##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936096.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468982
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.836931907834416	31.0	31.0	34.0	30.0	34.0
2	31.96092174113292	33.0	31.0	34.0	30.0	34.0
3	31.906508138905117	33.0	31.0	34.0	30.0	34.0
4	35.653570925963045	37.0	35.0	37.0	33.0	37.0
5	31.145205999377374	37.0	35.0	37.0	0.0	37.0
6	33.352130785403276	37.0	35.0	37.0	17.0	37.0
7	29.55909821698914	35.0	32.0	37.0	0.0	37.0
8	32.53964331253651	35.0	33.0	37.0	17.0	37.0
9	36.3567237122107	39.0	35.0	39.0	32.0	39.0
10	36.720637465830244	38.0	35.0	39.0	33.0	39.0
11	36.86192220596953	39.0	37.0	39.0	33.0	39.0
12	36.832102298169225	39.0	35.0	39.0	33.0	39.0
13	36.56690875129536	39.0	35.0	39.0	32.0	39.0
14	37.80610769709712	40.0	37.0	41.0	33.0	41.0
15	37.894189968911384	40.0	37.0	41.0	33.0	41.0
16	37.96909689497678	40.0	37.0	41.0	33.0	41.0
17	37.929615635568105	40.0	36.0	41.0	33.0	41.0
18	37.8540178514314	40.0	36.0	41.0	33.0	41.0
19	37.75139131139361	40.0	36.0	41.0	33.0	41.0
20	37.712012401328835	39.0	36.0	41.0	33.0	41.0
21	37.64590325428268	39.0	36.0	41.0	33.0	41.0
22	37.604191632088224	39.0	36.0	41.0	33.0	41.0
23	37.55486777744135	39.0	36.0	41.0	33.0	41.0
24	37.48452179401342	39.0	35.0	41.0	33.0	41.0
25	37.37555812376594	39.0	35.0	41.0	32.0	41.0
26	37.18061034325411	39.0	35.0	41.0	32.0	41.0
27	37.17224115211245	39.0	35.0	41.0	32.0	41.0
28	37.05590619682632	39.0	35.0	41.0	32.0	41.0
29	37.02060207001548	39.0	35.0	41.0	31.0	41.0
30	36.92893543888678	39.0	35.0	41.0	31.0	41.0
31	36.774564908674535	39.0	35.0	40.0	31.0	41.0
32	36.675384129881316	39.0	35.0	40.0	31.0	41.0
33	36.548590351015605	39.0	35.0	40.0	30.0	41.0
34	36.37406339688943	39.0	35.0	40.0	30.0	41.0
35	36.29768093444951	39.0	35.0	40.0	30.0	41.0
36	36.16883163959385	39.0	35.0	40.0	30.0	41.0
37	36.03950684674465	38.0	35.0	40.0	29.0	41.0
38	35.90725017164838	38.0	35.0	40.0	29.0	41.0
39	35.859103334456336	38.0	35.0	40.0	29.0	41.0
40	35.768509239160565	38.0	35.0	40.0	28.0	41.0
41	35.703585638681226	38.0	35.0	40.0	28.0	41.0
42	35.66796593472671	38.0	35.0	40.0	28.0	41.0
43	35.506671897855355	38.0	34.0	40.0	27.0	41.0
44	35.23432455829861	38.0	34.0	40.0	26.0	41.0
45	35.13741465557313	38.0	34.0	40.0	26.0	41.0
46	35.003260253058755	38.0	34.0	40.0	25.0	41.0
47	34.855617059929806	38.0	34.0	40.0	24.0	41.0
48	34.75096698807204	38.0	34.0	40.0	24.0	41.0
49	34.5808239975095	37.0	34.0	40.0	24.0	41.0
50	34.40407307743154	37.0	33.0	40.0	23.0	41.0
51	32.24941895424558	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	16.0
10	20.0
11	16.0
12	12.0
13	10.0
14	18.0
15	39.0
16	67.0
17	137.0
18	241.0
19	439.0
20	769.0
21	1099.0
22	1657.0
23	2283.0
24	3169.0
25	4253.0
26	5135.0
27	6124.0
28	7196.0
29	8634.0
30	10833.0
31	13752.0
32	18124.0
33	25230.0
34	35709.0
35	42798.0
36	46958.0
37	71298.0
38	85070.0
39	77859.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08190079789843	20.153225496927387	23.431602918662122	11.333270786512061
2	31.628719225897793	26.419350849286328	24.96001978753982	16.991910137276058
3	32.74048897399047	26.266679744638388	24.739755470359203	16.253075811011936
4	28.233279742079652	26.329368717775946	27.144965051963617	18.292386488180785
5	22.385507332904034	39.351403678606	20.99973986208426	17.263349126405707
6	25.12249937097799	37.627883372922625	24.171716611724968	13.07790064437441
7	68.18044189329228	20.193525551087248	7.449539641180259	4.176492914440213
8	80.79926308472393	6.967005130260863	8.071738360960548	4.161993424054654
9	74.61949499127898	8.541265976092898	9.437888874199862	7.401350158428254
10	40.66467369749798	25.656208553846415	18.326289708347016	15.352828040308584
11	32.65882272667181	26.436195845469552	23.023698137668397	17.881283290190243
12	29.518830146999246	24.11158637218486	27.771215099939873	18.598368380876025
13	23.718820764976055	26.430651922675068	30.666422165456243	19.18410514689263
14	20.485007953396934	29.986012256333932	28.414523371899136	21.11445641837
15	20.226362632254542	27.19592649611286	34.68939106404937	17.888319807583233
16	21.877172258210337	26.381822756523704	30.924001347599695	20.817003637666264
17	21.95222844373558	24.808628049690608	29.268287482248784	23.970856024325027
18	22.59766899369272	26.480760455625163	29.26871393784836	21.652856612833755
19	24.86854506143093	27.150508974758093	27.164582009544073	20.8163639542669
20	25.673693233429002	27.100187214008216	27.650741393059864	19.575378159502925
21	24.219692866677185	27.734753146176182	27.772494466738596	20.273059520408033
22	22.318553803770723	26.111023450793418	26.995065908712913	24.575356836722946
23	22.573147796717144	28.35460636015881	27.590397925719962	21.481847917404078
24	22.7134516889774	26.007821195696213	29.44739883407039	21.831328281255995
25	21.917898767969774	28.158223556554407	27.98764131672431	21.93623635875151
26	20.69375796938902	28.436912290876837	28.453757287060057	22.41557245267409
27	21.979948057707972	28.778076770537037	27.77782516173329	21.464150010021708
28	20.020171349859908	28.458661526455174	30.33719844258415	21.18396868110077
29	21.21318088967167	28.060565224251675	28.877014469638496	21.849239416438156
30	21.861180173226263	27.86674115424473	28.115577996596887	22.156500675932126
31	24.379613716517905	27.595302165115076	26.290561258214602	21.734522860152417
32	23.827140487268167	29.852105198067303	25.281354081819774	21.039400232844756
33	24.19773040329906	26.7402586879667	26.46220963704364	22.5998012716906
34	21.972485084715405	27.454358589455456	28.246713093466276	22.326443232362863
35	21.52683898315927	25.96645500253741	27.45904960105079	25.047656413252533
36	23.733533483161402	27.618757223091716	27.76673731614433	20.880971977602552
37	22.184433517704306	28.147562166565027	28.61239877010205	21.055605545628616
38	23.405589127088035	28.512608159801445	25.946198361557588	22.13560435155294
39	22.848638114042757	26.964574333343283	28.587877573126473	21.598909979487484
40	22.97060441552128	25.46387707843798	29.304536208212685	22.26098229782806
41	20.132115944748413	25.908670268794964	29.062309427653936	24.896904358802683
42	21.453488620032328	26.23661462486833	28.421559889292126	23.888336865807215
43	23.236499481856445	25.281567309619557	28.326033834987268	23.155899373536723
44	22.058629115829607	27.067776588440495	27.5466862267635	23.326908068966397
45	21.74752975593946	26.705929012200897	27.08462158462372	24.461919647235927
46	22.716863333774004	28.2859470086272	26.670533197436146	22.32665646016265
47	20.874361915809136	26.221688678883197	30.398821276722778	22.505128128584893
48	21.530677083555446	25.618467233284008	28.9957823541202	23.855073329040348
49	21.111044773573397	25.1999010623009	30.177064364943647	23.51198979918206
50	20.764123143318933	25.568571928133704	29.651884294066722	24.01542063448064
51	21.37757952330793	25.44383366525794	26.943038325564732	26.23554848586939
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	421.0
1	749.5
2	1078.0
3	1501.0
4	1924.0
5	1574.0
6	1224.0
7	1105.0
8	986.0
9	970.0
10	954.0
11	1044.0
12	1134.0
13	1135.0
14	1136.0
15	1123.0
16	1110.0
17	1123.5
18	1137.0
19	1297.0
20	1457.0
21	2061.5
22	2666.0
23	2784.0
24	2902.0
25	3589.0
26	4939.5
27	5603.0
28	6066.5
29	6530.0
30	7754.5
31	8979.0
32	9975.5
33	10972.0
34	12354.5
35	13737.0
36	14493.0
37	15249.0
38	16782.5
39	18316.0
40	20941.0
41	23566.0
42	26392.5
43	29219.0
44	32866.0
45	36513.0
46	41224.5
47	45936.0
48	43841.0
49	41746.0
50	40132.0
51	38518.0
52	34368.5
53	30219.0
54	27587.0
55	24955.0
56	22892.5
57	20830.0
58	19377.0
59	17924.0
60	17204.5
61	16485.0
62	14637.5
63	12790.0
64	10873.5
65	8957.0
66	7388.5
67	5820.0
68	5146.0
69	4472.0
70	3752.0
71	3032.0
72	2725.0
73	2418.0
74	2023.0
75	1253.5
76	879.0
77	700.0
78	521.0
79	430.5
80	340.0
81	259.5
82	179.0
83	127.0
84	75.0
85	75.5
86	76.0
87	64.5
88	53.0
89	42.5
90	32.0
91	18.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	468982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.93827563147531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42811388875745	26.388797960335257
2	9.27285556541791	6.6650087840169725
3	3.9278929881715268	4.234851025795426
4	2.45256991644101	3.5256453465008555
5	1.7158263653254735	3.0831920426409654
6	1.3241399161898069	2.8552383169600737
7	0.9532523112634985	2.3980771015969955
8	0.7169280984678662	2.061212768855012
9	0.59834342462239	1.935308782472429
>10	5.463219301017022	40.752090720676165
>50	0.11192017902269404	2.45546846990961
>100	0.031977194006484	2.2443552161264293
>500	0.0011843405187586665	0.292196065972083
>1k	0.0017765107781379998	1.108557398141719
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	1931	0.4117428813899041	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	1693	0.3609946650404493	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC	1585	0.3379660626633858	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	726	0.15480338264581583	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	647	0.13795838646259345	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	476	0.10149643269890954	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11663560648383094	0.0
2	0.0	0.0	0.0	0.5635610748386932	0.0
3	0.0	0.0	0.0	0.7369152760660324	0.0
4	0.0	0.0	0.0	1.036926790367221	0.0
5	0.0	0.0	0.0	2.146137804862447	0.0
6	0.0	0.0	0.0	2.6423188949682506	0.0
7	0.0	0.0	0.0	3.1962847188165004	0.0
8	0.0	0.0	0.0	4.042585856173584	0.0
9	0.0	0.0	0.0	4.374581540442917	0.0
10	0.0	0.0	0.0	5.744996609677983	0.0
11	0.0	0.0	0.0	6.739704295687255	0.0
12	0.0	0.0	0.0	7.985381102046561	0.0
13	0.0	0.0	0.0	8.350213867483188	0.0
14	0.0	0.0	0.0	8.508428894925604	0.0
15	0.0	0.0	0.0	8.825285405410016	0.0
16	0.0	0.0	0.0	9.414433816223223	0.0
17	0.0	0.0	0.0	10.057528860382702	0.0
18	0.0	0.0	0.0	10.799988059243212	0.0
19	0.0	0.0	0.0	11.226443658818463	0.0
20	0.0	0.0	0.0	11.643517235203056	0.0
21	0.0	0.0	0.0	12.176160279072544	0.0
22	0.0	0.0	0.0	12.714560473536297	0.0
23	0.0	0.0	0.0	13.226093965226811	0.0
24	0.0	0.0	0.0	13.652762792601848	0.0
25	0.0	0.0	0.0	14.043609349612565	0.0
26	0.0	0.0	0.0	14.399486547458112	0.0
27	0.0	0.0	0.0	14.73553355992341	0.0
28	0.0	0.0	0.0	15.111880626548567	0.0
29	0.0	0.0	0.0	15.495690666166292	0.0
30	0.0	0.0	0.0	15.903168991560444	0.0
31	0.0	0.0	0.0	16.329624591135694	0.0
32	0.0	0.0	0.0	16.740941016926023	0.0
33	0.0	0.0	0.0	17.129655295938864	0.0
34	0.0	0.0	0.0	17.520928308549156	0.0
35	0.0	0.0	0.0	17.927553722744157	0.0
36	0.0	0.0	0.0	18.342708248930663	0.0
37	0.0	0.0	0.0	18.737392906337558	0.0
38	0.0	0.0	0.0	19.100519849375882	0.0
39	0.0	0.0	0.0	19.507145263570884	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGT	20	7.0290104E-4	45.0	17
TCCGCCG	20	7.0290104E-4	45.0	13
AAGCGAT	25	3.887261E-5	45.0	43
AAGCGAC	20	7.0290104E-4	45.0	15
ATATCCG	20	7.0290104E-4	45.0	1
TCAATCA	20	7.0290104E-4	45.0	14
TCTACGG	50	2.1827873E-11	45.0	2
CTCGGTA	20	7.0290104E-4	45.0	39
GCTTACG	20	7.0290104E-4	45.0	9
GTCACGC	20	7.0290104E-4	45.0	13
AACGCCG	35	1.2098644E-7	45.0	13
CATACGT	20	7.0290104E-4	45.0	36
ACGTCGC	20	7.0290104E-4	45.0	20
CGTGTCT	40	6.7993824E-9	45.0	18
CTCGAAC	20	7.0290104E-4	45.0	18
CACCGTG	20	7.0290104E-4	45.0	41
TAGTGCG	20	7.0290104E-4	45.0	1
AGCACCG	30	2.1627438E-6	44.999996	39
TCCATCG	30	2.1627438E-6	44.999996	30
GCGATCA	75	0.0	42.000004	9
>>END_MODULE