##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936093.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519389
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.897464135744116	31.0	31.0	34.0	30.0	34.0
2	32.041383240692426	33.0	31.0	34.0	30.0	34.0
3	31.98031148137523	33.0	31.0	34.0	30.0	34.0
4	35.71136469967597	37.0	35.0	37.0	35.0	37.0
5	31.117609344826324	37.0	35.0	37.0	0.0	37.0
6	33.36547173698326	37.0	35.0	37.0	17.0	37.0
7	29.55228739923256	35.0	32.0	37.0	0.0	37.0
8	32.5824016296071	35.0	33.0	37.0	17.0	37.0
9	36.45019821367029	39.0	35.0	39.0	32.0	39.0
10	36.83396837437836	38.0	37.0	39.0	33.0	39.0
11	36.919828875852204	39.0	37.0	39.0	33.0	39.0
12	36.80831130424402	39.0	35.0	39.0	33.0	39.0
13	36.53776841635075	39.0	35.0	39.0	32.0	39.0
14	37.58450024933143	40.0	36.0	41.0	33.0	41.0
15	37.74293063580476	40.0	36.0	41.0	33.0	41.0
16	37.86090964575684	40.0	36.0	41.0	33.0	41.0
17	37.85629653304171	40.0	36.0	41.0	33.0	41.0
18	37.77147764007324	40.0	36.0	41.0	33.0	41.0
19	37.68363403922686	40.0	36.0	41.0	33.0	41.0
20	37.59205335499982	39.0	36.0	41.0	33.0	41.0
21	37.481662106821666	39.0	35.0	41.0	32.0	41.0
22	37.5034299917788	39.0	35.0	41.0	33.0	41.0
23	37.45389871560622	39.0	35.0	41.0	33.0	41.0
24	37.3799733918123	39.0	35.0	41.0	32.0	41.0
25	37.238212592103416	39.0	35.0	41.0	32.0	41.0
26	37.070326864835415	39.0	35.0	41.0	32.0	41.0
27	37.015766602681225	39.0	35.0	41.0	32.0	41.0
28	36.90769538823502	39.0	35.0	41.0	31.0	41.0
29	36.84563785524915	39.0	35.0	41.0	31.0	41.0
30	36.696347053942226	39.0	35.0	40.0	31.0	41.0
31	36.54817680004775	39.0	35.0	40.0	30.0	41.0
32	36.42925437388932	39.0	35.0	40.0	30.0	41.0
33	36.25356909753576	39.0	35.0	40.0	30.0	41.0
34	36.04646421083234	38.0	35.0	40.0	29.0	41.0
35	35.934369037465174	38.0	35.0	40.0	29.0	41.0
36	35.76966397055001	38.0	35.0	40.0	28.0	41.0
37	35.62737947857964	38.0	34.0	40.0	27.0	41.0
38	35.42718463425294	38.0	34.0	40.0	27.0	41.0
39	35.389788771036734	38.0	34.0	40.0	26.0	41.0
40	35.21996422719773	38.0	34.0	40.0	26.0	41.0
41	35.076805631232084	38.0	34.0	40.0	25.0	41.0
42	35.063424523815485	38.0	34.0	40.0	25.0	41.0
43	34.93732250779281	38.0	34.0	40.0	25.0	41.0
44	34.75914391717961	38.0	34.0	40.0	24.0	41.0
45	34.60599281078344	37.0	33.0	40.0	24.0	41.0
46	34.53473600711605	37.0	33.0	40.0	23.0	41.0
47	34.35542339171604	37.0	33.0	40.0	23.0	41.0
48	34.22379950287742	37.0	33.0	40.0	23.0	41.0
49	34.100231233237515	36.0	33.0	40.0	23.0	41.0
50	33.91549301198138	36.0	33.0	40.0	23.0	41.0
51	31.839024315108713	35.0	29.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	16.0
9	16.0
10	34.0
11	20.0
12	17.0
13	12.0
14	28.0
15	32.0
16	89.0
17	180.0
18	314.0
19	628.0
20	982.0
21	1515.0
22	2254.0
23	3119.0
24	4136.0
25	5457.0
26	6534.0
27	7871.0
28	8772.0
29	10276.0
30	12730.0
31	16076.0
32	20444.0
33	29113.0
34	41137.0
35	46848.0
36	52372.0
37	78214.0
38	90254.0
39	79890.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.323019933036704	20.406670145112816	25.201919948246882	11.0683899736036
2	32.438114784872226	23.934661689023063	26.025580056566465	17.601643469538246
3	32.55170979747357	24.605449865129987	25.9493366243798	16.893503713016642
4	28.82155763791686	26.492859879589286	25.748716280090644	18.93686620240321
5	21.8797471644567	40.03126750855332	21.229752651673408	16.859232675316573
6	27.070846706418507	34.165144044251996	24.493010056046625	14.270999193282877
7	69.58406897335138	19.450739233984546	6.987441012420363	3.977750780243709
8	82.78746758210129	5.798544058499506	7.504779654555642	3.909208704843576
9	77.60907527883725	7.079472226019419	9.421262290884098	5.890190204259235
10	45.77782740874374	23.62314180700785	15.97068863607046	14.628342148177955
11	33.81608004790244	23.64817121656408	22.909033498976683	19.626715236556798
12	29.65734738317523	22.049947149439053	28.6629096881143	19.629795779271415
13	26.049261728685053	23.757145415093504	30.025472237571453	20.16812061864999
14	20.88607960507442	26.156503121937508	31.391885465421872	21.5655318075662
15	20.445754530804464	24.237902612492757	34.64166549541866	20.674677361284125
16	25.14165683139227	23.865927079703265	29.93536636316903	21.057049725735432
17	25.432190516164184	23.143347279206914	27.145164799408533	24.279297405220365
18	25.837089349216097	23.8526422392465	28.473841379004945	21.836427032532455
19	26.088346114376698	25.069456611518536	26.333249260188413	22.508948013916353
20	26.77742501285164	25.83208346730485	27.453989206548464	19.936502313295044
21	25.74332533034007	25.264300938217794	28.931109438205276	20.06126429323686
22	25.558877835302635	22.738640980074663	28.195052263332492	23.507428921290206
23	23.492796343395796	25.361723101567417	28.220851808567378	22.92462874646941
24	24.296817991909727	24.17513655468252	28.97828024852278	22.54976520488497
25	24.3724838223374	25.38444210408769	27.44782812111924	22.795245952455673
26	23.29910722021452	25.518638246093005	26.83248971387534	24.349764819817132
27	22.680495736336347	25.291255686970654	28.568760601398953	23.459487975294046
28	21.067639091316913	25.713482572792262	29.029109203313897	24.189769132576934
29	22.661819946129008	25.77008754517327	27.79092356595923	23.777168942738484
30	23.975671413911346	24.34264106478959	28.88875197587935	22.792935545419716
31	25.179778547485604	25.55252421595375	26.320156953651306	22.947540282909344
32	26.71215601408578	25.407931242286608	25.550791410676776	22.329121332950834
33	26.09932054779751	24.968761371534633	25.880409481140337	23.05150859952752
34	22.971029421108263	26.13782733173017	28.09320181983061	22.797941427330958
35	23.57712620020832	26.82575102668713	27.186752126055808	22.410370647048744
36	25.823611974839665	26.49151214215164	26.17190583551057	21.51297004749812
37	24.18322297930838	27.87833396548637	26.55812887835514	21.380314176850106
38	24.341100793432283	27.821151391346373	25.635313801408966	22.202434013812383
39	24.764097814932544	25.177660674369307	26.93106708074295	23.127174429955197
40	25.992464222384378	24.986474492143653	26.980355764176757	22.040705521295216
41	21.94886684161582	25.56388371721388	27.5240715533059	24.963177887864394
42	21.533956244741418	25.34092943824378	28.81173840801403	24.31337590900077
43	23.017237561827457	24.316841519554707	28.915706724632212	23.750214193985624
44	23.595994524335325	24.52362294927309	27.525419290743546	24.354963235648043
45	23.10522556311358	25.469542096578863	26.429323686100396	24.995908654207156
46	24.082527739324476	25.732350896919264	26.773381800538708	23.41173956321755
47	22.11983696227683	25.823997042678993	28.80634745826346	23.249818536780715
48	22.153145330378578	26.436832508967267	28.258010855062395	23.152011305591763
49	24.044598557150806	24.797791250873622	28.345421254589525	22.812188937386043
50	22.10173877382848	25.969360152024784	27.741249814686103	24.187651259460637
51	22.426736030220123	25.39791947846412	26.578537473839454	25.5968070174763
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	542.0
1	900.5
2	1259.0
3	1658.5
4	2058.0
5	1615.5
6	1173.0
7	1098.5
8	1024.0
9	1036.0
10	1048.0
11	1079.0
12	1110.0
13	1156.0
14	1202.0
15	1166.0
16	1130.0
17	1209.5
18	1289.0
19	1330.5
20	1372.0
21	1471.5
22	1571.0
23	1862.5
24	2154.0
25	2524.5
26	3621.5
27	4348.0
28	4976.5
29	5605.0
30	6475.5
31	7346.0
32	8777.0
33	10208.0
34	11274.0
35	12340.0
36	13612.5
37	14885.0
38	16678.0
39	18471.0
40	21470.0
41	24469.0
42	27470.0
43	30471.0
44	34584.0
45	38697.0
46	39733.0
47	40769.0
48	40838.0
49	40907.0
50	38757.5
51	36608.0
52	34138.0
53	31668.0
54	31124.0
55	30580.0
56	29725.5
57	28871.0
58	27964.0
59	27057.0
60	25356.5
61	23656.0
62	22009.0
63	20362.0
64	18237.0
65	16112.0
66	14033.0
67	11954.0
68	10164.0
69	8374.0
70	7221.0
71	6068.0
72	5090.0
73	4112.0
74	3307.5
75	1964.0
76	1425.0
77	1106.0
78	787.0
79	623.5
80	460.0
81	337.0
82	214.0
83	159.0
84	104.0
85	83.0
86	62.0
87	48.5
88	35.0
89	25.5
90	16.0
91	14.0
92	12.0
93	7.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	519389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.6238484020542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82191853838805	27.77464671837106
2	8.498395443396548	6.394846836461198
3	3.9317119019671742	4.437783976804955
4	2.628492667751598	3.9557603862958852
5	1.8167656758726975	3.4176858185544954
6	1.2806448848323613	2.8909673402279354
7	0.9998358148004529	2.6332369786097605
8	0.7284242335221054	2.1924898347534576
9	0.5558927779613251	1.88233430452323
>10	5.664802309324725	41.382227061975954
>50	0.05077482790487686	1.2325542247733603
>100	0.020309931161926247	1.2954045564445091
>500	0.002030993116192625	0.5100619622042234
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	749	0.1442079058278092	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG	737	0.14189749879184965	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC	592	0.11398008044067165	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	592	0.11398008044067165	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.061610854292254935	0.0
2	0.0	0.0	0.0	0.32981060438322723	0.0
3	0.0	0.0	0.0	0.44898910065480785	0.0
4	0.0	0.0	0.0	0.6465289022293502	0.0
5	0.0	0.0	0.0	1.2662955896255024	0.0
6	0.0	0.0	0.0	1.6219057392436111	0.0
7	0.0	0.0	0.0	2.0724351112557255	0.0
8	0.0	0.0	0.0	2.704909037349655	0.0
9	0.0	0.0	0.0	2.957321006028237	0.0
10	0.0	0.0	0.0	3.702234741205532	0.0
11	0.0	0.0	0.0	4.4925864814233645	0.0
12	0.0	0.0	0.0	5.324910616127796	0.0
13	0.0	0.0	0.0	5.577322584806378	0.0
14	0.0	0.0	0.0	5.715947006963952	0.0
15	0.0	0.0	0.0	5.936398344978427	0.0
16	0.0	0.0	0.0	6.350731340093841	0.0
17	0.0	0.0	0.0	6.841307767395921	0.0
18	0.0	0.0	0.0	7.370583512550324	0.0
19	0.0	0.0	0.0	7.716759500104931	0.0
20	0.0	0.0	0.0	8.054078927355027	0.0
21	0.0	0.0	0.0	8.467256718952461	0.0
22	0.0	0.0	0.0	8.872733153763365	0.0
23	0.0	0.0	0.0	9.279942393851236	0.0
24	0.0	0.0	0.0	9.624000508289548	0.0
25	0.0	0.0	0.0	9.948035095082876	0.0
26	0.0	0.0	0.0	10.239338915533445	0.0
27	0.0	0.0	0.0	10.59186852243694	0.0
28	0.0	0.0	0.0	10.911474829078013	0.0
29	0.0	0.0	0.0	11.260153757588244	0.0
30	0.0	0.0	0.0	11.681995575570527	0.0
31	0.0	0.0	0.0	12.025861156089174	0.0
32	0.0	0.0	0.0	12.353168819516778	0.0
33	0.0	0.0	0.0	12.674315397515157	0.0
34	0.0	0.0	0.0	13.033198623767543	0.0
35	0.0	0.0	0.0	13.427123023398646	0.0
36	0.0	0.0	0.0	13.788701724526318	0.0
37	0.0	0.0	0.0	14.137765720875874	0.0
38	1.9253391966329668E-4	0.0	0.0	14.524951433318765	0.0
39	1.9253391966329668E-4	0.0	0.0	15.169362462431819	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	30	2.163195E-6	45.000004	1
CGTTGCG	20	7.029712E-4	45.0	31
TTGTACG	25	3.887842E-5	45.0	1
ATGACGG	50	2.1827873E-11	45.0	2
GCGATAC	25	3.887842E-5	45.0	9
TAGGCGA	25	3.887842E-5	45.0	6
TATCGTT	20	7.029712E-4	45.0	40
TAGCTAC	20	7.029712E-4	45.0	29
TGTAACG	20	7.029712E-4	45.0	1
CTACGAA	50	1.0786607E-9	40.5	11
CGAATAT	50	1.0786607E-9	40.5	14
CGTTATT	135	0.0	40.0	1
AACGGGA	160	0.0	39.375	4
CACGACC	115	0.0	39.130436	27
CTAACGG	35	6.2416784E-6	38.571426	2
TATACGG	35	6.2416784E-6	38.571426	2
TAACTCG	35	6.2416784E-6	38.571426	38
GCGAGAC	120	0.0	37.500004	21
ACACGAC	120	0.0	37.500004	26
TTCACGG	60	1.546141E-10	37.500004	2
>>END_MODULE