##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936092.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	757071
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.685627900157318	31.0	31.0	34.0	30.0	34.0
2	31.770612003365603	33.0	31.0	34.0	30.0	34.0
3	31.05921505380605	31.0	31.0	34.0	27.0	34.0
4	35.16875035498652	37.0	35.0	37.0	32.0	37.0
5	30.89107494541463	35.0	35.0	37.0	0.0	37.0
6	33.22924930422642	37.0	35.0	37.0	17.0	37.0
7	29.67388527628188	35.0	32.0	37.0	0.0	37.0
8	32.685716399122406	35.0	35.0	37.0	17.0	37.0
9	36.54102587471981	39.0	35.0	39.0	32.0	39.0
10	36.92134159147557	39.0	37.0	39.0	34.0	39.0
11	37.039296182260316	39.0	37.0	39.0	34.0	39.0
12	37.01326956124326	39.0	37.0	39.0	33.0	39.0
13	36.858745084675014	39.0	37.0	39.0	33.0	39.0
14	38.07076615007047	40.0	37.0	41.0	33.0	41.0
15	38.15650975932244	40.0	37.0	41.0	33.0	41.0
16	38.203378547058335	40.0	37.0	41.0	33.0	41.0
17	38.1495896686044	40.0	37.0	41.0	33.0	41.0
18	37.9733327521461	40.0	37.0	41.0	33.0	41.0
19	37.74190003315409	39.0	37.0	41.0	33.0	41.0
20	37.488172179359665	39.0	35.0	41.0	33.0	41.0
21	37.34537975962624	39.0	35.0	41.0	33.0	41.0
22	37.31029586392822	39.0	35.0	41.0	33.0	41.0
23	37.23113684185499	39.0	35.0	41.0	33.0	41.0
24	37.13643106128752	39.0	35.0	41.0	33.0	41.0
25	36.98675025195787	39.0	35.0	41.0	32.0	41.0
26	36.82912699073138	39.0	35.0	40.0	32.0	41.0
27	36.77347963401055	39.0	35.0	40.0	32.0	41.0
28	36.6241620667018	38.0	35.0	40.0	31.0	41.0
29	36.54449318491925	38.0	35.0	40.0	31.0	41.0
30	36.3264013546946	38.0	35.0	40.0	31.0	41.0
31	36.11284542665087	38.0	35.0	40.0	30.0	41.0
32	35.831940465293215	38.0	35.0	40.0	29.0	41.0
33	35.43677937736355	38.0	34.0	40.0	26.0	41.0
34	34.9737726052114	38.0	34.0	40.0	24.0	41.0
35	34.58247773326412	38.0	33.0	40.0	21.0	41.0
36	34.286193765181864	38.0	33.0	40.0	20.0	41.0
37	34.03491218128815	38.0	33.0	40.0	18.0	41.0
38	33.81745569438005	38.0	33.0	40.0	18.0	41.0
39	33.70558375634518	38.0	33.0	40.0	16.0	41.0
40	33.510587514248996	37.0	33.0	40.0	15.0	41.0
41	33.34782206688673	37.0	33.0	40.0	15.0	41.0
42	33.242372247781255	37.0	33.0	40.0	15.0	41.0
43	33.07415420746535	37.0	32.0	40.0	13.0	41.0
44	32.85924305646366	37.0	32.0	40.0	12.0	41.0
45	32.710024555160615	36.0	31.0	40.0	12.0	41.0
46	32.61292401901539	36.0	31.0	40.0	12.0	41.0
47	32.4369471291332	36.0	31.0	40.0	11.0	41.0
48	32.3256537365716	36.0	31.0	40.0	10.0	41.0
49	32.1880233161751	35.0	31.0	40.0	10.0	41.0
50	32.006663839983304	35.0	30.0	40.0	10.0	41.0
51	30.2922235827287	34.0	26.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	32.0
10	51.0
11	36.0
12	21.0
13	29.0
14	39.0
15	69.0
16	145.0
17	294.0
18	570.0
19	1016.0
20	1715.0
21	2918.0
22	4485.0
23	7102.0
24	10930.0
25	15917.0
26	19842.0
27	20829.0
28	19848.0
29	19429.0
30	20388.0
31	23529.0
32	28837.0
33	38686.0
34	53380.0
35	62092.0
36	69268.0
37	102690.0
38	122742.0
39	110117.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.37143411912489	18.51239844083316	21.855942177153796	21.26022526288816
2	40.28671022929157	21.64116707679993	22.384294207544603	15.687828486363895
3	31.320180009536752	21.911154964329633	31.749598122236883	15.019066903896727
4	25.154840166906407	23.56634978753644	34.06470463140181	17.21410541415534
5	19.811219819541364	37.06336657988484	28.226943047613762	14.898470552960028
6	23.618260374522336	30.95614546059749	32.36090142139905	13.064692743481126
7	63.10689486190859	18.3375139187738	15.126058190050868	3.4295330292667394
8	76.74683087847771	4.155356631016113	16.21935062893705	2.8784618615691264
9	71.54784689943216	5.851234560563012	17.954590784748063	4.646327755256772
10	38.538393360728385	23.54442317827522	24.758576144113302	13.158607316883092
11	28.95448379346191	24.211071352620824	30.884950024502327	15.949494829414942
12	25.4998540427516	21.965839399475083	34.407076747095054	18.12722981067826
13	23.016335323899607	22.1832562599809	36.60726668964998	18.193141726469513
14	19.989406541790665	23.324100381602253	37.702144184627336	18.98434889197975
15	20.186085585103644	22.65586715116548	38.85527249095527	18.302774772775603
16	24.63824396919179	22.510702430815606	35.23024921044394	17.62080438954867
17	23.891550462241984	22.616901188924157	34.870837741770586	18.620710607063273
18	24.88168216719436	21.819221711041635	35.3585066658213	17.940589455942703
19	23.575067596037886	24.736385358836884	33.20230202979641	18.486245015328816
20	24.67470025928876	24.772973736941452	33.24721195237963	17.30511405139016
21	23.602277725603017	24.601391415072037	34.77071503201153	17.025615827313423
22	23.138912994950275	21.914589252527175	35.40500164449569	19.541496108026855
23	21.681586007124828	24.046753871169283	35.03000379092582	19.24165633078007
24	21.873906146187082	23.30574014854617	35.67842382022294	19.141929885043808
25	22.71504257856925	24.077266200924353	33.735541316468336	19.472149904038062
26	21.82926039961906	23.91453377556398	33.54295700139089	20.713248823426074
27	21.494417300358883	23.128610130357654	34.95180769042798	20.425164878855483
28	20.164158975842426	24.978634764771073	34.37841364944635	20.47879260994015
29	21.976142264067704	24.787239241762002	33.530540728676705	19.706077765493593
30	22.504626382466107	23.31749598122237	35.20277490486362	18.975102731447908
31	23.19108775795137	24.031695838303143	33.428172522788486	19.349043880957005
32	23.454999597131575	24.11134490688456	33.28313989044621	19.150515605537656
33	23.177350605161205	24.244885882565836	32.39009287107814	20.187670641194817
34	21.404729543200045	25.639735242797574	33.98201753864565	18.97351767535674
35	21.086001180866788	26.620092435187715	32.979733736994284	19.31417264695121
36	22.324326252095247	28.329047077486784	29.786506153319834	19.56012051709813
37	21.696115687960575	27.809412855597426	30.255550668299275	20.23892078814272
38	22.323929988072454	27.145274353396182	29.97843002835929	20.55236563017207
39	22.656527591203467	25.963218773404346	29.656927817866492	21.7233258175257
40	23.6779641539565	24.749726247604254	30.47706225704062	21.095247341398625
41	21.55504569584623	25.664039436195544	30.526331083874563	22.25458378408366
42	22.232921350837636	26.010770456139515	29.925066473289824	21.831241719733026
43	21.59731385827749	25.334876121262074	31.446852408822952	21.620957611637483
44	21.848677336735918	25.442659935461798	30.799753259601808	21.90890946820047
45	22.118268960242833	25.20741119393029	29.968787603804664	22.705532242022215
46	21.673660726668963	25.677644500978115	30.080666146239913	22.56802862611301
47	20.63227887476868	26.64703838873765	31.211603667291442	21.509079069202226
48	21.386369310143962	26.375597533124367	30.75022025675267	21.487812899978998
49	22.136629193298912	25.57950311133302	30.892743216950592	21.39112447841748
50	20.776783155080565	25.760595769749468	31.563750295546917	21.898870779623046
51	20.698058702552334	25.19948591347443	30.728954087529438	23.373501296443795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	541.0
1	1007.0
2	1473.0
3	14720.0
4	27967.0
5	21242.0
6	14517.0
7	11465.0
8	8413.0
9	8073.5
10	7734.0
11	7409.0
12	7084.0
13	6780.0
14	6476.0
15	6177.0
16	5878.0
17	5500.5
18	5123.0
19	4941.0
20	4759.0
21	4700.0
22	4641.0
23	4838.5
24	5036.0
25	5585.0
26	7106.0
27	8078.0
28	9163.5
29	10249.0
30	11724.5
31	13200.0
32	14398.0
33	15596.0
34	17605.0
35	19614.0
36	21528.5
37	23443.0
38	25137.5
39	26832.0
40	28230.0
41	29628.0
42	31633.0
43	33638.0
44	36072.5
45	38507.0
46	43330.5
47	48154.0
48	49208.0
49	50262.0
50	50097.0
51	49932.0
52	48263.5
53	46595.0
54	44218.5
55	41842.0
56	39713.5
57	37585.0
58	35471.0
59	33357.0
60	31732.0
61	30107.0
62	27840.0
63	25573.0
64	22846.5
65	20120.0
66	17435.0
67	14750.0
68	12812.0
69	10874.0
70	9428.5
71	7983.0
72	6899.5
73	5816.0
74	4876.0
75	3121.5
76	2307.0
77	1834.5
78	1362.0
79	1138.0
80	914.0
81	690.0
82	466.0
83	362.5
84	259.0
85	223.0
86	187.0
87	123.5
88	60.0
89	47.5
90	35.0
91	27.0
92	19.0
93	15.0
94	11.0
95	7.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	757071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.393265102230544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47193476451115	33.80524277441474
2	9.392802671192623	8.527399634127718
3	4.332359444440007	5.899798223388524
4	2.6133793872320132	4.745192933493103
5	1.8564402250937628	4.213494164206286
6	1.3019098210259308	3.545876258701656
7	1.0156640100357786	3.227301396364405
8	0.7662854485538576	2.7827358808149523
9	0.6392609633741863	2.611632814195655
>10	3.566337040338252	25.550395687940668
>50	0.0274138047111971	0.8329461195806279
>100	0.01473856317388368	1.2229895918227356
>500	2.9477126347298723E-4	0.07894571763335766
>1k	8.843137904189617E-4	1.1694670713822812
>5k	0.0	0.0
>10k+	2.9477126347298723E-4	1.786581731933206
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13352	1.7636390774445196	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4200	0.5547696319103492	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2736	0.3613927887873132	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1804	0.23828676570625476	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3208800759770218E-4	0.0	0.0	0.04596662664400036	0.0
2	1.3208800759770218E-4	0.0	0.0	0.13671108786362177	0.0
3	1.3208800759770218E-4	0.0	0.0	0.25308062255719743	0.0
4	1.3208800759770218E-4	0.0	0.0	0.36786510115960064	0.0
5	1.3208800759770218E-4	0.0	0.0	0.7591097796639945	0.0
6	1.3208800759770218E-4	0.0	0.0	1.2514017839806306	0.0
7	1.3208800759770218E-4	0.0	0.0	1.6448919586141855	0.0
8	1.3208800759770218E-4	0.0	0.0	2.2538176736395927	0.0
9	1.3208800759770218E-4	0.0	0.0	2.6734612737774923	0.0
10	1.3208800759770218E-4	0.0	0.0	3.3397131841003023	0.0
11	1.3208800759770218E-4	0.0	0.0	3.838873764812019	0.0
12	1.3208800759770218E-4	0.0	0.0	4.303823551555931	0.0
13	1.3208800759770218E-4	0.0	0.0	4.548450541626875	0.0
14	1.3208800759770218E-4	0.0	0.0	4.719900775488693	0.0
15	1.3208800759770218E-4	0.0	0.0	4.8642729677929815	0.0
16	1.3208800759770218E-4	0.0	0.0	5.0501207944829485	0.0
17	1.3208800759770218E-4	0.0	0.0	5.260669078593685	0.0
18	1.3208800759770218E-4	0.0	0.0	5.512825085097699	0.0
19	1.3208800759770218E-4	0.0	0.0	5.670670254176953	0.0
20	1.3208800759770218E-4	0.0	0.0	5.83987499190961	0.0
21	1.3208800759770218E-4	0.0	0.0	6.026515346645163	0.0
22	1.3208800759770218E-4	0.0	0.0	6.230459230376015	0.0
23	1.3208800759770218E-4	0.0	0.0	6.4531596111857406	0.0
24	1.3208800759770218E-4	0.0	0.0	6.647857334384754	0.0
25	1.3208800759770218E-4	0.0	0.0	6.830535048892376	0.0
26	1.3208800759770218E-4	0.0	0.0	7.01770375565832	0.0
27	1.3208800759770218E-4	0.0	0.0	7.219270055252413	0.0
28	1.3208800759770218E-4	0.0	0.0	7.412514810367852	0.0
29	1.3208800759770218E-4	0.0	0.0	7.616590782106302	0.0
30	1.3208800759770218E-4	0.0	0.0	7.883936909484051	0.0
31	2.6417601519540437E-4	0.0	0.0	8.104655970179811	0.0
32	2.6417601519540437E-4	0.0	0.0	8.351264280364722	0.0
33	2.6417601519540437E-4	0.0	0.0	8.580304885539137	0.0
34	2.6417601519540437E-4	0.0	0.0	8.824799787602483	0.0
35	2.6417601519540437E-4	0.0	0.0	9.064935785415106	0.0
36	2.6417601519540437E-4	0.0	0.0	9.318941024025488	0.0
37	2.6417601519540437E-4	0.0	0.0	9.586287151403237	0.0
38	2.6417601519540437E-4	0.0	0.0	9.92297948276978	0.0
39	2.6417601519540437E-4	0.0	0.0	10.379739813042635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.0317596E-4	45.0	21
CGTTCGA	25	3.88954E-5	45.0	14
TACGACG	45	3.8380676E-10	45.0	1
CATACGG	30	2.1645155E-6	44.999996	2
CGGTCTA	30	2.1645155E-6	44.999996	31
CGTTATT	1140	0.0	44.210526	1
CGTTTTA	660	0.0	43.295452	1
CGTTTTT	5160	0.0	42.90698	1
CACGGGT	70	0.0	41.785713	4
CGGTTTT	1605	0.0	41.775703	1
GTTATTT	1315	0.0	39.18251	2
AGCACGG	115	0.0	39.130432	2
CGACGGT	35	6.2454637E-6	38.571426	28
TAATGCG	35	6.2454637E-6	38.571426	1
CGGTTTA	160	0.0	37.96875	1
CACGACC	155	0.0	37.741936	27
GTTTGCG	60	1.546141E-10	37.499996	1
GCGTTAG	30	1.13955386E-4	37.499996	1
ACGTGCG	30	1.13955386E-4	37.499996	1
GTAACGG	85	0.0	37.058826	2
>>END_MODULE