##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936086.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397475
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.846979055286496	31.0	31.0	34.0	30.0	34.0
2	31.99075413548022	33.0	31.0	34.0	30.0	34.0
3	31.927635700358515	33.0	31.0	34.0	30.0	34.0
4	35.66683690798163	37.0	35.0	37.0	33.0	37.0
5	31.078699289263476	37.0	35.0	37.0	0.0	37.0
6	33.32535127995472	37.0	35.0	37.0	17.0	37.0
7	29.589277313038554	35.0	32.0	37.0	0.0	37.0
8	32.585095917982265	35.0	33.0	37.0	17.0	37.0
9	36.393871312661176	39.0	35.0	39.0	32.0	39.0
10	36.750557896723066	38.0	35.0	39.0	33.0	39.0
11	36.88846342537266	39.0	37.0	39.0	33.0	39.0
12	36.88443549908799	39.0	35.0	39.0	33.0	39.0
13	36.61567645763885	39.0	35.0	39.0	33.0	39.0
14	37.81367884772627	40.0	37.0	41.0	33.0	41.0
15	37.93915088999308	40.0	37.0	41.0	33.0	41.0
16	38.00458896785961	40.0	37.0	41.0	33.0	41.0
17	37.9691401974967	40.0	36.0	41.0	33.0	41.0
18	37.87537580979936	40.0	36.0	41.0	33.0	41.0
19	37.7951720233977	40.0	36.0	41.0	33.0	41.0
20	37.75884521039059	39.0	36.0	41.0	33.0	41.0
21	37.69367130008177	39.0	36.0	41.0	33.0	41.0
22	37.67725265739983	39.0	36.0	41.0	33.0	41.0
23	37.63651550411976	39.0	36.0	41.0	33.0	41.0
24	37.57570161645386	39.0	36.0	41.0	33.0	41.0
25	37.451014529215676	39.0	35.0	41.0	33.0	41.0
26	37.25339706899805	39.0	35.0	41.0	32.0	41.0
27	37.20141392540411	39.0	35.0	41.0	32.0	41.0
28	37.105246870872385	39.0	35.0	41.0	32.0	41.0
29	37.0210755393421	39.0	35.0	41.0	31.0	41.0
30	36.779155921756086	39.0	35.0	41.0	31.0	41.0
31	36.66016730611988	39.0	35.0	40.0	30.0	41.0
32	36.52022391345368	39.0	35.0	40.0	30.0	41.0
33	36.296066419271654	39.0	35.0	40.0	30.0	41.0
34	36.13964903453047	39.0	35.0	40.0	30.0	41.0
35	35.90558903075665	39.0	35.0	40.0	28.0	41.0
36	35.73961381218945	38.0	35.0	40.0	27.0	41.0
37	35.60032958047676	38.0	35.0	40.0	27.0	41.0
38	35.48187433171898	38.0	34.0	40.0	26.0	41.0
39	35.389835838732	38.0	34.0	40.0	25.0	41.0
40	35.23680231461098	38.0	34.0	40.0	24.0	41.0
41	35.03992703943644	38.0	34.0	40.0	24.0	41.0
42	35.03092018365935	38.0	34.0	40.0	24.0	41.0
43	34.79975344361281	38.0	34.0	40.0	23.0	41.0
44	34.59277438832631	38.0	33.0	40.0	23.0	41.0
45	34.46534750613246	38.0	33.0	40.0	23.0	41.0
46	34.21495439964777	38.0	33.0	40.0	22.0	41.0
47	34.054657525630546	37.0	33.0	40.0	21.0	41.0
48	33.908313730423295	37.0	33.0	40.0	20.0	41.0
49	33.76529593056167	37.0	33.0	40.0	20.0	41.0
50	33.59627901125857	37.0	32.0	40.0	19.0	41.0
51	31.307244480784956	35.0	28.0	39.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	6.0
9	18.0
10	18.0
11	8.0
12	12.0
13	15.0
14	12.0
15	26.0
16	52.0
17	113.0
18	196.0
19	336.0
20	611.0
21	1012.0
22	1726.0
23	2512.0
24	3510.0
25	4405.0
26	5404.0
27	6113.0
28	7191.0
29	8239.0
30	10021.0
31	12214.0
32	15628.0
33	21440.0
34	30012.0
35	35779.0
36	38799.0
37	58168.0
38	69898.0
39	63969.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.028052078747095	21.347002956160765	25.33693943015284	11.288005534939305
2	31.900119504371343	26.66708598025033	24.354990879929556	17.07780363544877
3	31.794452481288133	26.427070884961317	24.964588967859612	16.813887665890938
4	28.62544814139254	25.863765016667717	25.718347065853198	19.792439776086546
5	22.56619913202088	40.053588275992205	20.390967985407887	16.989244606579028
6	24.729857223724764	36.02415246241902	25.168878545820494	14.077111768035724
7	67.64853135417322	20.163029121328385	7.818353355556954	4.370086168941443
8	80.23624127303603	6.92345430530222	8.25259450279892	4.587709918862822
9	74.23787659601233	8.027423108371595	9.98905591546638	7.745644380149695
10	40.44053085099692	26.375495314170706	18.5365117302975	14.647462104534876
11	32.198503050506325	24.99603748663438	24.0	18.8054594628593
12	29.238568463425374	22.59991194414743	28.3487011761746	19.812818416252593
13	23.24146172715265	26.353858733253666	30.839675451286247	19.565004088307443
14	19.616831247248253	30.720422668092333	28.506950122649222	21.155795962010192
15	19.11164224165042	26.187558965972702	35.29303729794327	19.40776149443361
16	20.58670356626203	25.781747279703126	32.03094534247437	21.600603811560475
17	20.94899050254733	24.497641361091894	28.803824139883012	25.749543996477765
18	21.34775772061136	26.457009874834895	30.70734008428203	21.487892320271715
19	23.986162651739104	27.70061010126423	26.595383357443865	21.717843889552803
20	25.220957292911507	28.05534939304359	27.421850430844707	19.3018428832002
21	23.274671362978804	27.88074721680609	28.574878923202718	20.26970249701239
22	21.56890370463551	26.489464746210455	26.433109000566073	25.508522548587965
23	21.318573495188375	28.662431599471667	27.71696333102711	22.30203157431285
24	22.18932008302409	25.926410466067047	29.10723944902195	22.77703000188691
25	20.684319768538902	28.08377885401598	28.283539845273285	22.948361532171834
26	20.071199446506068	29.813447386628088	27.207497326875906	22.907855839989935
27	22.012453613434808	28.68004276998553	27.826404176363294	21.48109944021637
28	18.846468331341594	27.797974715390904	30.64695892823448	22.708598025033023
29	22.078118120636518	25.902258003648026	29.187496068935154	22.8321278067803
30	21.64035473929178	26.780552235989685	29.88590477388515	21.693188250833387
31	23.601735958236368	26.911378074092713	27.50235863890811	21.984527328762816
32	24.41310774262532	26.441914585823007	26.34379520724574	22.80118246430593
33	22.66029310019498	25.781244103402727	28.812629725139942	22.745833071262346
34	22.9262217749544	25.134410969243348	28.67853324108435	23.260834014717908
35	21.761871815837473	24.329328888609346	28.436505440593745	25.47229385495943
36	22.777533178187305	25.480596263915974	31.121454179508145	20.62041637838858
37	21.423737341971194	25.992326561418956	31.101075539342098	21.482860557267752
38	20.7248254607208	26.428580413862505	28.360274231083714	24.48631989433298
39	22.726712371847285	25.479086735014782	29.436568337631297	22.357632555506633
40	23.479464117240077	24.400779923265613	29.9193659978615	22.20038996163281
41	21.02144788980439	24.981445373922888	29.175671425875844	24.82143531039688
42	21.629033272532862	25.33593307755205	29.36763318447701	23.667400465438078
43	22.62381281841625	23.798477891691302	29.8119378577269	23.765771432165543
44	21.647902383797724	25.17038807472168	29.137430027045724	24.044279514434873
45	21.127618089188	24.643562488206804	28.234480155984652	25.994339266620543
46	23.444744952512735	26.55965784011573	27.729794326687212	22.265802880684323
47	21.40386187810554	24.207560223913454	31.171268633247372	23.217309264733633
48	21.088118749606892	23.873451160450344	30.478646455751935	24.55978363419083
49	20.418391093779483	23.025599094282658	32.50040883074407	24.055600981193788
50	20.338889238316877	23.510912636014844	31.302849235800995	24.847348889867288
51	20.580665450657275	24.535379583621612	28.292345430530226	26.59160953519089
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	519.5
2	816.0
3	1094.5
4	1373.0
5	1094.0
6	815.0
7	751.5
8	688.0
9	713.0
10	738.0
11	790.0
12	842.0
13	882.0
14	922.0
15	924.0
16	926.0
17	987.5
18	1049.0
19	1231.5
20	1414.0
21	1593.5
22	1773.0
23	1702.0
24	1631.0
25	2160.5
26	3296.5
27	3903.0
28	5060.0
29	6217.0
30	6901.0
31	7585.0
32	8259.0
33	8933.0
34	9630.0
35	10327.0
36	11353.5
37	12380.0
38	14022.5
39	15665.0
40	17928.0
41	20191.0
42	23368.0
43	26545.0
44	29822.0
45	33099.0
46	36661.0
47	40223.0
48	39595.5
49	38968.0
50	36807.0
51	34646.0
52	30762.0
53	26878.0
54	23399.5
55	19921.0
56	18175.0
57	16429.0
58	15524.5
59	14620.0
60	13634.0
61	12648.0
62	11426.5
63	10205.0
64	8525.5
65	6846.0
66	5807.0
67	4768.0
68	4214.0
69	3660.0
70	3126.5
71	2593.0
72	2183.0
73	1773.0
74	1450.0
75	865.5
76	604.0
77	484.0
78	364.0
79	292.5
80	221.0
81	166.0
82	111.0
83	81.5
84	52.0
85	43.0
86	34.0
87	25.5
88	17.0
89	13.0
90	9.0
91	8.0
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	397475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.94347206818276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.51655915937674	28.653604531490817
2	8.68864405078559	6.593546457027307
3	3.588071351353763	4.0843165529621475
4	2.1738482206041847	3.299333969558546
5	1.5612051947706618	2.961877285024121
6	1.1996583748962117	2.731152242354155
7	0.9390520867072363	2.4941627635781325
8	0.6708579780908778	2.0363744762727025
9	0.5288254456314329	1.805892617273451
>10	4.984423944836721	40.07525919775843
>50	0.12172965112074716	2.8064507608672424
>100	0.025139819253197783	1.7267955978538247
>500	0.0013231483817472518	0.4586212468787524
>1k	6.615741908736259E-4	0.2726123011003422
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG	1086	0.2732247311151645	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC	960	0.24152462419020065	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	867	0.21812692622177496	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	438	0.11019560978677903	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	422	0.10617019938360904	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5158815019812566E-4	0.0	0.0	0.10742814013459966	0.0
2	2.5158815019812566E-4	0.0	0.0	0.6775268884835525	0.0
3	5.031763003962513E-4	0.0	0.0	0.9376690357884143	0.0
4	5.031763003962513E-4	0.0	0.0	1.309516321781244	0.0
5	5.031763003962513E-4	0.0	0.0	2.703063085728662	0.0
6	5.031763003962513E-4	0.0	0.0	3.3355556953267502	0.0
7	5.031763003962513E-4	0.0	0.0	4.075476445059437	0.0
8	5.031763003962513E-4	0.0	0.0	5.253915340587458	0.0
9	5.031763003962513E-4	0.0	0.0	5.700987483489528	0.0
10	5.031763003962513E-4	0.0	0.0	7.250770488709982	0.0
11	5.031763003962513E-4	0.0	0.0	8.561544751242216	0.0
12	5.031763003962513E-4	0.0	0.0	9.937731932825963	0.0
13	5.031763003962513E-4	0.0	0.0	10.393609660984968	0.0
14	5.031763003962513E-4	0.0	0.0	10.572488835775834	0.0
15	5.031763003962513E-4	0.0	0.0	10.933266243159947	0.0
16	5.031763003962513E-4	0.0	0.0	11.621863010252216	2.5158815019812566E-4
17	5.031763003962513E-4	0.0	0.0	12.447323731052267	2.5158815019812566E-4
18	5.031763003962513E-4	0.0	0.0	13.395811057299202	2.5158815019812566E-4
19	5.031763003962513E-4	0.0	0.0	13.877350776778414	2.5158815019812566E-4
20	5.031763003962513E-4	0.0	0.0	14.358890496257626	2.5158815019812566E-4
21	5.031763003962513E-4	0.0	0.0	14.929240832756777	2.5158815019812566E-4
22	5.031763003962513E-4	0.0	0.0	15.5302849235801	2.5158815019812566E-4
23	5.031763003962513E-4	0.0	0.0	16.1343480722058	2.5158815019812566E-4
24	5.031763003962513E-4	0.0	0.0	16.615636203534812	2.5158815019812566E-4
25	5.031763003962513E-4	0.0	0.0	17.023460595005975	2.5158815019812566E-4
26	5.031763003962513E-4	0.0	0.0	17.406126171457323	2.5158815019812566E-4
27	5.031763003962513E-4	0.0	0.0	17.785772690106295	2.5158815019812566E-4
28	5.031763003962513E-4	0.0	0.0	18.200641549783004	2.5158815019812566E-4
29	5.031763003962513E-4	0.0	0.0	18.623058053965657	2.5158815019812566E-4
30	5.031763003962513E-4	0.0	0.0	19.085477074029814	2.5158815019812566E-4
31	5.031763003962513E-4	0.0	0.0	19.554940562299517	2.5158815019812566E-4
32	5.031763003962513E-4	0.0	0.0	19.977357066482167	2.5158815019812566E-4
33	5.031763003962513E-4	0.0	0.0	20.397006101012643	2.5158815019812566E-4
34	5.031763003962513E-4	0.0	0.0	20.796276495377068	2.5158815019812566E-4
35	5.031763003962513E-4	0.0	0.0	21.225234291464872	2.5158815019812566E-4
36	5.031763003962513E-4	0.0	0.0	21.664507201710798	2.5158815019812566E-4
37	5.031763003962513E-4	0.0	0.0	22.11711428391723	2.5158815019812566E-4
38	5.031763003962513E-4	0.0	0.0	22.521164853135417	2.5158815019812566E-4
39	5.031763003962513E-4	0.0	0.0	22.94509088621926	2.5158815019812566E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGTA	30	2.1619053E-6	45.000004	35
GCGATAA	35	1.209246E-7	45.000004	9
TTCGCTA	30	2.1619053E-6	45.000004	14
CTTAACG	20	7.0277124E-4	45.0	1
GTACCAC	20	7.0277124E-4	45.0	36
CGAACCA	20	7.0277124E-4	45.0	14
ACACGAC	40	6.7957444E-9	45.0	26
CGAACAC	20	7.0277124E-4	45.0	38
CTCGTTG	20	7.0277124E-4	45.0	18
ATTAGCG	20	7.0277124E-4	45.0	1
ACGTTTC	20	7.0277124E-4	45.0	28
TCGATGG	45	3.8380676E-10	45.0	2
TAGCCGC	20	7.0277124E-4	45.0	13
CCGTCAG	25	3.886185E-5	45.0	30
GTTAACG	20	7.0277124E-4	45.0	1
CTACGTC	20	7.0277124E-4	45.0	37
GAAACCG	20	7.0277124E-4	45.0	38
TCCCGCT	20	7.0277124E-4	45.0	21
CGGTCTA	20	7.0277124E-4	45.0	31
CGCAATC	20	7.0277124E-4	45.0	20
>>END_MODULE