FastQCFastQC Report
Sat 14 Jan 2017
SRR2936071.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936071.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752325
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47680.6337686505167315No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14190.18861529259296184No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.12335094540258532No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8940.11883162197188715No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGA207.031732E-445.09
CGACGGT253.8895167E-545.028
TCTCGTA351.2111741E-745.028
CGCTAAA207.031732E-445.044
CGGTCTA207.031732E-445.031
CGCCAAT207.031732E-445.030
CGTTATT4050.043.8888851
CTCGATC650.041.53846416
CGTTTTT21000.039.5357171
GCTACGA1500.039.010
TAGCACG700.038.5714261
TCGTACG356.245413E-638.57142630
CCTCGAT601.546141E-1037.50000415
CGGTTTA850.037.0588231
CCCTCGT552.746674E-936.81818414
CGTTGAT552.746674E-936.81818425
CCTCGTG552.746674E-936.81818415
TACGAAT1600.036.562512
CGAATAT1600.036.562514
ACGGGCT1850.036.4864855
Produced by FastQC (version 0.11.5)