##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936087.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	612412
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.804153086484263	31.0	31.0	34.0	30.0	34.0
2	31.92363637551191	33.0	31.0	34.0	30.0	34.0
3	31.752617518925167	33.0	31.0	34.0	30.0	34.0
4	35.551898068620474	37.0	35.0	37.0	33.0	37.0
5	31.044992586690007	37.0	35.0	37.0	0.0	37.0
6	33.296040900570205	37.0	35.0	37.0	17.0	37.0
7	29.647988608975655	35.0	32.0	37.0	0.0	37.0
8	32.62028013820761	35.0	33.0	37.0	17.0	37.0
9	36.451844509905094	39.0	35.0	39.0	32.0	39.0
10	36.73941235638753	38.0	35.0	39.0	33.0	39.0
11	36.87474608596827	39.0	37.0	39.0	33.0	39.0
12	36.95332553901622	39.0	37.0	39.0	33.0	39.0
13	36.78989961006643	39.0	37.0	39.0	33.0	39.0
14	38.07044603959426	40.0	37.0	41.0	33.0	41.0
15	38.14853562634305	40.0	37.0	41.0	33.0	41.0
16	38.178582392245744	40.0	37.0	41.0	33.0	41.0
17	38.11876645134321	40.0	37.0	41.0	33.0	41.0
18	38.01626029535672	40.0	37.0	41.0	33.0	41.0
19	37.88751363461199	40.0	37.0	41.0	33.0	41.0
20	37.829606539388514	40.0	36.0	41.0	33.0	41.0
21	37.76926644154589	40.0	36.0	41.0	33.0	41.0
22	37.74863817168834	39.0	36.0	41.0	33.0	41.0
23	37.722908760768895	39.0	36.0	41.0	33.0	41.0
24	37.640407111552356	39.0	36.0	41.0	33.0	41.0
25	37.53418613613058	39.0	36.0	41.0	33.0	41.0
26	37.3553522791846	39.0	36.0	41.0	32.0	41.0
27	37.336730828265935	39.0	36.0	41.0	32.0	41.0
28	37.217092414910226	39.0	35.0	41.0	32.0	41.0
29	37.2215077431533	39.0	36.0	41.0	32.0	41.0
30	37.09547494170591	39.0	35.0	41.0	31.0	41.0
31	36.9784524143877	39.0	35.0	41.0	31.0	41.0
32	36.85859356119737	39.0	35.0	41.0	31.0	41.0
33	36.69168958152355	39.0	35.0	41.0	30.0	41.0
34	36.51814464772082	39.0	35.0	41.0	30.0	41.0
35	36.390281379202236	39.0	35.0	41.0	30.0	41.0
36	36.263810963860934	39.0	35.0	40.0	30.0	41.0
37	36.1219750756027	39.0	35.0	40.0	29.0	41.0
38	36.01074603371586	39.0	35.0	40.0	29.0	41.0
39	35.96549381788731	39.0	35.0	40.0	28.0	41.0
40	35.8022671012325	39.0	35.0	40.0	27.0	41.0
41	35.66185672390482	38.0	35.0	40.0	27.0	41.0
42	35.62818658027602	38.0	35.0	40.0	27.0	41.0
43	35.48420181185215	38.0	35.0	40.0	26.0	41.0
44	35.33547513765243	38.0	34.0	40.0	25.0	41.0
45	35.20778985388921	38.0	34.0	40.0	25.0	41.0
46	35.06028457966206	38.0	34.0	40.0	24.0	41.0
47	34.87686230838063	38.0	34.0	40.0	23.0	41.0
48	34.8051769070495	38.0	34.0	40.0	23.0	41.0
49	34.62802819017263	38.0	34.0	40.0	23.0	41.0
50	34.45910106268329	38.0	33.0	40.0	23.0	41.0
51	32.415667883712274	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	12.0
9	25.0
10	24.0
11	21.0
12	9.0
13	8.0
14	11.0
15	23.0
16	67.0
17	119.0
18	277.0
19	481.0
20	892.0
21	1358.0
22	2130.0
23	3061.0
24	4565.0
25	6014.0
26	7719.0
27	8768.0
28	9922.0
29	11331.0
30	13837.0
31	16926.0
32	21609.0
33	29398.0
34	41333.0
35	50678.0
36	60387.0
37	93324.0
38	117201.0
39	110867.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80745641822825	20.640843092558605	24.67358575599433	11.878114733218814
2	32.32366446117973	26.132407594887102	24.738084818716814	16.805843125216356
3	31.229466437626957	25.629151616885366	26.994735570171713	16.146646375315964
4	26.823119076699996	25.6626258139945	29.013474589002175	18.500780520303326
5	22.09215364819762	38.594279667935965	22.695179062461218	16.618387621405198
6	24.557977309393024	35.76236259250309	25.900864124151713	13.778795973952176
7	69.23672299040516	18.58569067882406	8.577885475790808	3.5997008549799805
8	82.45772453838266	5.648158429292698	8.310091898917722	3.584025133406922
9	76.66293279687531	7.644853464661046	9.446908290497246	6.245305447966402
10	37.64753140042977	31.713291052428755	17.47418404603437	13.164993501107098
11	28.12371410096471	26.92958335238369	27.451290960987045	17.495411585664552
12	26.86622731102591	23.757535776568712	30.64783185175993	18.728405060645446
13	23.155326806137044	25.764518004219383	31.86204711860643	19.218108071037147
14	19.616532661019054	29.830244998465083	29.912052670424487	20.64116967009138
15	18.91782002965324	27.618498657766338	35.43202941810415	18.031651894476266
16	21.761820473798686	26.5344245377295	32.55831041847645	19.145444569995362
17	21.49974200374911	25.232686492100086	30.505444047471308	22.76212745667949
18	22.82091141257846	27.24097502988184	29.18884672410077	20.749266833438927
19	23.32155477031802	29.43916840297055	27.418469918943455	19.820806907767974
20	25.758639608629487	27.513014114680967	28.83891889773551	17.889427378954036
21	23.865143073617105	27.921072741879648	29.134634853660607	19.079149330842636
22	22.63019013343958	25.630947793315613	28.572921497292675	23.16594057595214
23	21.825666381455623	28.78503360482812	28.632032030724414	20.75726798299184
24	22.38052160963534	26.545691462610137	29.32111062487345	21.752676302881067
25	21.37156032213608	28.63627753865045	28.296147038268355	21.696015100945115
26	21.020979340705274	28.697510826045214	28.77654258897605	21.504967244273463
27	22.117463406987454	28.634971228519362	28.99257362690476	20.25499173758842
28	19.918453590066818	29.01739351939544	30.33856292822479	20.725589962312952
29	21.982097019653434	26.704408143537357	29.013474589002175	22.30002024780703
30	21.798887023768312	26.08195136607382	30.72114850786725	21.398013102290612
31	21.45385786039464	27.71304285350385	28.326845326348927	22.506253959752584
32	22.114034342893348	28.060194770840546	28.613090533823637	21.212680352442472
33	21.6517638452545	27.428920399992162	29.03992736915671	21.879388385596624
34	20.39558336544679	28.305291209185974	29.381364179669895	21.91776124569734
35	20.39346061148377	26.263855051827857	29.070788946003674	24.2718953906847
36	21.421036818351045	27.68920269361149	29.412552334049625	21.47720815398784
37	21.19504516567278	28.289125621313755	29.80754785993743	20.708281353076032
38	21.144752225625886	27.604292535090757	28.56034826228095	22.69060697700241
39	22.60879930504301	26.60823106013599	28.253365381475216	22.529604253345788
40	21.955480950732515	25.485130924932886	30.888682782179316	21.67070534215528
41	20.00303717105478	28.44914861237206	28.794504353278512	22.753309863294643
42	21.39131826286879	26.6536253371913	29.661567702788318	22.293488697151588
43	22.668236416007524	26.610843680398162	28.20960399208376	22.51131591151055
44	21.56636382043461	27.400344865874608	28.192132094080456	22.841159219610326
45	21.00563019666499	26.20768371619106	28.08844372742533	24.69824235971862
46	21.69797456614175	28.279491584096984	27.497828259407065	22.524705590354205
47	20.552177292410992	27.067072493680726	30.269655068809886	22.111095145098396
48	21.592326734289987	25.71814399456575	28.856880662037977	23.83264860910629
49	20.535195260706846	25.97679340052122	29.715616284462094	23.772395054309843
50	19.52051886638407	26.51613619589427	29.446679686224307	24.516665251497358
51	19.91257519447692	25.678954690633105	27.998145039613853	26.41032507527612
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	268.0
1	585.0
2	902.0
3	3155.5
4	5409.0
5	4302.5
6	3196.0
7	2615.5
8	2035.0
9	1961.0
10	1887.0
11	1870.5
12	1854.0
13	1845.0
14	1836.0
15	1758.5
16	1681.0
17	1781.0
18	1881.0
19	1939.0
20	1997.0
21	2255.0
22	2513.0
23	2913.0
24	3313.0
25	3614.5
26	5628.0
27	7340.0
28	8500.5
29	9661.0
30	11688.0
31	13715.0
32	15241.0
33	16767.0
34	19011.0
35	21255.0
36	22959.5
37	24664.0
38	26270.5
39	27877.0
40	30967.5
41	34058.0
42	37018.0
43	39978.0
44	43501.5
45	47025.0
46	53488.5
47	59952.0
48	55673.0
49	51394.0
50	49042.0
51	46690.0
52	42287.0
53	37884.0
54	33300.5
55	28717.0
56	26676.5
57	24636.0
58	23908.0
59	23180.0
60	21938.0
61	20696.0
62	18819.0
63	16942.0
64	13422.0
65	9902.0
66	7876.5
67	5851.0
68	4646.5
69	3442.0
70	2957.5
71	2473.0
72	2183.5
73	1894.0
74	1617.0
75	1181.5
76	1023.0
77	838.0
78	653.0
79	516.5
80	380.0
81	277.0
82	174.0
83	123.5
84	73.0
85	56.5
86	40.0
87	34.0
88	28.0
89	20.0
90	12.0
91	7.0
92	2.0
93	3.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	612412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.30559496987047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50623101958217	29.67817334157166
2	8.598969578493191	6.759752308209823
3	3.6315303637343757	4.2821838479318926
4	2.221187263766107	3.492203477673018
5	1.6195210350124476	3.182811892369234
6	1.1591388168372174	2.7336384509075122
7	0.9036938570126157	2.486415730434854
8	0.7071483959710037	2.223591074850388
9	0.5850076906970032	2.06946678103161
>10	4.966079837533222	37.66037929716639
>50	0.0775108759015982	1.8909187307476645
>100	0.020983607276658357	1.6815273376623203
>500	0.001284710649591328	0.34573144660244304
>1k	0.0017129475327884375	1.5132062828412671
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC	2534	0.41377373402219425	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG	2393	0.39075001796176434	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2296	0.3749110076223196	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC	1767	0.28853125020411097	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	768	0.12540577258446928	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	675	0.1102199173105687	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08229753825855797	0.0
2	0.0	0.0	0.0	0.4082219159650693	0.0
3	0.0	0.0	0.0	0.5550185169461082	0.0
4	0.0	0.0	0.0	0.8536736706661529	0.0
5	0.0	0.0	0.0	1.9281137534862152	0.0
6	0.0	0.0	0.0	2.41504085484935	0.0
7	0.0	0.0	0.0	2.9031109775771866	0.0
8	0.0	0.0	0.0	3.672037778488991	0.0
9	0.0	0.0	0.0	3.978694081761951	0.0
10	0.0	0.0	0.0	5.2319353637747135	0.0
11	0.0	0.0	0.0	6.0325401853654075	0.0
12	0.0	0.0	0.0	7.08803877128469	0.0
13	0.0	0.0	0.0	7.405145555606357	0.0
14	0.0	0.0	0.0	7.55912686230838	0.0
15	0.0	0.0	0.0	7.794425974670647	0.0
16	0.0	0.0	0.0	8.232366446117974	0.0
17	0.0	0.0	0.0	8.78493563156829	0.0
18	1.632887663860277E-4	0.0	0.0	9.389430644729366	0.0
19	1.632887663860277E-4	0.0	0.0	9.746706465581994	0.0
20	1.632887663860277E-4	0.0	0.0	10.082754746804438	0.0
21	1.632887663860277E-4	0.0	0.0	10.50942829337113	0.0
22	1.632887663860277E-4	0.0	0.0	10.998314859930897	0.0
23	1.632887663860277E-4	0.0	0.0	11.50826567735446	0.0
24	1.632887663860277E-4	0.0	0.0	11.898035962717909	0.0
25	1.632887663860277E-4	0.0	0.0	12.244044858689902	0.0
26	1.632887663860277E-4	0.0	0.0	12.57176541282666	0.0
27	1.632887663860277E-4	0.0	0.0	12.891974683709659	0.0
28	1.632887663860277E-4	0.0	0.0	13.241086066242987	0.0
29	1.632887663860277E-4	0.0	0.0	13.601301084890563	0.0
30	1.632887663860277E-4	0.0	0.0	14.0297708078875	0.0
31	3.265775327720554E-4	0.0	0.0	14.418887938185405	0.0
32	3.265775327720554E-4	0.0	0.0	14.841969131891602	0.0
33	3.265775327720554E-4	0.0	0.0	15.219329471009713	0.0
34	4.898662991580831E-4	0.0	0.0	15.575298981731253	0.0
35	4.898662991580831E-4	0.0	0.0	15.95282260961575	0.0
36	4.898662991580831E-4	0.0	0.0	16.337857520754003	0.0
37	4.898662991580831E-4	0.0	0.0	16.763877912255147	0.0
38	4.898662991580831E-4	0.0	0.0	17.155934240348	0.0
39	4.898662991580831E-4	0.0	0.0	17.57852556775504	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGCA	55	1.8189894E-12	45.000004	22
GCGTCAT	55	1.8189894E-12	45.000004	37
ATTACGA	20	7.0307025E-4	45.0	10
CTCGCGA	20	7.0307025E-4	45.0	32
TAGTGCG	20	7.0307025E-4	45.0	1
TATACGG	20	7.0307025E-4	45.0	2
TCGACCG	20	7.0307025E-4	45.0	24
AGAATCG	20	7.0307025E-4	45.0	32
TAAGACG	20	7.0307025E-4	45.0	1
ATCGACC	25	3.8886636E-5	45.0	23
ATCCGGT	30	2.1638352E-6	44.999996	11
GTATCCG	30	2.1638352E-6	44.999996	9
TCGTACC	30	2.1638352E-6	44.999996	45
CGGGTAT	50	1.0804797E-9	40.5	6
GCGAGAC	145	0.0	40.34483	21
ACGGGAC	180	0.0	40.0	5
CGGTTTT	355	0.0	39.929577	1
TTACGGG	160	0.0	39.375	3
CGATGCA	40	3.4546974E-7	39.375	10
TTCGCGG	40	3.4546974E-7	39.375	2
>>END_MODULE