##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936075.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	265897
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75206188862605	31.0	31.0	34.0	30.0	34.0
2	31.871585613978343	31.0	31.0	34.0	30.0	34.0
3	31.847109971154243	31.0	31.0	34.0	30.0	34.0
4	35.59328612206983	37.0	35.0	37.0	33.0	37.0
5	30.996479839938022	37.0	35.0	37.0	0.0	37.0
6	33.25252635418978	37.0	35.0	37.0	17.0	37.0
7	29.690553108910592	35.0	32.0	37.0	0.0	37.0
8	32.56263139486342	35.0	33.0	37.0	17.0	37.0
9	36.373840246411206	39.0	35.0	39.0	32.0	39.0
10	36.64624647889973	38.0	35.0	39.0	32.0	39.0
11	36.78785394344427	39.0	35.0	39.0	33.0	39.0
12	36.72851893778418	39.0	35.0	39.0	33.0	39.0
13	36.39147865526877	39.0	35.0	39.0	32.0	39.0
14	37.6630274128704	39.0	36.0	41.0	33.0	41.0
15	37.741381061087566	39.0	36.0	41.0	33.0	41.0
16	37.884233368560004	40.0	36.0	41.0	33.0	41.0
17	37.76872623609894	39.0	36.0	41.0	33.0	41.0
18	37.68214759850619	39.0	36.0	41.0	33.0	41.0
19	37.57944993738177	39.0	36.0	41.0	32.0	41.0
20	37.592244365299344	39.0	36.0	41.0	33.0	41.0
21	37.5521950228848	39.0	36.0	41.0	33.0	41.0
22	37.558486932910114	39.0	36.0	41.0	33.0	41.0
23	37.57930702490062	39.0	36.0	41.0	33.0	41.0
24	37.4964140249796	39.0	35.0	41.0	33.0	41.0
25	37.42376559344408	39.0	35.0	41.0	33.0	41.0
26	37.24451197268115	39.0	35.0	41.0	32.0	41.0
27	37.24668574673652	39.0	35.0	41.0	32.0	41.0
28	37.14352173962098	39.0	35.0	41.0	32.0	41.0
29	37.09344219754266	39.0	35.0	41.0	32.0	41.0
30	36.99670549122405	39.0	35.0	40.0	31.0	41.0
31	36.688315400324186	39.0	35.0	40.0	31.0	41.0
32	36.47007299819103	39.0	35.0	40.0	30.0	41.0
33	36.381534955264634	39.0	35.0	40.0	30.0	41.0
34	36.191265790888956	38.0	35.0	40.0	30.0	41.0
35	36.08426947276577	38.0	35.0	40.0	30.0	41.0
36	35.86991579446177	38.0	35.0	40.0	29.0	41.0
37	35.66546068590469	38.0	35.0	40.0	27.0	41.0
38	35.433581424386134	38.0	34.0	40.0	26.0	41.0
39	35.29481340519073	38.0	34.0	40.0	25.0	41.0
40	35.19929521581665	38.0	34.0	40.0	25.0	41.0
41	35.11291214267179	38.0	34.0	40.0	24.0	41.0
42	35.24958160490716	38.0	34.0	40.0	25.0	41.0
43	35.01858614425887	38.0	34.0	40.0	24.0	41.0
44	34.89233048887351	38.0	34.0	40.0	24.0	41.0
45	34.7051489862616	38.0	34.0	40.0	24.0	41.0
46	34.48333753295449	38.0	33.0	40.0	23.0	41.0
47	34.32800294851014	38.0	33.0	40.0	22.0	41.0
48	34.161573842502925	37.0	33.0	40.0	22.0	41.0
49	34.00590830283907	37.0	33.0	40.0	21.0	41.0
50	33.873702975212204	37.0	33.0	40.0	20.0	41.0
51	31.49553774581887	35.0	28.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	8.0
11	6.0
12	3.0
13	5.0
14	5.0
15	7.0
16	15.0
17	33.0
18	99.0
19	197.0
20	351.0
21	559.0
22	907.0
23	1401.0
24	2131.0
25	2731.0
26	3501.0
27	4106.0
28	4835.0
29	5725.0
30	6966.0
31	8848.0
32	10799.0
33	14791.0
34	20562.0
35	25205.0
36	25827.0
37	38391.0
38	45841.0
39	42035.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96251179968183	20.016773412261138	25.989762953324032	10.030951834733
2	28.180084769666454	29.673144112193818	26.200746905756738	15.946024212382989
3	29.259450087815956	28.27072136955287	26.74682301793552	15.723005524695651
4	26.29589653136365	24.709191905136198	31.42156549340534	17.57334607009481
5	23.86036698420817	38.46489430117677	21.52261966099655	16.152119053618506
6	22.590702414844845	38.036156857730624	25.135296750245395	14.237843977179132
7	67.47311929055235	18.413521025058575	9.944076089613647	4.169283594775421
8	80.13478903485183	7.552924628709613	7.684930631033822	4.6273557054047245
9	75.85343196801769	5.9688526008191145	8.497275260721256	9.680440170441939
10	39.29077800802566	27.054084852405254	18.09422445533421	15.560912684234873
11	30.29631774709756	25.964941311861363	24.671207271988777	19.067533669052303
12	27.5407394592643	22.950616216053586	28.77053896809667	20.738105356585447
13	20.74412272421276	28.4771922962651	32.634065070309184	18.144619909212967
14	17.741456278182905	31.572375769564907	28.387683952808796	22.298483999443395
15	15.287498542668779	27.23460588122468	40.24415469147828	17.233740884628258
16	18.129952575621385	24.892721617769286	33.70101956772735	23.276306238881972
17	18.527474924500844	25.114988134503207	30.897302338875583	25.46023460212037
18	19.996464796518953	27.83295787466575	31.805924850600043	20.36465247821525
19	20.28943538287382	29.945429997329793	27.682899769459603	22.082234850336786
20	22.058541465304234	28.458764107906443	30.517832092878066	18.96486233391125
21	20.438741317126556	30.33956757691888	29.999586305975622	19.22210479997894
22	18.707619867843565	24.916791088278543	29.44297980044905	26.932609243428846
23	17.791475646584956	29.777695874718408	30.069538204643152	22.361290274053484
24	20.91524161611451	25.574564587039344	30.931902202732637	22.578291594113512
25	17.226219175094116	30.920619638431425	29.56407932394875	22.289081862525713
26	17.608698104905283	28.755119463551676	28.0774134345254	25.558768997017644
27	19.42218227358714	30.05487087105157	27.5723306393077	22.950616216053586
28	16.614328104491587	26.57119110031328	33.71192604655186	23.10255474864327
29	19.20217226971346	24.227050323997638	30.802904884222087	25.767872522066813
30	19.664005235109837	28.306825575316758	29.32752155909995	22.701647630473452
31	24.293241367898098	26.86528994309827	25.468508482607927	23.37296020639571
32	23.359045043757547	29.04846613538325	27.507643937314075	20.084844883545134
33	22.11871514157738	26.18532740121175	27.450478944854584	24.24547851235629
34	19.956599735987997	25.857380865523115	29.723539566072578	24.46247983241631
35	18.313858373731183	25.82390925809618	28.468166244824122	27.394066123348516
36	23.334975573248286	24.974332166214737	29.82357830287668	21.867113957660298
37	19.396232375694346	25.83180705310703	33.7984257061945	20.97353486500412
38	21.648608295693446	27.983015979871904	27.9721095010474	22.39626622338725
39	20.757661801374216	25.146579314546607	33.40804898137249	20.68770990270669
40	22.44402907892906	26.863409515714732	29.907821449659078	20.78473995569713
41	18.29430192894241	25.4670041407011	32.220747131408025	24.017946798948465
42	19.692211645862873	25.267678838046308	31.74462291789678	23.29548659819404
43	22.022437259540347	24.844206591274066	29.17595911198697	23.957397037198614
44	21.830633666419704	24.937475789497437	29.13308536764236	24.098805176440504
45	20.042347224677222	26.207140358860766	27.910807568344133	25.83970484811788
46	23.52941176470588	27.22407548787689	26.846109583786205	22.40040316363103
47	18.667002636359193	24.642624775759035	33.65250454123213	23.037868046649642
48	21.07883879848212	24.451197268115095	30.036442682692922	24.433521250709862
49	20.073938404720625	22.68923680974212	33.68973700342614	23.547087782111117
50	19.789241698853317	23.17137839088068	32.133871386288675	24.90550852397733
51	19.11416826816399	23.785902059820156	29.06877475112544	28.031154920890422
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	199.0
1	322.5
2	446.0
3	577.0
4	708.0
5	588.0
6	468.0
7	425.0
8	382.0
9	426.0
10	470.0
11	460.5
12	451.0
13	496.5
14	542.0
15	510.5
16	479.0
17	528.0
18	577.0
19	708.0
20	839.0
21	940.5
22	1042.0
23	1325.5
24	1609.0
25	1689.0
26	2250.5
27	2732.0
28	3350.0
29	3968.0
30	4787.5
31	5607.0
32	6183.5
33	6760.0
34	8056.5
35	9353.0
36	9874.5
37	10396.0
38	10782.5
39	11169.0
40	12801.0
41	14433.0
42	16708.0
43	18983.0
44	22282.5
45	25582.0
46	30182.0
47	34782.0
48	30687.5
49	26593.0
50	25180.5
51	23768.0
52	20876.0
53	17984.0
54	15361.5
55	12739.0
56	10983.5
57	9228.0
58	8286.0
59	7344.0
60	6241.0
61	5138.0
62	4430.0
63	3722.0
64	3083.0
65	2444.0
66	1955.0
67	1466.0
68	1124.5
69	783.0
70	610.0
71	437.0
72	321.5
73	206.0
74	183.5
75	107.5
76	54.0
77	42.5
78	31.0
79	26.5
80	22.0
81	19.5
82	17.0
83	10.5
84	4.0
85	4.0
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	265897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.761926144600025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68999993949325	28.204382614553243
2	9.211999992537311	6.957257267244998
3	3.9399999968081856	4.463459666675851
4	2.293000078364599	3.4635239843506422
5	1.6329999994579247	3.0832612686831014
6	1.1389999990847315	2.580650030648227
7	0.8969999992734158	2.3710713406988297
8	0.6659999994604708	2.011955423354396
9	0.5339999995674038	1.8148381690392672
>10	4.878999996047497	37.57424934122226
>50	0.08399999993195117	1.9605992038393416
>100	0.02699999997812716	2.2449465074778288
>500	0.0029999999975696846	0.6985956331091644
>1k	0.0029999999975696846	2.571209549102865
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	2785	1.0473980526294016	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	2084	0.7837621334576923	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	1940	0.7296058248118633	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	673	0.2531055258239092	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	615	0.2312925681748948	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT	562	0.21136003790941604	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC	426	0.1602124130772442	No Hit
GAATATGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	420	0.15795590021700132	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	359	0.1350146861378654	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	346	0.1301255749406725	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	333	0.1252364637434796	No Hit
TTCTAAGGTTTGATTTTTCTTTCATTTTGTTTTGTTTTGATTTTGCTATCT	303	0.11395389944226524	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTT	300	0.1128256430121438	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	277	0.10417567704787944	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	272	0.1022952496643437	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.76085476707146E-4	0.0	0.0	0.18578622549333013	0.0
2	3.76085476707146E-4	0.0	0.0	1.0451415397691588	0.0
3	3.76085476707146E-4	0.0	0.0	1.3042644332203823	0.0
4	3.76085476707146E-4	0.0	0.0	1.828527587750144	0.0
5	3.76085476707146E-4	0.0	0.0	3.882706461524575	0.0
6	3.76085476707146E-4	0.0	0.0	4.464510693990531	0.0
7	3.76085476707146E-4	0.0	0.0	5.387800539306574	0.0
8	3.76085476707146E-4	0.0	0.0	6.639413005787955	0.0
9	3.76085476707146E-4	0.0	0.0	7.015498482495102	0.0
10	3.76085476707146E-4	0.0	0.0	9.794017984407496	0.0
11	3.76085476707146E-4	0.0	0.0	10.9888415439061	0.0
12	3.76085476707146E-4	0.0	0.0	13.287475977540176	0.0
13	3.76085476707146E-4	0.0	0.0	13.74818068650643	0.0
14	3.76085476707146E-4	0.0	0.0	13.95089075845158	0.0
15	3.76085476707146E-4	0.0	0.0	14.370226064980049	0.0
16	3.76085476707146E-4	0.0	0.0	15.018221341346461	0.0
17	3.76085476707146E-4	0.0	0.0	15.832070312940726	0.0
18	3.76085476707146E-4	0.0	0.0	16.678262635531805	0.0
19	3.76085476707146E-4	0.0	0.0	17.214560525316195	0.0
20	3.76085476707146E-4	0.0	0.0	17.711369440046333	0.0
21	3.76085476707146E-4	0.0	0.0	18.350338664971776	0.0
22	3.76085476707146E-4	0.0	0.0	19.05324242093743	0.0
23	3.76085476707146E-4	0.0	0.0	19.81782419508306	0.0
24	3.76085476707146E-4	0.0	0.0	20.319146135533686	0.0
25	3.76085476707146E-4	0.0	0.0	20.784363870220425	0.0
26	3.76085476707146E-4	0.0	0.0	21.183766646483413	0.0
27	3.76085476707146E-4	0.0	0.0	21.63995832972918	0.0
28	3.76085476707146E-4	0.0	0.0	22.070576200558865	0.0
29	3.76085476707146E-4	0.0	0.0	22.513228806643173	0.0
30	3.76085476707146E-4	0.0	0.0	23.033731106405863	0.0
31	3.76085476707146E-4	0.0	0.0	23.507974892533575	0.0
32	3.76085476707146E-4	0.0	0.0	23.915275463807415	0.0
33	3.76085476707146E-4	0.0	0.0	24.36620195037928	0.0
34	3.76085476707146E-4	0.0	0.0	24.812239325753957	0.0
35	3.76085476707146E-4	0.0	0.0	25.318450377401774	0.0
36	3.76085476707146E-4	0.0	0.0	25.799087616633507	0.0
37	3.76085476707146E-4	0.0	0.0	26.244748906531477	0.0
38	3.76085476707146E-4	0.0	0.0	26.677247204744695	0.0
39	3.76085476707146E-4	0.0	0.0	27.150362734442282	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAG	35	1.2072269E-7	45.000004	6
ACGCATC	25	3.8826904E-5	45.000004	33
TAGGTAG	25	3.8826904E-5	45.000004	1
CGCGCGG	30	2.159186E-6	45.000004	2
GCGCATG	30	2.159186E-6	45.000004	18
GGCCACC	45	3.8198777E-10	45.000004	8
GCCGATT	25	3.8826904E-5	45.000004	9
TGCGCAC	30	2.159186E-6	45.000004	23
GCGATGC	25	3.8826904E-5	45.000004	9
AAGGCGA	35	1.2072269E-7	45.000004	6
ATTTGCG	25	3.8826904E-5	45.000004	1
TACGTAG	25	3.8826904E-5	45.000004	1
ATCCTTA	20	7.0234964E-4	45.0	24
AGCCGTG	20	7.0234964E-4	45.0	31
ATCTCGC	20	7.0234964E-4	45.0	1
ACGCCGA	20	7.0234964E-4	45.0	42
TTCAGCG	20	7.0234964E-4	45.0	1
GCGGTGT	20	7.0234964E-4	45.0	9
GCGCGAG	20	7.0234964E-4	45.0	1
CGGTTCG	20	7.0234964E-4	45.0	29
>>END_MODULE