##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936081.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	826019
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81109030179693	31.0	31.0	34.0	30.0	34.0
2	31.931690433270905	33.0	31.0	34.0	30.0	34.0
3	31.731044927537987	33.0	31.0	34.0	30.0	34.0
4	35.54699104984268	37.0	35.0	37.0	33.0	37.0
5	30.97276697025129	37.0	35.0	37.0	0.0	37.0
6	33.278295051324484	37.0	35.0	37.0	17.0	37.0
7	29.728002624636964	35.0	32.0	37.0	0.0	37.0
8	32.71528378887169	35.0	35.0	37.0	17.0	37.0
9	36.518532866677404	39.0	35.0	39.0	32.0	39.0
10	36.77521824558515	38.0	35.0	39.0	33.0	39.0
11	36.90254340396528	39.0	37.0	39.0	33.0	39.0
12	37.02522096949344	39.0	37.0	39.0	33.0	39.0
13	36.91579249387726	39.0	37.0	39.0	33.0	39.0
14	38.186343171282985	40.0	37.0	41.0	33.0	41.0
15	38.26161383696985	40.0	37.0	41.0	33.0	41.0
16	38.27664375758911	40.0	37.0	41.0	34.0	41.0
17	38.228234459497905	40.0	37.0	41.0	33.0	41.0
18	38.131378333912416	40.0	37.0	41.0	33.0	41.0
19	38.00701557712353	40.0	37.0	41.0	33.0	41.0
20	37.92106719094839	40.0	37.0	41.0	33.0	41.0
21	37.83625800375052	40.0	36.0	41.0	33.0	41.0
22	37.83344814102339	40.0	36.0	41.0	33.0	41.0
23	37.74901909035991	39.0	36.0	41.0	33.0	41.0
24	37.693593004519265	39.0	36.0	41.0	33.0	41.0
25	37.570170904059104	39.0	36.0	41.0	33.0	41.0
26	37.41286096324661	39.0	36.0	41.0	32.0	41.0
27	37.35882830782343	39.0	36.0	41.0	32.0	41.0
28	37.242391518839156	39.0	36.0	41.0	32.0	41.0
29	37.227729628495226	39.0	36.0	41.0	32.0	41.0
30	37.093606805654595	39.0	35.0	41.0	31.0	41.0
31	36.98478243236536	39.0	35.0	41.0	31.0	41.0
32	36.86772459229146	39.0	35.0	41.0	31.0	41.0
33	36.71006478059221	39.0	35.0	41.0	30.0	41.0
34	36.54941230165408	39.0	35.0	41.0	30.0	41.0
35	36.42761001865575	39.0	35.0	41.0	30.0	41.0
36	36.29877884164892	39.0	35.0	40.0	30.0	41.0
37	36.14712736631966	39.0	35.0	40.0	29.0	41.0
38	36.04648561352705	39.0	35.0	40.0	29.0	41.0
39	35.9986828390146	39.0	35.0	40.0	28.0	41.0
40	35.8626992841569	39.0	35.0	40.0	27.0	41.0
41	35.77820001719089	39.0	35.0	40.0	27.0	41.0
42	35.707717376961064	39.0	35.0	40.0	27.0	41.0
43	35.56621699985109	38.0	35.0	40.0	26.0	41.0
44	35.3838652621792	38.0	35.0	40.0	25.0	41.0
45	35.28683843834101	38.0	34.0	40.0	25.0	41.0
46	35.13693631744548	38.0	34.0	40.0	24.0	41.0
47	34.975565937345266	38.0	34.0	40.0	24.0	41.0
48	34.891383854366545	38.0	34.0	40.0	23.0	41.0
49	34.737443085449605	38.0	34.0	40.0	23.0	41.0
50	34.578906780594636	38.0	34.0	40.0	23.0	41.0
51	32.59676593395552	36.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	27.0
10	39.0
11	32.0
12	16.0
13	20.0
14	24.0
15	37.0
16	81.0
17	165.0
18	308.0
19	574.0
20	1074.0
21	1705.0
22	2581.0
23	4055.0
24	5839.0
25	8346.0
26	10470.0
27	12062.0
28	13343.0
29	15195.0
30	18248.0
31	22279.0
32	28387.0
33	38148.0
34	53260.0
35	66830.0
36	81902.0
37	127715.0
38	159996.0
39	153227.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.89482203193873	20.849157222775748	25.493844572582468	12.762176172703048
2	32.51123763496965	25.54384342248786	25.39336262240941	16.55155632013307
3	30.939360959009417	25.22617518483231	27.875387854274535	15.959076001883735
4	27.48290293564676	25.820229316759058	28.758055202120048	17.938812545474136
5	21.55337831212115	39.42003755361559	22.87283948674304	16.153744647520217
6	24.576069073471675	35.807408788417696	25.39324155981884	14.223280578291783
7	69.85275157108977	18.506596095247204	7.924999303890104	3.715653029772923
8	83.96864963154601	4.82519167234652	8.073664165110003	3.132494530997471
9	77.77714556202703	7.678031619127405	9.562612966529825	4.982209852315746
10	36.12713509011294	34.803436724821104	16.68678323379971	12.382644951266254
11	25.787784542486307	27.370556851597843	29.85052401942328	16.991134586492564
12	24.995550949796556	24.216634242069492	31.608110709317828	19.179704098816128
13	22.60686497526086	25.103538780584948	33.51884157628335	18.770754667870836
14	19.42146609218432	28.80333261099321	30.93815033310372	20.837050963718752
15	18.985156515770218	28.36375434463372	34.35913701742938	18.291952122166684
16	22.38144643161961	27.180367521812453	31.897329238189435	18.5408568083785
17	21.791145240000535	26.81645337455918	30.597359140649306	20.795042244790977
18	22.490402763132565	27.157244567013596	30.582105254237497	19.77024741561635
19	22.938092223060245	28.673553513902224	29.1752368892241	19.213117373813436
20	24.210217924769285	27.988702439048012	29.340366262761506	18.460713373421193
21	23.171258772497968	28.038338101181694	29.822074310639344	18.968328815680994
22	22.68386078286335	25.25135620367086	29.5660269315839	22.498756081881893
23	20.96513518454176	27.656627753114638	30.630530290465472	20.74770677187813
24	20.49759145976061	26.493458382918554	31.884617666179594	21.124332491141246
25	20.854605039351394	28.52973115630512	29.449806844636743	21.165856959706737
26	20.501223337477708	29.23274161974482	29.50646413702348	20.759570905753986
27	21.072033452015027	28.572466250776312	29.794593102579963	20.560907194628694
28	19.357666106953957	28.316903122083147	31.64418736130767	20.681243409655224
29	20.79262099297958	26.806889429904157	30.720842983030654	21.679646594085607
30	20.827729144244866	27.932166209251847	30.226060175371266	21.01404447113202
31	21.21210286930446	28.1700541997218	28.849941708362643	21.7679012226111
32	21.06525394694311	29.197633468479538	28.788078724581396	20.949033859995954
33	20.347473847453873	29.493631502423067	28.36145415541289	21.79744049471017
34	19.335753778060795	28.490264751779314	29.765780206024317	22.40820126413557
35	19.56904139008909	28.7082984773958	28.06993543732045	23.652724695194664
36	20.062129321480498	29.892169550579347	28.21823711076864	21.82746401717152
37	20.20704124239273	29.700164281935404	28.712777793246886	21.38001668242498
38	19.856080792330445	29.19569646703042	28.122839789399517	22.82538295123962
39	20.807632754210253	27.25554739055639	28.917736759081812	23.01908309615154
40	20.8682851120858	26.89768637283162	29.889869361358517	22.344159153724068
41	18.95368024222203	27.52951203301619	29.17511582663353	24.34169189812825
42	20.116244299465265	28.07259881431299	28.809506803112278	23.001650083109467
43	20.8142911966916	27.218623300432554	28.835414197494245	23.131671305381595
44	20.316118636496256	28.18446064799962	28.718951985365955	22.780468730138168
45	20.807995941981964	27.416802761195562	27.94221440426915	23.83298689255332
46	20.308975943652626	28.71459373210544	27.497067258743442	23.479363065498493
47	19.649426950227536	27.630236108370386	29.888779798043387	22.831557143358687
48	20.25546627862071	26.933278774459186	29.34690364265229	23.46435130426782
49	20.000387400289824	27.0629368089596	29.45549678639353	23.481179004357042
50	19.216022875987115	27.00833758061255	29.418330571088557	24.357308972311774
51	18.828743648753843	26.555563491880935	29.005749262426168	25.609943596939054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	282.0
1	612.5
2	943.0
3	4703.0
4	8463.0
5	6601.0
6	4739.0
7	3852.0
8	2965.0
9	2799.5
10	2634.0
11	2655.0
12	2676.0
13	2602.5
14	2529.0
15	2482.5
16	2436.0
17	2522.5
18	2609.0
19	2872.5
20	3136.0
21	3604.5
22	4073.0
23	4263.5
24	4454.0
25	5742.5
26	8961.5
27	10892.0
28	12958.0
29	15024.0
30	17573.5
31	20123.0
32	23087.5
33	26052.0
34	28490.5
35	30929.0
36	32936.0
37	34943.0
38	38517.5
39	42092.0
40	45338.5
41	48585.0
42	52245.0
43	55905.0
44	60121.0
45	64337.0
46	68869.5
47	73402.0
48	72051.5
49	70701.0
50	67074.5
51	63448.0
52	56426.0
53	49404.0
54	43718.0
55	38032.0
56	34925.5
57	31819.0
58	29509.0
59	27199.0
60	25835.5
61	24472.0
62	21643.0
63	18814.0
64	15288.5
65	11763.0
66	9164.5
67	6566.0
68	5495.5
69	4425.0
70	3776.0
71	3127.0
72	2634.5
73	2142.0
74	1634.5
75	927.0
76	727.0
77	594.0
78	461.0
79	335.5
80	210.0
81	159.5
82	109.0
83	95.5
84	82.0
85	70.5
86	59.0
87	45.5
88	32.0
89	20.0
90	8.0
91	17.0
92	26.0
93	18.0
94	10.0
95	6.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	826019.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.13346223827956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95301725915665	29.33171070556323
2	8.649496910727025	6.769695214721036
3	3.7572910161817115	4.411074182999223
4	2.323109150689508	3.6364521689563833
5	1.651617526822078	3.2316756058986242
6	1.2500777371504312	2.935192195301423
7	0.9204103111355055	2.5213189508181113
8	0.7408596701652715	2.319392314102153
9	0.5679967990712541	2.0004913159126727
>10	5.101683689745556	38.8054345349381
>50	0.06301489913378316	1.5736718313001223
>100	0.018589364283133063	1.1661652846930826
>500	0.0015753698544998115	0.41120334367165695
>1k	0.0012602958835998491	0.886522351124202
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3717	0.44998964914850625	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1205	0.14588042163679044	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG	1170	0.14164323096684217	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC	1098	0.13292672444580572	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC	971	0.117551775443422	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2106259056995057E-4	0.0	0.0	0.08220149899699644	0.0
2	1.2106259056995057E-4	0.0	0.0	0.3313483103899547	0.0
3	1.2106259056995057E-4	0.0	0.0	0.48037635938156387	0.0
4	1.2106259056995057E-4	0.0	0.0	0.688240827390169	0.0
5	1.2106259056995057E-4	0.0	0.0	1.3705495878424103	0.0
6	1.2106259056995057E-4	0.0	0.0	1.9264690037396235	0.0
7	1.2106259056995057E-4	0.0	0.0	2.4138669933742443	0.0
8	1.2106259056995057E-4	0.0	0.0	3.2529518086145717	0.0
9	1.2106259056995057E-4	0.0	0.0	3.6116602644733353	0.0
10	1.2106259056995057E-4	0.0	0.0	4.5242300721896225	0.0
11	1.2106259056995057E-4	0.0	0.0	5.345518686616168	0.0
12	1.2106259056995057E-4	0.0	0.0	6.143684346243852	0.0
13	1.2106259056995057E-4	0.0	0.0	6.468253151561889	0.0
14	1.2106259056995057E-4	0.0	0.0	6.631445523650182	0.0
15	1.2106259056995057E-4	0.0	0.0	6.867638637852156	0.0
16	1.2106259056995057E-4	0.0	0.0	7.308306467526776	0.0
17	1.2106259056995057E-4	0.0	0.0	7.865073321557979	0.0
18	1.2106259056995057E-4	0.0	0.0	8.468570335549183	0.0
19	1.2106259056995057E-4	0.0	0.0	8.846769868489709	0.0
20	1.2106259056995057E-4	0.0	0.0	9.201604321450233	0.0
21	1.2106259056995057E-4	0.0	0.0	9.647235717338221	0.0
22	1.2106259056995057E-4	0.0	0.0	10.148313779707246	0.0
23	1.2106259056995057E-4	0.0	0.0	10.698301128666532	0.0
24	1.2106259056995057E-4	0.0	0.0	11.107613747383535	0.0
25	1.2106259056995057E-4	0.0	0.0	11.491745347261988	0.0
26	1.2106259056995057E-4	0.0	0.0	11.837621168520336	0.0
27	1.2106259056995057E-4	0.0	0.0	12.183133802006976	0.0
28	1.2106259056995057E-4	0.0	0.0	12.573318531413927	0.0
29	1.2106259056995057E-4	0.0	0.0	12.988563217068856	0.0
30	1.2106259056995057E-4	0.0	0.0	13.438552866217362	0.0
31	1.2106259056995057E-4	0.0	0.0	13.856824116636542	0.0
32	2.4212518113990114E-4	0.0	0.0	14.273642615968882	0.0
33	2.4212518113990114E-4	0.0	0.0	14.66818559863635	0.0
34	3.6318777170985173E-4	0.0	0.0	15.058007140271592	0.0
35	3.6318777170985173E-4	0.0	0.0	15.485115959802377	0.0
36	3.6318777170985173E-4	0.0	0.0	15.896123454787359	0.0
37	3.6318777170985173E-4	0.0	0.0	16.31439470520654	0.0
38	3.6318777170985173E-4	0.0	0.0	16.745014339863854	0.0
39	3.6318777170985173E-4	0.0	0.0	17.160016900337645	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACGG	130	0.0	45.000004	2
CTAAGCG	20	7.032133E-4	45.0	1
CGTTCGG	25	3.88985E-5	45.0	2
CGAATGA	20	7.032133E-4	45.0	28
CGCCGAA	20	7.032133E-4	45.0	30
GCATCCG	20	7.032133E-4	45.0	31
TAATGCG	30	2.1647575E-6	44.999996	1
CGTTATT	370	0.0	42.567566	1
GTAACGG	90	0.0	42.5	2
CGGTTTT	470	0.0	42.12766	1
CTACGAA	145	0.0	41.89655	11
TACGGGA	450	0.0	41.5	4
TTAACGG	60	3.6379788E-12	41.249996	2
TAGTACG	50	1.0804797E-9	40.5	1
CGAATAT	145	0.0	40.344826	14
CGATTGA	45	1.9272193E-8	40.0	10
TACGGGT	135	0.0	40.0	4
CGTTTTA	130	0.0	39.807693	1
CGTTTTT	1620	0.0	39.583332	1
ACGGGAT	620	0.0	39.55645	5
>>END_MODULE