FastQCFastQC Report
Sat 14 Jan 2017
SRR2936082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737930
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56870.7706693046765953No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.25761251067174396No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.1550282547125066No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.10163565649858389No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAC207.031643E-445.028
TCTAGCG207.031643E-445.01
CTACGAC207.031643E-445.012
TACGACT207.031643E-445.013
AGTTACG253.889443E-545.01
GTATACG351.2111377E-745.01
TATACGG502.1827873E-1145.02
CGTTATT5450.042.5229381
CGTTTTT23800.042.352941
CGGTTTT7050.041.8085141
CGTTTTA3250.041.5384641
CGAATAT900.040.014
ACGAACG403.4559707E-739.3751
TTTCGAC356.245249E-638.57142624
GCGCCAA356.245249E-638.5714269
TCGACGT356.245249E-638.57142626
GATTACG356.245249E-638.5714261
TTCGACG356.245249E-638.57142625
CGGTAGT1300.038.0769212
CGTATGG950.037.8947372
Produced by FastQC (version 0.11.5)