##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936066.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	671971
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.839360627169924	31.0	31.0	34.0	30.0	34.0
2	31.93851966825949	33.0	31.0	34.0	30.0	34.0
3	31.461927672473962	33.0	31.0	34.0	28.0	34.0
4	35.41506106662341	37.0	35.0	37.0	33.0	37.0
5	30.97002251585262	37.0	35.0	37.0	0.0	37.0
6	33.31247032982078	37.0	35.0	37.0	17.0	37.0
7	29.733198307665063	35.0	32.0	37.0	0.0	37.0
8	32.77657964406202	35.0	35.0	37.0	17.0	37.0
9	36.632595751900006	39.0	35.0	39.0	32.0	39.0
10	36.94231745119953	39.0	37.0	39.0	34.0	39.0
11	37.06553705442646	39.0	37.0	39.0	34.0	39.0
12	37.16126439980297	39.0	37.0	39.0	34.0	39.0
13	37.061559204191845	39.0	37.0	39.0	33.0	39.0
14	38.40120034941984	40.0	38.0	41.0	34.0	41.0
15	38.47219448458341	40.0	38.0	41.0	34.0	41.0
16	38.49086642131878	40.0	38.0	41.0	34.0	41.0
17	38.45936506188511	40.0	38.0	41.0	34.0	41.0
18	38.31972659534414	40.0	38.0	41.0	34.0	41.0
19	38.16032983566255	40.0	37.0	41.0	34.0	41.0
20	37.97802434926507	40.0	36.0	41.0	34.0	41.0
21	37.880713602223906	40.0	36.0	41.0	33.0	41.0
22	37.837399233002614	40.0	36.0	41.0	33.0	41.0
23	37.76235432779093	39.0	36.0	41.0	33.0	41.0
24	37.70842342898726	40.0	35.0	41.0	33.0	41.0
25	37.582691812593104	39.0	35.0	41.0	33.0	41.0
26	37.44197443044417	39.0	35.0	41.0	33.0	41.0
27	37.39804247504728	39.0	35.0	41.0	33.0	41.0
28	37.28379052072188	39.0	35.0	41.0	33.0	41.0
29	37.27822778066315	39.0	35.0	41.0	33.0	41.0
30	37.149372517564004	39.0	35.0	41.0	32.0	41.0
31	37.00335282326172	39.0	35.0	41.0	32.0	41.0
32	36.815707820724406	39.0	35.0	41.0	31.0	41.0
33	36.542222804257925	39.0	35.0	41.0	30.0	41.0
34	36.25132781027753	39.0	35.0	41.0	30.0	41.0
35	35.99101895766335	39.0	35.0	41.0	28.0	41.0
36	35.79104008952768	39.0	35.0	41.0	26.0	41.0
37	35.60776729948167	39.0	35.0	41.0	25.0	41.0
38	35.46810502238936	39.0	35.0	40.0	25.0	41.0
39	35.433874378507404	39.0	35.0	40.0	24.0	41.0
40	35.30747160219712	39.0	35.0	40.0	24.0	41.0
41	35.20732293506714	39.0	35.0	40.0	23.0	41.0
42	35.14943650842075	39.0	35.0	40.0	23.0	41.0
43	35.01024746603648	38.0	34.0	40.0	23.0	41.0
44	34.86258633185063	38.0	34.0	40.0	22.0	41.0
45	34.76834863409284	38.0	34.0	40.0	22.0	41.0
46	34.69472640932421	38.0	34.0	40.0	22.0	41.0
47	34.59670432206152	38.0	34.0	40.0	22.0	41.0
48	34.50675847618424	38.0	34.0	40.0	21.0	41.0
49	34.3644085831085	38.0	34.0	40.0	20.0	41.0
50	34.21038705539376	38.0	33.0	40.0	20.0	41.0
51	32.47815605137722	36.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	29.0
10	41.0
11	27.0
12	23.0
13	18.0
14	20.0
15	35.0
16	82.0
17	139.0
18	246.0
19	531.0
20	915.0
21	1621.0
22	2455.0
23	3818.0
24	6166.0
25	9189.0
26	11602.0
27	12528.0
28	12702.0
29	12532.0
30	13734.0
31	16773.0
32	21031.0
33	29088.0
34	41352.0
35	52255.0
36	61985.0
37	100257.0
38	130241.0
39	130509.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.85137900296293	20.14967907841261	24.46251400730091	16.536427911323553
2	36.15736393386024	23.59967915282058	24.77577157347564	15.467185339843533
3	31.935753179824722	22.753809316175847	30.26112734031677	15.049310163682659
4	26.46721361487326	25.2094212399047	30.97618796049234	17.3471771847297
5	20.632735638889176	38.31102830330476	25.837722163605275	15.218513894200791
6	23.7727520979328	33.921106714426664	29.211528473699012	13.094612713941526
7	66.93131102383883	18.803638847509788	11.000921170705283	3.264128957946102
8	81.35365960733425	4.354205761855794	11.687111497371166	2.6050231334387943
9	75.64835387241415	7.2599859220115155	13.298341743914543	3.793318461659804
10	36.27656550654716	31.932032781176567	20.514278145931893	11.27712356634438
11	26.377477599479736	26.069130959520574	31.45656583394224	16.096825607057447
12	25.122661543429704	23.5266105233708	33.50248745853616	17.848240474663342
13	22.233995217055497	24.320245962995426	34.85596848673529	18.589790333213784
14	18.949032026679724	27.399247884209288	34.03718315224913	19.61453693686186
15	18.754529585354128	27.323351751786905	36.87301981781952	17.049098845039442
16	21.90094513007258	25.47193256851858	35.44840476746764	17.178717533941196
17	21.935916877365244	25.947399515752913	32.43637002192059	19.680313584961258
18	21.68129279388545	26.22642941436461	33.76574286687967	18.32653492487027
19	22.68803266807645	27.362341529619584	31.933669756581757	18.015956045722213
20	24.110713111131286	26.71290874159748	32.33725860193372	16.83911954533752
21	23.552802129853816	26.444444775146547	32.46404978786287	17.538703307136768
22	22.110329165990795	25.305556340973045	32.51821879218002	20.06589570085614
23	21.320711756906178	26.51081668702965	33.35515967206918	18.81331188399499
24	20.907449875069016	25.81301871658152	33.85756230551616	19.421969102833305
25	20.641069331861047	27.498359304196164	32.303328566262536	19.557242797680257
26	20.39820170810943	28.653617492421546	31.98932692035817	18.958853879110855
27	20.656248558345524	28.172049091404244	32.582953728657934	18.588748621592302
28	19.122402603683792	28.68308304971494	33.366618499905506	18.827895846695768
29	20.319180440822596	27.003099836153645	33.052021590217436	19.625698132806328
30	20.968613228844696	26.84580138130961	32.79843921835913	19.387146171486567
31	21.000608657218837	27.16858316802362	31.919532241718763	19.911275933038777
32	21.456283083644976	27.990791269266087	31.97593348522481	18.57699216186413
33	21.67266146902173	28.440661873801105	30.929310937525578	18.95736571965159
34	20.529903820254148	27.989600741698673	31.69541542715385	19.785080010893328
35	20.80952898264955	27.57767820337485	31.805688043085194	19.80710477089041
36	20.76964630914132	29.594729534459074	30.12510956574019	19.51051459065942
37	21.359552718792923	29.58461005013609	30.169903165463985	18.885934065607
38	21.417293305812304	28.715376109980937	29.694882666067436	20.172447918139326
39	22.083839927615923	28.078741493308492	29.757831811194237	20.079586767881352
40	22.17298067922574	27.458030182850152	31.09985401155705	19.26913512636706
41	19.74311391414213	28.50212285946864	31.28096301774928	20.473800208639954
42	21.325622683121743	28.404201967049175	29.92748198955014	20.34269336027894
43	21.586050588492657	28.16684053329682	29.731342572819365	20.515766305391157
44	21.21103440475854	28.749157329706193	29.372249695299352	20.667558570235915
45	20.953136370468368	28.25092154274515	29.118220875603264	21.677721211183222
46	20.363081144870833	29.028931308047518	29.53490552419673	21.07308202288492
47	21.006710111001816	27.951206227649706	30.63614352405089	20.40594013729759
48	20.980964952356576	27.03256539344704	30.594623875137465	21.39184577905892
49	20.224682315159434	28.132464049787863	30.390597213272596	21.252256421780107
50	19.734184957386557	27.798967514967167	30.692544767556935	21.77430276008935
51	20.048037787344988	27.527229597705855	30.024956434131827	22.399776180817327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	237.0
1	604.5
2	972.0
3	8646.5
4	16321.0
5	12764.5
6	9208.0
7	7174.5
8	5141.0
9	4781.5
10	4422.0
11	4287.5
12	4153.0
13	3999.0
14	3845.0
15	3692.0
16	3539.0
17	3563.0
18	3587.0
19	3665.0
20	3743.0
21	3888.5
22	4034.0
23	4257.5
24	4481.0
25	5647.0
26	8148.5
27	9484.0
28	11358.0
29	13232.0
30	14733.0
31	16234.0
32	18560.5
33	20887.0
34	22985.0
35	25083.0
36	26776.0
37	28469.0
38	30672.5
39	32876.0
40	35430.5
41	37985.0
42	39964.0
43	41943.0
44	44069.0
45	46195.0
46	48069.5
47	49944.0
48	49940.5
49	49937.0
50	47522.5
51	45108.0
52	40822.5
53	36537.0
54	33826.0
55	31115.0
56	29141.5
57	27168.0
58	25721.5
59	24275.0
60	23438.0
61	22601.0
62	19359.0
63	16117.0
64	13244.5
65	10372.0
66	8229.5
67	6087.0
68	4926.0
69	3765.0
70	3106.5
71	2448.0
72	2027.5
73	1607.0
74	1259.5
75	670.0
76	428.0
77	377.0
78	326.0
79	239.0
80	152.0
81	124.0
82	96.0
83	68.0
84	40.0
85	34.5
86	29.0
87	22.0
88	15.0
89	9.0
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	671971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.52833534809162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1101131029323	29.294494034177067
2	8.572751826349542	6.777332180958193
3	4.03075533625917	4.779871459132867
4	2.544617713290184	4.023380092145138
5	1.7539158639809174	3.466468722188777
6	1.294974363235476	3.0712908538291983
7	1.01993610448186	2.822146346010962
8	0.8138671229763633	2.573665005263697
9	0.688296076187533	2.448647830648478
>10	5.105830565737451	36.684157394757555
>50	0.048220351377934394	1.2224980980012639
>100	0.015166078010662573	0.945810473642413
>500	3.88873795145194E-4	0.15017988505584873
>1k	7.77747590290388E-4	0.5981063794803556
>5k	3.88873795145194E-4	1.1419512447081885
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7429	1.1055536622860211	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2460	0.36608722697854523	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1431	0.2129556186204464	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	977	0.1453931791699344	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.976318918524758E-4	0.0	0.0	0.049258078101584744	0.0
2	2.976318918524758E-4	0.0	0.0	0.15878661430329583	0.0
3	2.976318918524758E-4	0.0	0.0	0.2601302734790638	0.0
4	2.976318918524758E-4	0.0	0.0	0.3672777545459551	0.0
5	2.976318918524758E-4	0.0	0.0	0.692440596394785	0.0
6	2.976318918524758E-4	0.0	0.0	1.0469201795910836	0.0
7	2.976318918524758E-4	0.0	0.0	1.3220808636086974	0.0
8	2.976318918524758E-4	0.0	0.0	1.8032028167882246	0.0
9	2.976318918524758E-4	0.0	0.0	2.0570828205383864	0.0
10	2.976318918524758E-4	0.0	0.0	2.508590400478592	0.0
11	2.976318918524758E-4	0.0	0.0	3.0156063282492847	0.0
12	2.976318918524758E-4	0.0	0.0	3.4318445290049717	0.0
13	2.976318918524758E-4	0.0	0.0	3.6337877676268766	0.0
14	2.976318918524758E-4	0.0	0.0	3.7398935370722843	0.0
15	2.976318918524758E-4	0.0	0.0	3.8701074897577423	0.0
16	2.976318918524758E-4	0.0	0.0	4.136339217019782	0.0
17	2.976318918524758E-4	0.0	0.0	4.480699315893097	0.0
18	2.976318918524758E-4	0.0	0.0	4.863453928815381	0.0
19	2.976318918524758E-4	0.0	0.0	5.1017082582432876	0.0
20	2.976318918524758E-4	0.0	0.0	5.338176796320079	0.0
21	2.976318918524758E-4	0.0	0.0	5.622415253039193	0.0
22	2.976318918524758E-4	0.0	0.0	5.9568046835354505	0.0
23	2.976318918524758E-4	0.0	0.0	6.274526728087968	0.0
24	2.976318918524758E-4	0.0	0.0	6.533764105891475	0.0
25	2.976318918524758E-4	0.0	0.0	6.776482913697168	0.0
26	2.976318918524758E-4	0.0	0.0	7.004766574748017	0.0
27	2.976318918524758E-4	0.0	0.0	7.238705241744063	0.0
28	2.976318918524758E-4	0.0	0.0	7.491097086034963	0.0
29	2.976318918524758E-4	0.0	0.0	7.7510785435681	0.0
30	2.976318918524758E-4	0.0	0.0	8.051984386230954	0.0
31	2.976318918524758E-4	0.0	0.0	8.346044695381199	0.0
32	2.976318918524758E-4	0.0	0.0	8.635045262369953	0.0
33	2.976318918524758E-4	0.0	0.0	8.916158584224616	0.0
34	2.976318918524758E-4	0.0	0.0	9.190872820404453	0.0
35	2.976318918524758E-4	0.0	0.0	9.482552074419878	0.0
36	2.976318918524758E-4	0.0	0.0	9.7736360646516	0.0
37	2.976318918524758E-4	0.0	0.0	10.086298367042625	0.0
38	2.976318918524758E-4	0.0	0.0	10.391817504029191	0.0
39	4.464478377787137E-4	0.0	0.0	10.711771787770603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	50	2.1827873E-11	45.000004	21
TCTGCGA	20	7.0311927E-4	45.0	18
ATCTCGC	20	7.0311927E-4	45.0	34
ATCCGGT	20	7.0311927E-4	45.0	11
TGTTGCG	40	6.8084773E-9	45.0	1
TAGCACG	40	6.8084773E-9	45.0	1
CGAATAT	80	0.0	45.0	14
CCGGTTA	20	7.0311927E-4	45.0	27
TACGCGG	95	0.0	42.631577	2
CGTTATT	740	0.0	42.567566	1
CTGACTA	85	0.0	42.35294	22
AGTACGG	80	0.0	42.1875	2
CGTTTTT	3205	0.0	42.121685	1
CGGTTTT	820	0.0	41.707317	1
CGTTTTA	395	0.0	41.01266	1
GATCGGT	35	6.244416E-6	38.571426	10
TGCTACG	35	6.244416E-6	38.571426	1
ATACGCG	35	6.244416E-6	38.571426	1
ATTTGCG	35	6.244416E-6	38.571426	1
TATCGCG	35	6.244416E-6	38.571426	1
>>END_MODULE