##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936092.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 757071 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.685627900157318 31.0 31.0 34.0 30.0 34.0 2 31.770612003365603 33.0 31.0 34.0 30.0 34.0 3 31.05921505380605 31.0 31.0 34.0 27.0 34.0 4 35.16875035498652 37.0 35.0 37.0 32.0 37.0 5 30.89107494541463 35.0 35.0 37.0 0.0 37.0 6 33.22924930422642 37.0 35.0 37.0 17.0 37.0 7 29.67388527628188 35.0 32.0 37.0 0.0 37.0 8 32.685716399122406 35.0 35.0 37.0 17.0 37.0 9 36.54102587471981 39.0 35.0 39.0 32.0 39.0 10 36.92134159147557 39.0 37.0 39.0 34.0 39.0 11 37.039296182260316 39.0 37.0 39.0 34.0 39.0 12 37.01326956124326 39.0 37.0 39.0 33.0 39.0 13 36.858745084675014 39.0 37.0 39.0 33.0 39.0 14 38.07076615007047 40.0 37.0 41.0 33.0 41.0 15 38.15650975932244 40.0 37.0 41.0 33.0 41.0 16 38.203378547058335 40.0 37.0 41.0 33.0 41.0 17 38.1495896686044 40.0 37.0 41.0 33.0 41.0 18 37.9733327521461 40.0 37.0 41.0 33.0 41.0 19 37.74190003315409 39.0 37.0 41.0 33.0 41.0 20 37.488172179359665 39.0 35.0 41.0 33.0 41.0 21 37.34537975962624 39.0 35.0 41.0 33.0 41.0 22 37.31029586392822 39.0 35.0 41.0 33.0 41.0 23 37.23113684185499 39.0 35.0 41.0 33.0 41.0 24 37.13643106128752 39.0 35.0 41.0 33.0 41.0 25 36.98675025195787 39.0 35.0 41.0 32.0 41.0 26 36.82912699073138 39.0 35.0 40.0 32.0 41.0 27 36.77347963401055 39.0 35.0 40.0 32.0 41.0 28 36.6241620667018 38.0 35.0 40.0 31.0 41.0 29 36.54449318491925 38.0 35.0 40.0 31.0 41.0 30 36.3264013546946 38.0 35.0 40.0 31.0 41.0 31 36.11284542665087 38.0 35.0 40.0 30.0 41.0 32 35.831940465293215 38.0 35.0 40.0 29.0 41.0 33 35.43677937736355 38.0 34.0 40.0 26.0 41.0 34 34.9737726052114 38.0 34.0 40.0 24.0 41.0 35 34.58247773326412 38.0 33.0 40.0 21.0 41.0 36 34.286193765181864 38.0 33.0 40.0 20.0 41.0 37 34.03491218128815 38.0 33.0 40.0 18.0 41.0 38 33.81745569438005 38.0 33.0 40.0 18.0 41.0 39 33.70558375634518 38.0 33.0 40.0 16.0 41.0 40 33.510587514248996 37.0 33.0 40.0 15.0 41.0 41 33.34782206688673 37.0 33.0 40.0 15.0 41.0 42 33.242372247781255 37.0 33.0 40.0 15.0 41.0 43 33.07415420746535 37.0 32.0 40.0 13.0 41.0 44 32.85924305646366 37.0 32.0 40.0 12.0 41.0 45 32.710024555160615 36.0 31.0 40.0 12.0 41.0 46 32.61292401901539 36.0 31.0 40.0 12.0 41.0 47 32.4369471291332 36.0 31.0 40.0 11.0 41.0 48 32.3256537365716 36.0 31.0 40.0 10.0 41.0 49 32.1880233161751 35.0 31.0 40.0 10.0 41.0 50 32.006663839983304 35.0 30.0 40.0 10.0 41.0 51 30.2922235827287 34.0 26.0 38.0 8.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 10.0 9 32.0 10 51.0 11 36.0 12 21.0 13 29.0 14 39.0 15 69.0 16 145.0 17 294.0 18 570.0 19 1016.0 20 1715.0 21 2918.0 22 4485.0 23 7102.0 24 10930.0 25 15917.0 26 19842.0 27 20829.0 28 19848.0 29 19429.0 30 20388.0 31 23529.0 32 28837.0 33 38686.0 34 53380.0 35 62092.0 36 69268.0 37 102690.0 38 122742.0 39 110117.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.37143411912489 18.51239844083316 21.855942177153796 21.26022526288816 2 40.28671022929157 21.64116707679993 22.384294207544603 15.687828486363895 3 31.320180009536752 21.911154964329633 31.749598122236883 15.019066903896727 4 25.154840166906407 23.56634978753644 34.06470463140181 17.21410541415534 5 19.811219819541364 37.06336657988484 28.226943047613762 14.898470552960028 6 23.618260374522336 30.95614546059749 32.36090142139905 13.064692743481126 7 63.10689486190859 18.3375139187738 15.126058190050868 3.4295330292667394 8 76.74683087847771 4.155356631016113 16.21935062893705 2.8784618615691264 9 71.54784689943216 5.851234560563012 17.954590784748063 4.646327755256772 10 38.538393360728385 23.54442317827522 24.758576144113302 13.158607316883092 11 28.95448379346191 24.211071352620824 30.884950024502327 15.949494829414942 12 25.4998540427516 21.965839399475083 34.407076747095054 18.12722981067826 13 23.016335323899607 22.1832562599809 36.60726668964998 18.193141726469513 14 19.989406541790665 23.324100381602253 37.702144184627336 18.98434889197975 15 20.186085585103644 22.65586715116548 38.85527249095527 18.302774772775603 16 24.63824396919179 22.510702430815606 35.23024921044394 17.62080438954867 17 23.891550462241984 22.616901188924157 34.870837741770586 18.620710607063273 18 24.88168216719436 21.819221711041635 35.3585066658213 17.940589455942703 19 23.575067596037886 24.736385358836884 33.20230202979641 18.486245015328816 20 24.67470025928876 24.772973736941452 33.24721195237963 17.30511405139016 21 23.602277725603017 24.601391415072037 34.77071503201153 17.025615827313423 22 23.138912994950275 21.914589252527175 35.40500164449569 19.541496108026855 23 21.681586007124828 24.046753871169283 35.03000379092582 19.24165633078007 24 21.873906146187082 23.30574014854617 35.67842382022294 19.141929885043808 25 22.71504257856925 24.077266200924353 33.735541316468336 19.472149904038062 26 21.82926039961906 23.91453377556398 33.54295700139089 20.713248823426074 27 21.494417300358883 23.128610130357654 34.95180769042798 20.425164878855483 28 20.164158975842426 24.978634764771073 34.37841364944635 20.47879260994015 29 21.976142264067704 24.787239241762002 33.530540728676705 19.706077765493593 30 22.504626382466107 23.31749598122237 35.20277490486362 18.975102731447908 31 23.19108775795137 24.031695838303143 33.428172522788486 19.349043880957005 32 23.454999597131575 24.11134490688456 33.28313989044621 19.150515605537656 33 23.177350605161205 24.244885882565836 32.39009287107814 20.187670641194817 34 21.404729543200045 25.639735242797574 33.98201753864565 18.97351767535674 35 21.086001180866788 26.620092435187715 32.979733736994284 19.31417264695121 36 22.324326252095247 28.329047077486784 29.786506153319834 19.56012051709813 37 21.696115687960575 27.809412855597426 30.255550668299275 20.23892078814272 38 22.323929988072454 27.145274353396182 29.97843002835929 20.55236563017207 39 22.656527591203467 25.963218773404346 29.656927817866492 21.7233258175257 40 23.6779641539565 24.749726247604254 30.47706225704062 21.095247341398625 41 21.55504569584623 25.664039436195544 30.526331083874563 22.25458378408366 42 22.232921350837636 26.010770456139515 29.925066473289824 21.831241719733026 43 21.59731385827749 25.334876121262074 31.446852408822952 21.620957611637483 44 21.848677336735918 25.442659935461798 30.799753259601808 21.90890946820047 45 22.118268960242833 25.20741119393029 29.968787603804664 22.705532242022215 46 21.673660726668963 25.677644500978115 30.080666146239913 22.56802862611301 47 20.63227887476868 26.64703838873765 31.211603667291442 21.509079069202226 48 21.386369310143962 26.375597533124367 30.75022025675267 21.487812899978998 49 22.136629193298912 25.57950311133302 30.892743216950592 21.39112447841748 50 20.776783155080565 25.760595769749468 31.563750295546917 21.898870779623046 51 20.698058702552334 25.19948591347443 30.728954087529438 23.373501296443795 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 541.0 1 1007.0 2 1473.0 3 14720.0 4 27967.0 5 21242.0 6 14517.0 7 11465.0 8 8413.0 9 8073.5 10 7734.0 11 7409.0 12 7084.0 13 6780.0 14 6476.0 15 6177.0 16 5878.0 17 5500.5 18 5123.0 19 4941.0 20 4759.0 21 4700.0 22 4641.0 23 4838.5 24 5036.0 25 5585.0 26 7106.0 27 8078.0 28 9163.5 29 10249.0 30 11724.5 31 13200.0 32 14398.0 33 15596.0 34 17605.0 35 19614.0 36 21528.5 37 23443.0 38 25137.5 39 26832.0 40 28230.0 41 29628.0 42 31633.0 43 33638.0 44 36072.5 45 38507.0 46 43330.5 47 48154.0 48 49208.0 49 50262.0 50 50097.0 51 49932.0 52 48263.5 53 46595.0 54 44218.5 55 41842.0 56 39713.5 57 37585.0 58 35471.0 59 33357.0 60 31732.0 61 30107.0 62 27840.0 63 25573.0 64 22846.5 65 20120.0 66 17435.0 67 14750.0 68 12812.0 69 10874.0 70 9428.5 71 7983.0 72 6899.5 73 5816.0 74 4876.0 75 3121.5 76 2307.0 77 1834.5 78 1362.0 79 1138.0 80 914.0 81 690.0 82 466.0 83 362.5 84 259.0 85 223.0 86 187.0 87 123.5 88 60.0 89 47.5 90 35.0 91 27.0 92 19.0 93 15.0 94 11.0 95 7.0 96 3.0 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 757071.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.393265102230544 #Duplication Level Percentage of deduplicated Percentage of total 1 74.47193476451115 33.80524277441474 2 9.392802671192623 8.527399634127718 3 4.332359444440007 5.899798223388524 4 2.6133793872320132 4.745192933493103 5 1.8564402250937628 4.213494164206286 6 1.3019098210259308 3.545876258701656 7 1.0156640100357786 3.227301396364405 8 0.7662854485538576 2.7827358808149523 9 0.6392609633741863 2.611632814195655 >10 3.566337040338252 25.550395687940668 >50 0.0274138047111971 0.8329461195806279 >100 0.01473856317388368 1.2229895918227356 >500 2.9477126347298723E-4 0.07894571763335766 >1k 8.843137904189617E-4 1.1694670713822812 >5k 0.0 0.0 >10k+ 2.9477126347298723E-4 1.786581731933206 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13352 1.7636390774445196 No Hit CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4200 0.5547696319103492 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2736 0.3613927887873132 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1804 0.23828676570625476 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.3208800759770218E-4 0.0 0.0 0.04596662664400036 0.0 2 1.3208800759770218E-4 0.0 0.0 0.13671108786362177 0.0 3 1.3208800759770218E-4 0.0 0.0 0.25308062255719743 0.0 4 1.3208800759770218E-4 0.0 0.0 0.36786510115960064 0.0 5 1.3208800759770218E-4 0.0 0.0 0.7591097796639945 0.0 6 1.3208800759770218E-4 0.0 0.0 1.2514017839806306 0.0 7 1.3208800759770218E-4 0.0 0.0 1.6448919586141855 0.0 8 1.3208800759770218E-4 0.0 0.0 2.2538176736395927 0.0 9 1.3208800759770218E-4 0.0 0.0 2.6734612737774923 0.0 10 1.3208800759770218E-4 0.0 0.0 3.3397131841003023 0.0 11 1.3208800759770218E-4 0.0 0.0 3.838873764812019 0.0 12 1.3208800759770218E-4 0.0 0.0 4.303823551555931 0.0 13 1.3208800759770218E-4 0.0 0.0 4.548450541626875 0.0 14 1.3208800759770218E-4 0.0 0.0 4.719900775488693 0.0 15 1.3208800759770218E-4 0.0 0.0 4.8642729677929815 0.0 16 1.3208800759770218E-4 0.0 0.0 5.0501207944829485 0.0 17 1.3208800759770218E-4 0.0 0.0 5.260669078593685 0.0 18 1.3208800759770218E-4 0.0 0.0 5.512825085097699 0.0 19 1.3208800759770218E-4 0.0 0.0 5.670670254176953 0.0 20 1.3208800759770218E-4 0.0 0.0 5.83987499190961 0.0 21 1.3208800759770218E-4 0.0 0.0 6.026515346645163 0.0 22 1.3208800759770218E-4 0.0 0.0 6.230459230376015 0.0 23 1.3208800759770218E-4 0.0 0.0 6.4531596111857406 0.0 24 1.3208800759770218E-4 0.0 0.0 6.647857334384754 0.0 25 1.3208800759770218E-4 0.0 0.0 6.830535048892376 0.0 26 1.3208800759770218E-4 0.0 0.0 7.01770375565832 0.0 27 1.3208800759770218E-4 0.0 0.0 7.219270055252413 0.0 28 1.3208800759770218E-4 0.0 0.0 7.412514810367852 0.0 29 1.3208800759770218E-4 0.0 0.0 7.616590782106302 0.0 30 1.3208800759770218E-4 0.0 0.0 7.883936909484051 0.0 31 2.6417601519540437E-4 0.0 0.0 8.104655970179811 0.0 32 2.6417601519540437E-4 0.0 0.0 8.351264280364722 0.0 33 2.6417601519540437E-4 0.0 0.0 8.580304885539137 0.0 34 2.6417601519540437E-4 0.0 0.0 8.824799787602483 0.0 35 2.6417601519540437E-4 0.0 0.0 9.064935785415106 0.0 36 2.6417601519540437E-4 0.0 0.0 9.318941024025488 0.0 37 2.6417601519540437E-4 0.0 0.0 9.586287151403237 0.0 38 2.6417601519540437E-4 0.0 0.0 9.92297948276978 0.0 39 2.6417601519540437E-4 0.0 0.0 10.379739813042635 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATCG 20 7.0317596E-4 45.0 21 CGTTCGA 25 3.88954E-5 45.0 14 TACGACG 45 3.8380676E-10 45.0 1 CATACGG 30 2.1645155E-6 44.999996 2 CGGTCTA 30 2.1645155E-6 44.999996 31 CGTTATT 1140 0.0 44.210526 1 CGTTTTA 660 0.0 43.295452 1 CGTTTTT 5160 0.0 42.90698 1 CACGGGT 70 0.0 41.785713 4 CGGTTTT 1605 0.0 41.775703 1 GTTATTT 1315 0.0 39.18251 2 AGCACGG 115 0.0 39.130432 2 CGACGGT 35 6.2454637E-6 38.571426 28 TAATGCG 35 6.2454637E-6 38.571426 1 CGGTTTA 160 0.0 37.96875 1 CACGACC 155 0.0 37.741936 27 GTTTGCG 60 1.546141E-10 37.499996 1 GCGTTAG 30 1.13955386E-4 37.499996 1 ACGTGCG 30 1.13955386E-4 37.499996 1 GTAACGG 85 0.0 37.058826 2 >>END_MODULE