##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936084.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415939
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89675889974251	31.0	31.0	34.0	30.0	34.0
2	32.046961213062495	33.0	31.0	34.0	30.0	34.0
3	31.97296718990044	33.0	31.0	34.0	30.0	34.0
4	35.713046865045115	37.0	35.0	37.0	35.0	37.0
5	31.24246343814838	37.0	35.0	37.0	0.0	37.0
6	33.4261995148327	37.0	35.0	37.0	17.0	37.0
7	29.480902247685357	35.0	32.0	37.0	0.0	37.0
8	32.5492800627015	35.0	33.0	37.0	17.0	37.0
9	36.39541134637531	39.0	35.0	39.0	32.0	39.0
10	36.80389672524096	38.0	35.0	39.0	33.0	39.0
11	36.93743794162125	39.0	37.0	39.0	33.0	39.0
12	36.85284140222485	39.0	35.0	39.0	33.0	39.0
13	36.612397490978246	39.0	35.0	39.0	33.0	39.0
14	37.827099646823214	40.0	36.0	41.0	33.0	41.0
15	37.908616407694396	40.0	36.0	41.0	33.0	41.0
16	37.972277184875665	40.0	36.0	41.0	33.0	41.0
17	37.92671761965096	40.0	36.0	41.0	33.0	41.0
18	37.85187251015173	40.0	36.0	41.0	33.0	41.0
19	37.78087892695804	40.0	36.0	41.0	33.0	41.0
20	37.717557141792426	40.0	36.0	41.0	33.0	41.0
21	37.593909203032176	39.0	36.0	41.0	33.0	41.0
22	37.585814266034205	39.0	35.0	41.0	33.0	41.0
23	37.52559630138073	39.0	35.0	41.0	33.0	41.0
24	37.49374307290252	39.0	35.0	41.0	33.0	41.0
25	37.34927717766307	39.0	35.0	41.0	32.0	41.0
26	37.19394430433309	39.0	35.0	41.0	32.0	41.0
27	37.129891642764925	39.0	35.0	41.0	32.0	41.0
28	37.04379007498696	39.0	35.0	41.0	32.0	41.0
29	37.031369984541	39.0	35.0	41.0	32.0	41.0
30	36.91618482517869	39.0	35.0	41.0	31.0	41.0
31	36.78544450027528	39.0	35.0	41.0	31.0	41.0
32	36.63197007253468	39.0	35.0	40.0	31.0	41.0
33	36.50344401462714	39.0	35.0	40.0	30.0	41.0
34	36.35892763121515	39.0	35.0	40.0	30.0	41.0
35	36.262670728159655	39.0	35.0	40.0	30.0	41.0
36	36.14918533727301	39.0	35.0	40.0	30.0	41.0
37	36.06612748503987	38.0	35.0	40.0	29.0	41.0
38	35.94101058087845	38.0	35.0	40.0	29.0	41.0
39	35.94519388660356	38.0	35.0	40.0	29.0	41.0
40	35.82180079290473	38.0	35.0	40.0	29.0	41.0
41	35.7344201914223	38.0	35.0	40.0	28.0	41.0
42	35.67693339648362	38.0	35.0	40.0	28.0	41.0
43	35.504535520833585	38.0	34.0	40.0	27.0	41.0
44	35.34008352186258	38.0	34.0	40.0	27.0	41.0
45	35.20878782706118	38.0	34.0	40.0	26.0	41.0
46	35.089664590240396	38.0	34.0	40.0	26.0	41.0
47	34.959157472610165	38.0	34.0	40.0	26.0	41.0
48	34.88051853757402	37.0	34.0	40.0	25.0	41.0
49	34.721711597133236	37.0	34.0	40.0	24.0	41.0
50	34.52351186111425	37.0	34.0	40.0	24.0	41.0
51	32.59275999605711	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	37.0
10	27.0
11	22.0
12	19.0
13	18.0
14	21.0
15	27.0
16	67.0
17	114.0
18	253.0
19	380.0
20	635.0
21	960.0
22	1507.0
23	2039.0
24	2853.0
25	3654.0
26	4487.0
27	5508.0
28	6251.0
29	7319.0
30	9158.0
31	11807.0
32	15188.0
33	22191.0
34	32571.0
35	38613.0
36	40461.0
37	62761.0
38	76388.0
39	70582.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16137702884318	21.30264293562277	24.476906469458264	11.059073566075794
2	32.918288499034716	24.732472790481296	24.32231649352429	18.026922216959697
3	33.142119397315476	24.44877734475488	25.207782872007673	17.201320385921974
4	29.18360624995492	25.679486655495154	25.651117110922517	19.48578998362741
5	21.801754584205856	39.22714628827785	21.30528755418463	17.665811573331666
6	25.902115454429612	36.04975729614198	24.201144879417413	13.846982370010988
7	68.81345581924273	20.005577740966825	6.877210360173006	4.303756079617444
8	82.49959729671899	5.870331947713487	7.732143415260411	3.897927340307113
9	75.76279214019364	8.69454415190689	9.730513368546829	5.8121503393526455
10	42.1989762921967	27.2138462611104	16.50794948297707	14.079227963715834
11	33.96435535018356	25.840808387768398	23.170464899901187	17.024371362146855
12	30.71580207674683	23.680876282339476	27.339826272602473	18.26349536831122
13	25.752574295750097	26.290153123414733	27.94015468614388	20.01711789469129
14	20.961006301404773	29.660599270566117	28.441910953288822	20.936483474740285
15	20.366688384594855	26.639002353710524	34.45385020399626	18.540459057698364
16	23.351741481322982	24.324720692216886	32.96565121327887	19.35788661318126
17	22.714147988046324	24.96063124640873	28.641219024905094	23.684001740639854
18	24.341309663195805	25.06232885110557	29.00617638644128	21.59018509925734
19	26.931352914730283	27.27635542711792	25.8521081216236	19.940183536528192
20	28.900391643967023	25.897307057044422	25.681410014449234	19.520891284539317
21	25.023861672024022	28.17528531827984	26.900579171465044	19.900273838231087
22	24.51585448827833	25.163305196194635	26.820759774870833	23.500080540656203
23	23.013951565013137	27.714640848778306	27.54851071911987	21.722896867088686
24	23.887396950033537	24.889466965107864	29.82071890349306	21.40241718136554
25	23.02092374122167	26.434164625101275	27.228271453265982	23.31664018041107
26	22.463149644539225	27.83076364563073	27.583371600162526	22.122715109667524
27	22.424201625719157	28.11373783174937	27.769456578969514	21.692603963561965
28	20.44338232288869	28.583037416544254	29.12782884028668	21.845751420280376
29	22.25133973972145	26.738776599453285	28.736425293131923	22.273458367693337
30	23.576293639211517	25.66265726464698	28.79845361940092	21.962595476740578
31	24.90028585922455	26.15864345492969	26.429115807846827	22.51195487799894
32	25.274379175792603	27.166723966735507	27.203989046470756	20.354907811001134
33	25.03419972640219	26.960924558649225	26.487778255946186	21.5170974590024
34	21.30480671444611	27.059015865307174	29.361036113468565	22.275141306778156
35	23.05939092030322	26.09637470879143	27.925248654249778	22.91898571665557
36	22.838445060453576	29.14297529205004	26.974628491197027	21.04395115629936
37	23.254852274011334	28.508026417335238	26.75199969226257	21.485121616390863
38	21.974376050334303	29.11556742695443	25.588607944914997	23.321448577796264
39	22.498491365320394	27.126814268438398	27.662950576887475	22.71174378935373
40	23.82031980651009	25.441951824666596	28.50514137890412	22.232586989919195
41	20.50997862667362	26.24783922642503	27.902889606408632	25.339292540492714
42	22.906483883454065	26.54331524574517	26.334390379358513	24.215810491442255
43	23.451275307196486	25.96582671978343	26.665448539329084	23.917449433690997
44	21.499065968807926	26.958039520218108	27.102531861643175	24.44036264933079
45	22.270092489523705	26.651263767042764	26.673382395014656	24.40526134841888
46	22.529024688716373	27.830042386022953	26.472872224052086	23.16806070120859
47	21.19950281171037	27.642274468131145	27.98487278182618	23.1733499383323
48	21.902009669687143	25.678524976018117	28.537838481123433	23.881626873171307
49	21.714722591533857	25.778058801891625	28.62799593209581	23.879222674478708
50	19.625714347536537	26.849369739312735	28.312324643757858	25.21259126939287
51	20.30586215767216	25.896585797436643	27.32612234005467	26.471429704836524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	349.0
1	686.5
2	1024.0
3	1326.0
4	1628.0
5	1388.0
6	1148.0
7	1040.5
8	933.0
9	941.0
10	949.0
11	923.0
12	897.0
13	981.0
14	1065.0
15	1107.5
16	1150.0
17	1218.5
18	1287.0
19	1405.5
20	1524.0
21	1797.0
22	2070.0
23	2212.0
24	2354.0
25	2874.5
26	3624.0
27	3853.0
28	4759.0
29	5665.0
30	6676.0
31	7687.0
32	8814.0
33	9941.0
34	10823.5
35	11706.0
36	12562.5
37	13419.0
38	15025.5
39	16632.0
40	18821.0
41	21010.0
42	22951.0
43	24892.0
44	26893.0
45	28894.0
46	30785.0
47	32676.0
48	32425.5
49	32175.0
50	31591.5
51	31008.0
52	29558.5
53	28109.0
54	25872.5
55	23636.0
56	22232.0
57	20828.0
58	20331.0
59	19834.0
60	19111.0
61	18388.0
62	16780.5
63	15173.0
64	12509.0
65	9845.0
66	8179.0
67	6513.0
68	5706.0
69	4899.0
70	3993.0
71	3087.0
72	2802.0
73	2517.0
74	2064.5
75	1289.5
76	967.0
77	748.5
78	530.0
79	396.5
80	263.0
81	213.5
82	164.0
83	135.0
84	106.0
85	79.0
86	52.0
87	45.0
88	38.0
89	29.0
90	20.0
91	17.0
92	14.0
93	11.5
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	415939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.860519950818926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.93123384183176	24.694894985391848
2	8.416728713029494	5.699896210163314
3	3.9035053187299282	3.965241591689476
4	2.4595130808664907	3.331215669759198
5	1.8411233423284201	3.1170696832414952
6	1.3397561240837983	2.7218903381262773
7	1.1231482255985408	2.6621268030424394
8	0.8841222956206586	2.3949472503861715
9	0.6975922030922702	2.1258751239307387
>10	6.198510013061423	44.18011835714398
>50	0.1827259664291858	3.7265218082551685
>100	0.022040875328034084	1.3802021788698806
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	485	0.11660363659094242	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.808397385193502E-4	0.0	0.0	0.054094470583426896	0.0
2	4.808397385193502E-4	0.0	0.0	0.313747929383876	0.0
3	4.808397385193502E-4	0.0	0.0	0.44453633826113925	0.0
4	4.808397385193502E-4	0.0	0.0	0.625332079944415	0.0
5	4.808397385193502E-4	0.0	0.0	1.164834266563126	0.0
6	4.808397385193502E-4	0.0	0.0	1.519213153851887	0.0
7	4.808397385193502E-4	0.0	0.0	1.8594072688543273	0.0
8	4.808397385193502E-4	0.0	0.0	2.5051750376858144	0.0
9	4.808397385193502E-4	0.0	0.0	2.781657887334441	0.0
10	4.808397385193502E-4	0.0	0.0	3.4745479505408245	0.0
11	4.808397385193502E-4	0.0	0.0	4.2388427149173316	0.0
12	4.808397385193502E-4	0.0	0.0	4.883167964533261	0.0
13	4.808397385193502E-4	0.0	0.0	5.135849247125179	0.0
14	4.808397385193502E-4	0.0	0.0	5.237787271691282	0.0
15	4.808397385193502E-4	0.0	0.0	5.441663320823486	0.0
16	4.808397385193502E-4	0.0	0.0	5.855666335688647	0.0
17	4.808397385193502E-4	0.0	0.0	6.3586247021798865	0.0
18	4.808397385193502E-4	0.0	0.0	6.912792500823438	0.0
19	4.808397385193502E-4	0.0	0.0	7.231108407723248	0.0
20	4.808397385193502E-4	0.0	0.0	7.534518282728958	0.0
21	4.808397385193502E-4	0.0	0.0	7.915102935767024	0.0
22	4.808397385193502E-4	0.0	0.0	8.3021789252751	0.0
23	4.808397385193502E-4	0.0	0.0	8.710652283147288	0.0
24	4.808397385193502E-4	0.0	0.0	9.03497868677859	0.0
25	4.808397385193502E-4	0.0	0.0	9.32324211002094	0.0
26	4.808397385193502E-4	0.0	0.0	9.593954882807335	0.0
27	4.808397385193502E-4	0.0	0.0	9.859137998600756	0.0
28	4.808397385193502E-4	0.0	0.0	10.140188825765316	0.0
29	4.808397385193502E-4	0.0	0.0	10.443598700771027	0.0
30	4.808397385193502E-4	0.0	0.0	10.779705677996052	0.0
31	4.808397385193502E-4	0.0	0.0	11.120861472475532	0.0
32	4.808397385193502E-4	0.0	0.0	11.427156385912358	0.0
33	4.808397385193502E-4	0.0	0.0	11.724315344317315	0.0
34	4.808397385193502E-4	0.0	0.0	12.044314190301943	0.0
35	4.808397385193502E-4	0.0	0.0	12.366476815109907	0.0
36	4.808397385193502E-4	0.0	0.0	12.689360699525652	0.0
37	4.808397385193502E-4	0.0	0.0	13.01609130184955	0.0
38	4.808397385193502E-4	0.0	0.0	13.367825570576455	0.0
39	4.808397385193502E-4	0.0	0.0	13.720040679041878	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGCG	25	3.8864975E-5	45.000004	1
CGGATGG	50	2.1827873E-11	45.000004	2
CTACGAA	25	3.8864975E-5	45.000004	11
TACGAAT	25	3.8864975E-5	45.000004	12
CGAATAT	25	3.8864975E-5	45.000004	14
CGTCGTC	25	3.8864975E-5	45.000004	37
TAATACG	20	7.028091E-4	45.0	4
CGAAAGT	20	7.028091E-4	45.0	20
CGCATCG	20	7.028091E-4	45.0	21
ACGATTG	20	7.028091E-4	45.0	17
TTGACGA	20	7.028091E-4	45.0	14
GCGATTC	20	7.028091E-4	45.0	9
AGTCGTG	20	7.028091E-4	45.0	1
GTACGAA	20	7.028091E-4	45.0	1
CGACAAT	30	2.162149E-6	44.999996	20
TCATCGA	30	2.162149E-6	44.999996	16
TCGACAA	30	2.162149E-6	44.999996	19
GGTACAT	55	6.002665E-11	40.909092	8
AGACACG	100	0.0	40.500004	24
GCGATCA	45	1.9232175E-8	40.0	9
>>END_MODULE