##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936082.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	737930
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.822958817231985	31.0	31.0	34.0	30.0	34.0
2	31.934893553589095	33.0	31.0	34.0	30.0	34.0
3	31.59451845025951	33.0	31.0	34.0	30.0	34.0
4	35.48316100443132	37.0	35.0	37.0	33.0	37.0
5	30.97878931605979	37.0	35.0	37.0	0.0	37.0
6	33.297272098979576	37.0	35.0	37.0	17.0	37.0
7	29.740226037700054	35.0	32.0	37.0	0.0	37.0
8	32.75185451194558	35.0	35.0	37.0	17.0	37.0
9	36.5674711693521	39.0	35.0	39.0	32.0	39.0
10	36.850800211402166	38.0	37.0	39.0	33.0	39.0
11	36.97478893662	39.0	37.0	39.0	33.0	39.0
12	37.082927919992414	39.0	37.0	39.0	34.0	39.0
13	36.98547965254157	39.0	37.0	39.0	33.0	39.0
14	38.290809426368355	40.0	38.0	41.0	34.0	41.0
15	38.37385117829604	40.0	38.0	41.0	34.0	41.0
16	38.39080942636836	40.0	38.0	41.0	34.0	41.0
17	38.35418535633461	40.0	38.0	41.0	34.0	41.0
18	38.229117938015804	40.0	37.0	41.0	34.0	41.0
19	38.082818153483394	40.0	37.0	41.0	34.0	41.0
20	37.95220820403019	40.0	36.0	41.0	34.0	41.0
21	37.83345981326142	40.0	36.0	41.0	33.0	41.0
22	37.81523044191184	40.0	36.0	41.0	33.0	41.0
23	37.73858631577521	39.0	36.0	41.0	33.0	41.0
24	37.68737820660496	40.0	36.0	41.0	33.0	41.0
25	37.55361890694239	39.0	35.0	41.0	33.0	41.0
26	37.40756304798558	39.0	35.0	41.0	33.0	41.0
27	37.3631279389644	39.0	35.0	41.0	33.0	41.0
28	37.26050709416882	39.0	35.0	41.0	32.0	41.0
29	37.24419389372976	39.0	35.0	41.0	32.0	41.0
30	37.102104535660565	39.0	35.0	41.0	32.0	41.0
31	36.97453010448145	39.0	35.0	41.0	31.0	41.0
32	36.84049164554904	39.0	35.0	41.0	31.0	41.0
33	36.596224574146596	39.0	35.0	41.0	30.0	41.0
34	36.36701177618473	39.0	35.0	41.0	30.0	41.0
35	36.13851178296044	39.0	35.0	41.0	29.0	41.0
36	35.96660387841665	39.0	35.0	41.0	28.0	41.0
37	35.84060954291058	39.0	35.0	40.0	27.0	41.0
38	35.721531852614746	39.0	35.0	40.0	27.0	41.0
39	35.6984673343	39.0	35.0	40.0	26.0	41.0
40	35.54608296179855	39.0	35.0	40.0	25.0	41.0
41	35.45732522054937	39.0	35.0	40.0	25.0	41.0
42	35.42276367677151	39.0	35.0	40.0	25.0	41.0
43	35.27523477836651	38.0	35.0	40.0	24.0	41.0
44	35.125013212635345	38.0	34.0	40.0	24.0	41.0
45	34.990145406745896	38.0	34.0	40.0	23.0	41.0
46	34.888657460734755	38.0	34.0	40.0	23.0	41.0
47	34.754478067025325	38.0	34.0	40.0	23.0	41.0
48	34.68881872264307	38.0	34.0	40.0	23.0	41.0
49	34.54167332944859	38.0	34.0	40.0	23.0	41.0
50	34.36732481400675	38.0	33.0	40.0	22.0	41.0
51	32.53776103424444	36.0	31.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	16.0
9	38.0
10	35.0
11	31.0
12	24.0
13	21.0
14	27.0
15	34.0
16	78.0
17	167.0
18	296.0
19	581.0
20	1050.0
21	1593.0
22	2570.0
23	4087.0
24	6064.0
25	8755.0
26	11346.0
27	12275.0
28	12510.0
29	13512.0
30	15664.0
31	18944.0
32	24255.0
33	33181.0
34	47231.0
35	58611.0
36	70946.0
37	112794.0
38	142606.0
39	138577.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.81461656254658	20.9880340953749	24.58024473866085	14.617104603417669
2	35.01849768948274	23.79222961527516	24.76915154553955	16.420121149702545
3	31.984199043269683	23.114252029325275	28.913853617551798	15.987695309853239
4	26.57785968858835	25.233965281259742	29.130676351415445	19.057498678736465
5	20.68719255213909	39.65904625099942	24.00065046820159	15.6531107286599
6	24.548940956459283	34.606263466724485	27.303673790196903	13.541121786619328
7	68.7464935698508	18.53780168850704	9.271340100009485	3.444364641632675
8	83.07644356510781	4.491076389359424	9.579770438930522	2.8527096066022524
9	77.06340709823425	7.413440299215372	11.351754231431165	4.171398371119212
10	36.447088477226835	33.143116555770874	18.503110051088857	11.906684915913432
11	26.06751317875679	26.453999701868742	30.62837938557858	16.850107733795888
12	25.12514737170192	23.68219207783936	32.72085428157142	18.471806268887292
13	23.010854688114048	24.20256663911211	33.43393004756548	19.352648625208353
14	19.228111067445422	28.384535118507177	32.05859634382665	20.32875747022075
15	19.041372487905356	27.29987939235429	34.92174054449609	18.737007575244263
16	21.70978277072351	26.794275879826003	33.54572926971393	17.95021207973656
17	21.681189272695242	25.689021993956068	31.535375984171942	21.09441274917675
18	22.440068841218004	26.15586844280623	31.779437073977206	19.624625641998563
19	23.266298971447156	28.269890097976774	29.728971582670443	18.734839347905627
20	24.79693195831583	26.594392422045456	30.634884067594488	17.973791552044233
21	23.360345832260514	27.112598755979565	31.05009960294337	18.47695580881655
22	22.467171682950955	25.476671228978358	30.79939831691353	21.256758771157156
23	21.46070765519765	27.053379046793058	31.74054449609042	19.74536880191888
24	20.910790996435978	25.873456831948833	32.647134552057786	20.56861761955741
25	21.148076375808003	27.874595151301612	30.613066279999458	20.364262192890926
26	20.75034217337688	28.675484124510454	30.45126231485371	20.122911387258956
27	21.2081091702465	28.335072432344532	30.31249576518098	20.14432263222799
28	19.393844944642446	28.286964888268535	31.67834347431328	20.64084669277574
29	20.674589730733267	27.171547436748746	31.133034298646212	21.02082853387178
30	21.07788001571965	26.757687043486513	31.367338365427617	20.797094575366227
31	21.479950672828046	27.539604027482284	29.606060195411487	21.374385104278186
32	21.860474570758743	27.462767471169354	30.24446763243126	20.432290325640643
33	21.020150962828453	28.63930183079696	29.459027279010204	20.881519927364383
34	20.842491835268927	27.583781659507	30.295963031723876	21.277763473500197
35	20.746141232908272	27.79166045559877	29.920182131096446	21.542016180396516
36	20.603038228558265	29.26632607428889	28.857886249373248	21.2727494477796
37	20.830566584906425	29.730191210548423	29.05912484924044	20.3801173553047
38	20.79668803274023	28.682801891778354	29.076606182158198	21.443903893323217
39	21.648665862615697	27.557491903026033	28.642825200222244	22.15101703413603
40	22.313092027699106	27.3517813342729	30.299757429566487	20.035369208461507
41	19.750653856056807	28.487796945509736	29.45347119645495	22.308078001978508
42	20.84235632106026	28.575339124307185	28.27571720894936	22.306587345683194
43	21.189679237868088	27.87798300651823	28.823059097746402	22.109278657867275
44	21.50298808830106	27.89261854105403	28.281002263087284	22.323391107557626
45	20.894935834022196	27.726071578605016	28.722507554917133	22.656485032455652
46	21.220034420609004	28.615722358489286	27.905085848251186	22.259157372650524
47	20.625398072987952	28.303226593308306	29.23000826636673	21.84136706733701
48	20.835309582209693	27.47550580678384	29.68398086539374	22.00520374561273
49	20.685837410052443	27.30109902023227	29.2535877386744	22.759475831040884
50	19.549550770398277	27.574024636483134	30.08645806512813	22.78996652799046
51	19.841448375862207	26.978033146775438	28.75218516661472	24.428333310747632
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	333.0
1	702.5
2	1072.0
3	6863.0
4	12654.0
5	9971.0
6	7288.0
7	5758.0
8	4228.0
9	4018.0
10	3808.0
11	3721.0
12	3634.0
13	3533.0
14	3432.0
15	3352.0
16	3272.0
17	3063.5
18	2855.0
19	3041.0
20	3227.0
21	3550.5
22	3874.0
23	4303.5
24	4733.0
25	5562.5
26	7654.0
27	8916.0
28	10637.0
29	12358.0
30	14551.5
31	16745.0
32	18825.5
33	20906.0
34	23612.0
35	26318.0
36	28714.0
37	31110.0
38	32758.5
39	34407.0
40	37390.0
41	40373.0
42	43846.0
43	47319.0
44	49258.0
45	51197.0
46	54416.5
47	57636.0
48	58696.0
49	59756.0
50	57467.5
51	55179.0
52	49684.5
53	44190.0
54	40221.0
55	36252.0
56	32960.0
57	29668.0
58	28140.0
59	26612.0
60	25550.5
61	24489.0
62	21651.5
63	18814.0
64	15500.5
65	12187.0
66	10271.0
67	8355.0
68	6684.0
69	5013.0
70	4204.5
71	3396.0
72	2856.0
73	2316.0
74	1899.0
75	1236.0
76	990.0
77	770.5
78	551.0
79	409.5
80	268.0
81	203.0
82	138.0
83	103.0
84	68.0
85	56.0
86	44.0
87	37.5
88	31.0
89	24.5
90	18.0
91	18.5
92	19.0
93	11.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	737930.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.979708856237934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5048972389783	29.041792027392997
2	8.476990098338732	6.608612120209112
3	3.9533725973756275	4.6230393853779335
4	2.4369728542630207	3.799699693989108
5	1.6342315033197699	3.185093410154833
6	1.2708751303023857	2.972300554309227
7	0.9931195408803235	2.7098057394068986
8	0.7854444969123952	2.449311824990355
9	0.6307121636936361	2.212647886157924
>10	5.229826257966477	38.21848564262794
>50	0.06213296174165363	1.5189922022932496
>100	0.01963972654202822	1.2411666700621105
>500	7.141718742555518E-4	0.20363651881808173
>1k	7.141718742555518E-4	0.4238367736165817
>5k	3.570859371277759E-4	0.791579550593596
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5687	0.7706693046765953	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1901	0.25761251067174396	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1144	0.1550282547125066	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	750	0.10163565649858389	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.71028417329557E-4	0.0	0.0	0.05596736817855352	0.0
2	2.71028417329557E-4	0.0	0.0	0.2086918813437589	0.0
3	2.71028417329557E-4	0.0	0.0	0.33187429702004256	0.0
4	2.71028417329557E-4	0.0	0.0	0.4679305625194802	0.0
5	2.71028417329557E-4	0.0	0.0	0.907945198054016	0.0
6	2.71028417329557E-4	0.0	0.0	1.338609353190682	0.0
7	2.71028417329557E-4	0.0	0.0	1.6679088802460937	0.0
8	2.71028417329557E-4	0.0	0.0	2.2844985296708358	0.0
9	2.71028417329557E-4	0.0	0.0	2.5926578401745424	0.0
10	2.71028417329557E-4	0.0	0.0	3.1916306424728633	0.0
11	2.71028417329557E-4	0.0	0.0	3.822449283807407	0.0
12	2.71028417329557E-4	0.0	0.0	4.329949995257003	0.0
13	2.71028417329557E-4	0.0	0.0	4.615342918705026	0.0
14	2.71028417329557E-4	0.0	0.0	4.752754326291112	0.0
15	2.71028417329557E-4	0.0	0.0	4.9253994281300395	0.0
16	4.065426259943355E-4	0.0	0.0	5.317713062214573	0.0
17	4.065426259943355E-4	0.0	0.0	5.734148225441437	0.0
18	4.065426259943355E-4	0.0	0.0	6.3375929966256965	0.0
19	4.065426259943355E-4	0.0	0.0	6.6080793571205945	0.0
20	4.065426259943355E-4	0.0	0.0	6.933584486333392	0.0
21	4.065426259943355E-4	0.0	0.0	7.28036534630656	0.0
22	4.065426259943355E-4	0.0	0.0	7.671459352513111	0.0
23	4.065426259943355E-4	0.0	0.0	8.075969265377475	0.0
24	4.065426259943355E-4	0.0	0.0	8.389955686853767	0.0
25	4.065426259943355E-4	0.0	0.0	8.67304486875449	0.0
26	5.42056834659114E-4	0.0	0.0	8.943260200832057	0.0
27	5.42056834659114E-4	0.0	0.0	9.226755925358773	0.0
28	5.42056834659114E-4	0.0	0.0	9.518382502405377	0.0
29	5.42056834659114E-4	0.0	0.0	9.821527787188487	0.0
30	5.42056834659114E-4	0.0	0.0	10.177930155976854	0.0
31	5.42056834659114E-4	0.0	0.0	10.507365197240931	0.0
32	5.42056834659114E-4	0.0	0.0	10.833818925914382	0.0
33	5.42056834659114E-4	0.0	0.0	11.167319393438403	0.0
34	5.42056834659114E-4	0.0	0.0	11.479679644410716	0.0
35	5.42056834659114E-4	0.0	0.0	11.813180111934736	0.0
36	5.42056834659114E-4	0.0	0.0	12.148984321006058	0.0
37	5.42056834659114E-4	0.0	0.0	12.489531527380645	0.0
38	5.42056834659114E-4	0.0	0.0	12.837667529440461	0.0
39	5.42056834659114E-4	0.0	0.0	13.191901670890193	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGAC	20	7.031643E-4	45.0	28
TCTAGCG	20	7.031643E-4	45.0	1
CTACGAC	20	7.031643E-4	45.0	12
TACGACT	20	7.031643E-4	45.0	13
AGTTACG	25	3.889443E-5	45.0	1
GTATACG	35	1.2111377E-7	45.0	1
TATACGG	50	2.1827873E-11	45.0	2
CGTTATT	545	0.0	42.522938	1
CGTTTTT	2380	0.0	42.35294	1
CGGTTTT	705	0.0	41.808514	1
CGTTTTA	325	0.0	41.538464	1
CGAATAT	90	0.0	40.0	14
ACGAACG	40	3.4559707E-7	39.375	1
TTTCGAC	35	6.245249E-6	38.571426	24
GCGCCAA	35	6.245249E-6	38.571426	9
TCGACGT	35	6.245249E-6	38.571426	26
GATTACG	35	6.245249E-6	38.571426	1
TTCGACG	35	6.245249E-6	38.571426	25
CGGTAGT	130	0.0	38.07692	12
CGTATGG	95	0.0	37.894737	2
>>END_MODULE