##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936076.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	170437
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.613587425265642	31.0	31.0	34.0	30.0	34.0
2	31.74435715249623	31.0	31.0	34.0	30.0	34.0
3	31.69367566901553	31.0	31.0	34.0	30.0	34.0
4	35.474544846482864	37.0	35.0	37.0	33.0	37.0
5	30.914865903530337	35.0	35.0	37.0	0.0	37.0
6	33.14090250356437	36.0	35.0	37.0	17.0	37.0
7	29.651167293486743	35.0	32.0	37.0	0.0	37.0
8	32.53440274118883	35.0	33.0	37.0	17.0	37.0
9	36.23812317747907	39.0	35.0	39.0	32.0	39.0
10	36.59799221999918	38.0	35.0	39.0	32.0	39.0
11	36.67028286111584	38.0	35.0	39.0	33.0	39.0
12	36.51353285964902	38.0	35.0	39.0	33.0	39.0
13	35.99107588141073	38.0	35.0	39.0	31.0	39.0
14	37.13874334798195	39.0	35.0	40.0	32.0	41.0
15	37.303549111988595	39.0	35.0	41.0	32.0	41.0
16	37.484337321121586	39.0	35.0	41.0	33.0	41.0
17	37.44153558206258	39.0	35.0	41.0	33.0	41.0
18	37.32362691199681	39.0	36.0	40.0	32.0	41.0
19	37.21250667402031	39.0	35.0	40.0	32.0	41.0
20	37.191220216267595	39.0	35.0	40.0	32.0	41.0
21	37.095366616403716	39.0	35.0	40.0	32.0	41.0
22	37.11858340618528	39.0	35.0	40.0	32.0	41.0
23	37.10473078028832	39.0	35.0	40.0	32.0	41.0
24	36.999178582115384	39.0	35.0	40.0	32.0	41.0
25	36.93040830336136	39.0	35.0	40.0	32.0	41.0
26	36.67479479221061	38.0	35.0	40.0	31.0	41.0
27	36.64349877080681	38.0	35.0	40.0	31.0	41.0
28	36.572569336470366	38.0	35.0	40.0	31.0	41.0
29	36.54525132453634	38.0	35.0	40.0	31.0	41.0
30	36.46519241713947	38.0	35.0	40.0	31.0	41.0
31	36.33467498254487	38.0	35.0	40.0	30.0	41.0
32	36.207231997746966	38.0	35.0	40.0	30.0	41.0
33	36.05074015618674	38.0	35.0	40.0	30.0	41.0
34	35.90200484636552	38.0	35.0	40.0	30.0	41.0
35	35.827889484090896	38.0	35.0	40.0	29.0	41.0
36	35.590000997436	38.0	35.0	40.0	28.0	41.0
37	35.57895292688794	38.0	34.0	40.0	28.0	41.0
38	35.4643005920076	38.0	34.0	40.0	27.0	41.0
39	35.48808063976718	38.0	34.0	40.0	28.0	41.0
40	35.30769140503529	38.0	34.0	40.0	27.0	41.0
41	35.18727154315084	38.0	34.0	40.0	26.0	41.0
42	35.16575039457395	38.0	34.0	40.0	26.0	41.0
43	34.959914807230824	38.0	34.0	40.0	26.0	41.0
44	34.75121012456216	37.0	34.0	40.0	24.0	41.0
45	34.64822779091394	37.0	34.0	40.0	24.0	41.0
46	34.4752606534966	37.0	33.0	40.0	23.0	41.0
47	34.270592653003746	37.0	33.0	40.0	23.0	41.0
48	34.172967137417345	37.0	33.0	40.0	23.0	41.0
49	33.971848835640145	37.0	33.0	40.0	22.0	41.0
50	33.73106191730669	36.0	33.0	40.0	22.0	41.0
51	31.2822919905889	35.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	7.0
11	8.0
12	2.0
13	3.0
14	7.0
15	11.0
16	20.0
17	48.0
18	105.0
19	162.0
20	290.0
21	422.0
22	679.0
23	1049.0
24	1314.0
25	1699.0
26	2065.0
27	2560.0
28	3009.0
29	3721.0
30	4651.0
31	5896.0
32	7763.0
33	11131.0
34	15645.0
35	17900.0
36	16930.0
37	23520.0
38	27399.0
39	22414.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.696128188128164	20.301929745301784	26.101726737738872	10.90021532883118
2	29.32637866191027	29.402066452706865	24.400218262466485	16.871336622916385
3	27.92527444158252	29.284721040619115	25.72094087551412	17.069063642284245
4	25.933922798453384	25.326660290899277	30.654141999683166	18.08527491096417
5	22.65059816823812	39.358824668352526	21.338676461097062	16.651900702312293
6	24.97227714639427	36.95500390173495	24.62728163485628	13.445437317014497
7	65.59021808644836	19.0698029183804	10.5235365560295	4.816442439141736
8	76.53326449069158	7.953085304247318	9.889871330755645	5.623778874305462
9	70.9411688776498	7.865662972241942	10.91018968885864	10.282978461249611
10	42.764188527139055	26.409171717408782	17.131843437751193	13.69479631770097
11	31.96489025270334	23.777114124280526	26.794651396117043	17.46334422689909
12	31.3828570087481	20.733174134724266	30.17243908306295	17.711529773464683
13	21.36683935999812	29.16972253677312	32.744650516026454	16.71878758720231
14	17.189342689674188	34.09353602797515	28.530190040894876	20.186931241455788
15	14.393588246683525	26.0037433186456	42.611052764364544	16.99161567030633
16	16.1115250796482	26.393330086776935	35.041686957644174	22.453457875930695
17	16.98340149146019	24.373815544746737	30.493965512183387	28.148817451609688
18	18.548789288710786	25.98086096328849	32.87373046932298	22.59661927867775
19	21.94300533334898	28.17404671520855	28.294912489658934	21.588035461783534
20	23.471429325791934	29.326965388970706	29.198472162734618	18.003133122502742
21	20.86284081508123	29.54992167193743	29.9870333319643	19.600204181017034
22	18.284762111513345	25.367731185129987	28.718529427295714	27.62897727606095
23	17.123629258905048	30.331442116441853	29.31405739364105	23.230871231012046
24	20.371163538433557	24.092773282796575	31.098294384435306	24.437768794334563
25	17.362427172503622	30.56319930531516	28.742585236773703	23.331788285407512
26	16.6384059799222	30.796716675369783	29.538187130728655	23.026690213979357
27	19.39778334516566	31.057223490204596	27.18658507249013	22.358408092139616
28	14.768506838303889	28.57067420806515	33.9110639121787	22.749755041452264
29	17.55311346714622	28.15409799515363	31.41688717825355	22.875901359446598
30	17.654617248602122	28.29315230847762	28.95615388677341	25.096076556146844
31	21.464236052030955	30.80258394597417	24.14733889941738	23.585841102577493
32	23.688518338154275	29.125718007240213	25.8523677370524	21.333395917553112
33	19.345564636786612	27.319772115209723	26.094099285953163	27.240563962050494
34	18.158029066458575	28.799497761636268	28.063742027846068	24.978731144059097
35	19.68175924241802	25.552550209168196	27.17719743952311	27.588493108890678
36	21.130388354641305	27.631324184302702	27.623696732517	23.614590728538992
37	18.741235764534697	26.70546888293035	31.348826839242655	23.2044685132923
38	17.907496611651226	29.047683308201854	26.523583494194337	26.52123658595258
39	19.866578266456226	24.12739017936246	31.206252163556037	24.799779390625275
40	23.23497832043512	22.874141178265283	29.004265505729386	24.886614995570213
41	17.544899288300076	25.20638124350933	28.56715384570252	28.681565622488076
42	21.51469457922869	23.990096047219794	31.2602310531164	23.23497832043512
43	21.489465315629822	24.163180530049228	29.430816078668364	24.916538075652586
44	19.100899452583654	26.52417022125477	29.412040812734325	24.96288951342725
45	18.784653567007165	25.486250051338615	27.595533833615942	28.133562548038277
46	22.839524281699397	27.259339227984537	26.57932256493602	23.321813925380052
47	16.9775342208558	24.76457576699895	33.769662690612954	24.488227321532296
48	20.726133409999004	23.09651073417157	29.774051409025038	26.403304446804395
49	19.063348920715573	21.140362714668765	34.27014087316721	25.52614749144845
50	18.561110556979997	22.645904351754606	31.896243186632013	26.89674190463338
51	17.944460416458867	23.741323773593763	27.404847538973343	30.909368270974024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	505.0
1	519.5
2	534.0
3	620.0
4	706.0
5	579.5
6	453.0
7	432.5
8	412.0
9	438.5
10	465.0
11	483.5
12	502.0
13	560.5
14	619.0
15	563.5
16	508.0
17	520.5
18	533.0
19	539.0
20	545.0
21	611.0
22	677.0
23	785.0
24	893.0
25	1076.5
26	1436.0
27	1612.0
28	1930.5
29	2249.0
30	2619.0
31	2989.0
32	3115.0
33	3241.0
34	3623.5
35	4006.0
36	4991.0
37	5976.0
38	6420.5
39	6865.0
40	7710.0
41	8555.0
42	10146.0
43	11737.0
44	14564.5
45	17392.0
46	20718.0
47	24044.0
48	21040.5
49	18037.0
50	16870.0
51	15703.0
52	13235.0
53	10767.0
54	8910.5
55	7054.0
56	6001.0
57	4948.0
58	4516.5
59	4085.0
60	3894.0
61	3703.0
62	3494.5
63	3286.0
64	2767.5
65	2249.0
66	1777.0
67	1305.0
68	1005.0
69	705.0
70	569.5
71	434.0
72	355.0
73	276.0
74	262.0
75	188.5
76	129.0
77	100.0
78	71.0
79	67.0
80	63.0
81	59.0
82	55.0
83	37.5
84	20.0
85	14.0
86	8.0
87	5.5
88	3.0
89	5.5
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	170437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.281312156397966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.01491750274066	31.71729143319819
2	10.241039090795553	8.660091412076017
3	4.168574719342797	5.287584268674055
4	2.3104783314599726	3.9076022225221054
5	1.473710503309604	3.1155206909297863
6	1.0060641383234115	2.552262712908582
7	0.7410182756754506	2.193185751920064
8	0.5703342908288581	1.9291585747226245
9	0.44266822086230107	1.6844933905196642
>10	3.9382207235335747	31.101228019737498
>50	0.05689466161553086	1.5213832677176904
>100	0.03052884281808973	3.0767967049408287
>500	0.001387674673549533	0.40836203406537314
>1k	0.0041630240206485996	2.8450395160675206
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG	1909	1.1200619583775824	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC	1582	0.9282022096141096	No Hit
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	1358	0.7967753480758286	No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	696	0.40836203406537314	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT	415	0.24349173008208314	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG	370	0.21708901236233916	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	352	0.20652792527444158	No Hit
TGCATGGGAGGGAAAATCTGTTTCTTGTTCTGTTACAACTTTCTGAATGTT	312	0.1830588428568914	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTC	294	0.17249775576899382	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG	292	0.17132430164811632	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC	291	0.17073757458767755	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC	254	0.14902867335144365	No Hit
GAATATGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	248	0.14550831098881112	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	230	0.13494722390091354	No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT	228	0.13377376978003602	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT	226	0.13260031565915853	No Hit
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTT	222	0.1302534074174035	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	220	0.129079953296526	No Hit
GAATCTATCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	197	0.11558523090643463	No Hit
CCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	190	0.11147814148336337	No Hit
TCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	181	0.10619759793941456	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2229562829667267	0.0
2	0.0	0.0	0.0	1.2861057164817498	0.0
3	0.0	0.0	0.0	1.6592641269207977	0.0
4	0.0	0.0	0.0	2.279434629804561	0.0
5	0.0	0.0	0.0	4.576471071422285	0.0
6	0.0	0.0	0.0	5.413730586668388	0.0
7	0.0	0.0	0.0	6.637056507683191	0.0
8	0.0	0.0	0.0	8.414252773752178	0.0
9	0.0	0.0	0.0	9.094269436800694	0.0
10	0.0	0.0	0.0	12.056654364955966	0.0
11	0.0	0.0	0.0	13.870814435832594	0.0
12	0.0	0.0	0.0	16.505218937202603	0.0
13	0.0	0.0	0.0	17.16117979077313	0.0
14	0.0	0.0	0.0	17.46275749983865	0.0
15	0.0	0.0	0.0	17.997265851898355	0.0
16	0.0	0.0	0.0	18.963018593380546	0.0
17	0.0	0.0	0.0	20.104202725933924	0.0
18	0.0	0.0	0.0	21.572780558212127	0.0
19	0.0	0.0	0.0	22.32437792263417	0.0
20	0.0	0.0	0.0	23.004394585682686	0.0
21	0.0	0.0	0.0	23.786501757247546	0.0
22	0.0	0.0	0.0	24.62786836191672	0.0
23	0.0	0.0	0.0	25.488010232519933	0.0
24	0.0	0.0	0.0	26.136343634304758	0.0
25	0.0	0.0	0.0	26.64914308512823	0.0
26	0.0	0.0	0.0	27.12439200408362	0.0
27	0.0	0.0	0.0	27.601987831280766	0.0
28	0.0	0.0	0.0	28.08134383965923	0.0
29	0.0	0.0	0.0	28.64636199886175	0.0
30	0.0	0.0	0.0	29.28648122180043	0.0
31	0.0	0.0	0.0	29.900197727019368	0.0
32	0.0	0.0	0.0	30.409476815480208	0.0
33	0.0	0.0	0.0	30.90760808979271	0.0
34	0.0	0.0	0.0	31.480253700780935	0.0
35	0.0	0.0	0.0	32.0640471259175	0.0
36	0.0	0.0	0.0	32.614983835669484	0.0
37	0.0	0.0	0.0	33.13541073827866	0.0
38	0.0	0.0	0.0	33.63060837728897	0.0
39	0.0	0.0	0.0	34.18447872234315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTAA	20	7.016364E-4	45.000004	26
TAATACG	50	2.1827873E-11	45.000004	4
ATCTATG	20	7.016364E-4	45.000004	30
TACCAGA	20	7.016364E-4	45.000004	11
CAACGGG	25	3.8767852E-5	45.000004	3
GATCCAT	20	7.016364E-4	45.000004	9
ATAGAGT	20	7.016364E-4	45.000004	14
AAGCGAA	20	7.016364E-4	45.000004	1
TATGATG	20	7.016364E-4	45.000004	33
ACCTCGC	20	7.016364E-4	45.000004	29
CCCAATC	25	3.8767852E-5	45.000004	29
TGCGCGG	40	6.7557266E-9	45.000004	2
TCATGTG	20	7.016364E-4	45.000004	12
GGAACTA	20	7.016364E-4	45.000004	8
TATTAGG	20	7.016364E-4	45.000004	2
GGAGTTA	20	7.016364E-4	45.000004	31
TGTTACG	20	7.016364E-4	45.000004	1
AGCGAAG	25	3.8767852E-5	45.000004	2
GGACGCT	20	7.016364E-4	45.000004	8
CATGTGT	20	7.016364E-4	45.000004	13
>>END_MODULE