##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936072.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	642246
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84692158456417	31.0	31.0	34.0	30.0	34.0
2	31.9755047131473	33.0	31.0	34.0	30.0	34.0
3	31.909170317915564	33.0	31.0	34.0	30.0	34.0
4	35.651337649436506	37.0	35.0	37.0	33.0	37.0
5	31.120804177838398	37.0	35.0	37.0	0.0	37.0
6	33.332204482394594	37.0	35.0	37.0	17.0	37.0
7	29.506771548596642	35.0	32.0	37.0	0.0	37.0
8	32.48502598692713	35.0	33.0	37.0	17.0	37.0
9	36.35729299987855	39.0	35.0	39.0	32.0	39.0
10	36.7471887719036	38.0	35.0	39.0	33.0	39.0
11	36.85346269186574	39.0	37.0	39.0	33.0	39.0
12	36.81693618955976	39.0	35.0	39.0	33.0	39.0
13	36.562325028104496	39.0	35.0	39.0	32.0	39.0
14	37.670534343538144	40.0	37.0	41.0	33.0	41.0
15	37.75248425058311	40.0	36.0	41.0	33.0	41.0
16	37.88684709597257	40.0	36.0	41.0	33.0	41.0
17	37.83441703023452	40.0	36.0	41.0	33.0	41.0
18	37.74601788099887	40.0	36.0	41.0	33.0	41.0
19	37.64914067195436	40.0	36.0	41.0	33.0	41.0
20	37.60170401995497	39.0	36.0	41.0	32.0	41.0
21	37.500104321397096	39.0	36.0	41.0	32.0	41.0
22	37.55188043210857	39.0	36.0	41.0	33.0	41.0
23	37.53184138165127	39.0	36.0	41.0	33.0	41.0
24	37.43834761135141	39.0	35.0	41.0	32.0	41.0
25	37.29704817157289	39.0	35.0	41.0	32.0	41.0
26	37.11942775821103	39.0	35.0	41.0	32.0	41.0
27	37.06310977413639	39.0	35.0	41.0	32.0	41.0
28	36.92975900200235	39.0	35.0	41.0	31.0	41.0
29	36.88323477296861	39.0	35.0	41.0	31.0	41.0
30	36.74372436729851	39.0	35.0	41.0	31.0	41.0
31	36.539550265786005	39.0	35.0	40.0	30.0	41.0
32	36.46045751939288	39.0	35.0	40.0	30.0	41.0
33	36.272356698212214	39.0	35.0	40.0	30.0	41.0
34	36.11504470249717	39.0	35.0	40.0	29.0	41.0
35	36.02558365486122	39.0	35.0	40.0	29.0	41.0
36	35.76435509135129	38.0	35.0	40.0	27.0	41.0
37	35.70802309395465	38.0	35.0	40.0	27.0	41.0
38	35.38459873630976	38.0	34.0	40.0	26.0	41.0
39	35.366102708308034	38.0	34.0	40.0	26.0	41.0
40	35.17316417696646	38.0	34.0	40.0	25.0	41.0
41	34.99745424650368	38.0	34.0	40.0	24.0	41.0
42	34.98329456314247	38.0	34.0	40.0	24.0	41.0
43	34.79152847974141	38.0	34.0	40.0	24.0	41.0
44	34.623416883873155	38.0	33.0	40.0	23.0	41.0
45	34.4853887762633	37.0	33.0	40.0	23.0	41.0
46	34.30614904569277	37.0	33.0	40.0	23.0	41.0
47	34.115734469346634	37.0	33.0	40.0	22.0	41.0
48	33.98886252308306	37.0	33.0	40.0	22.0	41.0
49	33.856900626862604	37.0	33.0	40.0	21.0	41.0
50	33.699583960040236	36.0	33.0	40.0	20.0	41.0
51	31.59411658461088	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	21.0
10	23.0
11	29.0
12	22.0
13	37.0
14	29.0
15	67.0
16	106.0
17	223.0
18	442.0
19	792.0
20	1175.0
21	1872.0
22	2743.0
23	4054.0
24	5388.0
25	7215.0
26	8857.0
27	10566.0
28	11746.0
29	13452.0
30	16048.0
31	20068.0
32	25437.0
33	34944.0
34	47850.0
35	55330.0
36	65070.0
37	95966.0
38	112333.0
39	100313.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.561046701731115	20.211102910722683	23.408164472803257	10.81968591474295
2	31.715106049706808	26.07225268822227	25.03308701027332	17.179554251797597
3	32.49954067444561	25.812539120523915	25.753838871709593	15.934081333320876
4	28.03224932502499	25.297938796037656	27.187246008538786	19.48256587039857
5	22.972505862239704	38.59300018995837	21.350697396324772	17.08379655147716
6	25.207163610205434	36.0762075590973	24.3431644572329	14.373464373464373
7	68.90365996829874	19.330443474930167	8.02169262245308	3.744203934318003
8	82.62597197958415	6.348501975878401	7.460069817484266	3.5654562270531853
9	76.9189687440638	6.990623530547485	8.775765049529308	7.314642675859407
10	43.195286541294145	25.679256857964084	16.52637774310778	14.599078857633991
11	31.55986958268327	25.124173603261056	23.985669042703265	19.33028777135241
12	28.75175555783921	22.69877897254323	28.291028671256807	20.25843679836075
13	24.656751462835114	24.938730642152695	31.037328375731416	19.367189519280775
14	21.736219454850634	26.40358990168876	30.189210987690075	21.67097965577053
15	19.835390177595503	24.919111991355336	34.71520258592502	20.53029524512414
16	24.943090342329885	25.333127804610694	28.303484957477355	21.420296895582066
17	25.01362406305372	24.507120324610817	27.600016193172088	22.879239419163376
18	25.742005399800078	24.31171233452602	28.892355888553606	21.053926377120295
19	24.974231057881248	27.06922269659912	26.086577417375896	21.869968828143733
20	25.78715943734955	26.740221036798985	27.898655655309646	19.573963870541817
21	24.71436178660513	27.868760568380342	27.832793041918517	19.58408460309601
22	24.4952868527013	22.95319861859786	28.577834661484854	23.97367986721599
23	22.75249670686933	26.790357588836677	28.639804685432065	21.81734101886193
24	23.287494200041728	24.57002457002457	29.30247911236504	22.84000211756866
25	23.447868885131243	27.41005782830878	26.806550760923386	22.335522525636595
26	23.47932100783812	25.607633212195953	26.18015526760774	24.732890512358193
27	22.75638929631325	26.222039530024322	27.430299293417164	23.591271880245266
28	20.184789006081783	26.908692307931855	28.413567386951417	24.49295129903495
29	22.46989471324072	25.945042865194957	27.048669824335224	24.5363925972291
30	23.294033750307513	25.310395082258204	28.31142583994295	23.084145327491335
31	24.936862199219615	26.249287656131763	26.219548272780212	22.59430187186841
32	25.294980428060278	25.349320976697403	26.176106974586062	23.17959162065626
33	24.409027070624028	24.945581599573995	26.138426708768915	24.506964621033063
34	21.814226947306796	25.24063987942315	29.371300093733556	23.573833079536502
35	21.54906375438695	26.356100310472936	28.355178545292613	23.739657389847505
36	24.680418406654148	27.281446673081653	26.157422545255244	21.880712375008954
37	22.171255251103158	26.70331928887062	26.887516621356927	24.237908838669295
38	22.574060406760026	27.429832182683896	27.141313453100523	22.854793957455556
39	23.57928270475799	24.159278531902107	27.750270145707407	24.511168617632496
40	24.87847335756081	23.281733167664726	29.76787710628015	22.071916368494314
41	22.134042096019282	24.57921108111222	28.185773052693204	25.10097377017529
42	22.540584137542314	23.827474207702345	29.699211828489396	23.93272982626595
43	23.381383457429084	24.34954830392093	27.32255241760945	24.946515821040535
44	22.69877897254323	24.13374314514999	28.00702534542839	25.160452536878392
45	21.7320154582512	23.964181948972822	27.204217698514277	27.099584894261703
46	23.463906353640194	26.02445168985093	26.569414211999764	23.94222774450911
47	20.91021197485076	24.838613241655068	30.69353487604438	23.557639907449794
48	21.99359746888264	26.294285990103482	28.123491621590478	23.5886249194234
49	22.809017105595053	24.329151135234785	28.359071134736535	24.502760624433627
50	20.958012973222097	25.89770897755689	28.817773874808093	24.326504174412918
51	21.92088389807021	24.57780974891241	27.14100204594501	26.36030430707237
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	199.0
1	593.0
2	987.0
3	2058.0
4	3129.0
5	2404.0
6	1679.0
7	1440.0
8	1201.0
9	1189.5
10	1178.0
11	1142.5
12	1107.0
13	1129.5
14	1152.0
15	1120.0
16	1088.0
17	1078.0
18	1068.0
19	1280.5
20	1493.0
21	1580.0
22	1667.0
23	2210.0
24	2753.0
25	3483.0
26	4925.5
27	5638.0
28	6633.0
29	7628.0
30	8968.0
31	10308.0
32	12076.0
33	13844.0
34	15811.0
35	17778.0
36	19066.5
37	20355.0
38	23080.5
39	25806.0
40	27872.0
41	29938.0
42	32198.5
43	34459.0
44	38770.5
45	43082.0
46	49569.5
47	56057.0
48	53213.0
49	50369.0
50	49693.5
51	49018.0
52	46490.5
53	43963.0
54	41272.0
55	38581.0
56	36873.0
57	35165.0
58	33425.5
59	31686.0
60	29910.5
61	28135.0
62	25544.0
63	22953.0
64	19976.5
65	17000.0
66	14609.5
67	12219.0
68	11084.0
69	9949.0
70	7992.0
71	6035.0
72	4812.5
73	3590.0
74	3081.0
75	2024.0
76	1476.0
77	1144.5
78	813.0
79	575.5
80	338.0
81	275.5
82	213.0
83	182.0
84	151.0
85	116.0
86	81.0
87	68.5
88	56.0
89	50.0
90	44.0
91	34.5
92	25.0
93	15.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	642246.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.07221188226739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38490522824928	27.193002741180543
2	7.757372906855385	5.596511982916959
3	3.535748390435457	3.8262679530652113
4	2.174257876891709	3.1372116328770625
5	1.6495516975706594	2.975148917276134
6	1.2025187011584102	2.6026505628345094
7	0.942837364511518	2.3807160428224634
8	0.730076918165191	2.1068391425926047
9	0.6048245030324363	1.9635621862475805
>10	5.923325989763005	43.2427095848782
>50	0.06762058306695107	1.53958079521464
>100	0.023424123539374706	1.6455993542966503
>500	0.0017678583803301664	0.4084504500782812
>1k	0.0017678583803301664	1.3817486537191501
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	2856	0.4446894180734485	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	2537	0.39501997676902617	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	2089	0.32526477393397546	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1185	0.1845087396418195	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	859	0.13374937329309952	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.557035777568097E-4	0.0	0.0	0.05153788423750401	0.0
2	1.557035777568097E-4	0.0	0.0	0.33024728842219336	0.0
3	1.557035777568097E-4	0.0	0.0	0.42304662076525196	0.0
4	1.557035777568097E-4	0.0	0.0	0.6340249686257291	0.0
5	1.557035777568097E-4	0.0	0.0	1.5539217060129609	0.0
6	1.557035777568097E-4	0.0	0.0	1.910482899076055	0.0
7	1.557035777568097E-4	0.0	0.0	2.3369549985519567	0.0
8	1.557035777568097E-4	0.0	0.0	2.936413772915674	0.0
9	1.557035777568097E-4	0.0	0.0	3.1690349180843476	0.0
10	1.557035777568097E-4	0.0	0.0	4.4490740308230805	0.0
11	1.557035777568097E-4	0.0	0.0	5.044951622898391	0.0
12	1.557035777568097E-4	0.0	0.0	6.165705975591907	0.0
13	1.557035777568097E-4	0.0	0.0	6.391164756183768	0.0
14	1.557035777568097E-4	0.0	0.0	6.498444521258209	0.0
15	1.557035777568097E-4	0.0	0.0	6.72779589129399	0.0
16	1.557035777568097E-4	0.0	0.0	7.0851356022458685	0.0
17	1.557035777568097E-4	0.0	0.0	7.481089800481436	0.0
18	1.557035777568097E-4	0.0	0.0	7.9181497432448005	0.0
19	1.557035777568097E-4	0.0	0.0	8.185959896986512	0.0
20	1.557035777568097E-4	0.0	0.0	8.445829168262629	0.0
21	1.557035777568097E-4	0.0	0.0	8.776699270995849	0.0
22	1.557035777568097E-4	0.0	0.0	9.152723411278544	0.0
23	1.557035777568097E-4	0.0	0.0	9.506014829208745	0.0
24	1.557035777568097E-4	0.0	0.0	9.782388679727083	0.0
25	1.557035777568097E-4	0.0	0.0	10.054091422912716	0.0
26	1.557035777568097E-4	0.0	0.0	10.298234632835394	0.0
27	1.557035777568097E-4	0.0	0.0	10.583483587285869	0.0
28	1.557035777568097E-4	0.0	0.0	10.846155522961606	0.0
29	1.557035777568097E-4	0.0	0.0	11.15055601747617	0.0
30	1.557035777568097E-4	0.0	0.0	11.549935694422386	0.0
31	1.557035777568097E-4	0.0	0.0	11.832226280895483	0.0
32	1.557035777568097E-4	0.0	0.0	12.121056417634364	0.0
33	1.557035777568097E-4	0.0	0.0	12.416893215372303	0.0
34	1.557035777568097E-4	0.0	0.0	12.736085549773764	0.0
35	1.557035777568097E-4	0.0	0.0	13.087352821193125	0.0
36	1.557035777568097E-4	0.0	0.0	13.388639244152552	0.0
37	1.557035777568097E-4	0.0	0.0	13.70347187837682	0.0
38	1.557035777568097E-4	0.0	0.0	14.071866543349433	0.0
39	1.557035777568097E-4	0.0	0.0	14.674750796423801	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATACT	25	3.8888757E-5	45.000004	25
AGCGTAA	20	7.03096E-4	45.0	33
CAACCGC	20	7.03096E-4	45.0	16
GCTAACG	35	1.2108103E-7	45.0	1
TTACGGG	165	0.0	42.272728	3
TACGGCT	485	0.0	41.75258	7
CGTTATT	125	0.0	41.4	1
CGGGACC	185	0.0	40.135136	6
TCTACGG	45	1.925946E-8	40.0	2
AGACACG	165	0.0	39.545456	24
CGCATGG	75	0.0	39.0	2
TAATACG	110	0.0	38.863636	4
CCCTCGA	35	6.243985E-6	38.571426	23
TACGAAT	175	0.0	38.571426	12
CGTACAT	35	6.243985E-6	38.571426	35
TAGACGG	95	0.0	37.894737	2
AACGGGA	185	0.0	37.7027	4
CCGATAC	30	1.13936076E-4	37.500004	18
AACGATT	30	1.13936076E-4	37.500004	10
AACGAGT	30	1.13936076E-4	37.500004	29
>>END_MODULE