##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936080.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	788592
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848164830482684	31.0	31.0	34.0	30.0	34.0
2	31.958592022237102	33.0	31.0	34.0	30.0	34.0
3	31.57966730578043	33.0	31.0	34.0	30.0	34.0
4	35.48439370422221	37.0	35.0	37.0	33.0	37.0
5	31.01944224643415	37.0	35.0	37.0	0.0	37.0
6	33.328278755046966	37.0	35.0	37.0	17.0	37.0
7	29.67614051372573	35.0	32.0	37.0	0.0	37.0
8	32.720561456367804	35.0	35.0	37.0	17.0	37.0
9	36.59197912228377	39.0	35.0	39.0	32.0	39.0
10	36.902868657049524	38.0	37.0	39.0	34.0	39.0
11	37.033288443200036	39.0	37.0	39.0	34.0	39.0
12	37.11325121228722	39.0	37.0	39.0	34.0	39.0
13	36.99581405847384	39.0	37.0	39.0	33.0	39.0
14	38.26070895976627	40.0	38.0	41.0	33.0	41.0
15	38.354022105220444	40.0	38.0	41.0	34.0	41.0
16	38.38525752226754	40.0	38.0	41.0	34.0	41.0
17	38.349127305374644	40.0	38.0	41.0	34.0	41.0
18	38.21277289143182	40.0	37.0	41.0	34.0	41.0
19	38.06891649927973	40.0	37.0	41.0	34.0	41.0
20	37.92580193560168	40.0	36.0	41.0	34.0	41.0
21	37.80548876985818	40.0	36.0	41.0	33.0	41.0
22	37.78430417757218	40.0	36.0	41.0	33.0	41.0
23	37.70702086757157	39.0	36.0	41.0	33.0	41.0
24	37.657183943027576	39.0	35.0	41.0	33.0	41.0
25	37.53107437052367	39.0	35.0	41.0	33.0	41.0
26	37.39876133666078	39.0	35.0	41.0	33.0	41.0
27	37.36850487958285	39.0	35.0	41.0	33.0	41.0
28	37.272603577008134	39.0	35.0	41.0	32.0	41.0
29	37.24782396980948	39.0	35.0	41.0	32.0	41.0
30	37.08469018199525	39.0	35.0	41.0	32.0	41.0
31	36.93793622050439	39.0	35.0	41.0	31.0	41.0
32	36.779583105078416	39.0	35.0	41.0	31.0	41.0
33	36.52864091951224	39.0	35.0	41.0	30.0	41.0
34	36.25742969748615	39.0	35.0	41.0	30.0	41.0
35	36.03083977519427	39.0	35.0	41.0	28.0	41.0
36	35.82176714955262	39.0	35.0	41.0	27.0	41.0
37	35.6863143425244	39.0	35.0	40.0	26.0	41.0
38	35.5069757238217	39.0	35.0	40.0	25.0	41.0
39	35.454714224846306	39.0	35.0	40.0	25.0	41.0
40	35.32002607178364	39.0	35.0	40.0	24.0	41.0
41	35.19522769695863	38.0	34.0	40.0	24.0	41.0
42	35.12640123156208	38.0	34.0	40.0	24.0	41.0
43	34.990010043216266	38.0	34.0	40.0	23.0	41.0
44	34.82456580842818	38.0	34.0	40.0	23.0	41.0
45	34.668625854687846	38.0	34.0	40.0	23.0	41.0
46	34.56438310304949	38.0	34.0	40.0	22.0	41.0
47	34.38934708949622	38.0	33.0	40.0	22.0	41.0
48	34.31401409093676	38.0	33.0	40.0	21.0	41.0
49	34.197404995232006	38.0	33.0	40.0	20.0	41.0
50	34.03735645302007	37.0	33.0	40.0	20.0	41.0
51	32.2279734006939	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	30.0
10	43.0
11	28.0
12	13.0
13	15.0
14	21.0
15	47.0
16	87.0
17	185.0
18	344.0
19	652.0
20	1185.0
21	1852.0
22	2951.0
23	4671.0
24	7168.0
25	10316.0
26	13223.0
27	14126.0
28	14369.0
29	14936.0
30	17217.0
31	20782.0
32	25978.0
33	35439.0
34	50209.0
35	61807.0
36	75435.0
37	118913.0
38	151117.0
39	145409.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.119108487024974	19.660863919491955	24.297735711242314	14.922291882240755
2	35.82853998011646	22.884837786840343	24.958280073853146	16.32834215919005
3	32.3370006289691	23.725323107513137	28.221818126483655	15.715858137034106
4	26.480993974070245	25.724962971980442	29.639027532615092	18.155015521334224
5	20.92501572422748	38.996591393268005	24.762234463448777	15.316158419055734
6	24.30623186641508	33.89154340901252	28.197344127254652	13.60488059731775
7	67.7445370990322	18.88441678333029	9.920085418061559	3.450960699575953
8	82.43844725789762	4.3737445979670095	10.465234240266195	2.7225739038691743
9	77.07788057702842	6.732378720555116	12.22127538701889	3.9684653153975695
10	37.227108568182274	31.35436321951022	19.078306652869927	12.340221559437579
11	26.13721671028872	26.46463570515552	30.194321017712582	17.203826566843183
12	24.14873597500355	24.527385517479257	33.15174386755128	18.172134639965915
13	22.638195670257875	25.285065027289143	33.97599772759551	18.10074157485747
14	20.32800231298314	26.955130156024914	33.53635339947654	19.180514131515412
15	19.455941729056345	27.182624221397123	35.01671333211597	18.344720717430558
16	23.631738592326577	26.653072818390243	32.307834723152155	17.40735386613103
17	24.035369367175928	25.957022135654434	31.152230811370142	18.855377685799503
18	24.080893541907603	25.182477123785173	32.40890092722219	18.327728407085033
19	23.144667965183515	27.62214174122994	30.323919086168765	18.909271207417778
20	24.700732444660865	26.929134457361982	30.680503986852514	17.68962911112464
21	23.69045082881896	27.19758759916408	31.81683303913811	17.295128532878852
22	23.913506604175545	24.20326353805263	31.60785298354536	20.27537687422647
23	21.42502079655893	27.16347617018686	32.14995840688214	19.261544626372064
24	20.91791446020249	26.266941587031063	33.3460141619494	19.469129790817053
25	21.592914967435632	27.54580315296123	31.08058412968937	19.78069774991377
26	21.324715442205854	28.07801752997748	30.787022947227467	19.8102440805892
27	20.891538336681073	27.18541400369266	31.70270558159352	20.220342078032747
28	19.85855803761641	27.75871426542496	31.828245784892566	20.554481912066063
29	20.696507192565992	27.196953557733277	31.537601193012353	20.568938056688378
30	21.96446324588634	26.256543307565888	31.553705845354756	20.225287601193013
31	22.89751861545641	26.94802889199992	30.330766733621438	19.82368575892223
32	22.246358066021465	26.723070992350923	30.652732972183333	20.377837969444276
33	21.86567959096719	27.207985878629255	29.84737354677704	21.078960983626512
34	20.582506543307566	27.571038001907194	31.470900034491855	20.375555420293384
35	21.097982226550613	27.860921744070446	30.662370401931543	20.3787256274474
36	21.663293566254794	29.399993913202266	29.099838699860005	19.83687382068294
37	21.714270497291373	28.600594477245522	28.958954693935517	20.726180331527583
38	21.017078539980115	29.504103516140155	29.471006553452227	20.007811390427495
39	21.755483190293585	27.5800413902246	28.897579483433766	21.766895936048044
40	23.288722178262017	26.649648994663906	29.941845719966725	20.11978310710735
41	21.005412177653337	27.55404569156167	29.310720879745165	22.12982125103983
42	21.458751800677664	27.647122973603587	29.462129973420982	21.431995252297767
43	21.72073771988557	27.565585245602286	29.211937224826016	21.501739809686125
44	21.99565808428186	27.506365775965264	28.961998092803377	21.5359780469495
45	21.76955891005742	26.864842656278533	28.61454338872319	22.751055044940856
46	21.81064479477347	27.52094872887374	28.78129628502445	21.887110191328343
47	20.629425609187006	28.17084119544708	30.169973826769734	21.02975936859618
48	21.12435835007203	27.54871974354292	30.225896280966584	21.10102562541847
49	22.086706433745206	26.582567411284924	29.8504169456449	21.480309209324975
50	20.344106965325544	27.86941789924321	29.89594112037657	21.89053401505468
51	20.701199099153936	26.49823990098809	29.479629516911153	23.320931482946822
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	349.0
1	733.0
2	1117.0
3	7890.0
4	14663.0
5	11380.5
6	8098.0
7	6525.5
8	4953.0
9	4709.5
10	4466.0
11	4294.5
12	4123.0
13	3938.5
14	3754.0
15	3671.5
16	3589.0
17	3533.0
18	3477.0
19	3542.5
20	3608.0
21	3753.5
22	3899.0
23	4387.0
24	4875.0
25	6014.5
26	8552.5
27	9951.0
28	11084.0
29	12217.0
30	14754.0
31	17291.0
32	20542.0
33	23793.0
34	26332.5
35	28872.0
36	31432.0
37	33992.0
38	35747.5
39	37503.0
40	39927.0
41	42351.0
42	45701.5
43	49052.0
44	50328.0
45	51604.0
46	54572.5
47	57541.0
48	59278.0
49	61015.0
50	59460.0
51	57905.0
52	54366.5
53	50828.0
54	46341.0
55	41854.0
56	37551.0
57	33248.0
58	30880.5
59	28513.0
60	25665.5
61	22818.0
62	20618.5
63	18419.0
64	15871.5
65	13324.0
66	11331.0
67	9338.0
68	7952.5
69	6567.0
70	5718.0
71	4869.0
72	3931.0
73	2993.0
74	2504.0
75	1548.0
76	1081.0
77	891.5
78	702.0
79	546.0
80	390.0
81	293.5
82	197.0
83	155.5
84	114.0
85	84.0
86	54.0
87	41.5
88	29.0
89	22.0
90	15.0
91	10.5
92	6.0
93	3.0
94	0.0
95	2.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	788592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.892178692573765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.10790167379943	28.822177544290938
2	8.442005223112403	6.566559513218573
3	3.9858522019807747	4.650554282448747
4	2.5334688487027766	3.9412849270327004
5	1.7243311325764246	3.3531497266665204
6	1.3323323896244101	3.109038562510582
7	1.039690705556822	2.8305085693866814
8	0.7853724002784576	2.443587498547627
9	0.6912040370352421	2.4194187829262708
>10	5.290997062775732	38.19022504328771
>50	0.04822104110118649	1.18151529779037
>100	0.016960490290528697	0.9794865027037816
>500	6.651172662946844E-4	0.18857669628169546
>1k	6.651172662946844E-4	0.4551449891737218
>5k	3.325586331473422E-4	0.8687720637339839
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6717	0.851771258141092	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2137	0.2709893075253109	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1382	0.1752490514740195	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	865	0.10968916752896302	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2680828616065088E-4	0.0	0.0	0.04894799845801124	0.0
2	1.2680828616065088E-4	0.0	0.0	0.1657384300119707	0.0
3	1.2680828616065088E-4	0.0	0.0	0.26376123521415384	0.0
4	1.2680828616065088E-4	0.0	0.0	0.3750989104632053	0.0
5	1.2680828616065088E-4	0.0	0.0	0.6999817396067929	0.0
6	1.2680828616065088E-4	0.0	0.0	1.0629070545985757	0.0
7	1.2680828616065088E-4	0.0	0.0	1.3595116359283381	0.0
8	1.2680828616065088E-4	0.0	0.0	1.9227940430539494	0.0
9	1.2680828616065088E-4	0.0	0.0	2.2259926552640654	0.0
10	1.2680828616065088E-4	0.0	0.0	2.7646742548745107	0.0
11	1.2680828616065088E-4	0.0	0.0	3.2684835757907766	0.0
12	1.2680828616065088E-4	0.0	0.0	3.696207925010652	0.0
13	1.2680828616065088E-4	0.0	0.0	3.913810944062329	0.0
14	1.2680828616065088E-4	0.0	0.0	4.028445634751558	0.0
15	1.2680828616065088E-4	0.0	0.0	4.167934749528273	0.0
16	1.2680828616065088E-4	0.0	0.0	4.4283989693022505	0.0
17	1.2680828616065088E-4	0.0	0.0	4.747702233854769	0.0
18	1.2680828616065088E-4	0.0	0.0	5.106696491975572	0.0
19	1.2680828616065088E-4	0.0	0.0	5.335712256781707	0.0
20	1.2680828616065088E-4	0.0	0.0	5.56815184531418	0.0
21	1.2680828616065088E-4	0.0	0.0	5.850807515166271	0.0
22	1.2680828616065088E-4	0.0	0.0	6.161741432832187	0.0
23	1.2680828616065088E-4	0.0	0.0	6.476860023941405	0.0
24	1.2680828616065088E-4	0.0	0.0	6.726799155964048	0.0
25	1.2680828616065088E-4	0.0	0.0	6.957463428490271	0.0
26	1.2680828616065088E-4	0.0	0.0	7.187747276158014	0.0
27	1.2680828616065088E-4	0.0	0.0	7.42855621157709	0.0
28	1.2680828616065088E-4	0.0	0.0	7.679636618175178	0.0
29	1.2680828616065088E-4	0.0	0.0	7.944031894820135	0.0
30	1.2680828616065088E-4	0.0	0.0	8.261940268224887	0.0
31	2.5361657232130175E-4	0.0	0.0	8.534831700042607	0.0
32	2.5361657232130175E-4	0.0	0.0	8.822306084768803	0.0
33	3.8042485848195266E-4	0.0	0.0	9.100777081177592	0.0
34	3.8042485848195266E-4	0.0	0.0	9.38888550733459	0.0
35	3.8042485848195266E-4	0.0	0.0	9.691703694686225	0.0
36	3.8042485848195266E-4	0.0	0.0	9.977909996550814	0.0
37	3.8042485848195266E-4	0.0	0.0	10.266652464138616	0.0
38	3.8042485848195266E-4	0.0	0.0	10.592169334713008	0.0
39	3.8042485848195266E-4	0.0	0.0	11.018118367926634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGACC	20	7.031939E-4	45.0	15
CGCATAG	45	3.8380676E-10	45.0	2
ACTTACG	25	3.8896884E-5	45.0	1
CGTTCGG	20	7.031939E-4	45.0	45
CGTTAGT	25	3.8896884E-5	45.0	38
TATTACG	20	7.031939E-4	45.0	1
CGGTCGT	30	2.164632E-6	44.999996	31
CGTTTTT	3140	0.0	42.0621	1
CGTTATT	670	0.0	41.641792	1
CGGTTTT	740	0.0	40.743244	1
CGTTTTA	420	0.0	40.714287	1
CGGTTTA	100	0.0	40.5	1
GCGCATA	45	1.9270374E-8	40.0	1
CGAATAT	90	0.0	40.0	14
TACGGGA	425	0.0	38.647057	4
CACGACG	35	6.245793E-6	38.57143	26
ACGGGCG	140	0.0	38.57143	5
GCGATCT	70	0.0	38.57143	9
CTACGAA	95	0.0	37.894737	11
ACGGGAG	435	0.0	37.75862	5
>>END_MODULE