FastQCFastQC Report
Sat 14 Jan 2017
SRR2936092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757071
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133521.7636390774445196No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42000.5547696319103492No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.3613927887873132No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.23828676570625476No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG207.0317596E-445.021
CGTTCGA253.88954E-545.014
TACGACG453.8380676E-1045.01
CATACGG302.1645155E-644.9999962
CGGTCTA302.1645155E-644.99999631
CGTTATT11400.044.2105261
CGTTTTA6600.043.2954521
CGTTTTT51600.042.906981
CACGGGT700.041.7857134
CGGTTTT16050.041.7757031
GTTATTT13150.039.182512
AGCACGG1150.039.1304322
CGACGGT356.2454637E-638.57142628
TAATGCG356.2454637E-638.5714261
CGGTTTA1600.037.968751
CACGACC1550.037.74193627
GTTTGCG601.546141E-1037.4999961
GCGTTAG301.13955386E-437.4999961
ACGTGCG301.13955386E-437.4999961
GTAACGG850.037.0588262
Produced by FastQC (version 0.11.5)