##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936077.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240847
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.844876622918285	31.0	31.0	34.0	30.0	34.0
2	31.98175190058419	33.0	31.0	34.0	30.0	34.0
3	31.92033946862531	33.0	31.0	34.0	30.0	34.0
4	35.66714553222585	37.0	35.0	37.0	33.0	37.0
5	31.0120034710833	37.0	35.0	37.0	0.0	37.0
6	33.29191561447724	37.0	35.0	37.0	17.0	37.0
7	29.660410966298105	35.0	32.0	37.0	0.0	37.0
8	32.59892379809589	35.0	33.0	37.0	17.0	37.0
9	36.39730202161538	39.0	35.0	39.0	32.0	39.0
10	36.8164934585027	38.0	35.0	39.0	33.0	39.0
11	36.886953127919384	39.0	37.0	39.0	33.0	39.0
12	36.712738792677506	39.0	35.0	39.0	33.0	39.0
13	36.316902431834315	39.0	35.0	39.0	32.0	39.0
14	37.59444792752245	39.0	36.0	41.0	33.0	41.0
15	37.70589212238475	40.0	36.0	41.0	33.0	41.0
16	37.82020951060217	40.0	36.0	41.0	33.0	41.0
17	37.763376749554695	40.0	36.0	41.0	33.0	41.0
18	37.67549107939895	39.0	36.0	41.0	33.0	41.0
19	37.55648191590512	39.0	36.0	41.0	33.0	41.0
20	37.508700544328974	39.0	35.0	41.0	33.0	41.0
21	37.42282029670289	39.0	35.0	41.0	33.0	41.0
22	37.414279604894396	39.0	35.0	41.0	33.0	41.0
23	37.38248763737975	39.0	35.0	41.0	33.0	41.0
24	37.350749646040846	39.0	35.0	41.0	33.0	41.0
25	37.22919945027341	39.0	35.0	41.0	32.0	41.0
26	36.980024662960304	39.0	35.0	41.0	32.0	41.0
27	36.999534974485876	39.0	35.0	40.0	32.0	41.0
28	36.8945139445374	39.0	35.0	40.0	31.0	41.0
29	36.85084306634502	39.0	35.0	40.0	31.0	41.0
30	36.79906745776364	39.0	35.0	40.0	31.0	41.0
31	36.63352667876286	39.0	35.0	40.0	31.0	41.0
32	36.428085049844924	38.0	35.0	40.0	30.0	41.0
33	36.36610379203395	38.0	35.0	40.0	30.0	41.0
34	36.19945442542361	38.0	35.0	40.0	30.0	41.0
35	36.130065975494816	38.0	35.0	40.0	30.0	41.0
36	36.03369774171985	38.0	35.0	40.0	30.0	41.0
37	35.874733752133096	38.0	35.0	40.0	29.0	41.0
38	35.78544054939443	38.0	35.0	40.0	29.0	41.0
39	35.68530228734425	38.0	35.0	40.0	28.0	41.0
40	35.54748865462306	38.0	34.0	40.0	27.0	41.0
41	35.467437833977584	38.0	34.0	40.0	27.0	41.0
42	35.35916577744377	38.0	34.0	40.0	27.0	41.0
43	35.137265566936684	38.0	34.0	40.0	26.0	41.0
44	34.87455106353826	38.0	34.0	40.0	24.0	41.0
45	34.748383828737744	38.0	34.0	40.0	24.0	41.0
46	34.51842040797685	37.0	33.0	40.0	23.0	41.0
47	34.275544225172	37.0	33.0	40.0	23.0	41.0
48	34.138627427370906	37.0	33.0	40.0	22.0	41.0
49	33.95491743721117	37.0	33.0	40.0	22.0	41.0
50	33.839375205005666	37.0	33.0	40.0	21.0	41.0
51	31.6500267804872	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	6.0
11	3.0
12	7.0
13	7.0
14	7.0
15	17.0
16	27.0
17	54.0
18	139.0
19	227.0
20	385.0
21	557.0
22	840.0
23	1256.0
24	1711.0
25	2252.0
26	2827.0
27	3522.0
28	4160.0
29	4848.0
30	6018.0
31	7595.0
32	9741.0
33	14272.0
34	20510.0
35	24609.0
36	22905.0
37	33588.0
38	41153.0
39	37591.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03210336852857	20.321199765826435	25.747466233750053	9.899230631894937
2	30.689607925363404	27.33685700880642	25.054079976084402	16.91945508974577
3	31.21027042064049	27.17451328021524	25.90939476098934	15.705821538154929
4	27.87786437032639	24.912911516439898	28.68293979165196	18.526284321581752
5	23.047827043724855	38.98117892271857	20.595232658077535	17.375761375479037
6	24.40844187388674	36.65771215751079	24.698667618861766	14.235178349740707
7	67.58689126291794	19.500346693128833	8.725871611437967	4.186890432515248
8	79.89138332634411	8.389973717754424	7.658388935714375	4.06025402018709
9	74.60130290184225	8.218495559421541	9.053050276731701	8.127151262004508
10	44.48799445290994	26.63931873762181	15.678833450281715	13.193853359186539
11	36.64650172100961	23.835464008270808	22.976827612550707	16.54120665816888
12	32.59496692921232	23.967913239525508	24.660053893135476	18.777065938126693
13	23.991994917935454	29.35971799524179	28.790061740441026	17.85822534638173
14	19.320979709109935	32.12122218669944	27.756625575572873	20.801172528617755
15	18.0874164926281	25.71798693776547	38.749496568360826	17.445100001245603
16	21.670604159487144	24.925367556996765	32.930449621543964	20.473578661972123
17	20.81653497863789	24.81326319198495	28.548414553637784	25.82178727573937
18	22.6562921688873	25.987452615145717	29.934356666265305	21.42189854970168
19	26.276847957416948	28.031073669175864	25.705115695856705	19.98696267755048
20	27.622515538910598	25.987452615145717	28.20545823697202	18.18457360897167
21	24.425049927962565	29.194052655835446	26.77799598915494	19.602901427047044
22	21.308963781986073	26.769691962117026	27.031684015163155	24.889660240733743
23	20.994241157249206	30.053934655611236	27.896548431161694	21.055275755977863
24	23.418601850967626	25.284516726386464	28.77926650529174	22.51761491735417
25	20.712319439312093	29.20609349504042	27.79482409994727	22.286762965700216
26	19.845794217905972	30.08715076376289	27.061578512499633	23.005476505831503
27	21.629914427001374	29.792357803917007	27.992044742097683	20.585683026983936
28	19.166940007556665	28.06719618679078	32.225437726025234	20.540426079627316
29	20.704845814977972	28.65387569701927	29.708902332185993	20.932376155816762
30	22.28344135488505	28.810821808035808	28.350778710135483	20.554958126943664
31	26.483203029309067	27.67773731871271	24.85810493798968	20.98095471398855
32	26.00406066922154	30.276897781579176	25.638268278201515	18.08077327099777
33	24.569955199774128	28.97690234879405	24.858520139341575	21.594622312090248
34	22.545433407931174	29.16623416525844	27.63248037135609	20.655852055454293
35	22.259359676475107	28.81206741209149	27.509995972546886	21.418576938886513
36	25.12383380320286	29.72634078896561	24.843572890673332	20.306252517158196
37	23.618728902581307	29.99414566093827	27.474288656283864	18.912836780196557
38	22.294651791386233	30.989383301432028	24.470306875319185	22.245658031862554
39	23.11425926002815	27.700988594418863	27.789426482372626	21.39532566318036
40	24.37522576573509	29.143813292256084	26.53261199018464	19.948348951824187
41	21.252496398128272	27.05742649898068	26.165158793757033	25.524918309134016
42	22.17964101691115	26.94781334208024	27.55982013477436	23.312725506234248
43	23.371268896851525	25.201061254655443	25.90482754611849	25.52284230237454
44	22.3262070941303	26.824498540567248	27.198179757273287	23.651114608029165
45	21.136240019597505	28.384410019639024	25.530315926708656	24.949034034054815
46	24.06133354370202	29.512512092739374	25.179055583004978	21.24709878055363
47	21.7690068798864	26.88096592442505	29.135509265218168	22.214517930470382
48	23.3550760441276	25.24216618849311	28.53678891578471	22.86596885159458
49	20.900820853072695	24.815339198744432	31.038792262307606	23.245047685875264
50	20.20369778323998	26.85646904466321	28.738161571454075	24.201671600642733
51	20.060868518187895	27.412838856203315	25.66982358094558	26.85646904466321
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	435.5
2	683.0
3	832.0
4	981.0
5	793.0
6	605.0
7	553.0
8	501.0
9	555.5
10	610.0
11	600.0
12	590.0
13	604.0
14	618.0
15	599.5
16	581.0
17	573.0
18	565.0
19	705.5
20	846.0
21	886.0
22	926.0
23	1005.0
24	1084.0
25	1648.0
26	2473.5
27	2735.0
28	3007.0
29	3279.0
30	3710.5
31	4142.0
32	4998.5
33	5855.0
34	6334.5
35	6814.0
36	7028.0
37	7242.0
38	8281.0
39	9320.0
40	10643.0
41	11966.0
42	13930.5
43	15895.0
44	18686.5
45	21478.0
46	23793.0
47	26108.0
48	23569.0
49	21030.0
50	20092.0
51	19154.0
52	17443.5
53	15733.0
54	13801.5
55	11870.0
56	10987.0
57	10104.0
58	9905.0
59	9706.0
60	9156.5
61	8607.0
62	7647.5
63	6688.0
64	5685.0
65	4682.0
66	3678.5
67	2675.0
68	2204.5
69	1734.0
70	1425.0
71	1116.0
72	1013.5
73	911.0
74	698.0
75	353.5
76	222.0
77	180.5
78	139.0
79	103.5
80	68.0
81	54.5
82	41.0
83	33.0
84	25.0
85	22.5
86	20.0
87	14.5
88	9.0
89	5.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	240847.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.997566920077894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.71808208400716	28.391053241269354
2	8.87276541806897	6.742869954784572
3	3.838673018925652	4.375807047627747
4	2.353686787009922	3.5773748479325045
5	1.606276498098693	3.051729936432673
6	1.2238297128370994	2.790153084738444
7	0.8785349010009179	2.336753208468447
8	0.6960531491761003	2.1158660892599865
9	0.5813191135976223	1.987984072876141
>10	5.119323397001617	39.214522082483896
>50	0.07539665195157132	1.7816290009840272
>100	0.03278115302242231	2.152819009578695
>500	0.0010927051007474104	0.26988087873214117
>1k	0.002185410201494821	1.211557544831366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC	1461	0.6066091751194742	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG	1457	0.6049483697118918	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC	650	0.26988087873214117	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	331	0.13743164747744419	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	325	0.13494043936607059	TruSeq Adapter, Index 15 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG	291	0.12082359340162012	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCT	271	0.11251956636370809	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC	267	0.11085876095612568	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1520135189560177E-4	0.0	0.0	0.1424140637001914	0.0
2	4.1520135189560177E-4	0.0	0.0	0.6385796792154356	0.0
3	4.1520135189560177E-4	0.0	0.0	0.7946953875281818	0.0
4	4.1520135189560177E-4	0.0	0.0	1.0562722392224109	0.0
5	4.1520135189560177E-4	0.0	0.0	2.2047191785656453	0.0
6	4.1520135189560177E-4	0.0	0.0	2.6153533155903954	0.0
7	4.1520135189560177E-4	0.0	0.0	3.213243262320062	0.0
8	4.1520135189560177E-4	0.0	0.0	4.024961905275964	0.0
9	4.1520135189560177E-4	0.0	0.0	4.29525798536	0.0
10	4.1520135189560177E-4	0.0	0.0	6.108857490439989	0.0
11	4.1520135189560177E-4	0.0	0.0	7.199591441869734	0.0
12	4.1520135189560177E-4	0.0	0.0	8.990770073947361	0.0
13	4.1520135189560177E-4	0.0	0.0	9.416766660992248	0.0
14	4.1520135189560177E-4	0.0	0.0	9.620215323421093	0.0
15	4.1520135189560177E-4	0.0	0.0	10.016317413129498	0.0
16	4.1520135189560177E-4	0.0	0.0	10.627909004471718	0.0
17	4.1520135189560177E-4	0.0	0.0	11.355341772992812	0.0
18	4.1520135189560177E-4	0.0	0.0	12.135089911852752	0.0
19	4.1520135189560177E-4	0.0	0.0	12.592641801641706	0.0
20	4.1520135189560177E-4	0.0	0.0	13.058912919820466	0.0
21	4.1520135189560177E-4	0.0	0.0	13.602411489451809	0.0
22	4.1520135189560177E-4	0.0	0.0	14.152553280713482	0.0
23	4.1520135189560177E-4	0.0	0.0	14.733835173367325	0.0
24	4.1520135189560177E-4	0.0	0.0	15.166890183394438	0.0
25	4.1520135189560177E-4	0.0	0.0	15.55178183660166	0.0
26	4.1520135189560177E-4	0.0	0.0	15.925047851955807	0.0
27	4.1520135189560177E-4	0.0	0.0	16.29831386730995	0.0
28	4.1520135189560177E-4	0.0	0.0	16.64666780155036	0.0
29	4.1520135189560177E-4	0.0	0.0	17.022840226367776	0.0
30	4.1520135189560177E-4	0.0	0.0	17.497000170232553	0.0
31	4.1520135189560177E-4	0.0	0.0	17.95247605326203	0.0
32	4.1520135189560177E-4	0.0	0.0	18.365186197046256	0.0
33	8.304027037912035E-4	0.0	0.0	18.77457473001532	0.0
34	8.304027037912035E-4	0.0	0.0	19.176074437298368	0.0
35	8.304027037912035E-4	0.0	0.0	19.622831091938036	0.0
36	8.304027037912035E-4	0.0	0.0	20.06626613576254	0.0
37	8.304027037912035E-4	0.0	0.0	20.514268394457893	0.0
38	8.304027037912035E-4	0.0	0.0	20.960609847745665	0.0
39	8.304027037912035E-4	0.0	0.0	21.434354590258547	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGAT	20	7.022169E-4	45.000004	21
GATGTTA	20	7.022169E-4	45.000004	9
GTATTAG	20	7.022169E-4	45.000004	1
CATGCAA	20	7.022169E-4	45.000004	37
TCCGACA	20	7.022169E-4	45.000004	21
TCCTGAC	20	7.022169E-4	45.000004	20
GCCGGAT	40	6.7757355E-9	45.000004	19
AAAGTAG	20	7.022169E-4	45.000004	39
CTAAATG	20	7.022169E-4	45.000004	1
AATTCGG	20	7.022169E-4	45.000004	9
CCGACAC	20	7.022169E-4	45.000004	22
TTCGGTA	20	7.022169E-4	45.000004	35
AACTCAA	20	7.022169E-4	45.000004	44
CGGTACT	20	7.022169E-4	45.000004	37
CTTACGG	20	7.022169E-4	45.000004	2
GCTCACT	20	7.022169E-4	45.000004	39
TAATACG	55	1.8189894E-12	45.0	4
GGTGTCG	25	3.881595E-5	45.0	14
CGGCATC	25	3.881595E-5	45.0	36
TAGCGCG	25	3.881595E-5	45.0	1
>>END_MODULE