FastQCFastQC Report
Sat 14 Jan 2017
SRR2936093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519389
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7490.1442079058278092No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG7370.14189749879184965No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC5920.11398008044067165No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC5920.11398008044067165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG302.163195E-645.0000041
CGTTGCG207.029712E-445.031
TTGTACG253.887842E-545.01
ATGACGG502.1827873E-1145.02
GCGATAC253.887842E-545.09
TAGGCGA253.887842E-545.06
TATCGTT207.029712E-445.040
TAGCTAC207.029712E-445.029
TGTAACG207.029712E-445.01
CTACGAA501.0786607E-940.511
CGAATAT501.0786607E-940.514
CGTTATT1350.040.01
AACGGGA1600.039.3754
CACGACC1150.039.13043627
CTAACGG356.2416784E-638.5714262
TATACGG356.2416784E-638.5714262
TAACTCG356.2416784E-638.57142638
GCGAGAC1200.037.50000421
ACACGAC1200.037.50000426
TTCACGG601.546141E-1037.5000042
Produced by FastQC (version 0.11.5)