##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936083.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	175650
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.751710788499857	31.0	31.0	34.0	30.0	34.0
2	31.89029319669798	31.0	31.0	34.0	30.0	34.0
3	31.852211784799316	33.0	31.0	34.0	30.0	34.0
4	35.59638485624822	37.0	35.0	37.0	33.0	37.0
5	31.140563620836893	37.0	35.0	37.0	0.0	37.0
6	33.328323370338744	37.0	35.0	37.0	17.0	37.0
7	29.46719612866496	35.0	32.0	37.0	0.0	37.0
8	32.514489040705946	35.0	33.0	37.0	17.0	37.0
9	36.38061485909479	39.0	35.0	39.0	32.0	39.0
10	36.667856532877884	38.0	35.0	39.0	32.0	39.0
11	36.828454312553376	39.0	37.0	39.0	33.0	39.0
12	36.65672075149445	38.0	35.0	39.0	33.0	39.0
13	36.263672075149444	38.0	35.0	39.0	32.0	39.0
14	37.43598633646456	39.0	36.0	41.0	33.0	41.0
15	37.60263592371193	39.0	36.0	41.0	33.0	41.0
16	37.73835468260746	39.0	36.0	41.0	33.0	41.0
17	37.74515229148876	39.0	36.0	41.0	33.0	41.0
18	37.64319385140905	39.0	36.0	41.0	33.0	41.0
19	37.54615997722744	39.0	36.0	41.0	33.0	41.0
20	37.480626245374324	39.0	35.0	41.0	33.0	41.0
21	37.39346996868773	39.0	35.0	41.0	33.0	41.0
22	37.368021633931114	39.0	35.0	41.0	33.0	41.0
23	37.31615713065756	39.0	35.0	41.0	33.0	41.0
24	37.2801024765158	39.0	35.0	41.0	32.0	41.0
25	37.16699117563336	39.0	35.0	41.0	32.0	41.0
26	36.963649302590376	39.0	35.0	40.0	32.0	41.0
27	36.95173925419869	39.0	35.0	40.0	32.0	41.0
28	36.890964987190436	39.0	35.0	40.0	31.0	41.0
29	36.90596071733561	39.0	35.0	40.0	31.0	41.0
30	36.794659834898944	39.0	35.0	40.0	31.0	41.0
31	36.63353259322516	39.0	35.0	40.0	31.0	41.0
32	36.51806433247936	38.0	35.0	40.0	31.0	41.0
33	36.36750925135212	38.0	35.0	40.0	30.0	41.0
34	36.25759180187874	38.0	35.0	40.0	30.0	41.0
35	36.17519499003701	38.0	35.0	40.0	30.0	41.0
36	35.99797893538286	38.0	35.0	40.0	30.0	41.0
37	35.828858525476804	38.0	35.0	40.0	29.0	41.0
38	35.690253344719615	38.0	35.0	40.0	29.0	41.0
39	35.57417022487902	38.0	35.0	40.0	27.0	41.0
40	35.3575974950185	38.0	34.0	40.0	26.0	41.0
41	35.297107884998574	38.0	34.0	40.0	26.0	41.0
42	35.18649587247367	38.0	34.0	40.0	26.0	41.0
43	34.94328494164532	38.0	34.0	40.0	24.0	41.0
44	34.58438941076003	38.0	34.0	40.0	23.0	41.0
45	34.41310560774267	37.0	33.0	40.0	23.0	41.0
46	34.1577853686308	37.0	33.0	40.0	22.0	41.0
47	33.99320808425847	37.0	33.0	40.0	22.0	41.0
48	33.91914602903501	37.0	33.0	40.0	21.0	41.0
49	33.72788499857671	37.0	33.0	40.0	20.0	41.0
50	33.5402220324509	36.0	33.0	40.0	19.0	41.0
51	31.461855963563906	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	11.0
10	11.0
11	7.0
12	3.0
13	5.0
14	5.0
15	6.0
16	21.0
17	46.0
18	71.0
19	159.0
20	273.0
21	386.0
22	640.0
23	902.0
24	1361.0
25	1708.0
26	2236.0
27	2582.0
28	3115.0
29	3842.0
30	4675.0
31	5656.0
32	7527.0
33	10634.0
34	15142.0
35	17654.0
36	16617.0
37	24523.0
38	29687.0
39	26139.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.786507258753204	22.88812980358668	22.06091659550242	11.264446342157699
2	30.980358667805298	26.840876743524056	24.40193566752064	17.776828921150013
3	30.3535439795047	25.759180187873614	26.305721605465415	17.581554227156275
4	27.472815257614574	27.299743808710502	26.524907486478792	18.702533447196128
5	21.525192143467123	40.78508397381156	21.445488186734984	16.244235695986337
6	23.91460290350128	38.026188442926276	24.275547964702536	13.783660688869912
7	68.67919157415314	19.78992314261315	7.482493595217762	4.048391688015941
8	82.36606888699117	6.157699971534301	6.765157984628523	4.711073156846
9	77.5730145175064	7.256475946484486	8.642755479647025	6.527754056362084
10	42.3643609450612	25.150014232849415	16.917164816396244	15.56846000569314
11	35.053230856817535	24.897808141189866	22.474807856532877	17.57415314545972
12	30.527754056362085	23.792769712496444	27.042983205237686	18.636493025903786
13	22.99459151722175	29.88101337887845	29.612297181895812	17.512097922003985
14	19.41019072018218	32.19869057785368	28.038144036436098	20.35297466552804
15	17.70281810418446	25.88385994876174	38.77711357813835	17.636208368915458
16	19.524053515513806	24.83518360375747	34.45772843723313	21.18303444349559
17	19.8912610304583	23.59635639054939	28.232849416453178	28.27953316253914
18	21.381725021349276	26.2732707087959	30.818673498434386	21.526330771420437
19	23.6356390549388	28.478793054369483	25.969257045260463	21.916310845431255
20	25.576999715343014	27.292912040990608	27.421007685738687	19.709080557927695
21	22.386564190150867	29.414745231995443	27.661258183888414	20.537432393965272
22	21.91858810133789	26.597210361514374	26.162823797324226	25.321377739823514
23	20.251067463706235	30.079134642755477	26.782237403928267	22.88756048961002
24	21.841161400512384	24.84144605750071	30.69911756333618	22.618274978650728
25	18.327924850555082	28.66268146883006	29.176771989752346	23.83262169086251
26	18.41616851693709	31.731283803017362	27.53202391118702	22.320523768858525
27	19.78081411898662	31.759180187873614	27.791631084543127	20.66837460859664
28	16.259607173356105	28.946769143182465	33.301451750640474	21.49217193282095
29	18.491317961855962	27.243381725021347	32.02960432678622	22.235695986336466
30	20.277255906632508	29.788215200683176	28.53800170794193	21.396527184742386
31	23.24964417876459	29.583831483062912	24.686023341873042	22.48050099629946
32	23.10560774267008	30.086535724452034	25.484201537147737	21.323654995730145
33	21.87247366922858	29.30031312268716	24.97637346996869	23.85083973811557
34	20.743524053515515	28.405351551380587	27.42271562766866	23.42840876743524
35	19.521206945630514	27.725021349274126	27.190435525192143	25.563336179903217
36	22.319385140905208	29.760888129803586	25.816680899516083	22.10304582977512
37	20.51693709080558	29.808141189866213	28.37688585254768	21.29803586678053
38	18.891545687446627	29.973811557073727	24.96384856248221	26.170794192997437
39	20.63193851409052	26.802732707087962	28.120694563051522	24.444634215769998
40	22.196413321947052	25.40108169655565	26.916026188442927	25.486478793054367
41	18.798178195274694	26.610873896954168	26.04554511813265	28.545402789638487
42	20.56475946484486	26.749217193282092	27.017364076288075	25.66865926558497
43	21.808710503842867	27.093652149160263	25.91004839168802	25.187588955308854
44	19.573014517506405	28.922857956162822	27.439225732991744	24.06490179333903
45	20.245943637916312	29.010532308568177	25.3378878451466	25.405636208368914
46	21.932251636777682	29.134073441502988	25.36578423000285	23.56789069171648
47	19.567321377739823	27.27981781952747	28.648448619413607	24.5044121833191
48	20.314830629091947	25.323085681753486	28.890976373469968	25.4711073156846
49	20.170794192997437	23.96697978935383	30.252206091659552	25.61001992598918
50	19.0577853686308	26.107031027611725	29.40165101053231	25.43353259322516
51	18.727583262169087	26.89723882721321	25.482493595217765	28.89268431539994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	203.0
1	277.0
2	351.0
3	436.5
4	522.0
5	447.0
6	372.0
7	355.5
8	339.0
9	353.5
10	368.0
11	428.5
12	489.0
13	484.5
14	480.0
15	467.0
16	454.0
17	464.0
18	474.0
19	545.0
20	616.0
21	611.0
22	606.0
23	662.5
24	719.0
25	810.0
26	1065.5
27	1230.0
28	1726.0
29	2222.0
30	2320.0
31	2418.0
32	2950.0
33	3482.0
34	4108.0
35	4734.0
36	5187.5
37	5641.0
38	6339.0
39	7037.0
40	8297.5
41	9558.0
42	11380.0
43	13202.0
44	14713.5
45	16225.0
46	17720.5
47	19216.0
48	18906.5
49	18597.0
50	17316.0
51	16035.0
52	13987.5
53	11940.0
54	10674.5
55	9409.0
56	8288.0
57	7167.0
58	6462.5
59	5758.0
60	5456.5
61	5155.0
62	4325.0
63	3495.0
64	2935.5
65	2376.0
66	1883.5
67	1391.0
68	1146.5
69	902.0
70	765.0
71	628.0
72	485.0
73	342.0
74	299.5
75	205.0
76	153.0
77	116.0
78	79.0
79	69.0
80	59.0
81	40.5
82	22.0
83	14.5
84	7.0
85	9.0
86	11.0
87	7.5
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	175650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.30885283233704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03060258702283	32.927981781952745
2	8.829792827847301	7.6481639624252775
3	3.771427069092439	4.900085397096499
4	2.3412030707750553	4.055792769712497
5	1.5616784099274372	3.3817250213492747
6	1.088442528131244	2.828351836037575
7	0.7860973814281207	2.3831483062909196
8	0.6480702492375644	2.245374323939653
9	0.5087285729309076	1.9829205807002561
>10	4.376117362498685	35.03444349558782
>50	0.04206541171521716	1.2217477939083403
>100	0.015774529393206435	1.390264730999146
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCG	424	0.2413891261030458	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGC	419	0.2385425562197552	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTC	239	0.13606604042129233	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	233	0.13265015656134357	TruSeq Adapter, Index 15 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	218	0.12411044691147168	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15997722744093368	0.0
2	0.0	0.0	0.0	0.802732707087959	0.0
3	0.0	0.0	0.0	1.0600626245374325	0.0
4	0.0	0.0	0.0	1.4198690577853685	0.0
5	0.0	0.0	0.0	2.546541417591802	0.0
6	0.0	0.0	0.0	3.13065755764304	0.0
7	0.0	0.0	0.0	3.7739823512667234	0.0
8	0.0	0.0	0.0	4.920011386279533	0.0
9	0.0	0.0	0.0	5.371477369769428	0.0
10	0.0	0.0	0.0	6.953031596925705	0.0
11	0.0	0.0	0.0	8.339311130088245	0.0
12	0.0	0.0	0.0	9.91175633361799	0.0
13	0.0	0.0	0.0	10.393965271847424	0.0
14	0.0	0.0	0.0	10.577853686307998	0.0
15	0.0	0.0	0.0	10.959863364645601	0.0
16	0.0	0.0	0.0	11.71762026757757	0.0
17	0.0	0.0	0.0	12.678052946199829	0.0
18	0.0	0.0	0.0	13.900370054084828	0.0
19	5.69313976658127E-4	0.0	0.0	14.494164531739255	0.0
20	5.69313976658127E-4	0.0	0.0	14.984343865641902	0.0
21	5.69313976658127E-4	0.0	0.0	15.643040136635355	5.69313976658127E-4
22	5.69313976658127E-4	0.0	0.0	16.300028465698833	5.69313976658127E-4
23	5.69313976658127E-4	0.0	0.0	17.007685738684884	5.69313976658127E-4
24	5.69313976658127E-4	0.0	0.0	17.539424992883575	5.69313976658127E-4
25	5.69313976658127E-4	0.0	0.0	17.990321662396813	5.69313976658127E-4
26	5.69313976658127E-4	0.0	0.0	18.414460575007116	5.69313976658127E-4
27	5.69313976658127E-4	0.0	0.0	18.846000569313976	5.69313976658127E-4
28	5.69313976658127E-4	0.0	0.0	19.289496157130657	5.69313976658127E-4
29	5.69313976658127E-4	0.0	0.0	19.705664674067748	5.69313976658127E-4
30	5.69313976658127E-4	0.0	0.0	20.229433532593227	5.69313976658127E-4
31	5.69313976658127E-4	0.0	0.0	20.742954739538856	5.69313976658127E-4
32	5.69313976658127E-4	0.0	0.0	21.21263877028181	5.69313976658127E-4
33	5.69313976658127E-4	0.0	0.0	21.67150583546826	5.69313976658127E-4
34	5.69313976658127E-4	0.0	0.0	22.13663535439795	5.69313976658127E-4
35	5.69313976658127E-4	0.0	0.0	22.62055223455736	5.69313976658127E-4
36	5.69313976658127E-4	0.0	0.0	23.049814972957588	5.69313976658127E-4
37	5.69313976658127E-4	0.0	0.0	23.530315969257046	5.69313976658127E-4
38	5.69313976658127E-4	0.0	0.0	23.976088812980358	5.69313976658127E-4
39	5.69313976658127E-4	0.0	0.0	24.41104469114717	5.69313976658127E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	20	7.0169574E-4	45.000004	5
CTATGCG	20	7.0169574E-4	45.000004	1
CCCTAGA	20	7.0169574E-4	45.000004	23
CGCGCAG	20	7.0169574E-4	45.000004	35
AATGGTC	45	3.8016879E-10	45.000004	10
TCGATCA	20	7.0169574E-4	45.000004	17
ATACTCG	20	7.0169574E-4	45.000004	10
GTTACGG	20	7.0169574E-4	45.000004	2
CCGTACG	20	7.0169574E-4	45.000004	1
CGGGCTA	20	7.0169574E-4	45.000004	6
AGCTGTA	20	7.0169574E-4	45.000004	10
ACTCGCA	20	7.0169574E-4	45.000004	12
TCGTAGG	20	7.0169574E-4	45.000004	2
TACTCGC	20	7.0169574E-4	45.000004	11
GTTCTTC	50	2.1827873E-11	45.0	35
TAGAATC	50	2.1827873E-11	45.0	26
AAGACGC	25	3.8772778E-5	45.0	38
TACGGGA	35	1.20408E-7	45.0	4
CAAGACG	25	3.8772778E-5	45.0	37
CCACACG	25	3.8772778E-5	45.0	25
>>END_MODULE