2	8.396277086028844	5.6817753679962575
3	3.6493045563069377	3.70423615000324
4	2.380497946753791	3.2217742360926396
5	1.749338136576097	2.9594504304870184
6	1.3357159281107396	2.7116439040374667
7	1.0684298506428676	2.5305292107028476
8	0.8109356354211644	2.1950462430703714
9	0.6974697336896399	2.1239054410527483
>10	6.421261545055201	44.41505874253356
>50	0.08735952353858864	1.8399270857919061
>100	0.02449332414398155	1.5924119222600102
>500	0.0040822206906635915	0.833291796946365
>1k	0.0016328882762654364	1.3650626270878379
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	3247	0.43335455395978223	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	3079	0.41093275997603007	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC	2233	0.29802301170070644	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1324	0.17670509068147572	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	824	0.10997356096792749	TruSeq Adapter, Index 23 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6692611885419294E-4	0.0	0.0	0.07020156925865274	0.0
2	2.6692611885419294E-4	0.0	0.0	0.37169462050446367	0.0
3	2.6692611885419294E-4	0.0	0.0	0.4962156549499447	0.0
4	2.6692611885419294E-4	0.0	0.0	0.7427219257117919	0.0
5	2.6692611885419294E-4	0.0	0.0	1.706992530072564	0.0
6	2.6692611885419294E-4	0.0	0.0	2.1450182911122946	0.0
7	2.6692611885419294E-4	0.0	0.0	2.5962568950353075	0.0
8	2.6692611885419294E-4	0.0	0.0	3.3002745335132415	0.0
9	2.6692611885419294E-4	0.0	0.0	3.562395982228059	0.0
10	2.6692611885419294E-4	0.0	0.0	4.723524599243798	0.0
11	2.6692611885419294E-4	0.0	0.0	5.403652350084282	0.0
12	2.6692611885419294E-4	0.0	0.0	6.348036958590416	0.0
13	2.6692611885419294E-4	0.0	0.0	6.6056206632847125	0.0
14	2.6692611885419294E-4	0.0	0.0	6.730008234670767	0.0
15	2.6692611885419294E-4	0.0	0.0	6.974779485660061	0.0
16	2.6692611885419294E-4	0.0	0.0	7.377170609832757	0.0
17	2.6692611885419294E-4	0.0	0.0	7.8381520170939485	0.0
18	2.6692611885419294E-4	0.0	0.0	8.329696464963945	0.0
19	2.6692611885419294E-4	0.0	0.0	8.643067728498767	0.0
20	2.6692611885419294E-4	0.0	0.0	8.949231986824527	0.0
21	2.6692611885419294E-4	0.0	0.0	9.336274859163106	0.0
22	2.6692611885419294E-4	0.0	0.0	9.73466209155299	0.0
23	2.6692611885419294E-4	0.0	0.0	10.12303959448584	0.0
24	2.6692611885419294E-4	0.0	0.0	10.445486346061704	0.0
25	2.6692611885419294E-4	0.0	0.0	10.746045155891526	0.0
26	4.0038917828128943E-4	0.0	0.0	11.02952069411468	0.0
27	4.0038917828128943E-4	0.0	0.0	11.32047016366575	0.0
28	4.0038917828128943E-4	0.0	0.0	11.614222357464788	0.0
29	4.0038917828128943E-4	0.0	0.0	11.905972605372423	0.0
30	4.0038917828128943E-4	0.0	0.0	12.242166052069278	0.0
31	5.338522377083859E-4	0.0	0.0	12.568750158487383	0.0
32	5.338522377083859E-4	0.0	0.0	12.892665003716946	0.0
33	5.338522377083859E-4	0.0	0.0	13.20643665643005	0.0
34	5.338522377083859E-4	0.0	0.0	13.525012979282529	0.0
35	5.338522377083859E-4	0.0	0.0	13.876955066991783	0.0
36	5.338522377083859E-4	0.0	0.0	14.190192867467179	0.0
37	5.338522377083859E-4	0.0	0.0	14.513440397399606	0.0
38	5.338522377083859E-4	0.0	0.0	14.859777036612922	0.0
39	5.338522377083859E-4	0.0	0.0	15.209984104549623	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATT	20	7.031712E-4	45.0	42
TACGCTA	20	7.031712E-4	45.0	1
CCGCGAT	20	7.031712E-4	45.0	41
ACATCGC	20	7.031712E-4	45.0	20
TACCGTA	20	7.031712E-4	45.0	18
TACGGGT	60	3.6379788E-12	41.249996	4
GGCGATA	195	0.0	40.384617	8
GTTACGG	85	0.0	39.705883	2
TTGTCGC	40	3.45608E-7	39.375	38
TAGACGG	110	0.0	38.863632	2
TTACGGG	210	0.0	38.57143	3
CGATCAT	35	6.24538E-6	38.571426	10
GCGTAAC	35	6.24538E-6	38.571426	32
CGAATAT	125	0.0	37.800003	14
GCTACGA	125	0.0	37.800003	10
TAACGGG	275	0.0	37.636364	3
CGTGCGG	60	1.546141E-10	37.499996	2
ACACGAC	170	0.0	37.058823	26
CACGACC	170	0.0	37.058823	27
TCAAGCG	170	0.0	37.058823	17
>>END_MODULE