##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936095.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	497763
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.909326325982445	31.0	31.0	34.0	30.0	34.0
2	32.05395137846726	33.0	31.0	34.0	30.0	34.0
3	31.972173504258052	33.0	31.0	34.0	30.0	34.0
4	35.705612912169045	37.0	35.0	37.0	35.0	37.0
5	31.25058913579354	37.0	35.0	37.0	0.0	37.0
6	33.44069567243849	37.0	35.0	37.0	17.0	37.0
7	29.537820207608842	35.0	32.0	37.0	0.0	37.0
8	32.60730508294108	35.0	33.0	37.0	17.0	37.0
9	36.4673951257928	39.0	35.0	39.0	32.0	39.0
10	36.76548678788902	38.0	35.0	39.0	33.0	39.0
11	36.93481837742058	39.0	37.0	39.0	33.0	39.0
12	36.95590471770702	39.0	37.0	39.0	33.0	39.0
13	36.740868646323655	39.0	35.0	39.0	33.0	39.0
14	37.99477060368087	40.0	37.0	41.0	33.0	41.0
15	38.11139839642561	40.0	37.0	41.0	33.0	41.0
16	38.14595701167021	40.0	37.0	41.0	33.0	41.0
17	38.11522150099545	40.0	37.0	41.0	33.0	41.0
18	38.03692319437162	40.0	37.0	41.0	33.0	41.0
19	37.924355566805886	40.0	37.0	41.0	33.0	41.0
20	37.86741280488907	40.0	36.0	41.0	33.0	41.0
21	37.79753617685525	40.0	36.0	41.0	33.0	41.0
22	37.77224301525023	40.0	36.0	41.0	33.0	41.0
23	37.72414181046	39.0	36.0	41.0	33.0	41.0
24	37.672195402229576	40.0	36.0	41.0	33.0	41.0
25	37.54897611915711	39.0	36.0	41.0	33.0	41.0
26	37.37200836542692	39.0	35.0	41.0	32.0	41.0
27	37.33257996275336	39.0	35.0	41.0	32.0	41.0
28	37.22254366033634	39.0	35.0	41.0	32.0	41.0
29	37.202287032181985	39.0	35.0	41.0	32.0	41.0
30	37.07149989051014	39.0	35.0	41.0	31.0	41.0
31	36.95686903204939	39.0	35.0	41.0	31.0	41.0
32	36.83478482731742	39.0	35.0	41.0	31.0	41.0
33	36.66346634844293	39.0	35.0	41.0	30.0	41.0
34	36.55945701066572	39.0	35.0	41.0	30.0	41.0
35	36.37160254980784	39.0	35.0	41.0	30.0	41.0
36	36.23404109988087	39.0	35.0	40.0	30.0	41.0
37	36.10358745025243	39.0	35.0	40.0	29.0	41.0
38	35.99350895908294	39.0	35.0	40.0	29.0	41.0
39	35.97643858623481	39.0	35.0	40.0	28.0	41.0
40	35.76817079614194	38.0	35.0	40.0	27.0	41.0
41	35.631069404515806	38.0	35.0	40.0	27.0	41.0
42	35.56931511582822	38.0	35.0	40.0	27.0	41.0
43	35.422355619039585	38.0	34.0	40.0	26.0	41.0
44	35.29014008674811	38.0	34.0	40.0	26.0	41.0
45	35.12424587604944	38.0	34.0	40.0	26.0	41.0
46	34.96201605985178	38.0	34.0	40.0	25.0	41.0
47	34.779941458083464	38.0	34.0	40.0	24.0	41.0
48	34.63707025230883	37.0	33.0	40.0	24.0	41.0
49	34.48889330866296	37.0	33.0	40.0	24.0	41.0
50	34.28392025923984	37.0	33.0	40.0	23.0	41.0
51	32.25312849689511	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	17.0
10	30.0
11	14.0
12	20.0
13	15.0
14	9.0
15	22.0
16	49.0
17	124.0
18	216.0
19	443.0
20	688.0
21	1124.0
22	1744.0
23	2399.0
24	3381.0
25	4575.0
26	5581.0
27	6525.0
28	7631.0
29	8910.0
30	11008.0
31	13921.0
32	17859.0
33	25538.0
34	36283.0
35	44293.0
36	49721.0
37	75758.0
38	93065.0
39	86789.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13940570110675	20.530252349009466	25.148313554844375	11.182028395039406
2	32.73365035167339	25.57743343719802	24.74973029333237	16.93918591779622
3	32.28444058718708	24.830692518326995	25.83337853556813	17.051488358917798
4	28.712459543999856	26.633960338554697	25.835789321424052	18.8177907960214
5	23.0123572864998	39.3649186460223	20.85651203484389	16.766212032634005
6	25.10712929647242	36.632493777159006	24.320811309800046	13.939565616568528
7	69.26890909931032	20.277521631780587	6.746986015433047	3.706583253476052
8	83.71433794797926	5.672780017799636	7.042909979247152	3.5699720549739538
9	77.80248833280096	7.7356091151813215	9.142101763288956	5.319800788728773
10	40.719981195870325	26.56585563812497	17.778742092120144	14.935421073884559
11	32.09901097510261	26.534716320819346	23.178299712915585	18.18797299116246
12	29.33665218186165	24.23321942370164	27.778882721295073	18.651245673141638
13	24.329048161474436	27.0883934723955	29.494759554245697	19.087798811884372
14	20.88986123918411	29.799121268555517	28.181885756876262	21.129131735384107
15	20.006308222989656	28.122620604584913	33.14308214953703	18.7279890228884
16	22.974588308090397	25.77170259742086	32.15928062150059	19.09442847298815
17	23.079658391644216	25.7116338498442	28.22548080110414	22.98322695740744
18	24.230005042560414	26.92928160590482	28.01493883635385	20.82577451518092
19	25.21702095173808	28.559173743327648	26.352300191054777	19.871505113879497
20	26.32236626667711	27.035757981207926	27.671401851885335	18.970473900229628
21	24.58881033744975	27.64307511807828	27.292305776042014	20.475808768429957
22	23.03546065095236	27.120336384986427	26.772982322912714	23.0712206411485
23	22.702169506371504	28.364502785462157	27.123349867306327	21.809977840860007
24	22.32367612699216	26.675345495747976	29.07990348820623	21.921074889053628
25	21.690242143349344	28.417339175471056	27.45342663074596	22.43899205043364
26	21.47266871985262	29.913633596711687	27.371259012823373	21.24243867061232
27	22.194498184879148	29.521077299839483	27.66818747074411	20.616237044537257
28	21.073080964233984	28.452496469203215	29.357344760458293	21.11707780610451
29	22.493234732191826	27.777074631903137	28.286554042787433	21.44313659311761
30	22.2129808764412	28.202980133115556	27.7172067831478	21.866832207295438
31	24.779262420067383	28.091883084921943	25.58848287237099	21.54037162263969
32	25.132844345602223	28.98588283982538	25.954721423649406	19.926551390922988
33	23.69842676133019	28.97503430347374	25.256598019539418	22.069940915656648
34	23.47542906965765	28.4010663709436	26.6407908984798	21.48271366091895
35	23.553779609975027	26.931491492939415	27.326659474488864	22.188069422596698
36	24.27299739032431	29.368595094452584	25.6150015167861	20.743405998437005
37	23.481054236654796	29.49576404835233	27.340923290803055	19.682258424189826
38	23.563623652220034	28.089874096708673	25.59671972404538	22.749782527025914
39	23.565431741611974	27.56311738719029	26.848118482088868	22.023332389108873
40	23.9294604058558	26.237386065255958	28.42195984836157	21.411193680526676
41	21.538161735605097	27.32585587920356	28.48705106647139	22.648931318719953
42	22.969565837557234	27.892189656523286	27.652718261501956	21.485526244417525
43	23.314910911417684	27.770444970799357	26.913611497841345	22.00103261994162
44	23.131490287546484	27.543831100342935	26.854547244371318	22.470131367739267
45	22.62341716841147	27.817656193811114	25.942868393191137	23.61605824458628
46	23.03666604388032	28.380775589989614	26.435472303083994	22.147086063046068
47	22.665405022068736	27.761002726197002	28.348430879756027	21.22516137197823
48	22.984834147978052	26.90537464616695	28.12683947983277	21.982951726022222
49	22.168783135749344	26.643000785514392	28.332158075228577	22.85605800350769
50	21.31837842507378	26.752691541958722	28.430598497678616	23.498331535288884
51	21.561265100057657	26.358528054515908	27.279247352655783	24.800959492770655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	553.0
1	907.5
2	1262.0
3	1768.5
4	2275.0
5	1805.5
6	1336.0
7	1157.0
8	978.0
9	1033.5
10	1089.0
11	1161.0
12	1233.0
13	1284.5
14	1336.0
15	1279.5
16	1223.0
17	1213.0
18	1203.0
19	1324.5
20	1446.0
21	1926.5
22	2407.0
23	2469.0
24	2531.0
25	3238.0
26	4924.5
27	5904.0
28	6984.5
29	8065.0
30	9096.0
31	10127.0
32	11120.0
33	12113.0
34	13647.0
35	15181.0
36	16679.0
37	18177.0
38	19079.0
39	19981.0
40	22930.0
41	25879.0
42	28074.0
43	30269.0
44	32295.5
45	34322.0
46	35607.5
47	36893.0
48	38638.5
49	40384.0
50	39951.0
51	39518.0
52	38393.0
53	37268.0
54	34662.5
55	32057.0
56	28629.5
57	25202.0
58	23746.5
59	22291.0
60	20415.0
61	18539.0
62	16608.0
63	14677.0
64	12170.5
65	9664.0
66	8119.0
67	6574.0
68	5206.5
69	3839.0
70	3375.5
71	2912.0
72	2581.0
73	2250.0
74	1750.0
75	964.5
76	679.0
77	533.5
78	388.0
79	333.5
80	279.0
81	208.5
82	138.0
83	97.0
84	56.0
85	39.5
86	23.0
87	23.0
88	23.0
89	20.5
90	18.0
91	11.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	497763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.08865275098852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.98832105397007	28.9210378930729
2	8.710436388027318	6.809584465623492
3	4.080816947038398	4.785409097493992
4	2.5621844396416265	4.006093513805506
5	1.8037176858619794	3.525244714173776
6	1.2631053381784147	2.9623851571185593
7	0.9983671332049283	2.7317378331493143
8	0.7673355618504996	2.3995290656527084
9	0.6022096132513955	2.1185606210121812
>10	5.13869603404464	38.52496935940602
>50	0.06641731711446501	1.658948441571727
>100	0.01788158537695503	1.3889474432554239
>500	5.109024393415724E-4	0.16755239466445732
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	839	0.1685541110930302	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05926515229135151	0.0
2	0.0	0.0	0.0	0.33469743633014104	0.0
3	0.0	0.0	0.0	0.49642098749806635	0.0
4	0.0	0.0	0.0	0.7015386840725405	0.0
5	0.0	0.0	0.0	1.318498964366576	0.0
6	0.0	0.0	0.0	1.743600870293694	0.0
7	0.0	0.0	0.0	2.134549976595287	0.0
8	0.0	0.0	0.0	2.821423046710985	0.0
9	0.0	0.0	0.0	3.0906274672886496	0.0
10	0.0	0.0	0.0	3.887593091491332	0.0
11	0.0	0.0	0.0	4.810924074308456	0.0
12	0.0	0.0	0.0	5.609898686724405	0.0
13	0.0	0.0	0.0	5.906626245823816	0.0
14	0.0	0.0	0.0	6.0293754256543775	0.0
15	0.0	0.0	0.0	6.261815361929272	0.0
16	0.0	0.0	0.0	6.7773217374533665	0.0
17	0.0	0.0	0.0	7.36233910515647	0.0
18	0.0	0.0	0.0	7.996576684084594	0.0
19	0.0	0.0	0.0	8.37085118821608	0.0
20	0.0	0.0	0.0	8.730861876033373	0.0
21	0.0	0.0	0.0	9.198956129724387	0.0
22	0.0	0.0	0.0	9.702810373611538	0.0
23	0.0	0.0	0.0	10.204856528106749	0.0
24	0.0	0.0	0.0	10.613082933042431	0.0
25	0.0	0.0	0.0	10.94074891062614	0.0
26	0.0	0.0	0.0	11.265602304711278	0.0
27	0.0	0.0	0.0	11.592062889367028	0.0
28	0.0	0.0	0.0	11.941225040832686	0.0
29	0.0	0.0	0.0	12.302039323935286	0.0
30	0.0	0.0	0.0	12.696604609020758	0.0
31	0.0	0.0	0.0	13.084540233002453	0.0
32	0.0	0.0	0.0	13.44917159371026	0.0
33	0.0	0.0	0.0	13.800342733389183	0.0
34	0.0	0.0	0.0	14.172608249307402	0.0
35	0.0	0.0	0.0	14.560342974467769	0.0
36	0.0	0.0	0.0	14.938635455025786	0.0
37	0.0	0.0	0.0	15.325164787258194	0.0
38	0.0	0.0	0.0	15.706872547778762	0.0
39	0.0	0.0	0.0	16.10706299986138	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	20	7.0294284E-4	45.0	8
CGATTAT	20	7.0294284E-4	45.0	12
CGCGAAG	20	7.0294284E-4	45.0	2
ATCCGTT	20	7.0294284E-4	45.0	11
TTAACGG	40	6.8012014E-9	45.0	2
CGTACAC	20	7.0294284E-4	45.0	25
CCGAACT	25	3.887608E-5	45.0	13
AATACGG	35	1.2100645E-7	45.0	2
GCGTACA	20	7.0294284E-4	45.0	24
CATACGA	140	0.0	43.392857	18
TGCTCGG	70	0.0	41.785713	2
TATGCGG	60	3.6379788E-12	41.249996	2
TTACGGG	160	0.0	40.78125	3
GCGAGAC	50	1.0786607E-9	40.5	21
ACATACG	150	0.0	40.5	17
GTATGCG	40	3.4529512E-7	39.375	1
CTACGAA	40	3.4529512E-7	39.375	11
GCTACGA	40	3.4529512E-7	39.375	10
GCGATGA	115	0.0	39.130432	9
AGTACGG	75	0.0	39.0	2
>>END_MODULE