##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936091.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 625808 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69508219773477 31.0 31.0 34.0 30.0 34.0 2 31.791573453838875 31.0 31.0 34.0 30.0 34.0 3 31.43601232326848 31.0 31.0 34.0 28.0 34.0 4 35.35879534937233 37.0 35.0 37.0 33.0 37.0 5 31.149541073300437 37.0 35.0 37.0 0.0 37.0 6 33.344412343722034 37.0 35.0 37.0 17.0 37.0 7 29.554758008846164 35.0 32.0 37.0 0.0 37.0 8 32.56609215606065 35.0 33.0 37.0 17.0 37.0 9 36.38721780482193 39.0 35.0 39.0 32.0 39.0 10 36.70596093370491 38.0 35.0 39.0 33.0 39.0 11 36.877655766624905 39.0 37.0 39.0 33.0 39.0 12 36.948508168639584 39.0 37.0 39.0 33.0 39.0 13 36.81940627157211 39.0 37.0 39.0 33.0 39.0 14 38.0792815048705 40.0 37.0 41.0 33.0 41.0 15 38.14092341421011 40.0 37.0 41.0 33.0 41.0 16 38.165339848643676 40.0 37.0 41.0 33.0 41.0 17 38.0985493953417 40.0 37.0 41.0 33.0 41.0 18 37.97133306061923 40.0 37.0 41.0 33.0 41.0 19 37.80207188147163 39.0 37.0 41.0 33.0 41.0 20 37.66777829621865 39.0 36.0 41.0 33.0 41.0 21 37.569254467823995 39.0 36.0 41.0 33.0 41.0 22 37.528553486053234 39.0 35.0 41.0 33.0 41.0 23 37.47418697108378 39.0 35.0 41.0 33.0 41.0 24 37.40292070411372 39.0 35.0 41.0 33.0 41.0 25 37.272067471173266 39.0 35.0 41.0 32.0 41.0 26 37.09244848260169 39.0 35.0 41.0 32.0 41.0 27 37.06174417712781 39.0 35.0 41.0 32.0 41.0 28 36.91663257740393 39.0 35.0 41.0 31.0 41.0 29 36.877494375271645 39.0 35.0 41.0 31.0 41.0 30 36.70468578222074 39.0 35.0 40.0 31.0 41.0 31 36.54258494618158 39.0 35.0 40.0 30.0 41.0 32 36.380683851916245 39.0 35.0 40.0 30.0 41.0 33 36.11573198169407 39.0 35.0 40.0 30.0 41.0 34 35.77705622171656 38.0 35.0 40.0 27.0 41.0 35 35.55413002070923 38.0 35.0 40.0 26.0 41.0 36 35.337609937872315 38.0 34.0 40.0 25.0 41.0 37 35.18556649962928 38.0 34.0 40.0 24.0 41.0 38 35.09664146191803 38.0 34.0 40.0 24.0 41.0 39 35.0100621915987 38.0 34.0 40.0 24.0 41.0 40 34.889547592871935 38.0 34.0 40.0 23.0 41.0 41 34.76901381893488 38.0 34.0 40.0 23.0 41.0 42 34.77522658706824 38.0 34.0 40.0 23.0 41.0 43 34.64377572674047 38.0 34.0 40.0 23.0 41.0 44 34.51832990310127 38.0 33.0 40.0 22.0 41.0 45 34.3818743128883 38.0 33.0 40.0 22.0 41.0 46 34.23841018331501 38.0 33.0 40.0 22.0 41.0 47 34.06007114003017 37.0 33.0 40.0 20.0 41.0 48 33.939994375271645 37.0 33.0 40.0 20.0 41.0 49 33.77488462915143 37.0 33.0 40.0 20.0 41.0 50 33.5823846930688 37.0 32.0 40.0 19.0 41.0 51 31.555846202030015 35.0 29.0 39.0 12.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 16.0 9 29.0 10 25.0 11 19.0 12 18.0 13 19.0 14 31.0 15 31.0 16 93.0 17 149.0 18 303.0 19 574.0 20 985.0 21 1814.0 22 2738.0 23 4072.0 24 6022.0 25 8572.0 26 10818.0 27 11758.0 28 12210.0 29 12999.0 30 15438.0 31 18816.0 32 23304.0 33 32214.0 34 44727.0 35 54147.0 36 62872.0 37 93082.0 38 110351.0 39 97553.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.365907754455044 20.3265218725232 23.949997443305296 16.35757292971646 2 33.59576739191573 25.405236110756015 24.901087873597014 16.097908623731243 3 30.99832536496817 24.15149694474983 29.533019712116175 15.317157978165827 4 26.469620075166823 24.573191777669827 30.802099046352875 18.15508910081047 5 21.06189118707335 37.86736507043694 24.872325058164805 16.198418684324906 6 24.220847288625265 33.90273054994503 28.014502850714596 13.861919310715107 7 65.60718303377394 19.038746708255566 11.26479687060568 4.089273387364814 8 78.75530514151305 5.294115767136246 12.069516529031269 3.8810625623194337 9 72.79277350241607 7.321095288011659 13.809826656098995 6.076304553473269 10 37.49584537110424 25.852497890726866 21.69131746478153 14.960339273387365 11 27.805493058573877 25.356179531102192 28.256589880602355 18.581737529721575 12 24.84675811111395 22.876345460588553 32.48887837803288 19.78801805026462 13 22.078177332344744 24.644779229412215 33.853194590034 19.423848848209037 14 19.560152634673894 27.399457980722524 32.86119704446092 20.179192340142663 15 19.57629176999974 25.99215733899215 35.90430291718866 18.527247973819446 16 22.058203154961266 25.117767749852995 32.92463503183085 19.899394063354894 17 21.186050673689056 25.351385728530158 31.606339324521258 21.85622427325953 18 22.8234538388771 25.47762252959374 31.659710326489915 20.039213305039244 19 22.362449824866413 27.521859739728477 29.877694117045483 20.237996318359624 20 23.545240712806486 26.965778641372435 30.576151151790963 18.912829494030117 21 22.57689259325544 27.789353923248022 30.99528929000588 18.638464193490655 22 21.84727584179173 24.74976350573978 30.42402781683839 22.978932835630097 23 20.614948993940633 26.767954388566462 31.348272952726713 21.268823664766188 24 21.06332932784496 24.932567177153373 32.400991997545574 21.60311149745609 25 20.851283460741953 27.416555876562782 29.904858998287015 21.827301664408253 26 19.924481630148545 27.448035180119142 30.45886278219518 22.168620407537137 27 21.49684887377598 27.002531127758033 30.36666197939304 21.133958019072942 28 19.466194104262012 26.665846393782118 31.725065834888653 22.142893667067217 29 21.286241147444585 25.959239894664176 30.16052846879554 22.5939904890957 30 21.28208651854882 26.154347659346 31.13367039091862 21.429895431186562 31 22.297413903305806 25.902992611152303 28.957284023214786 22.842309462327105 32 22.979092629049166 26.36367703832485 29.571689719530593 21.08554061309539 33 22.96471122133306 26.0356211489786 29.407741671567 21.59192595812134 34 21.284803006672973 25.693503438754377 31.045784010431316 21.975909544141334 35 22.211764630685447 26.105291079692172 29.680988418172987 22.00195587144939 36 22.08968245851763 27.712653082095468 29.15926290491652 21.038401554470383 37 21.963126070615907 27.998363715388745 30.08702349602434 19.951486717971008 38 22.296614936210467 27.21889141717588 28.269373354127787 22.215120292485874 39 23.246107432311508 25.48449364661366 29.844616879298442 21.424782041776393 40 23.46726152430138 24.806969549766062 30.51974407486002 21.206024851072534 41 20.313738398997774 26.6172691943855 30.54930585738757 22.519686549229156 42 21.583616700329813 25.897559634903995 30.259600388617596 22.259223276148596 43 21.870445887556567 26.142522946334978 29.709751233605196 22.277279932503262 44 21.542869378467515 25.751828036714137 28.969747909902075 23.73555467491627 45 22.37619205890625 25.622075780431057 28.43859458492061 23.56313757574208 46 22.7136757599775 26.537052898013446 28.3676463068545 22.38162503515455 47 20.977200674967403 26.019002633395544 30.73562498402066 22.268171707616393 48 21.52049829979802 25.424571114463223 30.178105744893003 22.876824840845757 49 21.255241224145426 25.259824099404288 30.570079001866386 22.914855674583897 50 20.211310817375296 24.62832050724823 30.69375910822489 24.466609567151586 51 20.30686728197786 24.54299082146601 29.60844220591619 25.54169969063994 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 639.0 1 963.0 2 1287.0 3 6155.0 4 11023.0 5 8711.5 6 6400.0 7 5102.0 8 3804.0 9 3635.5 10 3467.0 11 3273.0 12 3079.0 13 3032.0 14 2985.0 15 2852.5 16 2720.0 17 2694.0 18 2668.0 19 2797.0 20 2926.0 21 3114.5 22 3303.0 23 3544.5 24 3786.0 25 4439.0 26 5892.0 27 6692.0 28 7823.0 29 8954.0 30 10379.0 31 11804.0 32 13850.0 33 15896.0 34 17941.5 35 19987.0 36 20576.5 37 21166.0 38 22380.0 39 23594.0 40 26213.0 41 28832.0 42 31148.0 43 33464.0 44 35964.5 45 38465.0 46 41811.5 47 45158.0 48 50475.5 49 55793.0 50 54829.0 51 53865.0 52 50909.0 53 47953.0 54 41903.5 55 35854.0 56 32506.0 57 29158.0 58 26684.0 59 24210.0 60 22536.5 61 20863.0 62 18741.0 63 16619.0 64 13845.0 65 11071.0 66 9386.5 67 7702.0 68 6513.0 69 5324.0 70 4563.5 71 3803.0 72 3176.0 73 2549.0 74 2120.5 75 1274.5 76 857.0 77 715.5 78 574.0 79 444.0 80 314.0 81 245.0 82 176.0 83 155.5 84 135.0 85 92.0 86 49.0 87 33.0 88 17.0 89 16.5 90 16.0 91 13.0 92 10.0 93 7.5 94 5.0 95 6.0 96 7.0 97 4.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 625808.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.71572014999454 #Duplication Level Percentage of deduplicated Percentage of total 1 76.51236648805882 33.447932014058 2 8.671422352466198 7.5815494572563935 3 3.7724543963265162 4.947466820052798 4 2.205649357432914 3.8568620023421025 5 1.5434361297154364 3.3736210958015342 6 1.088384925713318 2.85477184967736 7 0.8335804500304766 2.550839877322718 8 0.6737729342955289 2.35635752322432 9 0.5591859731525216 2.200069576272419 >10 4.0634629899368155 31.395774971802382 >50 0.053361223855718655 1.5828027363292523 >100 0.019540729652698062 1.637835303494185 >500 0.0018789163127594286 0.6222788495121337 >1k 0.0011273497876556572 0.7610923732285415 >5k 3.757832625518857E-4 0.8307455496258871 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5057 0.8080753202260118 No Hit CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1752 0.2799580702068366 No Hit GAATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC 1733 0.27692199524454786 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG 1148 0.1834428450898678 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 958 0.1530820954669803 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGC 943 0.15068519418096285 No Hit GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC 712 0.11377291437629433 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 671 0.10722138419451334 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.12927287602587376 0.0 2 0.0 0.0 0.0 0.5111791475979853 0.0 3 0.0 0.0 0.0 0.7201889397387058 0.0 4 0.0 0.0 0.0 1.0250747833201237 0.0 5 0.0 0.0 0.0 2.022505305141513 0.0 6 0.0 0.0 0.0 2.7511632960908137 0.0 7 0.0 0.0 0.0 3.3626927108633957 0.0 8 0.0 0.0 0.0 4.259133791833917 0.0 9 0.0 0.0 0.0 4.676354409020019 0.0 10 0.0 0.0 0.0 5.730990974867691 0.0 11 0.0 0.0 0.0 6.6752102881394935 0.0 12 0.0 0.0 0.0 7.6379656380231635 0.0 13 0.0 0.0 0.0 8.013799759670698 0.0 14 0.0 0.0 0.0 8.189093140388106 0.0 15 0.0 0.0 0.0 8.432618311047477 0.0 16 0.0 0.0 0.0 8.897137780277657 0.0 17 0.0 0.0 0.0 9.444270447165904 0.0 18 0.0 0.0 0.0 10.04141845422238 0.0 19 0.0 0.0 0.0 10.392963976171606 0.0 20 0.0 0.0 0.0 10.737318794262777 0.0 21 0.0 0.0 0.0 11.166683711298035 0.0 22 0.0 0.0 0.0 11.625929997698975 0.0 23 0.0 0.0 0.0 12.128640094086364 0.0 24 0.0 0.0 0.0 12.526046327308055 0.0 25 0.0 0.0 0.0 12.887658834658554 0.0 26 0.0 0.0 0.0 13.233292000102267 0.0 27 0.0 0.0 0.0 13.578285991869711 0.0 28 0.0 0.0 0.0 13.93606345716258 0.0 29 0.0 0.0 0.0 14.31285633932452 0.0 30 0.0 0.0 0.0 14.71921100401401 0.0 31 0.0 0.0 0.0 15.114380129368753 0.0 32 0.0 0.0 0.0 15.51130698233324 0.0 33 0.0 0.0 0.0 15.883306061923145 0.0 34 1.597934190678291E-4 0.0 0.0 16.259939150666018 0.0 35 1.597934190678291E-4 0.0 0.0 16.64615984455296 0.0 36 1.597934190678291E-4 0.0 0.0 17.05267430266152 0.0 37 1.597934190678291E-4 0.0 0.0 17.438735203129394 0.0 38 1.597934190678291E-4 0.0 0.0 17.839497098151508 0.0 39 1.597934190678291E-4 0.0 0.0 18.25607854166134 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCGTC 30 2.1639098E-6 45.000004 8 CGCATAG 20 7.03082E-4 45.000004 2 GTTGTCG 20 7.03082E-4 45.000004 37 TCTCGTA 40 6.8066583E-9 45.000004 28 GTCAACG 30 2.1639098E-6 45.000004 1 AGTTACG 20 7.03082E-4 45.000004 1 TTCGAAG 40 6.8066583E-9 45.000004 1 TCGAATT 20 7.03082E-4 45.000004 12 CGTATGG 50 2.1827873E-11 45.0 2 CGATAGC 35 1.2107375E-7 45.0 10 GCGTATG 25 3.8887625E-5 45.0 1 CGGTTTT 650 0.0 42.923077 1 CGTTATT 495 0.0 42.727272 1 GCTACGA 75 0.0 42.0 10 CGTTTTT 2170 0.0 41.785713 1 CGTTTTA 355 0.0 41.19718 1 TGCGATG 50 1.0804797E-9 40.5 1 TCGTACG 45 1.925946E-8 40.000004 30 CACGTGA 90 0.0 40.000004 43 AGGCGAT 120 0.0 39.375004 7 >>END_MODULE