##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936073.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	962050
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83918091575282	31.0	31.0	34.0	30.0	34.0
2	31.98595914973234	33.0	31.0	34.0	30.0	34.0
3	31.940003118341043	33.0	31.0	34.0	30.0	34.0
4	35.67603762798191	37.0	35.0	37.0	33.0	37.0
5	31.140276492905773	37.0	35.0	37.0	0.0	37.0
6	33.36753391195884	37.0	35.0	37.0	17.0	37.0
7	29.55140585208669	35.0	32.0	37.0	0.0	37.0
8	32.58257678914817	35.0	33.0	37.0	17.0	37.0
9	36.394472220778546	39.0	35.0	39.0	32.0	39.0
10	36.749404916584375	38.0	35.0	39.0	33.0	39.0
11	36.891575281950004	39.0	37.0	39.0	33.0	39.0
12	36.93118860766072	39.0	37.0	39.0	33.0	39.0
13	36.75738371186529	39.0	35.0	39.0	33.0	39.0
14	37.97301283717063	40.0	37.0	41.0	33.0	41.0
15	38.075747622264956	40.0	37.0	41.0	33.0	41.0
16	38.10973753962892	40.0	37.0	41.0	33.0	41.0
17	38.09529026557871	40.0	37.0	41.0	33.0	41.0
18	37.99627046411309	40.0	37.0	41.0	33.0	41.0
19	37.94028376903487	40.0	37.0	41.0	33.0	41.0
20	37.879113351696894	40.0	37.0	41.0	33.0	41.0
21	37.75804791850735	40.0	36.0	41.0	33.0	41.0
22	37.75085286627514	40.0	36.0	41.0	33.0	41.0
23	37.68286887375916	39.0	36.0	41.0	33.0	41.0
24	37.60792162569513	39.0	36.0	41.0	33.0	41.0
25	37.476110389272904	39.0	36.0	41.0	32.0	41.0
26	37.323134972194794	39.0	36.0	41.0	32.0	41.0
27	37.27644820955252	39.0	36.0	41.0	32.0	41.0
28	37.138645600540514	39.0	35.0	41.0	31.0	41.0
29	37.09522685931085	39.0	35.0	41.0	31.0	41.0
30	36.98682189075412	39.0	35.0	41.0	31.0	41.0
31	36.850525440465674	39.0	35.0	41.0	31.0	41.0
32	36.75677147757393	39.0	35.0	41.0	30.0	41.0
33	36.61259497947092	39.0	35.0	40.0	30.0	41.0
34	36.432571072189596	39.0	35.0	40.0	30.0	41.0
35	36.335948235538694	39.0	35.0	40.0	30.0	41.0
36	36.1938974065797	39.0	35.0	40.0	29.0	41.0
37	36.08522114235227	39.0	35.0	40.0	29.0	41.0
38	35.942410477625906	38.0	35.0	40.0	29.0	41.0
39	35.87588067148277	38.0	35.0	40.0	28.0	41.0
40	35.72081596590614	38.0	35.0	40.0	27.0	41.0
41	35.59429551478613	38.0	35.0	40.0	27.0	41.0
42	35.52106439374253	38.0	34.0	40.0	27.0	41.0
43	35.375397328621176	38.0	34.0	40.0	26.0	41.0
44	35.19144639052024	38.0	34.0	40.0	25.0	41.0
45	35.06453406787589	38.0	34.0	40.0	25.0	41.0
46	34.9068717842108	38.0	34.0	40.0	24.0	41.0
47	34.768557767267815	38.0	34.0	40.0	24.0	41.0
48	34.67346083883374	38.0	34.0	40.0	23.0	41.0
49	34.519451171976506	37.0	33.0	40.0	23.0	41.0
50	34.32732914089704	37.0	33.0	40.0	23.0	41.0
51	32.16592588742789	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	24.0
9	61.0
10	71.0
11	49.0
12	44.0
13	33.0
14	45.0
15	78.0
16	134.0
17	256.0
18	447.0
19	801.0
20	1364.0
21	2274.0
22	3366.0
23	4877.0
24	6513.0
25	8649.0
26	10883.0
27	12913.0
28	15218.0
29	18044.0
30	22141.0
31	28072.0
32	35750.0
33	48789.0
34	68153.0
35	82450.0
36	99846.0
37	150350.0
38	178096.0
39	162242.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73338184086066	19.9856556312042	23.586611922457255	10.694350605477885
2	32.01507198170573	23.14079309807183	24.641338807754277	20.202796112468167
3	31.688165895743463	23.939088404968555	27.732446338547895	16.640299360740084
4	27.84709734421288	24.863572579387768	25.22602775323528	22.063302323164077
5	20.879060339899176	40.90203211891274	20.780624707655527	17.43828283353256
6	25.15492957746479	35.85967465308456	24.83789823813731	14.147497531313341
7	67.05555844290836	19.731510836235124	7.0241671430798815	6.188763577776623
8	79.82963463437451	5.419676731978587	10.007068239696482	4.743620393950419
9	73.39369055662388	7.5855724754430645	12.479392962943713	6.541344004989345
10	41.791798763058054	25.609167922665144	18.29260433449405	14.306428979782757
11	29.387453874538743	28.350085754378675	23.711449508861286	18.551010862221297
12	26.11319577984512	23.616236162361623	28.460682916688324	21.80988514110493
13	24.41297229873707	24.30954732082532	29.446182630840394	21.831297749597216
14	21.269060859622684	26.492593940023905	29.103788784366717	23.134556415986694
15	20.862117353567903	25.29255236214334	33.5730991112728	20.272231173015957
16	24.80110181383504	25.096824489371656	30.357153994075155	19.744919702718153
17	23.196507458032325	24.40070682396965	27.541395977340056	24.86138974065797
18	23.384647367600436	26.994335013772673	29.52185437347331	20.09916324515358
19	23.561353360012475	27.336105192037834	27.896782911491087	21.205758536458603
20	25.33766436255912	27.06408190842472	28.028896626994438	19.569357102021726
21	24.556831765500753	28.780936541759782	27.51281118444987	19.14942050828959
22	25.342133984720128	25.149940231796684	26.937581206797983	22.5703445766852
23	22.934462865755417	27.743880255703967	27.37529234447274	21.946364534067875
24	22.638324411413127	27.227067200249465	28.629489111792527	21.505119276544878
25	22.18637284964399	27.98191362195312	26.91346603606881	22.91824749233408
26	21.245153578296346	26.863572579387768	28.380125773088714	23.511148069227172
27	21.701470817525077	25.91206278259966	29.813835039758846	22.572631360116418
28	21.020944857335895	25.87900836754846	29.181539421028013	23.918507354087627
29	21.189543163037264	26.139285899901253	28.500701626734575	24.170469310326904
30	21.248687698144586	26.563172392287303	29.255651993139647	22.93248791642846
31	23.194012785198275	25.373837118652876	26.635829738579076	24.796320357569773
32	22.792058624811602	26.350813367288602	27.6470037939816	23.210124213918196
33	21.916740294163507	24.631879839925162	28.465880151759265	24.98549971415207
34	20.51660516605166	25.066576581258772	29.571539940751517	24.84527831193805
35	21.706875941998856	24.708487084870846	30.090847669040073	23.493789304090225
36	21.39057221558131	25.48692895379658	29.073229042149578	24.049269788472532
37	20.661088301023856	27.132789356062574	28.184917623824123	24.021204719089447
38	20.86066212774804	27.28278156020997	28.083363650537912	23.77319266150408
39	21.546905046515256	24.980406423782547	28.341666233563746	25.131022296138454
40	21.901252533652098	23.32768567122291	31.070734369315524	23.70032742580947
41	19.988254248739672	24.695286107790658	29.092874590717738	26.223585052751936
42	20.83374045008056	23.52559638272439	29.086949742736863	26.553713424458188
43	21.423418741229668	23.91434956603087	29.169897614469104	25.49233407827036
44	21.3265422795073	24.946000727612912	28.550699028117045	25.176757964762746
45	21.4600072761291	24.054467023543474	28.4589158567642	26.026609843563225
46	21.19099838885713	25.307936177953327	28.754638532300813	24.746426900888725
47	20.629489111792527	23.885141104932174	30.802453094953485	24.682916688321814
48	19.84616184190011	23.939504183774233	30.572007691907903	25.642326282417756
49	20.68790603398992	23.78566602567434	30.236578140429295	25.28984979990645
50	19.237149836287095	24.168702250402784	31.387661763941583	25.206486149368533
51	19.762694246660775	24.053011797723613	28.96377527155553	27.22051868406008
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	306.0
1	783.5
2	1261.0
3	2337.0
4	3413.0
5	2728.5
6	2044.0
7	1788.0
8	1532.0
9	1459.0
10	1386.0
11	1490.5
12	1595.0
13	1564.0
14	1533.0
15	1677.0
16	1821.0
17	1833.5
18	1846.0
19	2056.5
20	2267.0
21	2663.0
22	3059.0
23	3608.5
24	4158.0
25	5432.0
26	7568.0
27	8430.0
28	10505.0
29	12580.0
30	16773.5
31	20967.0
32	21292.5
33	21618.0
34	25358.5
35	29099.0
36	31209.0
37	33319.0
38	37795.0
39	42271.0
40	45606.0
41	48941.0
42	53120.0
43	57299.0
44	62684.5
45	68070.0
46	76699.0
47	85328.0
48	85944.0
49	86560.0
50	83248.0
51	79936.0
52	71380.0
53	62824.0
54	57465.0
55	52106.0
56	48170.0
57	44234.0
58	41478.0
59	38722.0
60	37313.5
61	35905.0
62	33721.0
63	31537.0
64	28103.5
65	24670.0
66	20831.5
67	16993.0
68	14162.0
69	11331.0
70	8925.0
71	6519.0
72	5219.5
73	3920.0
74	3207.0
75	1927.5
76	1361.0
77	1101.0
78	841.0
79	718.5
80	596.0
81	430.5
82	265.0
83	207.0
84	149.0
85	130.0
86	111.0
87	86.5
88	62.0
89	49.0
90	36.0
91	26.0
92	16.0
93	14.0
94	12.0
95	6.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	962050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.79445957513727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.29528678659416	25.850643527193807
2	8.79311775875092	6.1190356079256105
3	3.722563764364696	3.8857378324517464
4	2.3035027557819854	3.205965340690944
5	1.5730503118093986	2.736671774195459
6	1.2263568440539374	2.5602254181076582
7	0.9626461590788017	2.344632700705997
8	0.7530682097056078	2.0962081103938215
9	0.6123506289203123	1.917576828339992
>10	5.5614321113235325	42.02334560068813
>50	0.16441118029323581	3.4743360391713565
>100	0.027351075840718355	1.5789194731843925
>500	0.0030390084266862212	0.8406382140141402
>1k	0.001823405056011733	1.3660635329369406
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	4620	0.4802245205550647	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	2812	0.29229250038979265	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCCTC	1807	0.18782807546385324	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATG	1401	0.1456265266878021	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1275	0.13252949430902758	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1004	0.10436048022452056	RNA PCR Primer, Index 35 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1120523881295151	0.0
2	0.0	0.0	0.0	0.5392651109609687	0.0
3	0.0	0.0	0.0	0.7397744399979211	0.0
4	0.0	0.0	0.0	0.9947507925783483	0.0
5	0.0	0.0	0.0	1.8828543215009614	0.0
6	0.0	0.0	0.0	2.449248999532249	0.0
7	0.0	0.0	0.0	2.9943350137726727	0.0
8	0.0	0.0	0.0	4.030247908112884	0.0
9	0.0	0.0	0.0	4.4318902343953015	0.0
10	0.0	0.0	0.0	5.371342445818825	0.0
11	0.0	0.0	0.0	6.307884205602619	0.0
12	0.0	0.0	0.0	7.155865079777558	0.0
13	0.0	0.0	0.0	7.681929213658334	0.0
14	0.0	0.0	0.0	7.822774284080869	0.0
15	0.0	0.0	0.0	8.22057065641079	0.0
16	0.0	0.0	0.0	8.745803232680213	0.0
17	0.0	0.0	0.0	9.356894132321605	0.0
18	0.0	0.0	0.0	10.02525856244478	0.0
19	0.0	0.0	0.0	10.425549607608753	0.0
20	0.0	0.0	0.0	10.85151499402318	0.0
21	0.0	0.0	0.0	11.334026298009459	0.0
22	0.0	0.0	0.0	11.871004625539213	0.0
23	0.0	0.0	0.0	12.43012317447118	0.0
24	0.0	0.0	0.0	12.881762902136064	0.0
25	0.0	0.0	0.0	13.50761394937893	0.0
26	0.0	0.0	0.0	13.884621381425083	0.0
27	0.0	0.0	0.0	14.275245569357102	0.0
28	0.0	0.0	0.0	14.649550439166363	0.0
29	0.0	0.0	0.0	15.060028065069384	0.0
30	0.0	0.0	0.0	15.50314432721792	0.0
31	1.0394470141884517E-4	0.0	0.0	15.887012109557716	0.0
32	2.0788940283769034E-4	0.0	0.0	16.38251650122135	0.0
33	2.0788940283769034E-4	0.0	0.0	16.776466919598775	0.0
34	2.0788940283769034E-4	0.0	0.0	17.295566758484487	0.0
35	2.0788940283769034E-4	0.0	0.0	17.700535315212306	0.0
36	2.0788940283769034E-4	0.0	0.0	18.087001715087574	0.0
37	2.0788940283769034E-4	0.0	0.0	18.464113091835145	0.0
38	2.0788940283769034E-4	0.0	0.0	18.87854061639208	0.0
39	2.0788940283769034E-4	0.0	0.0	19.346291772776883	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGC	25	3.89033E-5	45.0	44
TGCGACG	40	6.8139343E-9	45.0	1
GCTACGA	80	0.0	45.0	10
TACCGCG	20	7.032714E-4	45.0	1
TTGACCC	1745	0.0	43.32378	8
CTACGAA	85	0.0	42.35294	11
CTCAACT	1745	0.0	41.905445	2
CTTGACC	1710	0.0	41.842106	7
CATTAGA	1620	0.0	41.666664	17
TGCATTA	1650	0.0	41.181816	15
GCTCAAC	1875	0.0	41.04	1
ACTTGAC	1765	0.0	40.66572	6
TCAACTT	1775	0.0	40.563377	3
GCATTAG	1700	0.0	40.102943	16
GTAGACG	45	1.927765E-8	40.0	1
TACGAAT	90	0.0	40.0	12
ACCCTGC	1730	0.0	39.537575	11
CCTGCAT	1730	0.0	39.537575	13
ATTGACC	305	0.0	39.098362	7
TGACCCT	1755	0.0	38.974358	9
>>END_MODULE