##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936071.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 752325 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.964068720300403 33.0 31.0 34.0 30.0 34.0 2 32.090744026850096 33.0 31.0 34.0 30.0 34.0 3 31.824577144186357 33.0 31.0 34.0 30.0 34.0 4 35.653197753630415 37.0 35.0 37.0 33.0 37.0 5 31.21867012261988 37.0 35.0 37.0 0.0 37.0 6 33.45808659821221 37.0 35.0 37.0 17.0 37.0 7 29.52085335460074 35.0 32.0 37.0 0.0 37.0 8 32.61317116937494 35.0 33.0 37.0 17.0 37.0 9 36.52521184328582 39.0 35.0 39.0 32.0 39.0 10 36.907278104542584 38.0 37.0 39.0 34.0 39.0 11 37.0704097298375 39.0 37.0 39.0 34.0 39.0 12 37.139843817499084 39.0 37.0 39.0 34.0 39.0 13 37.05008473731433 39.0 37.0 39.0 33.0 39.0 14 38.36349981723324 40.0 38.0 41.0 34.0 41.0 15 38.43390024258133 40.0 38.0 41.0 34.0 41.0 16 38.475588342803974 40.0 38.0 41.0 34.0 41.0 17 38.43764463496494 40.0 38.0 41.0 34.0 41.0 18 38.320543648024454 40.0 38.0 41.0 34.0 41.0 19 38.197349549729175 40.0 37.0 41.0 34.0 41.0 20 38.090817133552655 40.0 37.0 41.0 34.0 41.0 21 37.98806234007909 40.0 37.0 41.0 33.0 41.0 22 37.946726481241484 40.0 36.0 41.0 33.0 41.0 23 37.89154952979098 40.0 36.0 41.0 33.0 41.0 24 37.84630711461137 40.0 36.0 41.0 33.0 41.0 25 37.70732861462799 40.0 36.0 41.0 33.0 41.0 26 37.554228558136444 40.0 36.0 41.0 33.0 41.0 27 37.51636327385106 40.0 36.0 41.0 33.0 41.0 28 37.40284318612302 40.0 36.0 41.0 33.0 41.0 29 37.372806965074936 40.0 36.0 41.0 33.0 41.0 30 37.2582540790217 39.0 35.0 41.0 32.0 41.0 31 37.14572425481009 39.0 35.0 41.0 32.0 41.0 32 37.03808460439305 39.0 35.0 41.0 31.0 41.0 33 36.827841690758646 39.0 35.0 41.0 31.0 41.0 34 36.598294620011295 39.0 35.0 41.0 30.0 41.0 35 36.4386083142259 39.0 35.0 41.0 30.0 41.0 36 36.30249293855714 39.0 35.0 41.0 30.0 41.0 37 36.13808526899943 39.0 35.0 41.0 29.0 41.0 38 36.01345960854684 39.0 35.0 41.0 28.0 41.0 39 35.99765261024159 39.0 35.0 41.0 28.0 41.0 40 35.86656830492141 39.0 35.0 40.0 27.0 41.0 41 35.75753298109195 39.0 35.0 40.0 27.0 41.0 42 35.69659522148008 39.0 35.0 40.0 27.0 41.0 43 35.553431030472204 39.0 35.0 40.0 26.0 41.0 44 35.40489017379457 38.0 35.0 40.0 25.0 41.0 45 35.31371548200578 38.0 34.0 40.0 25.0 41.0 46 35.201634931711695 38.0 34.0 40.0 24.0 41.0 47 35.09718273352607 38.0 34.0 40.0 24.0 41.0 48 35.00340943076463 38.0 34.0 40.0 24.0 41.0 49 34.81906622802645 38.0 34.0 40.0 24.0 41.0 50 34.67363572923936 38.0 34.0 40.0 23.0 41.0 51 32.83704781842953 36.0 31.0 39.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 12.0 9 24.0 10 37.0 11 28.0 12 28.0 13 26.0 14 42.0 15 67.0 16 91.0 17 168.0 18 316.0 19 612.0 20 982.0 21 1633.0 22 2494.0 23 3692.0 24 5561.0 25 7815.0 26 9777.0 27 10811.0 28 11678.0 29 12440.0 30 14825.0 31 18428.0 32 23430.0 33 33063.0 34 47741.0 35 59467.0 36 71360.0 37 116398.0 38 149360.0 39 149897.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.89432758448809 20.93696208420563 25.241218888113515 13.92749144319277 2 34.45385970159173 24.472136377230587 24.518392981756556 16.55561093942113 3 31.72352374306317 23.63433356594557 28.465889077194035 16.17625361379723 4 28.23792908649852 25.557970292094506 27.979928887116607 18.22417173429037 5 21.378792410195064 38.767022231083644 23.14225899710896 16.711926361612335 6 25.31818030771276 35.066228026451334 26.19453028943608 13.421061376399827 7 67.7023892599608 19.747715415545144 8.851094939022364 3.6988003854717046 8 82.08433855049347 5.522347389758416 9.36496859734822 3.0283454623998938 9 75.87133220350248 8.24324593759346 10.940484498056026 4.944937360848037 10 38.67384441564484 28.87342571362111 18.81327883560961 13.639451035124447 11 28.45764795799688 27.109826205429833 26.994849300501777 17.43767653607151 12 26.441498022796 24.6429402186555 30.40361545874456 18.51194629980394 13 23.822151330874288 24.403947761937992 31.68524241517961 20.088658492008108 14 20.141029475293255 27.386435383644038 31.100787558568438 21.37174758249427 15 20.1132489283222 27.69295184926727 33.34356827169109 18.85023095071944 16 22.72608247765261 26.43192769082511 32.12002791346825 18.721961918054035 17 22.78842255674077 26.196125344764564 29.915794370783903 21.099657727710763 18 22.62320140896554 26.71717675206859 30.48629249327086 20.173329345695013 19 23.972352374306315 27.812580998903396 28.592828897085703 19.622237729704583 20 25.30887581829661 26.42235735885422 29.749908616621806 18.518858206227364 21 24.31396005715615 26.83773635064633 29.795234772206157 19.05306881999136 22 23.287940717110292 25.505200544977235 29.62921609676669 21.57764264114578 23 22.55049347024225 26.956567972618217 30.461967899511517 20.030970657628018 24 21.813046223374208 25.809856112717245 31.09852789685309 21.278569767055462 25 21.944904130528695 28.000930448941613 29.163061177017912 20.89110424351178 26 21.377064433589208 28.475991094274416 29.07559897650616 21.071345495630215 27 21.314857275778422 28.373242946864718 30.326654039145318 19.985245738211542 28 20.78862193865683 28.128933639052274 30.685807330608444 20.39663709168245 29 21.471571461801748 27.076728807363835 30.836672980427345 20.615026750407072 30 21.1160070448277 27.045891071013195 31.01319243677932 20.824909447379788 31 22.505300235935266 26.909779682982755 29.443790914830693 21.14112916625129 32 22.80038547170438 27.868673777955006 29.614727677532983 19.71621307280763 33 22.189479280895892 27.454225235104506 29.48725617253182 20.869039311467784 34 21.67666899278902 28.115242747482803 29.679726182168608 20.528362077559564 35 22.503306416774667 26.497989565679724 29.912870102681687 21.085833914863922 36 22.537334263782274 28.217725052337755 28.566377562888377 20.67856312099159 37 22.29236034958296 28.95105173960722 29.42651114877214 19.330076762037685 38 22.437244541920048 27.940185425181934 28.40727079387233 21.215299239025686 39 22.30339281560496 27.523078456783967 28.64639617186721 21.52713255574386 40 23.245538829628153 27.005615923969028 29.555976472933903 20.192868773468913 41 20.742099491576113 27.92995048682418 29.945967500747685 21.381982520852024 42 22.072375635529855 27.969029342371982 29.105107500083076 20.853487522015087 43 22.562988070315356 27.18426212075898 29.25358056690925 20.999169242016418 44 22.134715714618018 27.61266739773369 28.84458179643106 21.408035091217226 45 22.26325058983817 27.26188814674509 27.90974645266341 22.565114810753332 46 21.533778619612534 28.867975941248798 27.887681520619413 21.710563918519256 47 22.180174791479747 27.157810786561658 29.8857541620975 20.776260259861097 48 22.04831688432526 26.421559831189978 29.874057089688634 21.656066194796132 49 21.057521682783374 27.27611072342405 29.84973249592929 21.81663509786329 50 20.030040208686405 27.283288472402216 30.346725152028707 22.33994616688266 51 21.163592862127405 26.915229455355068 28.55135745854518 23.36982022397235 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 396.0 1 893.5 2 1391.0 3 6144.0 4 10897.0 5 8376.5 6 5856.0 7 4655.0 8 3454.0 9 3314.5 10 3175.0 11 3142.5 12 3110.0 13 2996.0 14 2882.0 15 2871.5 16 2861.0 17 2688.5 18 2516.0 19 2796.0 20 3076.0 21 3293.5 22 3511.0 23 4020.0 24 4529.0 25 5262.5 26 7554.0 27 9112.0 28 11165.0 29 13218.0 30 15507.5 31 17797.0 32 19938.5 33 22080.0 34 24931.0 35 27782.0 36 29200.0 37 30618.0 38 32402.5 39 34187.0 40 37537.0 41 40887.0 42 43192.5 43 45498.0 44 47918.5 45 50339.0 46 53856.0 47 57373.0 48 57515.0 49 57657.0 50 54948.5 51 52240.0 52 49036.0 53 45832.0 54 42092.0 55 38352.0 56 35707.0 57 33062.0 58 31470.0 59 29878.0 60 28947.5 61 28017.0 62 24952.0 63 21887.0 64 18514.0 65 15141.0 66 12532.0 67 9923.0 68 8175.0 69 6427.0 70 5347.0 71 4267.0 72 3443.5 73 2620.0 74 2268.5 75 1481.0 76 1045.0 77 847.0 78 649.0 79 504.5 80 360.0 81 295.5 82 231.0 83 181.0 84 131.0 85 102.5 86 74.0 87 60.5 88 47.0 89 32.0 90 17.0 91 12.5 92 8.0 93 4.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 752325.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.830272301191876 #Duplication Level Percentage of deduplicated Percentage of total 1 72.9990571991874 26.155760971771656 2 8.15377609482491 5.843040355210508 3 4.061305473576027 4.365530430496502 4 2.6269994399585523 3.7650442107517397 5 1.9171014703741471 3.4345133856260515 6 1.3698039404804474 2.944826891199604 7 1.0347627851863586 2.595308265225686 8 0.882154713850565 2.52862748872366 9 0.6899345937365431 2.2248489927233277 >10 6.177294785691355 41.78914929920469 >50 0.059817828306495104 1.3758267082033682 >100 0.02569098922495654 1.692236563382803 >500 0.0015337904014899372 0.4352525316876438 >1k 7.668952007449686E-4 0.8500339057927563 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4768 0.6337686505167315 No Hit CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1419 0.18861529259296184 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 928 0.12335094540258532 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 894 0.11883162197188715 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.032565712956501514 0.0 2 0.0 0.0 0.0 0.16561991160735054 0.0 3 0.0 0.0 0.0 0.27142524839663706 0.0 4 0.0 0.0 0.0 0.4040806832153657 0.0 5 0.0 0.0 0.0 0.8218522580001993 0.0 6 0.0 0.0 0.0 1.2055959857774232 0.0 7 0.0 0.0 0.0 1.4876549363639384 0.0 8 0.0 0.0 0.0 1.936397168776792 0.0 9 0.0 0.0 0.0 2.1461469444721364 0.0 10 0.0 0.0 0.0 2.66640082411192 0.0 11 1.3292127737347557E-4 0.0 0.0 3.23969029342372 0.0 12 1.3292127737347557E-4 0.0 0.0 3.718871498355099 0.0 13 1.3292127737347557E-4 0.0 0.0 3.932608912371648 0.0 14 1.3292127737347557E-4 0.0 0.0 4.03176818529226 0.0 15 1.3292127737347557E-4 0.0 0.0 4.186488552154986 0.0 16 1.3292127737347557E-4 0.0 0.0 4.487289402851161 0.0 17 1.3292127737347557E-4 0.0 0.0 4.833416409131692 0.0 18 1.3292127737347557E-4 0.0 0.0 5.206393513441665 0.0 19 1.3292127737347557E-4 0.0 0.0 5.442727544611704 0.0 20 1.3292127737347557E-4 0.0 0.0 5.6701558501977205 0.0 21 1.3292127737347557E-4 0.0 0.0 5.956734124214933 0.0 22 1.3292127737347557E-4 0.0 0.0 6.26484564516665 0.0 23 1.3292127737347557E-4 0.0 0.0 6.572824244840993 0.0 24 1.3292127737347557E-4 0.0 0.0 6.811683780281128 0.0 25 1.3292127737347557E-4 0.0 0.0 7.047353205064301 0.0 26 1.3292127737347557E-4 0.0 0.0 7.260160170139235 0.0 27 1.3292127737347557E-4 0.0 0.0 7.46990994583458 0.0 28 1.3292127737347557E-4 0.0 0.0 7.6905592662745486 0.0 29 1.3292127737347557E-4 0.0 0.0 7.926494533612468 0.0 30 1.3292127737347557E-4 0.0 0.0 8.193400458578408 0.0 31 1.3292127737347557E-4 0.0 0.0 8.445817964310637 0.0 32 2.6584255474695114E-4 0.0 0.0 8.688532216794604 0.0 33 2.6584255474695114E-4 0.0 0.0 8.917422656431729 0.0 34 3.9876383212042666E-4 0.0 0.0 9.175289934536272 0.0 35 3.9876383212042666E-4 0.0 0.0 9.437676536071512 0.0 36 3.9876383212042666E-4 0.0 0.0 9.674143488518924 0.0 37 3.9876383212042666E-4 0.0 0.0 9.946100422025056 0.0 38 3.9876383212042666E-4 0.0 0.0 10.215664772538464 0.0 39 3.9876383212042666E-4 0.0 0.0 10.498388329511847 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGACGA 20 7.031732E-4 45.0 9 CGACGGT 25 3.8895167E-5 45.0 28 TCTCGTA 35 1.2111741E-7 45.0 28 CGCTAAA 20 7.031732E-4 45.0 44 CGGTCTA 20 7.031732E-4 45.0 31 CGCCAAT 20 7.031732E-4 45.0 30 CGTTATT 405 0.0 43.888885 1 CTCGATC 65 0.0 41.538464 16 CGTTTTT 2100 0.0 39.535717 1 GCTACGA 150 0.0 39.0 10 TAGCACG 70 0.0 38.571426 1 TCGTACG 35 6.245413E-6 38.571426 30 CCTCGAT 60 1.546141E-10 37.500004 15 CGGTTTA 85 0.0 37.058823 1 CCCTCGT 55 2.746674E-9 36.818184 14 CGTTGAT 55 2.746674E-9 36.818184 25 CCTCGTG 55 2.746674E-9 36.818184 15 TACGAAT 160 0.0 36.5625 12 CGAATAT 160 0.0 36.5625 14 ACGGGCT 185 0.0 36.486485 5 >>END_MODULE