##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936094.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323311
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86177395758264	31.0	31.0	34.0	30.0	34.0
2	31.993059314406253	33.0	31.0	34.0	30.0	34.0
3	31.934267624670984	33.0	31.0	34.0	30.0	34.0
4	35.67905824422924	37.0	35.0	37.0	33.0	37.0
5	31.161074012328687	37.0	35.0	37.0	0.0	37.0
6	33.377518859550094	37.0	35.0	37.0	17.0	37.0
7	29.522020593174993	35.0	32.0	37.0	0.0	37.0
8	32.54675838434201	35.0	33.0	37.0	17.0	37.0
9	36.362737426193355	39.0	35.0	39.0	32.0	39.0
10	36.75338915162181	38.0	35.0	39.0	33.0	39.0
11	36.90363458094528	39.0	37.0	39.0	33.0	39.0
12	36.88694476834998	39.0	35.0	39.0	33.0	39.0
13	36.69096009724383	39.0	35.0	39.0	33.0	39.0
14	37.94812115888417	40.0	37.0	41.0	33.0	41.0
15	38.061674363074566	40.0	37.0	41.0	33.0	41.0
16	38.11549251340041	40.0	37.0	41.0	33.0	41.0
17	38.06150115523443	40.0	37.0	41.0	33.0	41.0
18	37.96908549353409	40.0	37.0	41.0	33.0	41.0
19	37.90274379776748	40.0	37.0	41.0	33.0	41.0
20	37.848542115795624	40.0	36.0	41.0	33.0	41.0
21	37.77100377036352	40.0	36.0	41.0	33.0	41.0
22	37.76819842195285	40.0	36.0	41.0	33.0	41.0
23	37.699181283655676	39.0	36.0	41.0	33.0	41.0
24	37.66618519011107	39.0	36.0	41.0	33.0	41.0
25	37.54119098948072	39.0	36.0	41.0	33.0	41.0
26	37.35614934227416	39.0	35.0	41.0	32.0	41.0
27	37.31708478833074	39.0	35.0	41.0	32.0	41.0
28	37.17227375499132	39.0	35.0	41.0	32.0	41.0
29	37.12381886171519	39.0	35.0	41.0	32.0	41.0
30	37.02814318102384	39.0	35.0	41.0	31.0	41.0
31	36.87915041554417	39.0	35.0	41.0	31.0	41.0
32	36.70096903600558	39.0	35.0	41.0	30.0	41.0
33	36.576732619675795	39.0	35.0	41.0	30.0	41.0
34	36.42735322955297	39.0	35.0	40.0	30.0	41.0
35	36.260761310317314	39.0	35.0	40.0	30.0	41.0
36	36.180216571660104	39.0	35.0	40.0	30.0	41.0
37	36.017756896610386	39.0	35.0	40.0	29.0	41.0
38	35.8928152769315	39.0	35.0	40.0	28.0	41.0
39	35.85283210283597	39.0	35.0	40.0	28.0	41.0
40	35.691628184627184	38.0	35.0	40.0	27.0	41.0
41	35.61925205143036	38.0	35.0	40.0	27.0	41.0
42	35.53959809595096	38.0	34.0	40.0	26.0	41.0
43	35.383073882422806	38.0	34.0	40.0	26.0	41.0
44	35.20502859475861	38.0	34.0	40.0	25.0	41.0
45	35.062651750172435	38.0	34.0	40.0	25.0	41.0
46	34.92429270887783	38.0	34.0	40.0	24.0	41.0
47	34.74609586435352	38.0	34.0	40.0	23.0	41.0
48	34.62720724008772	38.0	33.0	40.0	23.0	41.0
49	34.46262576899642	37.0	33.0	40.0	23.0	41.0
50	34.29375121786762	37.0	33.0	40.0	22.0	41.0
51	32.22939831926535	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	15.0
10	16.0
11	6.0
12	11.0
13	5.0
14	15.0
15	12.0
16	49.0
17	79.0
18	168.0
19	277.0
20	478.0
21	834.0
22	1232.0
23	1729.0
24	2403.0
25	3017.0
26	3726.0
27	4389.0
28	5265.0
29	5963.0
30	7323.0
31	9266.0
32	11857.0
33	16487.0
34	23210.0
35	28185.0
36	31801.0
37	48283.0
38	59830.0
39	57366.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.30436638406983	20.228819928799204	23.196550689583713	11.270262997547253
2	32.123249750240475	25.571972497069385	24.499321087126635	17.805456665563497
3	31.904265552362894	26.14417696892466	25.6629066131372	16.288650865575253
4	27.754700582411363	26.72411393364284	26.683904970755712	18.837280513190084
5	22.50866812449932	39.865330904299576	20.946395266477168	16.679605704723937
6	25.806730980387304	36.89326994751184	24.056094596224685	13.24390447587617
7	67.9537658786741	20.3686233997606	7.588359195944462	4.089251525620842
8	81.28953236976162	6.8178936070842004	7.941270170207633	3.951303852946544
9	74.37390005289025	9.314870202374802	9.69530885122993	6.615920893505016
10	39.53778250662675	29.60647797322083	17.53574731450523	13.319992205647194
11	30.878936998741153	26.665656287599248	25.1318390033126	17.323567710347003
12	28.66929983823625	25.072144158410943	28.116271948680993	18.142284054671816
13	23.88845415095682	26.748548611089635	30.387459752374646	18.9755374855789
14	19.362162128724357	30.34230199405526	28.80879400948313	21.486741867737255
15	18.790576256298117	28.429283259771555	34.40433514479866	18.375805339131674
16	21.554787804930857	27.078262106764072	31.701364939640158	19.665585148664906
17	21.85388062886818	26.396256236255493	29.20407904463504	22.545784090241288
18	22.33855328151532	27.431482380741762	29.316664140718995	20.91330019702392
19	23.345942451695116	28.559807739297455	26.660398192452465	21.433851616554957
20	25.412373844378944	27.326629777520715	28.50258729211193	18.758409085988415
21	24.40529397391366	28.08132108094064	28.365258218866664	19.14812672627903
22	23.11551416438043	25.86116773014219	27.283327817488424	23.739990287988963
23	22.16441754224261	28.20658746531977	28.913955912418693	20.71503908001893
24	22.382164541262128	25.874467617866387	29.441311925669094	22.30205591520239
25	21.00485291252076	28.762708352020187	27.567264955414444	22.6651737800446
26	21.038875881117562	29.591322287209533	27.547160473970884	21.82264135770203
27	21.831611049423003	29.38811237477228	27.99317066230348	20.787105913501243
28	19.87652755396507	28.96437176588486	30.237140091119695	20.921960589030377
29	22.544237591668704	26.071182236298796	29.944542561187216	21.440037610845287
30	22.061730037023175	26.536059707216893	28.86787025495575	22.53434000080418
31	24.739956265020368	26.896084574913935	26.523687718636236	21.840271441429458
32	24.450142432518536	28.673011434810448	27.0485693341705	19.828276798500514
33	22.300509416629808	28.67084633680883	26.931035442654288	22.09760880390707
34	22.270198044607206	27.250542047749686	29.40759825678681	21.071661650856296
35	21.644175422426084	27.845325398764658	27.562934759411217	22.94756441939804
36	23.130669850391726	29.621324359517615	26.39903993368614	20.848965856404515
37	21.614173350117998	29.338624420449662	28.900965324409007	20.146236905023336
38	21.30858523217583	28.805701012337963	27.439833473033705	22.4458802824525
39	21.870273513737548	26.768962392247715	28.928183699286446	22.432580394728298
40	22.418971207289577	27.16857762340285	29.37852408362227	21.0339270856853
41	19.96746167003288	28.01946113803737	29.117165824855945	22.895911367073808
42	21.96863082295375	27.637785290324178	28.154625113281018	22.238958773441052
43	23.15696032612562	26.950830624383332	27.49117722564342	22.401031823847624
44	21.6311848344164	27.99317066230348	27.370859636696554	23.00478486658357
45	21.117128708890203	27.488393528212775	27.434575377886926	23.9599023850101
46	22.088948411900617	28.392167294029587	27.330341374094914	22.188542919974886
47	21.325906016188746	27.368694538694943	29.35996610075129	21.94543334436502
48	21.391477555666217	27.32199028180297	28.47475031780546	22.811781844725356
49	20.56502871847849	26.40089573197324	30.16661975620997	22.867455793338305
50	19.54712335800514	26.556164188660453	29.962791244343684	23.933921208990725
51	20.338312027738	26.288001336174766	27.84408819990659	25.529598436180645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	372.0
1	599.0
2	826.0
3	1069.5
4	1313.0
5	1079.5
6	846.0
7	743.0
8	640.0
9	668.0
10	696.0
11	692.5
12	689.0
13	734.0
14	779.0
15	779.0
16	779.0
17	865.5
18	952.0
19	1010.0
20	1068.0
21	1511.5
22	1955.0
23	1973.5
24	1992.0
25	2620.5
26	3742.5
27	4236.0
28	5242.0
29	6248.0
30	7187.5
31	8127.0
32	8943.0
33	9759.0
34	10455.5
35	11152.0
36	11575.5
37	11999.0
38	12902.0
39	13805.0
40	15381.0
41	16957.0
42	19065.0
43	21173.0
44	23339.5
45	25506.0
46	27383.0
47	29260.0
48	27701.5
49	26143.0
50	24902.0
51	23661.0
52	21249.0
53	18837.0
54	17043.5
55	15250.0
56	14243.5
57	13237.0
58	13068.5
59	12900.0
60	12641.0
61	12382.0
62	10726.5
63	9071.0
64	7486.5
65	5902.0
66	4954.0
67	4006.0
68	3307.5
69	2609.0
70	2144.0
71	1679.0
72	1394.5
73	1110.0
74	939.5
75	741.0
76	713.0
77	535.5
78	358.0
79	259.5
80	161.0
81	116.5
82	72.0
83	55.0
84	38.0
85	29.0
86	20.0
87	13.5
88	7.0
89	4.5
90	2.0
91	3.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	323311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.51318661336304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41219633334907	28.273376170831188
2	8.721701827119373	6.718010601079157
3	4.216941151712201	4.872235245403862
4	2.573879406163752	3.9651319155950646
5	1.8083977090118262	3.4823579219175325
6	1.3131680428921804	3.034457153436676
7	1.0319980899899355	2.782187451709162
8	0.7711441458185148	2.3759374714968717
9	0.6194735782590272	2.14721113693839
>10	5.448450190581295	38.72901790416186
>50	0.06178653818367293	1.6542831080616474
>100	0.018455719197720485	1.310941330916777
>500	0.0024072677214418024	0.6548525884518271
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG	887	0.2743488467760144	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGC	664	0.20537501043886536	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTC	568	0.17568223784529446	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	433	0.1339267763855854	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	351	0.10856419979524358	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	342	0.10578050236459632	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10330610464846542	0.0
2	0.0	0.0	0.0	0.509725929522967	0.0
3	0.0	0.0	0.0	0.680768671650516	0.0
4	0.0	0.0	0.0	0.9418176307023268	0.0
5	0.0	0.0	0.0	1.8381682033707483	0.0
6	0.0	0.0	0.0	2.249536823677512	0.0
7	0.0	0.0	0.0	2.7342094763246534	0.0
8	0.0	0.0	0.0	3.508077362044595	0.0
9	0.0	0.0	0.0	3.770982119383504	0.0
10	0.0	0.0	0.0	4.85662411733594	0.0
11	0.0	0.0	0.0	5.687712450241408	0.0
12	0.0	0.0	0.0	6.688297026701845	0.0
13	0.0	0.0	0.0	6.990482847784332	0.0
14	0.0	0.0	0.0	7.124100324455401	0.0
15	0.0	0.0	0.0	7.382056286362047	0.0
16	0.0	0.0	0.0	7.895803112173727	0.0
17	0.0	0.0	0.0	8.470481981745131	0.0
18	0.0	0.0	0.0	9.11784628422788	0.0
19	0.0	0.0	0.0	9.454364373621683	0.0
20	0.0	0.0	0.0	9.82212173418164	0.0
21	0.0	0.0	0.0	10.261945928223907	0.0
22	0.0	0.0	0.0	10.784353146042045	0.0
23	0.0	0.0	0.0	11.2900581792763	0.0
24	3.092997145163635E-4	0.0	0.0	11.669568928987879	0.0
25	3.092997145163635E-4	0.0	0.0	11.999901024091354	0.0
26	3.092997145163635E-4	0.0	0.0	12.327140122049668	0.0
27	3.092997145163635E-4	0.0	0.0	12.646028127716038	0.0
28	3.092997145163635E-4	0.0	0.0	12.957492940234015	0.0
29	3.092997145163635E-4	0.0	0.0	13.266483355035863	0.0
30	3.092997145163635E-4	0.0	0.0	13.655582395897449	0.0
31	3.092997145163635E-4	0.0	0.0	14.024267655600953	0.0
32	3.092997145163635E-4	0.0	0.0	14.39790171073672	0.0
33	3.092997145163635E-4	0.0	0.0	14.728543105554714	0.0
34	3.092997145163635E-4	0.0	0.0	15.06134959837432	0.0
35	3.092997145163635E-4	0.0	0.0	15.447046342376225	0.0
36	3.092997145163635E-4	0.0	0.0	15.811092106361986	0.0
37	3.092997145163635E-4	0.0	0.0	16.185963360355817	0.0
38	3.092997145163635E-4	0.0	0.0	16.569804306070626	0.0
39	3.092997145163635E-4	0.0	0.0	16.948077856924137	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGGGT	30	2.1606447E-6	45.000004	4
TACCCGT	20	7.025757E-4	45.0	35
GCGAATG	25	3.8845657E-5	45.0	1
CCAGGCG	20	7.025757E-4	45.0	17
ATTACGG	20	7.025757E-4	45.0	2
GGGTACG	20	7.025757E-4	45.0	7
CACGACC	25	3.8845657E-5	45.0	27
GTTACAA	35	1.2083001E-7	45.0	9
AGCGATG	45	3.8380676E-10	45.0	1
CATACGG	25	3.8845657E-5	45.0	2
ACGCTCA	20	7.025757E-4	45.0	26
TATAGCG	35	1.2083001E-7	45.0	1
ATGCGCG	20	7.025757E-4	45.0	16
CCGAATG	25	3.8845657E-5	45.0	37
ACCCGTC	20	7.025757E-4	45.0	36
TAGGCGT	20	7.025757E-4	45.0	45
CGTTTTA	20	7.025757E-4	45.0	1
AATACGG	20	7.025757E-4	45.0	2
TACGGGA	90	0.0	42.5	4
GCGATGA	90	0.0	42.5	9
>>END_MODULE