FastQCFastQC Report
Sat 14 Jan 2017
SRR2936081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826019
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37170.44998964914850625No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12050.14588042163679044No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG11700.14164323096684217No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC10980.13292672444580572No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC9710.117551775443422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG1300.045.0000042
CTAAGCG207.032133E-445.01
CGTTCGG253.88985E-545.02
CGAATGA207.032133E-445.028
CGCCGAA207.032133E-445.030
GCATCCG207.032133E-445.031
TAATGCG302.1647575E-644.9999961
CGTTATT3700.042.5675661
GTAACGG900.042.52
CGGTTTT4700.042.127661
CTACGAA1450.041.8965511
TACGGGA4500.041.54
TTAACGG603.6379788E-1241.2499962
TAGTACG501.0804797E-940.51
CGAATAT1450.040.34482614
CGATTGA451.9272193E-840.010
TACGGGT1350.040.04
CGTTTTA1300.039.8076931
CGTTTTT16200.039.5833321
ACGGGAT6200.039.556455
Produced by FastQC (version 0.11.5)